Home > Highlights of Sciencepaper Online > Current Issue

 
 
 
ISSN 1674-2850
CN 11-9150/N5
 
Current Issue
Previous Issue  |  Next Issue
October 15,2010
Volume 3,Issue 19
Pages -
Subject Area:Genetics,Biophysics,Enzymology
 
Title: Effects of vibration stimulation on muscle fiber’s morphology and structure of rats with skeletal muscle disuse atrophy
Authors: HUANG Peng, WEI Hongwen, YE Jing, JIAO Wei
PP: 2055-2063
Abstract: Objective: To study counteracting effects of vibration stimulation (VS) on skeletal muscle disuse atrophy (SMDA) in rats soleus muscles. Methods: 40 female SD rats were assigned randomly to one of the four groups: simultaneous control (A), suspension plus vibration stimulation (B), suspension (C), and suspension control (D). Soleus muscles were examined 14 days later. Sections were treated with mATPase stain. The cross sectional area (CSA) of fibers, the relative proportion of types I and II were measured using Leica image analysis system. Results: Compared with those of group A, the wet weight/body of soleus in group C were significantly reduced, while the relative proportion of type I fibers was reduced and type II fibers increased. Compared with group C, the wet weight/body of soleus recovered completely in group B&D, and the CSA of type I fibers recovered partly in group B. The relative proportion of type I fibers recovered in group B (P<0.05). Conclusion: The present study showed that VS is effective in counteracting the disuse muscular atrophy induced by simulated weightlessness.
Keywords: sport hygiene; vibration stimulation; skeletal muscle disuse atrophy; proportion of type I/II fibers; wet weight
 
Title: Research on physical function evaluation system of pre-competition training of university orienteering athletes
Authors: DAI Jianghong, SONG Weihong, CAO Jin, DAI Yanpeng, ZHENG Sheng, LUO Hui
PP: 2049-2054
Abstract: In order to find and explore the index system of functional evaluation in pre-competition training of university orienteering athletes, the physiological and biochemical indexes of nine athletes who attended orient cross-country tournament of Hunan province were examined and measured in the process of pre-competition training. Results: 1) In the training process of orient cross-country sport, the change of hematocrit (Hct), hemoglobin (Hb) and red blood cell (Rbc) was not so apparent. 2) The change tendency of blood urea nitrogen (BUN) in the athletes’ serum was significantly different. It rose first and decreased later. 3)Blood ammonia (BA) had taken place in a significant change, there has been increased after the first change in the trend of lowering. 4) The athletes’ maximal oxygen (VO2max) increased dramatically. Conclusion: The Hct, Hb and Rbc in blood are not with the specificity in evaluating the athletes’ physical function; the BUN in the athletes’ serum can accurately evaluate their physical function in the process of pre-competition training; BA can precisely evaluate the functional state of the body of athletes; and the significant increasing of the athletes’ VO2max shows that the training plan is reasonable.
Keywords: human movement science; function evaluation; pre-competition training; orienteering
 
Title: Characteristics research and analysis on age, height, weight and Quetelet index of elite women sprinter between domestic and foreign athletes
Authors: LONG Jiayong, LIANG Jianping
PP: 2043-2048
Abstract: Through using the method of literature investigation, mathematical statistics, comparative study and so on, four basic parameters, namely the age, height, weight and Quetelet index of elite female sprinter at home and abroad were researched and analyzed. The result showed that there were significant differences (P<0.05) in age of 100, 200 meters domestic outstanding female sprinter, but no difference (P>0.05) in other basic parameters. There was no difference (P>0.05) in the four basic parameters of foreign outstanding female sprinter. Overall, the structural features of four basic parameters of both were generally in line with the normal distribution respectively, indicating that the age, height, weight and Quetelet index of elite female sprinter at home and abroad reached a more relatively stable state and balance respectively. There were a very significant difference (P<0.01) in average age and significant differences (P>0.05) in average weight and Quetelet index between domestic and foreign outstanding female sprinter, Compared with foreign ones, the average age of domestic outstanding female sprinter is a little bit smaller, average weight and Quetelet index are relatively low.
Keywords: athletic training; elite sprinter; women; age; height; weight; Quetelet index
 
