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ISSN 1674-2850
CN 11-9150/N5
 
Current Issue
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June 15,2009
Volume 2,Issue 11
Pages 1117-1214
Subject Area:Animal Breeding,Animal Anatomy, Animal Physiology,Endemiology,Geriatrics,Cancer Therapeutics,General Surgery,Cancer Diagnosis
 
Title: Study on genetic transcription difference of liver tissues of nascent black-bone chicken, layer and broiler
Authors: ZHAO Xiaoling, LI Liang, XIAO Lian, ZHU Qing
PP: 1208~1214
Abstract: Objective: To study expression and regulation mechanisms of proteins related with specific characteristics in Silky fowl and Sichuan mountain black-bone chicken. Methods: Differentially display reverse transcription polymerase chain reaction (PCR) and gene chip scanning were adopted to attain expressed sequence tag (EST) which were differentially displayed among the black-bone chicken, the layer and the broiler. Results: Three ESTs were obtained. Bioinformation analysis proved that DQ003271 only expressed in the layer was a new EST, located at the fifth chromosome; and DQ003272 only expressed in the black-bone chicken was a known EST, belonging to Gga.9136. The first sequence of positive reading frame of contig 1 was 91% homologous to NP_001012582.1. The gene including the segment may directly control the expression of Tyrosinase, thereby controlling the black color trait. DQ003274 only not expressed in the Sichuan mountain black-bone chicken had 100% consistency with CD737238.1 and belonged to Gga.8993. Conclusion: These results provide significant data for further research on different traits among the black-bone chicken, the layer and the broiler.
Keywords: animal genetics and breeding; mRNA differential display polymerase chain reaction; black-bone chicken; expressed sequence tags; gene chip
 
Title: Expression and location characteristics of chicken invariant chain P31 gene and its point mutant in eukaryotic cells
Authors: LI Lin, ZHONG Dalian, YU Weiyi
PP: 1203~1207
Abstract: To research the molecular immunoregulation function of chicken invariant chain (ChIi) and its mechanism, ChIi P31 gene was amplified from PMD18-T/Ii P31 via PCR using two pairs of primers, and the P31 gene was inserted into pEGFP-N1 and pEGFP-C1 respectively to form two recombinant plasmids, N1-P31 and C1-P31. 4 mutation primers were designed, and two point mutations were applied using large primer mutation method to construct C1-P8-18A plasmids. After double enzyme digestion and sequencing identification, the three plasmids were respectively transfected into human 293 cell strain, and the cells were cultivated for 48 h and then observed by a fluorescent microscope. The results showed that fluorescence of the cells transfected by N1-P31 and C1-P31 mainly appeared in organelles in the cytoplasmic domain, while the green fluorescence of the cells transaffected by the C1-P8-18A and pEGFP mainly distributed in the nucleuses. All these indicate that the ChIi molecules in fusion proteins ChIi-GFP and GFP-ChIi have biological function of transportation in eukaryotic cells, and the ChIi molecules have endosome location function.
Keywords: veterinary immunology; immune gene; chicken invariant chain; green fluorescent protein; eukaryotic expression
 
Title: Construction of transient expression vector containing hsp23.7 promoter of Bombyx mori and its activity detection
Authors: ZHANG Xing, HU Xiaolong, WANG Xiaojuan, XIE Min, YU Xiaohua, XUE Renyu, CAO Guangli, GONG Chengliang
PP: 1194~1202
Abstract: In order to verify the activity of the heat shock protein gene promoter (hsp23.7) from silkworm, the hsp23.7 promoter was amplified and cloned into the vector pSK with red fluorescent protein gene (DsRed), then transient expression vector pSK-hsp23.7-DsRed-PolyA was constructed. It was transfected into silkworm BmN cells and silkworm tissues. The result showed that after the hsp23.7 promoter was treated using heat shock, it could induce DsRed gene expression in BmN cells and silkworm tissues. Consequently, the cloned hsp23.7 promoter sequence had the heat shock protein promoter activity. The sequence analysis indicated that secondary structure of hsp23.7 promoter sequence formed a complicated stem-loop structure which could be related with the function of the promoter.
Keywords: molecular biology; Bombyx mori; promoter of heat shock protein gene; activity detection
 
