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December 15,2010
Volume 3,Issue 23
Pages -
Subject Area:Chinese and Western Integrative Medicine,Health Preserving and Rehabilitation of Traditional Chinese Medicine,Basic Theories of Traditional Chinese Medicine,Chemistry of Traditional Chinese Medicine,Biomedical Engineering,Human Physiology

Title: Experimental study on curative effect of Qingganyixian decoction on hepatic fibrosis

Authors: JIA Aiqin, YIN Changjian
PP: 2455-2460
Abstract: Objective: To observe the preventive and therapeutic effects on rat hepatic fibrosis of Qinganyixian decoction, and discuss its mechanism of action. Methods: Divide 110 Wistar rats into 9 groups. The control group was given normal diet, free drinking water. Give the remaining groups subcutaneous injection with 40% CCl4-oil solution twice a week, and lavage them the herbs or water for 8 weeks. Detect the liver function and hepatic fibrosis indexes. Detect liver tissue homogenate malondialdehyde (MDA) content and superoxide dismutase (SOD) activity, assay the content of TNF-α, TGF-β1, MMP-2 and TIMP-2, detect the expression of TGF-β1 mRNA using RT-PCR, and test the apoptosis of HSC with terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL) step detection. Results: 1) Qinganyixian decoction can significantly decrease the liver inflammation and lipid peroxidation, where Qingrejiedu herbs and Liangxuehuoxue herbs play the most important role. 2) Qinganyixian decoction can decrease the expression of α-SMA positive cells significantly, where Qingrejiedu herbs and Liangxuehuoxue herbs play the most important role. 3) Qinganyixian decoction can reduce the expression of TGF-β1 from mRNA to protein level significantly. 4) Qinganyixian decoction can significantly reduce the TNF-α level, especially the Qingrejiedu herbs. 5) Qinganyixian decoction can reduce TIMP-2, where Liangxuehuoxue herbs play the major role. Conclusion: Qingganyixian decoction can prevente and treate hepatic fibrosis by improving liver inflammation, inhibiting the activation of HSC and promote apoptosis of activated HSC, and reducing the expression of hepatic fibrosis promoting factors, wherein Qingrejiedu herbs and Liangxuehuoxue herbs play the most important role.
Keywords: medicine combined with Chinese traditional medicine and western medicine; Qingganyixian decoction; liver fibrosis; prevention and treatment; experimental study

Title: Comparison of integrated diabetes mellitus and kidney deficiency syndrome between monozygotic and dizygotic twins

Authors: TAN Conge, NI Qing, ZHANG Tiane, FENG Wenzhe, CHEN Kang, WANG Miqu
PP: 2449-2454
Abstract: Objective: To compare the similarities and differences of both disease and TCM syndrome between monozygotic (MZ) and dizygotic (DZ) twins, and further explore the genetic characteristics of type 2 diabetes mellitus (T2DM) with kidney deficiency syndrome. Methods: Firstly, three pairs of twins were screened from T2DM pedigrees through clinical investigation and laboratory tests (fasting blood glucose, glucose tolerance tests and glycosylated hemoglobin). Secondly, the zygosity of each pair of twins was determined through the method of twin’s phenotype similarity comparison. Finally, the kidney deficiency syndrome of 3 pairs of twins was decided by traditional Chinese medicine (TCM) experts and questionnaire scores. Results: Among three pairs of twins, two pairs were MZ and one pair was DZ. Two pairs of MZ twins suffered with both T2DM and kidney deficiency syndrome. One of DZ twins was diagnosed with T2DM and kidney deficiency syndrome and the other was diagnosed with no kidney deficiency syndrome but impaired glucose tolerance (IGT). Conclusion: The differentiated results of 3 pairs of twins suggest that kidney deficiency syndrome is liable to be accompanied with T2DM. There is consistency of suffering T2DM and kidney deficiency comsyndrome between MZ twins although the differences in severity of illness are obvious.
Keywords: medicine combined with Chinese traditional medicine and western medicine; diabetes mellitus; kidney deficiency syndrome; monozygotic twin; dizygotic twin

Title: Specificity of objective thinking form electroencephalogram based on β frequency range power spectra

