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June 15,2011
Volume 4,Issue 11
Pages -
Subject Area:Basic Subject of Animal Husbandry, Veterinary Medicine,Animal Breeding,Children and Adolescents Hygiene,Biopharmaceutics

Title: Comparison of different alfalfa varieties under drought stress

Authors: ZHU Feng, WANG Kang, DONG Kuanhu
PP: 1045-1051
Abstract: The experiment was carried out in greenhouse where the temperature and humidity were controlled. 23 alfalfa varieties were compared under drought stress to provide reference for the selection and breeding of alfalfa in drought area. The result showed that 23 alfalfa varieties were divided into three ranks of drought resistance, thereinto, ‘Ailai’, ‘kanghan NO.6’, ‘Longzhong’, ‘Chanan’, ‘Ju-3’, ‘Anxi’ and ‘Dingxi alfalfa’ had strong resistance to drought stress, and most of foreign alfalfa had the middle resistance to drought stress, so the introduction of a new variety should be acted according to circumstances.
Keywords: pratacultural science; alfalfa varieties; drought stress; cluster analysis

Title: Bioinformatics analysis of Erp from Mycobacteria

Authors: ZHAO Dong, KONG Yangyang, LI Min, WANG Jing, MA Jiali
PP: 1039-1044
Abstract: Objective: This study aimed to conduct bioinformatics analysis of the Erp orthologues from Mycobacteria. Methods: The amino acid sequences of the 11 species of Erp from Mycobacteria were analyzed with related compute software tools, and the immune function of Erp was determined and predicted on the basis of the protein database. Results: The Erp belonged to secrete protein with signal peptide took part in the establishment of cell envelope and transport and binding. Prediction of Erp antigen epitope showed the B cell predominant epitopes anchored on N terminal, while the distribution of T cell predominant epitopes was dispersed. Conclusion: Erp from Mycobacteria is a functional protein and plays important role in the immune regulation.
Keywords: basal disciplines of animal and veterinary science; Mycobacterium; nontuberculous Mycobacterium; Erp protein family; bioinformatics

Title: Association analysis on cloning, organization expression pattern and part immune traits of porcine miR-29c

Authors: HUANG Jing, XIE Shengsong, SU Lijie, ZHAO Shuhong
PP: 1033-1038
Abstract: In this study, the mature sequence of porcine miR-29c was cloned by stem-loop RT-PCR. It is widely expressed in 8 pig tissues, including heart, liver, spleen, lung, kidney, muscle, stomach, and small intestine by real-time PCR assays. One T/C polymorphism at the position 32 nt of miR-29c precursors was tested in a Landrace population by PCR-RFLP and analyzed association with immune traits. The results show that NcoⅠ enzyme genotype is significantly associated (P<0.05) with birth weight, the mean red cell volume (MCV) of 0-day-old pigs; the platelet distribution width (PLDW) of 17-day-old pigs and block of classical swine fever antibodies of 32-day-old pigs, and is more significantly associated (P<0.01) with the mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) of 0-day-old pigs, the MCHC of 32-day-old pigs. The single nucleotide polymorphism in precursor can modulate Drosha processing efficiency and thereby affect the level of miRNA expression.
Keywords: animal genetics, breeding and reproduction; porcine; miR-29c; tissues expression; traits association analysis

Title: The localization expression of TRPV6 in different intestinal segments and kidney in laying hens

