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December 15,2016
Volume 9,Issue 23
Pages -
Subject Area:Epidemiology,Forensic Medicine,Human Physiology,Biomedical Engineering

Title: Research on the effects of IL-17 on the contents of RORγ-t、IκBζ signal transduction factors in moxibustion regulation experimental RA rats

Authors: GAO Zishan, GAN Junxue, GUO Jiangyan, FU Shuping, GAN Ke
PP: 2479-2488
Abstract: Objective: To explore the interleukin17 (IL-17) signaling mechanism regarding to anti-inflammation effect of moxibustion in rheumatoid arthritis (RA) rats. Methods: 66 male SD rats were randomly assigned to control group, control+moxa group, RA model group (model), RA model moxa group (moxa), IL-17 knockdown+moxa group, IL-17 overexpression+moxa group, IL-17 knockdown group, IL-17 overexpression group. In the former 6 groups, each group has 9 SD rats, while 6 SD rats each in the latter two groups. 0.15 mL complete Freund’s adjuvant were injected into the right paw of SD rats for establishing RA model and using lentivirus injection to establish IL-17 knockdown/overexpression models. 18 days moxibustion treatments were conducted after 7 days injection. The treatment was that 5 times everyday moxibustion in each “Zusanli” and “Shenyu” points in SD rats. Changes of swelling degrees in RA rats’ right posterior foot before and after the model establishing, and then the moxibustion treatment were measured. Levels of IL-17, retinoid related orphan receptor gamma-t (RORγ-t) and recombinant inhibitory subunit of NF kappa B zeta (IκBζ) were measured using ELISA and Western blotting. Results: After 7 days injection, the swelling degree of RA rats’ right posterior foot was significantly higher than control group (P<0.01). After moxibustion treatment, swelling degree became significantly lower than model group (P<0.01). After IL-17 injection, IL-17 concentration were obviously higher in the IL-17 overexpression group than in the model group (P<0.05), while the concentrration of IL-17 got lower in IL-17 knockdown group than model group (P<0.05). After 18 days moxibustion treatment, comparing to model group, moxa group could significantly decrease the IL-17 and RORγ-t concentration that were increased by adjuvant arthritis (AA) rats after establishing complete Freund’s adjuvant model (P<0.05), while increase the IκBζ level (P<0.01). After moxibustion treatment, levels of IL-17 and RORγ-t in the serum of IL-17 overexpression+moxa group were increased than in moxa group (P<0.05); while these levels drop in the IL-17 knockdown+moxa group (P<0.01); however, there had shown no significant change in the IκBζ level among overexpression, knockdown and moxa groups. Conculsion: Through regulating IL-17 level, moxibustion treatment could have played a role in adjusting the RORγ-t and IκBζ concentration in RA rats’ ankle joint, thus demonstrated a cooperative anti-arthritis effect.
Keywords: acupuncture; moxibustion; rheumatoid arthritis; interleukin 17; RORγ-t; IκBζ; anti-inflammation

Title: Simultaneous determination of 16 components in Yinhuang granules by UPLC-QqQ-MS/MS

Authors: TAI Yanni, ZENG Fantian, ZHOU Xiaohong, XIANG Qing, XU Wen, HUANG Mingqing
PP: 2470-2478
Abstract: To use a rapid and accurate ultra-high performance liquid chromatography-tandem triple quadruple mass spectrometry (UPLC-QqQ-MS/MS) method for the determination of 16 main components (Neochlorogenic acid, Chlorogenic acid, Caffeic acid, Cryptochlorogenic acid, Rutin, Scutellarin, Cynaroside, Isochlorogenic acid B, Isochlorogenic acid A, Isochlorogenic acid C, Baicalin, Wogonoside, Luteolin, Baicalein, Chrysin, and Oroxylin A) in Yinhuang granules. The UPLC-QqQ-MS/MS assay was performed on a Waters CORTECS C18 (2.1 mm×100 mm, 1.6 μm) column with acetonitrile-water (containing 0.1% formic acid) as the mobile phase by gradient elution at a flow rate of 0.25 mL/min; The column temperature was set as 45℃. MS detection was performed with multiple reaction monitoring (MRM) mode using negative electrospray ionization. Satisfactory linearity was achieved within the investigated linear range and with fine determination coefficient (R>0.995 2), the overall recoveries were ranged from 98.67% to 100.76% with the relative standard deviation (RSD) ranging from 2.7% to 4.5%. It is the first report of simultaneous analysis of 16 major components in Yinhuang granules by using UPLC-QqQ-MS/MS method, which affords a highly sentive, specific, speedy and efficient method for controling the quality of Yinhuang granules.
Keywords: traditional Chinese medicine; Yinhuang granules; UPLC-QqQ-MS/MS; quantitative determination