Authors: WEI Yulong, LIU Tianjun
PP: 2445-2448
Abstract: This paper discovers the significant differences between objective thinking and imaginative thinking, in the power spectrum change course of different thinking subjects of the three thinking forms including objective thinking, imaginative thinking and abstract thinking measured by electroencephalogram (EEG) synchronous measurement technique, and provides the specificity of objective thinking form based on β frequency range.
Keywords: healthcare and rehabilitation of traditional Chinese medicine; objective thinking; β frequency range; electroencephalogram; specificity

Title: Effects of qi-benefiting, yin-fostering, blood- invigorating and diuresis-activating traditional Chinese medicine on expression of MMP-2 in retinal tissue after retinal detachment of rabbit

Authors: LIU Ping, PENG Jun, PENG Qinghua, YAO Xiaolei
PP: 2437-2444
Abstract: Objective: To investigate the effects of Fuming tablets (FMT) of qi-benefiting, yin-fostering, and blood-invigorating and diuresis-activating on the expression of matrix metalloproteinase-2 (MMP-2) in the retinal tissue after detachment of the retina of rabbit. Methods: 36 rabbits were divided into normal contrast group (group A), retinal detachment model group (group B), western medicine contrast group (group C), and FMT group (group D). Each group had 9 rabbits. Group B, C, D were made retinal detachment model. After models were made one day they were given medicine. Group A and B were administrated intragastrically with 5 mL/kg warm water, once a day. Group C was administrated intragastrically with 5 mL/kg western medicine solution, once a day. Group D was administrated intragastrically with 5 mL/kg FMT suspension, once a day. Then the method S-P was adopted to investigate the expression of MMP-2, MMP-2 in the retinal tissue respectively in the 7th day, 14th day and 21st day. Results: MMP-2 took on strong positive expression in the RPE cells of model group and west medicine group, dyeing dark or pretty dark color; while weak masculine expression in the RPE cells of FMP group, dyeing shallow color. Conclusion: FMT with the functions of qi-benefiting, yin-fostering, blood-invigorating and diuresis-activating, can decrease the expression of MMP-2 in the retinal tissue.
Keywords: otorhinolaryngology of Chinese medicine; qi-benefiting yin-fostering and blood-invigorating and diuresis-activating method; Fuming tablets; retinal detachment model; matrix metallo-proteinase-2; rabbit

Title: Effects of ligustrazine combined with benazepil on the expression level of soluble intercellular adhesion molecule-1 in GK rats’ nephridial tissue

Authors: YIN Liping
PP: 2432-2436
Abstract: Objective: To observe the effects of ligustrazine combined with benazepil on the expression level of soluble intercellular adhesion molecule-1 in Goto-Kakizaki (GK) rats’ nephridial tissue with diabetic nephropathy. Methods: Forty GK male rats were randomly divided into four groups: model group, ligustrazine group, benazepil group and ligustrazine combined with benazepil group, 10 every groups. GK rats received corresponding medicines according to the experimental design for 16 weeks. The rats were put into metabolism cages in the 8th week, the 12th week and the 16th week, collecting urine to detect proteinuria in 24 h. After treating for 16 weeks, ICAM-1 was detected by immunohistochemistry, the level of TNF-α by radioimmunoassay and IL-6 by enzyme-linked immuno sorbent assay (ELISA) in blood serum. Results: At the end of experiment, the level of Scr, BUN, proteinuria in 24 h, TNF-α, IL-6, ICAM-1 were all decreased in all therapeutic groups compared with model group (P＜0.05 or P＜0.01), ligustrazine combined with benazepil group in particular (P＜0.01). Ccr was increased in all therapeutic groups compared with model group (P＜0.05). Ligustrazine combined with benazepil group can down regulated the expression of sICAM-1, decrease the level of Scr, BUN, TNF-α and IL-6, and reduce the excretion of proteinuria to improve renal function in GK rats with diabetic nephropathy. Conclusion: ligustrazine combined with benazepil can improve renal function in GK rats with diabetic nephropathy inflammation change of nephridial tissue. To degrade the express level of ICAM-1 and reduce the level of TNF-α, IL-6 is one of the possible mechanisms.
Keywords: internal medicine of traditional Chinese medicine; ligustrazine combined with benazepil; tumor necrosis factor-α; intercellular adhesion molecule-1; inflammation of nephridial tissue; experimental study