Title: The CT anatomy of nasal cavities and sinuses in Bactrian camel
Authors: WANG Hongju, BAI Zhongtian, YU Shiyuan, WANG Jianlin
PP: 1189~1193
Abstract: Objective: To study anatomical characteristics of nasal cavities and sinuses of Bactrian camel (Camelus bactrianus) and explore physiological adaptability of the Bactrian camel to dry and aeolian sandy environment. Methods: The Bactrian camel head was grossly dissected and continuously tomoscaned by 64-sliced spiral CT scanner for anatomical analysis. Results: The CT images accurately reflected the fine structures inside the nasal cavities and sinuses, and it was discovered that in the Bactrian camel the turbinates were large and complex, the corresponding meatus were narrow, and the sinuses were large. Conclusion: The nasal cavities and sinuses of the Bactrian camel are relatively complex, which helps the Bactrian camel to adapt the arid environment.
Keywords: anatomy; Bactrian camel; spiral CT; nasal cavity; nasal sinuses
 
Title: Application of heart rate variability in evaluation of dynamic workload
Authors: WANG Zhenglun, SONG Tingbo, WU Lei, XU Lei,LING Ruijie, YANG Lei
PP: 1185~1188
Abstract: Objective: To investigate whether heart rate variability (HRV) can be used to evaluate dynamic work1oad. Methods: 46 volunteers went up and down on a two-ladder platform (58 cm×35 cm×23 cm) at different speeds to simulate dynamic workload. ECG signals were recorded by Holter 8800 during the performance and transferred into digital signals. Total power (TP), low frequency (LF), high frequency (HF) and the ratio of HF to LF were analyzed by Holter software. At the same time, pulmonary ventilation volume, heart rate and values of Borg scale were record and analyzed. Results: 1) the pulmonary ventilation volume, the heart rate and the values of Borg scales at the three speeds all had significant differences (P<0.05), and all increased while the speed increased, showing a significant dose-response relationship. 2) All the values of HF and LF in the three dynamic exercises were lower than those in rest time (P<0.05). While the speed increased, the values of LF increased, however, the values of HF decreased. All the values of TP and the ratio of HF to LF in the three dynamic exercises were higher than those in rest time. While the speeds increased, the values also increased. Conclusion: The dynamic workload can be evaluated by Borg scale and heart rate variability as well as conventional indexes including pulmonary ventilation volume and heart rate.
Keywords: labor hygiene; dynamic work; heart rate variability; pulmonary ventilation volume; Borg scale
 
Title: Influence of subchronic sodium arsenite poisoning on antioxidation level of mouse liver and brain tissues
Authors: WU Shunhua, YAO Qin, ZHENG Yujian, TANG Hongying, WU Jun
PP: 1180~1184
Abstract: Objective: To investigate the influence of subchronic sodium arsenite(NaAsO2) exposure on lipid peroxidation and antioxidation in mouse liver and brain tissues. Methods: Subchronic toxicity experiment was applied. 60 Kunming mice were randomly divided into four groups according to their body weights, including control group (C), low dose arsenic poisoning group (L), middle dose arsenic poisoning group (M), and high dose arsenic poisoning group (H). Mice of the control group were fed with distilled water. Mice in group L, group M and group H were respectively fed with water with dissolved sodium arsenite at concentrations of 0.6 mg/L, 2.5 mg/L and 10.0mg/L. The experiment lasted for 90 d. Then the content of lipid peroxidation product malondialdehyde (MDA) and activities of the total superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione S-transferase (GST) in the mouse liver and brain tissues were determined respectively. Results: With the increase of the sodium arsenite dose, the MDA content significantly increased (P<0.05), and the activities of SOD and GSH-Px significantly decreased (P<0.05); and with the increase of the sodium arsenite dose, the GST activity increased firstly and decreased subsequently (P<0.05). Conclusion: The subchronic sodium arsenite poisoning can increase lipid peroxidation and decrease antioxidation in mouse liver and brain, and the oxidation/antioxidation level variation has tissue specificity. It indicates that lipid peroxidation may be one of the mechanisms of liver and brain injury in arsenic poisoning.
Keywords: occupational and environmental health; sodium arsenite; liver and brain; oxidation/antioxidation
 