Title: Effect of regulatory B cells on Th immune responses in fibrosis lung of silicosis mice

Authors: LIU Fangwei, LI Chao, CHEN Jie
PP: 2465-2469
Abstract: Objective: To observe the effect of regulatory B cells (Bregs) on Th immune responses in silica-induced lung fibrosis in mice. Methods: Healthy adult female C57BL/6 mice were divided into three groups: control group, silicosis group and Bregs depletion group. To set up the silicosis model, mice in silicosis group and Bregs depletion group received 50 μL of silica suspension (3 g/50 mL) intratracheally. To deplete Bregs, mice in Bregs depletion group were injected intraperitoneally with 300 μg anti-CD22 mAb one day before the silica exposure. Mice were sacrificed at 7, 28, 56 days after silica exposure. Broncho alveolar lavage fluid (BALF) and lung tissues were obtained. The hydroxyproline content in mice lungs was obtained and the secretions of cytokines interleukin-2 (IL-2) and IL-13 were examined by enzyme linked immunosorbent assay (ELISA). Results: The hydroxyproline content of lung in Bregs depletion group decreased obviously at day 56 after silica exposure compared with that in silicosis group (P<0.05). The secretion of IL-2 in Bregs depletion group increased significantly at day 28 after silica exposure compared with that in control group and silicosis group (P<0.05). The secretion of IL-13 in Bregs depletion group decreased significantly at day 56 after silica exposure compared with that in silicosis group (P<0.05). Conclusion: Bregs promoted the collagen deposition in mice lung by affecting Th1/Th2 immune balance.
Keywords: labour hygiene; public health; silicosis; regulatory B cells; Th responses

Title: Study of the impact of clinical pathway implementation on the quality of care for invasive breast cancer

Authors: BAO Han, SU Shaofei, WANG Xinyu, LIU Meina
PP: 2460-2464
Abstract: Objective: By comparing the concordance rates of quality indicators before and after the implementation of clinical pathway on invasive breast cancer, the study aimed to evaluate the effectiveness of clinical pathway on the quality of breast cancer care. Methods: The medical records of patients diagnosed as invasive breast cancer before and after the implementation of clinical pathway were extracted as the control group and the intervention group, respectively. Baseline characteristics between two groups were compared by using univariate analysis. Concordance rates of quality indicators before and after the implementation of clinical pathway were compared by using multivariate Logistic regression adjusting for relevant imbalanced baseline characteristics. Comparisons of the total and preoperative length of hospital stay between two groups were conducted by using Wilcoxon rank sum test. Results: A total of 578 invasive breast cancer patients were enrolled in this study with 283 in the control group and 295 in the intervention group. There were statistically significant differences in age, stage, histological grade and human epidermal growth factor receptor-2 (Her-2) status between the patients in two groups. After adjusting imbalanced baseline characteristics, we observed a statistically significant improvement in the concordance rates of indicators including fine-needle aspiration, sentinel lymph nodes biopsy (SLNB), and the proportion of patients who had their tumor hormone receptor status documented in their pathology report. The mean length of hospital stay was significantly reduced from 9 days to 8 days and the mean preoperative stay increased. Conclusion: The implementation of clinical pathway for breast cancer had bridged the gap between clinical practice and evidence-based therapy in areas like fine-needle aspiration, SLNB and tumor hormone receptor status documentation. It had a positive effect on the improvement of quality of care for invasive breast cancer.
Keywords: epidemiology; breast cancer; clinical pathway; quality of care

Title: Analysis of an abnormal peak at DYS391

Authors: LANG Min, YE Yi, HOU Yiping
PP: 2457-2459
Abstract: When applied the AGCU Database Y24 kit into the Chinese Han population data of sonthwest China, an abnormal peak at DYS391 was interestingly observed when using POP-7TM polymer on Y-chromosomal short tandem repeat (Y-STR) by ABI PRISM 3130 Genetic Analyzer, which may be a potential risk of wrong genotyping. Then, after we repeated the experiment using the POP-4TM polymer, the abnormal peak appeared far away from the typing zone. Here, we reported this phenomenon to remind that using AGCU Database Y24 shonld be careful when choosing the type of polymer.
Keywords: forensic medicine; abnormal peak; capillary electrophoresis; AGCU Database Y24; POP-7TM polymer