Title: A new physics model of meridian system in traditional Chinese medicine

Authors: LI Pengfei, CHAI Lihe
PP: 2426-2431
Abstract: This paper takes life as information process. Based on a new entity, the general information could be defined, the reality of meridian can be revealed from a new view, and then the maximum flux principle（MFP）is proposed, the meridian equation that controls the evolution of human body is deduced to show the visual image of meridian system. In the end of this paper, by a practical case, this theoretical framework can be used to show the evolution properties of meridian, and prove that this theoretical framework both has important meanings in revealing the basis of traditional Chinese medicine (TCM) and play a guiding role in regulating the meridian system.
Keywords: the basic theory of traditional Chinese medical; meridian; complex system; information

Title: Study on relationship between pollination characteristic and sterility of Liriope spicata var. prolifera

Authors: ZHOU Jianqiu, ZHOU Qun, WANG Xiaogang, WANG Jingjing, CHEN Jiachun
PP: 2421-2425
Abstract: In this paper, the relationship between pollination characteristic and sterility of Liriope spicata var. prolifera was studied. The results were as follows: Duration of flowering of L. spicata var. prolifera varied from late June to late August．Anthesis of a single flower often lasted for 1 d．L. spicata var. prolifera bloomed at around 9: 20 and finished at around 16: 30. The stigma receptivity was the highest 1 to 2 hours after blooming, although stigma had catalase activity in the whole anthesis. The pollen count was 25 778±9 682. The pollen-ovule ratio, ranging from 4 296±1 614, reflected as an obligate xenogamy system. Pollen in the anthesis had no viability and their aberration rate was 95.55%. Wind pollination did not exist in this area. The flowers of this species were pollinated by insects. Besides low frequency and efficiency of pollination, the high aberration rate and debility of pollen were significant reasons of sterility of L. spicata var. prolifera.
Keywords: pharmacognosy; Liriope spicata var. prolifera; pollination; pollen viability; stigma receptivity; sterility

Title: Analysis of alkaloids in Aconitum soongaricum Stapf of Xinjiang

Authors: CAI Dongmei, LIU Yue, ZHANG Fan
PP: 2416-2420
Abstract: Advanced methods and techniques were used to extract the total alkaloids in different parts of Aconitum soongaricum Stapf. Then thin layer chromatograph (TLC) was used to preliminarily identify main chemical components of the alkaloids, and ultraviolet spectrum (UV) was used to confirm the components and the contents of alkaloids in root, stem and leaf. Experimental results show that napelline, 12-epi-napelline, songorine, neoline and songoramine are identified in A. soongaricum Stapf; also, napelline and songoramine are first found in it. And the contents of total alkaloids are 1.67, 0.05, 0.29 mg/g in root, stem and leaf, respectively. These establish foundations for further research, including pharmacology, toxicity and new drug development.
Keywords: chemistry of traditional Chinese medicine; Ranunculaceae; Aconitum soongaricum Stapf.; diterpenoid alkaloid

Title: Study on metabolism of arctiin with human intestinal flora

Authors: ZHANG Jiankui, JIANG Hong, YUAN Yuan, KANG Tingguo
PP: 2412-2415
Abstract: In this paper, metabolic transformation of arctiin with human intestinal flora was studied. By methods of silica gel and SephadexLH-20 column chromatography, two metabolites were separated from human fecal suspension; and by spectral analysis their structures were identified as arctiin I, arctigenin Ⅱ and (8R,8R′)-3,4-dihydroxy-4′,5′-dimethoxy-9-oxo-8-8′,9-O-9′-lignan Ⅲ. Arctiin can be metabolized by human intestinal flora. The metabolic pathway of arctiinⅠin human intestinal may be: the glucosidic bond of compoundⅠbroke thereby compound Ⅱ was obtained; and compound II separated —CH3 from the third position thereby compound Ⅲ was obtained.
Keywords: chemistry of traditional Chinese medicine; intestinal bacteria metabolism; arctiin; arctigenin