Authors: YANG Junhua, ZHOU Zhenlei, DENG Yifeng, HOU Jiafa
PP: 1025-1032
Abstract: Objective: the study was aimed to investigate the distribution of transient receptor potential vanilloid receptor 6 (TRPV6) in intestine and kidney of laying hens. Methods: immunohistochemical analysis was used to investigate the TRPV6 localization and expression in different intestinal segments and kidney in laying hens during peak laying. In addition, changes of TRPV6 concentration among these tissues were obtained by the Western blotting analysis. Results: TRPV6 was localized to the brush-border membranes of the duodenum, jejunum, ileum, cecum and rectum. Expression was weaker in the rectum and little or no expression was found in crypt and goblet cells. In the kidney, distinct immunopositive staining for TRPV6 was detected at the apical domain of the proximal convoluted tubules (PCT), distal convoluted tubules (DCT) and medullary connecting tubules (CNT). Furthermore, Western blotting analysis indicated that the greatest amount of TRPV6 was expressed in the duodenum and the least in the rectum, expressing intermediate levels were in the other segments. Moreover, the kidney presented lower TRPV6 protein levels compared to duodenum. Conclusion: the epithelial Ca2+ channel TRPV6 is strongly expressed in the apical cells of the entire intestine and the renal tubules, suggesting that active Ca2+ transcellular transport plays a crucial role in dietary calcium (re)absorption in laying hens.
Keywords: veterinary medicine; laying hens; transient receptor potential vanilloid receptor 6; intestine; kidney; localization

Title: Investigation on the opinion and attitude of euthanasia among university students in Nanjing city

Authors: SUN Jing, YANG Ligang, BU Yanting, LIU Lan, JIANG Jingfeng, LIU Wenhe
PP: 1021-1024
Abstract: Objective: To investigate the opinion and attitude on euthanasia in university students. Methods: A questionnaire survey was carried out among 287 university students with the self-made questionnaire and the data were analyzed. Results: The university students’ attitude on euthanasia was positive, but few university students accepted systemic education about knowledge of euthanasia. Conclusion: It is necessary to strengthen education about euthanasia for the university students.
Keywords: nutrition and food hygiene; health education; university students; euthanasia; opinion; attitude

Title: Malignant transformation of SAEC cells induced by α particle and expression of tumor stem cells in transformed cells

Authors: ZHANG Suping, TONG Jian, Tom K HEI
PP: 1011-1020
Abstract: Objective: To establish the malignant transformation model of small airway epithelial cells (SAEC) induced by α particle and detect the expression of tumor stem cells in transformed cells. Methods: SAEC cells were exposed to different doses (0.2, 0.4, 0.8, 1.0, 2.0 Gy) of α particles by single and multiple treatment. Survival fraction was detected using plating efficiency immediately after the irradiation. Cell transformation represented by resistance to serum and N-(phosphonoacetyl)-L-aspartate (PALA), cell proliferation, independent growth in soft agar, invasion ability and tumor growth in nude mice was measured. CD338 and CD133 were detected by flowcytometry and immunohistochemistry. Results: Survival fraction showed a dose-dependent manner of cytotoxicity of α particle on SAEC cells. Serum resistance and PALA resistance were observed in the process of transformation. All the cells in the irradiated groups could form colonies in soft agar, and with an increased invasion ability in the transformed cells. Three out of the five nude mice in the 0.8 Gy multiple treatment group formed tumors. CD338 and CD133 were increased in transformed SAEC cells and in tumor of nude mice. Conclusion: Human SAEC cells could be malignantly transformed in vitro by α particle and could form tumor in vivo in nude mice. Tumor stem cells increased in transformed cells.
Keywords: radiation medicine; radiation carcinogen; α particle; small airway epithelial cells; malignant transformation; tumor stem cells

Title: A survey on incidence of common diseases among school age children in Lanzhou city in 2008

Authors: LI Jing, ZHANG Xiaoling, WEI Yong, WANG Yu
PP: 1007-1010
Abstract: Objective: Survey on incidence of common diseases among school age children in Lanzhou city in 2008, and provide scientific evidence for their health care work. Methods: The method of cluster sampling was used to randomly select 17 572 children come from the urban area and the suburban area, and the physical examination data of these students were analyzed and evaluated, including height, weight, eyesight, caries, HBsAg. Results: The prevalence rates of poor visual sight, malnutrition, caries and the positive rate of HBsAg were 55.16%, 21.73%, 13.28%, 0.67%, respectively. The morbidity of the mild and moderate low eyesight in the urban was significantly higher than that in the rural. The severe low eyesight, malnutrition, caries and positive rate of HBsAg in the rural were higher than that in the urban. Conclusion: The prevalence of low eyesight was higher in city pupils, the incidences of severe low eyesight, malnutrition, caries and positive rate of HBsAg in the rural were higher, while strengthen health education on visual acuity protection, the work of health care in the rural school should be made better as well.
Keywords: hygiene of children and adolescents; eyesight; nutritional status; caries; school children