Title: Evaluation on potential values of ethno-medicines in the treatment of hepatitis
Authors: PU Yuanzhu, TONG Yingpeng, WANG Zhang, FAN Gang, ZHANG Yi
PP: 1174~1179
Abstract: 108 plant medicines from 52 families usually employed by 27 ethnic minorities for hepatitis treatment were evaluated with scoring and ranking method. The results showed that medicines including Bidens bipinnata L., Centella asiatica (L.) Urban, Phyllanthus emblica L., and Swertia bimaculata (Sieb.et Zucc.) Hook.f. et Thoms.ex C.B. Clarke had good application prospects in the treatment of hepatitis. The results have some reference significance for further research and development of these ethno-medicines for treating hepatitis.
Keywords: ethno-medicine; hepatitis; scoring and ranking method; potential value; evaluation
 
Title: 海洋真菌Aspergillus aculeatus次级代谢产物的研究
Authors: WANG Yu, LI Zhanlin, LIU Tao, TIAN Li, HUA Huiming
PP: 1170~1173
Abstract: In order to search new antitumor metabolites from secondary metabolites of marine microorganisms and provide lead compounds for developing new antitumor medicines, the secondary metabolites of marine fungus Aspergillus aculeatus, which was obtained from mangrove plant Acanthus ebracteatus in the South China Sea, were investigated. Eight compounds were isolated and identified from acetone ultrasonic extract of mycelia and ethyl acetate extract of fermentation broth of the Aspergillus aculeatus. The eight compounds comprised 1,3,5-trihydroxy-7-methylanthraquinone (1); 2-hydroxy-3-(4-hydroxy-phenyl)-propionic acid (2); 3-isopropyl-6-methylpiperazine-2, 5-dione (3); 3-sec-butyl-6-methylpiperazine-2, 5-dione (4); uridine (5); uracil (6); adenine (7); and succinic acid (8).
Keywords: medicinal chemistry; marine fungus; Aspergillus aculeatus; secondary metabolite
 
Title: Study on purification processes of total flavone in Scutellaria baicalensis Georgi
Authors: WEI Yuanfeng, LIU Xinguo, HAN Jianwei, ZHANG Qiuxiang
PP: 1167~1169
Abstract: Objective: To select a preferable purification method of total flavone in Scutellaria baicalensis Georgi by studying the purification processes. Methods: The UV and HPLC were used as the analysis methods, and the contents and transfer rates of the total flavone, baicalin and wogonin were taken as the investigation indexes; the total flavone was purified by the precipitation separation with chlorhydric acid (PSCA) and HP20 macroporous adsorption resin; and the two methods were compared. Results: When the total flavone were purified by the PSCA, its degree of purity was 97.61%, the contents of the baicalin and wogonin were respectively, 9.19 g/100 g crude herb and 0.71 g/100 g crude herb, and the transfer rates were respectively 99.33%, 90.05%, and 82.96%; and when the total flavone were purified by the HP20 macroporous adsorption resin, its degree of purity was 80.66%, the contents of the baicalin and wogonin were respectively 8.97 g/100 g crude herb and 0.53 g/100 g crude herb, and the transfer rates were respectively 84.01%, 78.21% and 77.24%. Conclusion: The contents and transfer rates of the total flavone, baicalin, and wogonin dramatically increased when the PSCA was used, so the PSCA is the preferable technical process for the purification of the total flavone.
Keywords: Chinese pharmacy; total flavone in Scutellaria baicalensis Georgi; precipitation separation with chlorhydric acid; HP20 macroporous adsorption resin; purification
 