Title: Body composition and bone density in female wrestlers and controls
Authors: LI Hongjuan, CHEN Delong
PP: 2037-2042
Abstract: Objective: The purpose of the present investigation was to describe the body composition and bone density of female freestyle wrestlers comparing with controls. Methods: 26 female wrestlers and 30 controls recruited from Beijing Sport University were evaluated for measurements of body composition and bone density by dual-energy X-ray absorptiometry (DEXA). Results: Comparing to the control group, the wrestlers had higher lean mass and bone mineral mass, and the average bone density of wrestlers was greater than controls significantly, whereas body fat mass and body fat percent were lower than controls (P<0.05). In almost all parts of body (except for head), the percentage of fat composition in wrestlers was about half of that in controls. Conclusion: The characteristics of body composition and bone density in female wrestlers might be related to chronic training. The special standard of bone density for athletes might be required. Body composition and energy balance of female athletes should be paid close attention, especially for their effect on reproductive function of female athletes.
Keywords: sports medicine; female; wrestler; body composition; bone density
 
Title: The application of experience element analysis techniques in the treatment for students’ emotional disorders
Authors: WANG Rufang
PP: 2034-2036
Abstract: Objective: To explore the role of experience element analysis techniques in the treatment of students’ emotional disorders. Methods: Applied the experience element analysis techniques to treat 40 cases of university students with emotional disorders in strict accordance with the procedures, and conducted pre-test and post-test with the self-rated health emotional scales (SHES) and the state-trait anxiety inventory (STAI). Results: The results showed that the two scale scores were significantly different before and after treatment. Conclusion: The experience element analysis techniques have significant improvements to the students’ emotional disorders, and increase their positive emotion, reduce their negative emotion and anxiety level.
Keywords: applied psychology; experience element analysis technology; students’ emotional disorders; psychotherapy
 
Title: Initial survey on Agrobacterium-mediated SOS1 gene transformation of tobacco and salt tolerance test of transgenic tobacco
Authors: ZHANG Junfang, YUE Yuesen, ZHANG Jiachang
PP: 2027-2033
Abstract: SOS1 gene, which is a salt overly sensitive (SOS) mutant, was transferred into tobacco by Agobacterium-mediated transformation. The polymerase chain reaction (PCR) analysis indicated that SOS1 gene had been inserted into tobacco genome in some T0 generation transgenic plants. The callus, cells, and seedlings were detected in some T3 generation transgenic plants, confirmed that transgenic tobacco had greater ability to tolerate salt stress compared to wild type (WT) plants.
Keywords: genetics; Agrobacterium-mediated transformation; salt tolerance; tobacco; SOS1
 
Title: Effects of seeds soaking with exogenous glycinebetaine on the growth of cucumber seedlings under salt stress
Authors: SHI Jie, SHI Bing, XUE Cheng, YU Lijuan, FENG Huyuan
PP: 2021-2026
Abstract: Effects of seeds soaking with different concentrations of exogenous glycinebetaine on the growth of cucumber (Cucumis sativus L.) seedlings under different concentrations of neutral salt (NaCl) and alkaline salt (Na2CO3) stress were researched, using “Jinlv-3” as the experimental material and vermiculite with nutrient solution culture. It was found that seeds soaking with exogenous glycinebetaine increased the leaf fresh weight, root fresh weight, root length of cucumber seedlings in a certain degree under salt stress, and the best glycinebetaine soaking concentration of 20 mmol/L was found under high salt stress. But it had no significant beneficial effects on the chlorophyll content and root vigor. The results indicated that seeds soaking with appropriate concentration of exogenous glycinebetaine could improve salt resistance of cucumber seedlings in a certain extent, and alleviate the inhibition of seedlings growth under salt stress. There was a certain different effect of exogenous glycinebetaine seed-soaking between neutral salt and alkaline salt stress on cucumber.
Keywords: plant ecophysiology; glycinebetaine; salt stress; cucumber; seedling; growth
 