Title: Impact of food safety incident on children’s health behaviors from the vision of ecology

Authors: SHEN Jie, ZHANG Peng, SUN Guiju, YANG Jin
PP: 1002-1006
Abstract: The happening of food safety incident has an inestimable bad impact on the healthy development of children, which has given rise to wide attention in all trades. Based on the ecological system theory, this paper analyzes and discusses the impact of food safety incidents on children’s health behaviors from three levels, namely individual, family and society. In order to boost the healthy development of children, it is necessary to create a healthy and safe environment for adolescents, take the impact caused by lots of factors seriously, pay attention to the mutual relations among elements and enhance the coordination of all sides.
Keywords: hygiene of children and adolescents; children; health behavior; food safety; ecological system theory

Title: Effects of Tongmai Dasheng tablet on MMP-9 about rat renal deficiency induced ovulatory infertility

Authors: MA Jie, FU Chunhua, HUANG Jinzhu, FANG Yunyun
PP: 997-1001
Abstract: Objective: To explore the matrix metalloproteinases 9 (MMP-9)’s change of androgen-sterilized rats (ASR) before and after Tongmai Dasheng tablet treatment. Methods: ASR was induced, and MMP-9 expression was detected with situ hybridization. Results: Because of drug intervention, the concentration and total amount of expression in each drug group were much greater than that in model group. The expression of MMP-9 in the high dosage group of Tongmai Dasheng tablet was as high as that in the control group (P＞0.05). The expression of MMP-9 in the high dosage group of Tongmai Dasheng tablet was much higher than that in clomiphene citrate group (P<0.05). Conclusion: To rat renal deficiency induced ovulatory infertility, Tongmai Dasheng tablet can promote the expression of MMP-9 and play a role in the treatment of infertility. This may be one of the mechanisms on promoting ovarian follicle development and ovulation.
Keywords: gynecotokology; Tongmai Dasheng tablet; matrix metalloproteinase-9; ovulation disorder; infertility; androgen-sterilized rats model

Title: Analysis on kinematics of a multi degree of freedom prosthesis

Authors: SUN Gaozuo, FAN Binghui
PP: 992-996
Abstract: The structure of a robot-prosthesis is designed with five movement degree of freedom (DOF) and a local DOF. By analysis of the structure, its coordinates of all the links are established using D-H method. The matrix equation of the kinematics relative to the foundation coordinates is derived. On this basis, by dint of Monte-Carlo method, the work space of the prosthesis is analyzed, and simulated in graphic way which shows a scarce ellipsoid shape. The work space is compact, and does not have any holes or sockets.
Keywords: rehabilitation medicine; robot; prosthesis structure; kinematics analysis; work space analysis

Title: 15-alkylidene andrographolide derivatives and their cytotoxic activity

Authors: XU Haiwei, LIU Gaizhi, ZHU Songlin, DAI Guifu, LIU Hongmin
PP: 983-991
Abstract: In order to investigate the cytotoxic activity of 15-modification andrographolide derivatives, a series of 15-alkylidene andrographolide derivatives were synthesized by dehydration and condensation reactions with andrographolide(1) as the starting material. Their cytotoxic activity to Ec9706 was also investigated. The results showed that some of them had the cytotoxic activity to Ec9706. Compound 5b was the best one with the IC50 of 13 mg/L, which was better than that of andrographolide(1) and 11,12-didehydroandrographolide(2).
Keywords: medicinal chemistry; andrographolide; 15-alkylidene andrographolide derivatives; cytotoxic activity

Title: Design, synthesis and structure-activity relationship of novel PTP1B inhibitors