Title: Low-concentration of autoantibodies against angiotensin II type 1 receptor inhibit the aldosterone secretion of human adrenocortical cell lines

Authors: WANG Pengli, LIAO Yang, LEI Jinghui, WU Ye, ZHANG Suli, LIU Huirong
PP: 2450-2456
Abstract: Objective: To explore the effect and mechanism of autoantibodies against angiotensin II type 1 receptor (AT1-AA) on aldosterone (ALD) secretion of human adrenocortical cell lines (H295R). Methods: Hybridoma technology was used to prepare AT1-AA. Different concentration of AT1-AA and angiotensin II (AngII) were added into H295R cells for 6, 12, 24, 48, 72 h, ALD levels in the supernate were analyzed by radioimmunoassay. Western blotting was used to analyze the changes of the rate-limiting enzyme of ALD synthetase-CPY11B2, and the blocking effects of angiotensin II type 1 receptor (AT1R) specific blocker valsartan (VST) and synthetic second extracellular loop of angiotensin II type 1 receptor (AT1-ECII) antigen peptides were observed. Results: H295R cells treated with high concentration of AT1-AA in 10-6 mol/L and 10-7 mol/L for 72 h can increase the ALD secretion, whereas low concentration of AT1-AA in 10-8 mol/L and 10-9 mol/L can decrease the ALD secretion by H295R cells after 72 h incubation. After treatment with VST and AT1-ECII, the effect of AT1-AA-induced down-regulated ALD secretion can be blocked. Additionally, AT1-AA in 10-9 mol/L can inhibit the expression of CYP11B2 after 72 h. Conclusion: AT1-AA in high concentration and low concentration have opposite effects on ALD secretion, while low concentration of AT1-AA can inhibit the ALD secretion by H295R cells via AT1R, and this effect may be due to the inhibition of expression of ALD synthetase-CYP11B2 caused by AT1-AA.
Keywords: pathophysiology; aldosterone; angiotensin II type 1 receptor; autoantibody; CYP11B2; preeclampsia

Title: Protectiverole of GABA against ethanol hepatotoxicity involves inhibition of endoplasmic reticulum unfolded protein response

Authors: LIU Yanli, LUO Dan, LI Tao, WANG Shuanglian
PP: 2445-2449
Abstract: Using MTT and Western blotting analysis, whether unfolded protein response (UPR) of endoplasmic reticulum (ER) involves in the cytoprotective effect of gamma-aminobutyric acid (GABA) against ethanol-induced hepatotoxicity was investigated. The results showed that ethanol-induced decrease of cell viability was inhibited by GABA treatmentin cultured BRL hepatocytes. GABA inhibited ethanol-induced up-regulated expression of GRP78 and CHOP, which are UPR markers. These results suggest that inhibition of UPR is involved in the protective role of GABA against ethanol hepatotoxicity.
Keywords: human physiology; liver physiology; alcoholic liver disease; gamma-aminobutyric acid; unfolded protein response; proliferation

Title: Three novel single nucleotide variations in HLA associated with susceptibility to chronic hepatitis B

Authors: JIANG Danhua, LIAO Qijun, ZHAO Qiang, WANG Yiming
PP: 2440-2444
Abstract: Objective: Mining from our previous exome sequencing data of extreme phenotypic individuals, to unravel and identify single nucleotide variations (SNV) associated with susceptibility to chronic hepatitis B (CHB) in region of human leucocyte antigen (HLA) genes. Methods: We selected 99 CHB “susceptible” individuals and 90 CHB “resistance” individuals and performed exome sequencing on these individuals. We selected all coding region variations, SNV and small insertion and deletion (INDEL) in the HLA region from the exome sequencing data. We performed statistical analysis to select variations significantly different between the two groups. We further performed haplotype constructionand bioinformatics studies on the significant variations. Results: Three previously unreported novel variations, rs10093, rs12722039 and rs12722042 (rs10093: P=0.004 2, OR=2.164 6; rs12722039: P=0.001 6, OR=2.512 2; rs12722042: P=0.001 6, OR=2.512 2) in the HLA regions were associated with CHB. Our haplotype analysis showed that the three variations were located in the HLA-DQA1*0104. Bioinformatics analysis showed that the three variations damaged the functions of the wild-type alleles. Conclusion: We have discovered three new genetic variations, rs10093, rs12722039 and rs12722042, associated with susceptibility to CHB. This adds novel information on our understandings of HLA in the pathogenesis of CHB.
Keywords: medical genetics; chronic hepatitis B; exome sequencing; human leucocyte antigen; single nucleotide variations

