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ISSN 1674-2850
CN 11-9150/N5
 
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October 15,2009
Volume 2,Issue 19
Pages 1987-2094
Subject Area:Ecology,Animal Auxology,Animal Reproductive Biology,Applied Microbiology,Fermentation Engineering,Genetic Engineering
 
Title: Research on creative “platforms+modules” training mode of PE teachers
Authors: ZHU Ailing, WANG Qiaojun
PP: 2089~2094
Abstract: Based on knowledge of the innovative talents training, this paper takes the creative “platforms+modules” training of physical education (PE) teachers of Ningbo University for example, makes a research on the training objective, specification, course structure and so on, and points out main characteristics and weaknesses of the training mode. Finally, this paper also makes some suggestions to solve the problems and further improves the training mode. The paper provides some references for further reform of PE professional training in China.
Keywords: sport pedagogics; physical education teachers; training mode; “platforms+modules”; innovative talents
 
Title: SWOT analysis and routing of China’s professional sports industry against the background of economic globalization
Authors: LI Qin, LI Wenhui, LIU Hongjian
PP: 2083~2088
Abstract: It is considered that there are advantages and disadvantages of China’s professional sports industry, and economic globalization has brought opportunities and challenges for it through the strength, weakness, opportunity and threat (SWOT) analysis on China’s professional sports industry against the background of economic globalization. In order to get a healthy development, the professional sports industry should create a good system environment from system, organization and industry aspects and so on, improve administrative organizations, train professionals, and provide good professional sports products.
Keywords: sport economics; economic globalization; professional sports industry; strength, weakness, opportunity and threat analysis; routing
 
Title: Analysis on light equipment aerobics game of the 8th National Game of College Students
Authors: LI Xianxiong, YANG Fang
PP: 2076~2082
Abstract: This paper analyzes several aspects on the hand apparatus six-person calisthenics of the 8th National Game of College Students, such as selection and using of apparatus and the art arrangement of sets of aerobics movements, by the method of document-data, video-recording analysis, mathematical statistics and comparative analysis, etc. The results show there are a lot of problems in the selection and using of apparatus and the art arrangement of light apparatus six-person calisthenics of every delegation. Aiming at solving these problems, the paper puts forward some corresponding countermeasures, so as to provide some valuable references for the future development of the light apparatus calisthenics in China.
Keywords: athletic training; aerobics; light equipment; game; analysis
 
Title: Effect of different digestive solutions on PK-15 cells
Authors: ZHENG Liyong, MA Fenglong, LIU Pingping
PP: 2072~2075
Abstract: This paper aims at searching for a suitable digestion method for PK-15 cells. PK-15 cells was digested by 0.25%, 0.15%, 0.10%, and 0.05% trypsin, and 0.05% trypsin+0.53 mmol/L EDTA Na solution. The PK-15 cell proliferation and passage effects were observed, so as to determine the different digestion effects of the PK-15 cells in the digestive solutions. The results showed that the 0.05% trypsin+0.53 mmol/L EDTA Na solution had the best digestion effect on the PK-15 cells, and the growth of the cells after the digestion is better.
Keywords: cell biology in other disciplines; PK-15 cells; cell culture; trypsin; cell digestion
 
Title: Comparative study on purification efficiency among three dominant aquatic plant species in constructed wetlands of Chengdu living-water park
Authors: YANG Xiaocheng, KANG Zihua
PP: 2065~2071
Abstract: Some water samples from intakes and outlets of 3 wetland pond beds growing Phragmites communis Trin, Typha angustifolia and Cyperus alternifolius were respectively taken for monitoring and contrasting 5 main water quality indexes including dissolved oxygen (DO), chemical oxygen demand (CODcr), biochemical oxygen demand in 5 days (BOD5), total nitrogen (TN), and total phosphorus (TP) month by month all year around, so as to discover influences of different plant species on water purification efficiency. The paper has briefly discussed the possible reason why the 3 aquatic plant species have respective different wastewater purification efficiencies. It is shown that the three plant species all have evident purification effects to the Jinjiang river flowing to the wetlands, but the removal rates of the pollutants are significantly different according to seasons and the plant growing conditions. In general, the wastewater treatment efficiency of Phragmites communis Trin wetland is the best and Typha angustifolia wetland is the second among the 3 plant species, whereas, Cyperus alternifolius is better to remove overmuch phosphorus than the other 2 aquatic plant species.
Keywords: ecology; constructed wetlands; wastewater treat efficiency; aquatic plant; Chengdu living-water park
 