Title: Study on water extraction technology for Chuankeping granules

Authors: LI Jing, ZHAO Jing, LI Junge, HUANG Juan, LV Xia, LIU Qian
PP: 2408-2411
Abstract: The herbs of the prescription were divided into alcohol extract part and water extract part on the basis of traditional Chinese medicine (TCM) formula principle and researches of modern Chinese herbal medicine chemistry, for making the Chuankeping granules easier to take and to carry. The purpose of this test was to investigate water extraction process of the Chuankeping granules. The orthogonal test design was chosen, with the rate of harvesting of the extract and the contents of baicalin were used as indices of appraisa1. And the relevant data were got through the weight method and high performance liquid chromatography (HPLC) measurement. Then comprehensive evaluation method was used to study the extraction time, amount of water and extraction times of three factors, three levels on the impact of water extraction, to optimize the best extraction process. The results showed that the crude drug which had been soaked in water for 0.5 h should be extracted three times, by tenfold amount of water and 1.0 h with each. The three validation tests showed that the optimized extraction process was simple, reasonable, reliable and practical.
Keywords: Chinese pharmacy; Chuankeping granules; baicalin; orthogonal test

Title: Effect of Zuoguiwan medicated serum on change of ALPase content during differentiation into osteoblast from rat marrow mesenchymal stem cells

Authors: XU Lingxiao, GAO Jun, ZHANG Qiande
PP: 2402-2407
Abstract: Objective: To study the change of ALPase during its differentiation into osteoblasts from rat marrow mesenchymal stem cells induced by Zuoguiwan medicated serum. Methods: Using the method of adherent culture to culture marrow mesenchymal stem cells and then purifying mesenchymal stem cells for 3 times with selective plating technique. Conventional induction group uses β-glycerophosphate, dexamethasone, and vitamin C to induce mesenchymal stem cell differentiate into osteoblasts, and the experimental group adds Zuoguiwan medicated serum on the basis of the conventional induction group. Alizarin red was used to stain calcium nodules and alkaline phosphatase staining was used to identify the successful induction. Colorimetric assay was used after 0, 7, 14 and 21 days respectively to measure alkaline phosphatase content. Results: Whole bone marrow adherent and selective plating technique were combined to get the purified mesenchymal stem cells. Alkaline phosphatase measured by colorimetry increased with the prolongation of induction. Conclusion: Rat mesenchymal stem cells induced by the drugs can differentiate into osteoblasts, Zuoguiwan medicated serum can promote the express of alkaline phosphatase in the induction process.
Keywords: pharmacology of traditional Chinese medicine; mesenchymal stem cells; Zuoguiwan medicated serum; osteoblasts; alkaline phosphatase

Title: Advances in Rhizoma et Radix acanthopanacis research

Authors: LI Jianyin, YANG Yongjian
PP: 2397-2401
Abstract: This paper collected a large number of research and literature about Rhizoma et Radix acanthopanacis over the years, summarized and analyzed the research, and strived to find out the reasons for its endangerment from the biotope, seed development, spore variation, phenology and other aspects of reproductive ecology; and to find out the similarities and differences between the Rhizoma et Radix acanthopanacis and Radix Acanthopanacis semticosi from the contained chemical composition and pharmacological effects. The comparative analysis on the research achievement of Rhizoma et Radix acanthopanacis over the years shows that the main reason of Rhizoma et Radix acanthopanacis endangerment is the biotope, Rhizoma et Radix acanthopanacis and Radix Acanthopanacis semticosi have the same chemical composition and pharmacologic effects, so it may be as a substitute for Radix Acanthopanacis semticosi. Through the analysis and research above, the paper offers reference to further exploration of Rhizoma et Radix acanthopanacis and sustainable utilization of its resource.
Keywords: Chinese materia medica; endangered plants; reviews; Acanthopanax brachypus Harms

Title: Pharmacodynamics research on method of stir-baking medicine with salt solution in traditional Chinese medicine processing

Authors: RONG Jie, ZHANG Junping
PP: 2392-2396
Abstract: The method of stir-baking medicine with salt solution is a traditional processing method for Chinese medicine, which can lead medicine to kidney and strengthen functions of medicine, such as warming kidney to invigorate yang, relieving pain, arresting polyuria, checking diarrhea and nourishing yin, as well as modern pharmacologic actions, like lowering blood glucose and regulating bone metabolism etc., and alleviate toxic dryness of medicine according to the pharmacodynamics research, resulting in good curative effect of medicine.
Keywords: pharmaco-preparing process; method of stir-baking medicine with salt solution; reviews; pharmacodynamics

Title: Study on immunosuppression of Glucosida Tripterygii in heart-transplanted rats