Title: Content determination of gallic acid in Duoxuekang by HPLC
Authors: ZHENG Haijie, GENG Zhipeng, FENG Guanrong, WANG Shu, ZHANG Yi, LAI Xianrong, SUOLANGQIMEI
PP: 1163~1166
Abstract: Objective: To establish a content determination method of gallic acid in Duoxuekang. Methods: The content of gallic acid in Duoxuekang was determined by high performance liquid chromatography (HPLC). Results: The gallic acid concentration had good linear relation with peak area integral quantity in the range of 9.6~240 mg/L, the average recovery rate was 99.50%, and relative standard deviation(RSD) was 1.38% (n=6). Conclusion: The method(HPLC) has high accuracy, reliability and reproducibility, so it can be used in the quality control of the preparation.
Keywords: Ethnic medicine; Duoxuekang; gallic acid; high performance liquid chromatography; content determination
 
Title: Metabonomic study on the action mechanism of metformin in the therapy of type Ⅱ diabetes mellitus
Authors: HUO Taoguang, CAI Shuang, LU Xiumei, XIONG Zhili, LI Famei
PP: 1157~1162
Abstract: Objective: To study the metabolic differences between type Ⅱ diabetes mellitus patients and patients treated with metformin and elucidate the action mechanism of metformin from the angle of metabonomic. Methods: Ultra-performance liquid chromatography tandem mass spectrometry (UPLC/MS/MS) was applied to the serum metabolite profiling in type Ⅱ diabetes mellitus (DM-Ⅱ) patients and patients treated with metformin. Principal components analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) models were employed for the discrimination of the two groups. Results: Significant difference in endogenous metabolite profiles was observed in DM-Ⅱ patients and metformin treated patients. Five potential biomarkers were discovered. Possible action mechanism of metformin was discussed. Conclusion: Metformin can regulate the metabolic disorders in DM-Ⅱ patients, which may be associated with its effect to lower serum lysophosphatidylcholines.
Keywords: analytical chemistry; biomarkers; UPLC/MS/MS; metformin; type Ⅱ diabetes mellitus
 
Title: A study of the relationship between complements (C5a and CRP) and pulse wave velocity in patients with metabolic syndrome
Authors: FANG Yan, LIU Yongming, WANG Xiaoyu
PP: 1153~1156
Abstract: Objective: To investigate the relationship between complements (C5a and CRP) and pulse wave velocity (PWV) in patients with metabolic syndrome (MS). Methods: Twenty-six patients with MS were recruited in this study and thirty-four persons without MS served as a contrast group. Serum C5a, serum CRP, urine C5a, and PWV were examined, and the relationships among them were analyzed. Results: Compared with those of the contrast group, the urine C5a and cf-PWV of the MS patient group were significantly higher, but the two groups had no statistical difference in the serum C5a, serum CRP, cr-PWV and cd-PWV; the cf-PWV was positively correlated with age, systolic pressure and the urine C5a, but was not correlated with the serum CRP and C5a; and the cd-PWV was positively correlated with the urine C5a and age. Conclusion: The MS patient group has increased PWV and urine C5a concentration, and the PWV is correlated with the urine C5a.
Keywords: internal medicine; metabolic syndrome; atherosclerosis; complement; pulse wave velocity
 
Title: Generation of recombinant adenovirus carrying tumor specific expression E1a gene
Authors: LIU Shanling, XIAO Xue, CHEN Xinlian, WANG He
PP: 1147~1152
Abstract: Objective: Replication-deficient adenovirus, one of the most commonly used vectors in cancer gene therapy, has its advantage of safety when carrying target therapeutic gene into tumor cells. On the other hand, usually it can not efficiently deliver the gene into most of the tumor cells due to lack of replication ability. Telomerase activity is detected in immortal cell lines and an extraordinary diverse set of tumor tissue, but is not detected in most of normal tissues. Thus’ it is regarded as an ideal target for treating proliferation related disease, such as cancer. This research is to generate adenovirus which can specifically replicate in tumor cells. Methods: Telomerase promoter and adenovirus early transcript gene E1a were cloned and constructed into a shuttle plasmid in which E1a expression was controlled by telomerase promoter. The recombination of the shuttle plasmid and viral backbone plasmid was performed in bacteria, and adenovirus was generated in packaging cell line 293. Results: An adenovirus encoding E1a gene under the control of telomerase promoter was generated using AdEasy system. Conclusion: The establishing method of tumor specific expression of E1a could be used in further study of cancer gene therapy strategy regarding tumor specific replication of adenovirus carrying target gene.
Keywords: oncology; recombinant adenovirus; telomerase promoter; E1a gene; gene therapy
 