Title: Preliminary study on relative fatness in Eothenomys miletus in Hengduan mountains
Authors: ZHU Wanlong, WANG Rui, XIAO Caihong, LIU Pengfei, JIA Ting, CAI Jinhong, LIAN Xiao, WANG Zhengkun
PP: 2058~2064
Abstract: Relative fatness was measured in Eothenomys miletus in hengduan mountains. In order to study the relationship between body status and circumstances in E. miletus, the relative fatness in E. miletus was calculate by empirical formula (K=100 W/L3). The results indicated that there were no significant sexual differences with respect to relative fatness; the relative fatness showed no significant differences in the groups with different ages; but the relative fatness showed significant differences in different seasons. Seasonally trends were lower in winter and lowest in spring; then highest in summer and higher in autumn. The changes pattern of E. miletus in different seasons were related to their microenvironment such as low latitude, high elevation, and lower average ambient temperature in Hengduan mountains.
Keywords: zoology; relative fatness; Eothenomys miletus; Hengduan mountains
 
Title: Effects of impulsive perturbations on a food chain model with distributed time delay
Authors: GUO Zhongkai, LI Zizhen,, MA Zhihui, WANG Wenting
PP: 2051~2057
Abstract: In this paper, a three-species food chain system with impulsive perturbations and distributed time delay is investigated. Firstly, by using Floquet theory of impulsive differential equation and comparison theorem, the global stability of periodic predator eradication solution was considered. Secondly, the conditions which guarantee the three species’ permanences were obtained. Finally, the effects of top predator release quantity on the three-species food chain system were analyzed.
Keywords: mathematical ecology; impulsive perturbation; distributed time delay; comparison theorem; Floquet theory
 
Title: Study on foraging patterns of Messor aciculatus(F. Smith)and its impact on seed densities and plant distribution in a desert steppe ecosystem
Authors: XIN Ming,ZHANG Dazhi,HE Dahan
PP: 2044~2050
Abstract: Messor aciculatus(F. Smith) is a dominant seed harvester ant in the desert steppe ecosystem of northwest China. The results from the house raising experiments and the field investigations showed that 27 kinds of plant seeds could be foraged by the ant. Among the seeds, those with sides of 1.20~2.50 mm and with edges and corners and easy to carry are most collected. In natural conditions the kinds of collected seeds and their composition in the nest depend on the number and distribution of the plants nearby the nest. The collection and storage by the ant influence germination capacity of the seeds, especially those annual seeds with light weight. The ant foraging directly affects the seed density in soil seed bank, and the degree is different according to different plant kinds, and the annual herb plants are most influenced. It is indirect effect mainly on the composition and spatial pattern of distribution for plant community.
Keywords: entomology; Messor aciculatus; foraging pattern; seed bank; plant distribution; desert steppe ecosystem
 
Title: Carabus species in Nanling National Nature Reserve of Guangdong (Insecta: Coleoptera: Carabidae)
Authors: CHEN Kaixuan, GAO Lei, GONG Yuening, XIE Guoguang, TIAN Mingyi
PP: 2039~2043
Abstract: In this paper, the genus Carabus (Insecta: Coleoptera: Carabidae) and its distribution characteristics in Nanling National Nature Reserve of Guangdong were briefly introduced. In total, 4 subgenera and 10 species (subspecies) of Carabus have been collected from the reserve: C. (Apotomopterus) sauteri nanlingensis Deuve et Tian, C. (A.) shun floridus Cavazzuti et Ratti, C. (A.) shikengkong Deuve et Tian, C. (A.) novemunus Deuve, C. (A.) acorep costulomicans Deuve et Tian, C. (A.) arrowi yubeicus Deuve et Tian, C. (Isiocarabus) hunanicola guangdongicola Deuve et Tian, C. (I.) hunanicola klapperichioides Deuve, C. (Archaeocarabus) gressittianus bambousi cola Deuve et Tian, and C. (Damaster) ignimitella angulicollis Hauser.
Keywords: insect taxonomy; Insecta; Coleoptera; Carabidae; Carabus; Nanling National Nature Reserve
 