Authors: ZHOU Yun, YAN Zhiguo, YANG Shaojun, YANG Dakuan
PP: 2389-2391
Abstract: Objective: To study the immunosuppression of Glucosida Tripterygii in heart-transplanted rats. Methods: Male SD rats served as donors and Wistar as recipients, heart transplantation was performed by renal vein cuff technique. Thirty six recipients were divided into two groups at random: control group and Glucosida Tripterygii treated group (30 mg·kg-1·d-1 ). Transplanted heart beating intensity and beating time were observed. The level of IL-2 in recipients’ serum was measured by ELISA method. Transplanted heart were collected and examined by HE stain, when they did not beat. Results: In Glucosida Tripterygii treated group, transplanted heart beating intensity was stronger, beating time was longer, the concentration of IL-2 in serum was lower, and rejection was obviously mild than them in control group. Conclusion: Glucosida Tripterygii can suppress the rejection in heart-transplanted rats.
Keywords: medicine laboratory animal science; Glucosida Tripterygii; heart transplantation; immunosuppression

Title: Repeatability of the short-term frequency domain parameters of heart rate variability

Authors: ZHANG Li, TIAN Xuelong, LIU Hanlin
PP: 2384-2388
Abstract: Pearson correlation coefficient and intraclass correlation coefficient (ICC) are used to study the short-term and long-term repeatability of the short-term frequency domain parameters of heart rate variability (HRV) with 20 healthy volunteers. The results show that the short-term frequency domain parameters of HRV have good short-term and long-term repeatability, and the short-term repeatability is better than the long-term repeatability, which provides a basis for HRV used in clinical testing and research.
Keywords: biomedical engineering; heart rate variability; Pearson correlation coefficient; intraclass correlation coefficient; repeatability

Title: Effect of compound pachymic acid on peripheral blood lymphocytes mitochondrial transmembrane potential in rats in vivo

Authors: ZHANG Xuefeng, LIU Hongyu
PP: 2381-2383
Abstract: Objective: To study the effect of pachymic acid (PA), a natural triterpenoid, to peripheral blood lymphocytes (PBLs) mitochondrial transmembrane potential in rats. Methods: In this study, PBLs mitochondrial transmembrane potential were measured in rats 7 days after intragastric administration using different dose PA ［1.0, 10.0 （mg/kg）·d-1］, CsA［5.0 （mg/kg） ·d-1］ and olive oil as control group. Results: Data suggested that PA treatment protect PBLs mitochondrial transmembrane potential from depolarization. Large dose of PA ［10.0(mg/kg)·d-1］has better pharmacological effect than small dose of PA ［1.0 (mg/kg)·d-1］ .Conclusion: The findings indicate that PA has a protective effect acting to PBLs through a novel mechanism involving stabilization of the PBLs mitochondrial transmembrane potential.
Keywords: pharmacology; pachymic acid; mitochondrial transmembrane potential; intragastric administration; peripheral blood lymphocytes

Title: Mechanism of down-regulated CFTR expression by ozone stress on human bronchial epithelial cells

Authors: QU Fei, QIN Xiaoqun1, XIANG Yang1, TAN Yurong1, LIU Huijun
PP: 2376-2380
Abstract: Objective: To investigate the signal mechanism of cystic fibrosis transmembrane conductance regulator (CFTR) expression by ozone stress on bronchial epithelial cells (BECs). Methods: Real-time polymerase chain reaction (PCR), immunofluorescence and western blot were detected the expression of CFTR after ozone stress. Results: Real-time PCR showed that pretreatment with AG490 attenuated the down-regulated CFTR mRNA expression induced by ozone stress. Immunofluorescence and western blot revealed that pretreatment with AG490 raised the CFTR protein expression. And it was also observed that ozone stress accelerated the phosphorylation of signal transducer and activator of transcription 1 (STAT1) in BECs. Furthermore, pretreatment with inhibiters, reactive N-acetylcysteine (NAC), amino guanidine (AG) and zinc protoporphyrin IX (ZnPP-9) could cut down the phosphorylation of STAT1. NAC and AG could down-regulate the expression of CFTR after ozone stress by immunofluorescence and western blot, but not ZnPP-9. Conclusion: NO and reactive oxygen species (ROS) can effect the expression of CFTR through STAT1 signal.
Keywords: physiology; cystic fibrosis transmembrane conductance regulator; ozone stress; signaling molecule

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