Title: Role of TRPC1 in the cardiomyocyte hypertrophy induced by Visfatin

Authors: LI Junli, ZHAO Mingyue, WU Wenchao, LIU Xiaojing
PP: 2433-2439
Abstract: Objective: The aim of this study is to investigate the role and mechanism of transient receptor potential channels (TRPC1) in the cardiomyocyte hypertrophy induced by Visfatin by constructing the specific gene expression plasmid. Methods: Cultured H9c2 cardiomyocytes were exposed to Visfatin at different times in vitro, and the expression of cardiomyocyte hypertrophy markers and TRPC1 were detected. Moreover, constructed TRPC1-shRNA expressing plasmid was used to transfect the cells before Visfatin stimulation, and the overexpression plasmid of TRPC1 was performed to induce TRPC1 forced expression. Results: The experimental data demonstrated that exposure to 100 µg/L concentration of Visfatin for 24, 48, 72 h significantly increased the expression of marker genes for cardiomyocyte hypertrophy and TRPC1. Furthermore, transfection of specific TRPC1-shRNA for 48 h partially inhibited the Visfatin-induced mRNA expression of brain natriuretic peptide (BNP) in H9c2 cells, whereas overexpression of TRPC1 promoted the level of BNP. Conclusion: The results suggest that TRPC1 might mediate cardiomyocyte hypertrophy induced by Visfatin.
Keywords: medical cell biology; TRPC1; Visfatin; cardiomyocyte hypertrophy

Title: Experimental study of a membrane-based microfluidic approach for adding/removing cryoprotectant agents

Authors: YIN Laishan, ZHOU Xiaoming, WANG Zhikun, HUA Youwen, ZHANG Ying
PP: 2426-2432
Abstract: Along with the wide application of stem cells and other materials in clinical and scientific researches, cryopreservation faces a growing number of small volume objects. Some new requirements have been raised during adding/removing cryoprotectant agents (CPAs) for such small volume objects. In this study, a novel membrane-based microfluidic approach was presented. Accordingly, a membrane-based microfluidic system was designed and fabricated by microfluidics and membrane separation technique. Experiment results demonstrated that the system can achieve stable and controllable adding/removing CPAs for small volume samples, and it will greatly improve the efficiency and effectiveness of adding/removing CPAs for small volume biological samples.
Keywords: biomedical engineering; cryoprotectant agents; microfluidic chip; membrane separation technique

Title: The study of multifunctional carbon dots for microRNA real-time tracking and delivery

Authors: ZHANG Miaomiao, ZHAO Xuefen, FANG Zhengzou, NIU Yuanyuan, DU Fengyi, XIA Sheng, LOU Jiaming
PP: 2420-2425
Abstract: microRNA (miRNA) has great potential in preclinical medicine research and clinical treatment due to they can inhibit target genes expression by transcription level or protein level. However, there is lack of gene carriers which can be used in intracelluar tracking and gene transportation. Hyaluronic acid- polyethyleneimine functionalized carbon dots (HP-CDs) were prepared by one-step hydrothemal method. Transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), fluorescence spectrum, X-ray diffraction (XRD) spectra were used to characterize the physiochemical properties of HP-CDs. The results demonstrated that HP-CDs possessed uniform size distribution (～3.2 nm), a large number of hydrophilic groups, stable fluorescence and high quantum yeild (13.2%). Meanwhile, HP-CDs were endowed with favorable biocompatibility, gene loading and miRNA-30b transfection capacity. HP-CDs which go into cells can emit fluorescence, which make it a kind of effective tracking gene vector for visualization research of miRNA.
Keywords: biomedical engineering; nitrogen-doped carbon dots; hyaluronic acid; polyethylenimine; gene transportation