Title: Effects of NaCl on seed germination of green and red-violet phenotype Suaeda salsa
Authors: LI Ke, YUE Xiaoxiang, CHEN Min, SONG Jie, WANG Baoshan
PP: 2014-2020
Abstract: Suaeda salsa is a succulent halophyte. S. salsa plants are green (green phenotype: G) when they grow in saline soil far from the intertidal area and are red-violet (red-violet phenotype: R) when they grow in intertidal area. In this paper, effects of NaCl treatment on seed germination of two S. salsa phenotypes were examined. The results showed that there were no significant differences in seed germination rate between two phenotypes compared to the control at low concentrations of NaCl (50, 100 mmol/L), and in the recovery percentage of red-violet phenotype S. salsa seeds and the green ones after the seeds which did not germinate at high concentrations of NaCl were transferred to distilled water for 4 days. However, the seed germination rates of both phenotypes were much lower at high concentrations of NaCl (400, 500 mmol/L)than those of the control. Red-violet phenotype seeds had higher germination potential and lower mean germination time than green ones. The contents of Na+ and K+ were not significantly different between two phenotype seeds and the seeds germinated in 200 mmol/L NaCl for 4 d. In contrast, the contents of soluble sugar and soluble protein of both red-violet phenotype seeds and the seeds germinated in 200 mmol/L NaCl for 4 d were significantly higher than those of the green ones. On the other hand, red-violet phenotype seeds were much heavier (per 1 000 grains) and bigger than green ones. These results suggest that the red-violet phenotype seeds from the plants which grow in intertidal saline soil are able to germinate under salt stress, hypoxia and low temperature via accumulating more organic substances, especially organic compatible solutes during seed maturation and germination.
Keywords: plant physiology; Suaeda salsa; phenotype; germination rate; recovery rate; NaCl
 
Title: Basic research on black soybeans drought resistance biology
Authors: YANG Weimin, YANG Liu, LI Tuanxia, ZHAO Qinghong
PP: 2007-2013
Abstract: With black soybean seedlings as a receptor, in the seedling growth period, processed the receptor with different concentrations of mannitol. Observed morphological characteristics and growth trend of black soybean seedlings, tested and analyzed their physiological and biochemical indicators. The results showed that under the water stress, a series of adaptive changes took place on the biological characteristics of black soybean seedlings, such as morphological characteristics, growth, physiological and biochemical indicators. As chemical mutagenesis substances, mannitol has significant osmotic adjustment effect for improving the drought resistance of black soybean seedlings.
Keywords: botany; drought resistance; black soybean; water stress; morphological characteristics; osmotic adjustment
 
Title: A primary study on the tricotyledon mutant of Dianthus plumarius
Authors: LIU Fang, LI Yuan
PP: 2000-2006
Abstract: A mutant with three cotyledons was occasionally observed during the study on Dianthus plumarius seeds germination. This mutant was transplanted and propagated by vegetative fashion. A comparative study on morphology of this mutant and wild type plants indicated that there were significant differences in stem type, leafy shape, cuticular waxy distribution and stomatic distribution of the mutant compared with that of wild type plants.
Keywords: plant developmental biology; Dianthus plumarius; tricotyledon mutant; cuticular wax; stomata
 
Title: Predict genomic islands in Bacillus cereus genome by using segment algorithm
Authors: NING Lvwen, GUO Fengbiao
PP: 1992-1999
Abstract: Genomic islands are large fragments obtained by horizontal transfer in bacterial genomes. Often, genes in genomic island are not essential to bacteria, but they may have indeed important biological functions. In this paper, the segmentation algorithm, combined with Z curve method, is used to predict genomic islands in nine sequenced Bacillus Cereus genomes. Through analysis, more than 50 genomic islands are found in the nine genomes. Based on the annotation information, the predicted islands all have some characteristics of transfer and most of them contain genes play an important role for the growth of bacteria or the resistance of bacteria. This work may contribute to understanding the morphology, phylogeny of Bacillus cereus, and the evolution of bacterial genomes.
Keywords: biophysics; genomic island; Z curve; segment algorithm; bacillus cereus
 
Title: Construction, expression and characterization of fusion proteins consisting of novel fluorescent protein and recombinant FLAG tag
Authors: YI Ke, GAO Xiaolian
PP: 1983-1991
Abstract: Based on FLAG-tag initial sequence (FLAG4: DYKDDDDK), designed four new FLAG tags and fused with H10 and G2. Western blotting was employed and the result showed that monoclonal antibody ANTI-FLAG M2 (AFM2) interacted specifically with five kinds of FP-FLAG fusion protein(G2-FLAG4, G2-FLAG5, H10-FLAG3, H10-FLAG4, H10-FLAG5).The functional affinity of FP-FLAG fusion protein and monoclonal antibody ANTI-FLAG M2 (AFM2) was determined by non-competitive ELISA. Results show that AFM2 against FLAG-tag initial sequence have the highest affinity, DYKDEDDK, DYKDYKDYK take the second place and DYKGGGYK is incapable of binding the antibody of AFM2. Using different affinity of these FLAG-tags, by designing different elution sequence and elution condition various FLAG fusion proteins can be gradually purified.
Keywords: peptide and protein biochemistry; recombination FLAG-tag; novel fluorescent proteins; non-competitive ELISA; affinity constant
 