Title: Inheritance of selenium contents and QTL detection in rice grains
Authors: ZHENG Jiakui, ZHANG Xianwei, YANG Li, ZHANG Tao
PP: 2033~2038
Abstract: Selenium (Se) has been recognized as an essential trace element for human beings and plays a number of functions important to human health. Understanding the genetic of Se contents in rice and quantitative trait loci (QTL) mapping are the basis for effective selection in Se breeding program. In the present study, a set of 145 F2 lines derived from the cross between Fengxin red rice and Minghui 100 were used to detect QTLs associated with Se contents with 92 SSR markers covering the whole rice genome. A total of 6 QTLs were detected for Se contents on chromosome 2, 3, 5, 6, 7, respectively. Accounting for general contributions range of 4.10%~12.74% to Se contents. qSe3 was located between rm7 and rm251 on chromosome3 with the largest general contributions 12.74% to phenotypic variation. The favorable allele of qSe3 for Se contents originated from Fengxin red rice and was controlled by dominant effect. And the total general contributions of 6 QTLs was 42.19% with cumulative effect. This helps to further understand the genetic basis for Se contents and will provide the basis for gene mapping of Se contents and molecular marker-assisted selection of Se in rice.
Keywords: genetics; quantitative trait loci; genetic analysis; selenium content; rice
 
Title: In vitro culture of wild red deer (Cervus elaphus)-bovine heterogenerous embryos generated by cloning
Authors: LIU Tao, PENG Xinrong, QIAN Qijun
PP: 2028~2032
Abstract: Red deer fibroblast cells were fused into enucleated bovine oocytes, and red deer-bovine heterogenerous embryos were reconstructed by cloning. The experiment results showed that the reconstructed heterogenerous embryos could develop into blastocyst stage with the blastocyst rate 15.1%. Bovine homogenerous cloning embryos were reconstructed as the control group. The cleavage rate of the heterogenerous embryos were not significantly different with the control group, however, the blastocyst rate were significantly (P<0.05) lower than that of the control group(15.1% vs 22.5%), and the Hochest 33342 immunostaining showed that the cell number of the heterogenerous embryos were significantly (P<0.05)lower than that of the control group (68.4 vs 83.8).
Keywords: developmental biology; heterogenerous embryos; cloning; red deer; blastocyst rate; blastocyst cell number
 
Title: Prokaryotic expression, purification of recombinant Kazal-type peptidase inhibitor and sperm gelatinase, Macrobrachium rosenbergii
Authors: QIAN Yeqing, LI Ye, YU Yanqin, YANG Weijun
PP: 2022~2027
Abstract: In previous reports, full-length cDNAs of a male reproduction-related peptidase inhibitor Kazal-type (MRPINK) and a sperm gelatinase (MSG) have been identified from the prawn, Macrobrachium rosenbergii. The expression vector pET-28a(+)-MRPINK and pET-28a(+)-MSG were constructed by inserting MRPINK and MSG cDNA into 6譎is tag pET-28a(+), respectively. Through digestion with restriction enzymes and sequence analysis, the right expression strain were selected after transformation of recombined plasmid into E. coli BL21 (DE3) and the fusion proteins with His-tag were efficiently expressed after IPTG induction. SDS-PAGE analysis revealed that the molecular weight of rMRPINK and rMSG were 14.7 and 15.7 ku with the expected size, respectively, and both of them were expressed in inclusion bodies. Using Ni-NTA purification system, these recombinant proteins were efficiently purified under denature conditions and purified protein can be used in the further study of inhibitor and enzyme interaction mode and physiological functions.
Keywords: biology of reproduction; Macrobrachium rosenbergii; male reproduction-related peptidase inhibitor; Kazal-type peptidase inhibitor; sperm gelatinase; prokaryotic expression; purification
 
Title: Preliminary research on in vitro evaluation of antiviral drug by phage-indicator model
Authors: YANG Zhiyong, LI Tiemin, ZHANG Rui, ZHANG Bo, HU Yongfei, LI Yu
PP: 2018~2021
Abstract: This paper develops a novel method which evaluates antiviral drug in vitro by phage-indicator model. The phage inactivation experiments were carried out with antiviral drugs Shuang Huanglian (SH), Ribavirin and Monoammonium Glycyrrizinate (MG). Firstly the toxicity of 3 drugs to indicator (E. coli) was determined to get rid of interference of the drugs in the model system. The results indicated that SH and Ribavirin had toxicity to the indicator, which was relative to their doses. The drug act of 3 drugs was detected in the model. The data demonstrated that the phage survival was significantly inhibited by SH and Ribavirin. After the interaction of the drugs and phage for 20 h, the high inhibition rate of SH and Ribavirin to phage survival reached to 89.69% and 47.50% respectively, while MG had no inhibition to phage. On the base of these experimental results, evaluation of antiviral drugs in vitro by means of the phage-indicator model is feasible and practicable, which is suitable for screening of antiviral drugs, especially for those isolated from a large number of natural products.
Keywords: microorganism application; drug screening; phage-indicator model; antivirus drug
 