Title: UTMD combining with nanoparticles for glioma targeted therapy

Authors: YANG Jingjing, ZHAO Yingzheng, LU Cuitao
PP: 2411-2419
Abstract: Ultrasound-targeted microbubble destruction (UTMD) technology shows a lot of advantages in drug targeting delivery systems. UTMD technology combined with Cilengitide nanoparticles were used to improve the effect of gliomas targeted therapy. W/W type of Cilengitide-loaded gelatin-poloxamer nanoparticles were prepared by novel methods, and ultrasound-lyophilization method was used to prepare phospholipid-based microbubbles. The characterization of Cilengitide nanoparticles were evaluated by the appearance of the solution, particle size, Zeta potentials and drug encapsulation efficiency. C6 cells were used to evaluate the antitumor effects of Cilengitide nanoparticles combined with UTMD technology in vitro. Glioma models were established by using male SD rats. HE stain and immunohistochemistry were used to evaluate the anti-tumor effect in vivo. The results show that the concentration of phospholipid-based microbubble is 2×109/mL, with an average particle size of 3.26 μm. Cilengitide nanoparticles possess good elliptical morphology and excellent dispersion performance. The average particle size of Cilengitide nanoparticles is (112.00±1.63) nm. The Zeta potential value is -(15.8±0.2) mV. The encapsulation efficiency of Cilengitide nanoparticles reaches (87.5±1.4)%. Cytotoxicity test, HE staining and immunohistochemistry confirm UTMD technology combined with Cilengitide nanoparticles can obviously facilitate uptake of Cilengitide in glioma, and enhance the apoptosis of the tumor in vivo. In conclusion, UTMD technology combined with Cilengitide nanoparticles significantly improves the glioma-targeted therapeutic effect, showing great potentials in clinical application.
Keywords: pharmaceutics; gliomas; nano technology; ultrasound-targeted microbubble destruction; Cilengitide; targeted therapy

Title: Preparation and application of high performance liquid chromatography titanium gel monolithic column

Authors: DING Xiali, DONG Yuming
PP: 2403-2410
Abstract: Compared with silica-based monolithic column, titanium gel monolithic stationary phase has higher selectivity, specificity and pH stability, especially with regard to the separation and enrichment of biological samples of phosphate groups, and it has shown a good application value. Advances in preparation of titanium gel monolithic columns and their applications in high performance liquid chromatography (HPLC) are reviewed in this paper in order to draw attention and development prospect in the field of their manufacturing process.
Keywords: pharmaceutical analysis; high performance liquid chromatography; review; titanium gel monolithic column; preparation method; application progress

Title: Influence of nursing intervention for patients with curative effect and complications after procedure of prolapse for hemorrhoids of circumferential mixed hemorrhoids

Authors: ZENG Huiting
PP: 2396-2402
Abstract: Objective: To explore the influence of nursing intervention for patients with curative effect and complications after procedure of prolapse for hemorrhoids (PPH) of circumferential mixed hemorrhoids, and to provide guidance for clinical nursing in the future. Methods: From February 2014 to December 2015, a total of 60 cases of circumferential mixed hemorrhoids inpatients in our hospital were collected, and they were divided randomly into two groups, control group and nursing intervention group. Each group had 30 cases. They were treated with procedure of prolapse for hemorrhoids. The control group adopted the traditional nursing method, while the nursing intervention group was given the whole course high quality nursing service on the basis of the traditional nursing method. The postoperative efficacy, recurrence rate, complications and patient satisfaction percentage with nursing work between two groups were observed and compared. Results: The postoperative efficacy of nursing intervention group was better than control group (96.7% vs 83.4%, P<0.05); recurrence rate and complications in nursing intervention group were significantly lower than control group (P<0.05); the satisfaction rate on nursing job in nursing intervention group was higher than control group (96.7% vs 80.1%, P<0.05). Conclusion: Application of nursing intervention for patients with circumferential mixed hemorrhoids after PPH operation can obviously improve the curative effect and satisfaction, and significantly reduce the postoperative reecurrence rate and complications, so it is worthy of clinical application.
Keywords: nursing; nursing intervention; procedure of prolapse for hemorrhoids; curative effect; complications

Title: Research progress on the effect and mechanism of ERK1/2 signaling pathway in cerebral ischemia reperfusion

Authors: GUO Kaihang, WANG Jialin, XING Jia, HE Lijuan, ZHU Xiaochen, GUO Rongjuan
PP: 2388-2395
Abstract: The mechanism and signal transduction pathway of brain tissue injury caused by ischemic cerebrovascular disease have become a hot spot of research for several years. As a classic and important signaling pathway, current studies show that ERK1/2 signaling pathway participates in the process of neuronal cell apoptosis after cerebral ischemic injury, but the specific effects and mechanisms are still controversial. In this paper, we present comprehensively the research achievements and literatures about the correlation between cerebral ischemic injury and ERK1/2 signaling pathway, and give some advice for the future study.
Keywords: neurology; cerebral ischemia reperfusion; review; ERK1/2 signaling pathway

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