Authors: TONG Yuanfeng, ZHANG Pei, TIAN Jinying, YE Fei, WU Song
PP: 975-982
Abstract: Starting from the natural product petroselinic acid as lead compound, N-(2-tetradecanoxyphenylaminocarbonyl) benzoic acid was found as a small molecule protein tyrosine phosphatase 1B (PTP1B) inhibitor. For further modification, a series of (2-tetradecanoxyphenylaminocarbonyl) benzoic acid derivatives were designed and synthesized. The inhibitory activities of these compounds in vitro against PTP1B were evaluated. The results indicated that most of the derivatives showed more potent activities against PTP1B and IC50 of some compounds arrived at 100 nm in vitro. The structure-activity relationship (SAR) was summarized.
Keywords: medicinal chemistry; (alkoxyphenyl)-aminocarbonyl benzoic acid derivatives; synthesis; structure-activity relationship; protein tyrosine phosphates 1B

Title: cDNA cloning and function characterization of Artemisia annua L. genes encoding cytochrome P450 monooxygenase and P450 oxidoreductase

Authors: KONG Jianqiang, ZHENG Haina, ZHI Xiaohui, SHI Yanwei, WANG Wei, ZHU Ping, LIU Zhenya
PP: 967-974
Abstract: The total RNA of Artemisia annua L. plant was extracted and transcripted into cDNA. Two genes encoding amorpha-4,11-diene cytochrome P450 monooxygenase (CYP71AV1) and its redox partner NADPH: cytochrome P450 oxidoreductase (CPR) were cloned from the Artemisia annua L. cDNA. All cloned cDNAs were confirmed by DNA sequencing. A Saccharomyces cerevisiae expression vector pYG450Gre containing CYP71AV1 and CPR genes was constructed. pYG450Gre, pGBT9/A/HMG/G/FPP and pGADADS were co-transformed into S.cerevisiae WK1 to form the engineered yeasts producing artemisinic acid by LiOAc-mediated method. The identification of positive clone and the fermentation of the transformants were performed. The fermentation metabolites were also extracted and analyzed by high performance liquid chromatography (HPLC) and Fourier transform ion cyclotron resonance mass spectrometry. The result showed the identification of artemisinic acid from engineered yeast compared with the artemisinic acid standard, which indicated the CYP71AV1 and CPR genes function in engineered S. cerevisiae. These results were not yet reported in China.
Keywords: microbial medicine; cytochrome P450 monooxygenase; NADPH: cytochrome P450 oxidoreductase; artemisinic acid; engineered Saccharomyces cerevisiae

Title: Study on pharmacokinetics of carbamazepine in the small intestine based on remote controlled capsule

Authors: JIA Ziru, LIU Hongying, PI Xitian, ZHENG Xiaolin, TANG Wei
PP: 963-966
Abstract: Objective: To investigate the pharmacokinetics of carbamazepine (CBZ) in the proximal small intestine of healthy volunteers and obtain the standard pharmacokinetic parameters. Methods: 200 mg CBZ was delivered in the proximal small intestine of healthy volunteers by remote controlled capsule system, which made by our work group. The concentration of CBZ in serum was determined by TDx. The main parameters were calculated by 3P87 pharmacokinetic program. Results: The CBZ pharmacokinetics conforms to a one-compartment open model after a single delivered dose of CBZ (200 mg) in upper small intestine of healthy volunteers. The main parameters were as follows: Cmax was (2.1±0.3) mg·L-1, Tmax was (8.6±2.4) h，T1/2 was (6.9±1.6) h, AUC(0~36) was (33±8) mg·L-1·h-1. Conclusion: The pharmacokinetics of CBZ showed that there were slow and good absorption in upper small intestine of healthy volunteers.
Keywords: biological pharmacology; carbamazepine; remote controlled capsule; intestinal absorption; pharmacokinetics

Title: Research advances of insulin pump and insulin used in pump

Authors: GONG Hao, HUANG Kun
PP: 955-962
Abstract: This paper summarizes the progresses on insulin pump and insulin analogues or formulations used in the pump therapy. Continuous subcutaneous insulin infusion (CSII) has shown obvious advantages compared to other methods of administration. Clinical problems and technique bottlenecks of this method, development trends of insulin pump closed-loop system and main features of insulin used by pump are analyzed, its future trends are also provided.
Keywords: pharmacology; diabetes; reviews; insulin pump; insulin used in pump

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