Title: Phylogenic analysis of rhizobia associated with Vigna unguiculata and Vigna radiata in subtropical region of China
Authors: ZHANG Yongfa, HAN Lili, YANG Jianying, TIAN Changfu, CHEN Wenfeng, CHEN Wenxin
PP: 2008~2017
Abstract: Objective: To investigate the phylogenic status of cowpea (Vigna unguiculata) and mung bean (V. radiata) grown in subtropical region of China. Methods: A polyphasic approach, including numerical taxonomy, amplified 16 S rDNA restriction analysis(ARDRA) and phylogenic analysis of 16 S rRNA sequences, was used to 62 experimental strains from different provinces of subtropical region in China. Results: 90% of the analyzed strains belonged to or related to Bradyrhizobium japonicum, B. liaoningense, B. yuanmingense and B. elkanii, while the remaining were Rhizobium leguminosarum, R. etli and Sinorhizobium fredii. Conclusion: Abundant diversity was observed in cowpea and mung bean grown in the subtropical region of China.
Keywords: microbiology; phylogeny; Rhizobia; cowpea; mung bean; subtropics
 
Title: Response of tomato microRNA and their target mRNAs to virus infection
Authors: LIU Xin, FENG Junli, WANG Kai, CHEN Jishuang
PP: 1999~2007
Abstract: MicroRNA (miRNA) plays an important role in miRNA gene silencing pathway, which contributes a lot in plant developmental system. This experiment chose three plant viruses from different strains (CMV-Fny, CMV-FnyΔ2b, TAV-Bj) and inoculated them to tomato seedlings. At different periods of infection, the expressing levels of seven miRNAs and partial mRNAs were quantified in four different kinds of tissues by stem-loop real-time reverse transcription polymerase chain reaction (real-time RT-PCR). The analysis studied the change and influence of miRNA gene regulatory pathway in plant bodies during the interaction between plant virus and their hosts. The exhibited phenomena and laws have significant relationship with the damage to the host defense mechanism caused by virus, time-space specificity expression of the miRNA and the target mRNA, biological regulation function of the specific miRNA, and so on.
Keywords: microbiology; microRNA; real-time reverse transcription polymerase chain reaction; tomato; cucumber mosaic virus; tomato aspermy virus
 
Title: Study on the mutant breeding of Corynebacterium glutamicum SYPS-062, direct-producing L-Serine from sugar
Authors: XU Guoqiang, ZHANG Xiaomei, DOU Wenfang, XU Hongyu, XU Zhenghong
PP: 1994~1998
Abstract: Corynebacterium glutamicum SYPS-062, which could use sugar to produce L-Ser, was used as an original strain for stepwise mutagenic treatment by diethyl sulfate (DES). Then positive mutants were selected on D-Ser resistance gradient plate. Finally a strain named 33a-18 was obtained. The 33a-18 can accumulate 11.0 g/L of L-Ser, which is increased by 65.4% compared with the original strain.
Keywords: fermentation engineering; L-Serine; mutagenesis; Corynebacterium glutamicum
 
Title: Research on application of reverse vaccinology in protective antigen screening
Authors: SONG Zhenwei, ZHOU Xuezhang, WANG Yujiong
PP: 1987~1993
Abstract: The rapidly-developed reverse vaccinology was a new strategy on the modern study of vaccinology, which marked a turning point. Based on the principle of “sequence-structure-function”, the strategy taked immunization information science、computer prediction design and high-throughput of the various groups (including genomics, transcription of this group, proteomics and so on) as its core. It was hoped that the strategy can give new lights to find more protective antigen of pathogen as the candidates to produce vaccine, and improve our efficiency to vaccine screening.
Keywords: genetic engineering; reverse vaccinology; review; screening protective antigen