Title: Optimization of synthesis method of N-Boc-Glu
Authors: YANG Mingfeng, JIANG Zhenju, ZHAO Ping, ZHAO Xuzhuang, WU Canjun
PP: 1978-1982
Abstract: The paper takes methanol as homogeneous reaction medium, Boc-anhydride and glutamic acid as material, and alkaling condition as reaction condition. The optimal synthesis condition was determined by measuring the melting point, the content of carboxylgroup and the product purity. The results show that the best experiment condition is as follows: The proportion of adding amount proportion of reactants (Glu∶Boc2O) is 1.0∶1.5, the temperature of the reaction is room temperature, the time of the reaction is about 23 hours, the pH value is 8-9, and the crystallization condition is the optimal synthesis condition of acidification after EtOAc is added to the solution.
Keywords: biochemistry; Boc-anhydride; glutamic acid; synthesis; optimization of method
 
Title: Discovery of a super-dwarf mutant of Arabidopsis thaliana and identification of it’s growth phenotype
Authors: QU Xiaoqing, GONG Yuanyong, LIU Laihua
PP: 1974-1977
Abstract: Most recently, our research group has stumbled across and identified a novel super-dwarf mutant (SDM) of Arabidopsis thaliana, which under normal growth conditions shows an abnormal developmental and growth phenotype with the characteristics of small and frizzled leaves, about 1% height of its wild type, five weeks longer life circle as well as less and smaller siliques compared to the wild type plant.
Keywords: molecular biology; Arabidopsis thaliana; dwarfism; super dwarf mutant; growth phenotype
 
Title: Sequence analysis and prokaryotic expression of a novel BmUFP gene from silkworm, Bombyx mori
Authors: HU Bo, ZHANG Yaozhou
PP: 1966-1973
Abstract: A novel gene sequence was obtained from silkworm pupa cDNA library constructed by our laboratory, and the GenBank accession number was BAD05929. After Blastn based on NCBI database and alignment of deduced amino acid sequence homology, it was found to be an unknown gene, and named as BmUFP. The full length of the gene was 774 bp. It contained an open reading frame (ORF) of 516 bp, 67 bp nucleotide sequence in 5′untranslated region (UTR) and 191 bp nucleotide sequence in 3′UTR, and no introns. The BmUFP gene encoded 171 amino acids, with their predicted mass 18.49 ku and isoelectric point 8.72, a basic protein. It was predicted that BmUFP contained a putative conserved domain of Reeler Superfamily. The ORF fragment of BmUFP gene and pET28a expression vector were ligated. The soluble BmUFP protein induced by IPTG in E. coli Rosetta (DE3) was expressed, which may lay a foundation for the study of BmUFP gene function.
Keywords: molecular biology; BmUFP protein; prokaryotic expression; silkworm; novel gene
 
Title: Functions of transcription factor ISL1 on cell proliferation
Authors: GUO Ting, WANG Weiping, ZHOU Chunyan
PP: 1961-1965
Abstract: ISL1 is a LIM-homeodomain transcription factor. Studies show that ISL1 plays important roles for cell proliferation. ISL1 is required for proliferation, migration and survival of cardiac progenitor cells. It also has important roles on pancreatic islets development, the proliferation and survival of the endocrine cells in pancreatic islets. ISL1 is indispensable for motor neural specialization. It promotes the proliferation and the repair of injured motor neurons. The overexpression of ISL1 may correlate with tumors’s development and may serve as a molecular marker for clinical diagnosis of some tumors. This review focuses on the research advances of ISL1 on cell proliferation during recent years.
Keywords: molecular biology; ISL1; reviews; proliferation; apoptosis; tumor
 
Title: Progress in resolution and synthesis of important chiral intermediate mandelic acid by enzyme
Authors: ZHANG Guoyan, CAO Shugui
PP: 1955-1960
Abstract: Enzymes are remarkable catalysts with wide substrate specificities, high stereoselectivity, high regioselectivity, mild reaction conditions and low pollution. Recent research has focused on exploring enzyme-catalyzed reaction to produce chiral intermediate mandelic acids. The study on the recent advances and future development of resolution and synthesis of chiral intermediate mandelic acid by enzyme has been discussed here.
Keywords: biochemistry and molecular biology; chiral intermediate; reviews; mandelic acid; biocatalyst; enzyme; synthesis; resolution