Title: Comparison on the passage methods of the Vx2 metastatic liver cancer animal models
Authors: LIU Yuhao, GE Haiyan, LI Shuping, KE Bo, CHANG Zhuolin, SHEN Rongrong
PP: 1142~1146
Abstract: Objective: To investigate the difference in rabbits’ survival time and transplanted tumor quality between two transplanted groups. One group is the rabbit Vx2 transplantation tumor transplanted into rabbits’ muscle of posterior limb for going down to the future generation, the other is into rabbits’ liver. As known the rabbit Vx2 transplantation tumor’s growth rate is high. Methods: Employ tumor transplants to set up the muscle tumor model and the liver cancer model from rabbit Vx2 tumor. Apply magnetic resonance to detect the tumorous growth. Biopsy the tumor tissues which grow in rabbits’ muscle of posterior limb or in liver regularly, in order to get the message about tumorous growth. Record and compare the experimental rabbits’ tumorous growth and the rabbits’ survival time. Results: Set up stable rabbit Vx2 posterior limb’s muscle tumor model and liver tumor model successfully by tumor transplants, within the 25 rabbits’ muscle of posterior limb being transplanted, about 20 had tumor growth ultimately, the other 25 rabbits were transplanted into liver, about 22 had tumor growth ultimately, the tumor formation rate of the two groups was alike (P>0.05). Comparison of tumors’ volume at the 7th day and the 14th day after transplantation between the posterior limb group and the liver group showed that the latter exceeded the former significantly (P<0.01). The tumor’s TGR of the former group was 64.9% at the 10th day after transplantation, the latter group was 94.6%; then the former was 70.1% and the latter was 96.7% at the 20th day after transplantation. About 3/20 rabbits with tumor in the muscle of posterior limb and 20/22 in the liver could survive for 30 days(P<0.05). Conclusion: Contrast to the muscle tumor group, the tumor volume of liver cancer group is larger, and its growing period is longer, the tumor parenchymal cells are richer, and cellular necrosis degree is smaller, which indicates that it is a relatively ideal passage model.
Keywords: oncology; Vx2 transplantation tumor; passage; model
 
Title: Correlation analysis of expression of BRCA1 protein and related factors in sporadic breast cancer
Authors: REN Jie, WEI Minjie, MA Wenfeng, WANG Lin, JIN Feng, MI Xiaoyi, JIN Wanbao
PP: 1137~1141
Abstract: This paper selected 121 sporadic breast cancer and 20 benign breast diseases, the expression level of BRCA1, ER and HER-2 protein was detected by immunohistochemistry (IHC). The factors affect the expression of BRCA1, and the correlation between expression of BRCA1 and the clinical parameters were analyzed. In the benign breast tissues, the positive expression of BRCA1 and ER protein are found in the nuclei. While in the breast cancer tissues, there are low or loss expression of BRCA1 and ER protein, the differences are significant (P<0.05). There is negative correlation between the expression of BRCA1 and over expression of HER-2. The expression of BRCA1 in breast cancer is negatively correlated with the age of patient. The expression of BRCA1 protein is negatively correlated with the metastasis of axillary lymph nodes. The expression of BRCA1 with axillary lymph hode metastasis decreases more significantly. There is no significant correlation between the BRCA1 protein expression and the pathology types of the breast cancer. The detection of BRCA1 expression combining with the expression of ER and HER-2, together with the clinical parameters, is beneficial to the diagnosis and prognosis of sporadic breast carcinoma.
Keywords: molecular oncology; sporadic breast cancer; BRCA1; ER; HER-2
 
Title: Association of nonsyndromic cleft lip with or without cleft palate with transforming growth factor β3 gene polymorphism
Authors: LIN Jianyan, GUO Zeqiang, CHEN Yongjuan, DING Kaihong, LUAN Rongsheng
PP: 1131~1136
Abstract: Objective: To explore the relationship between the 641C/A and 15572G/-polymorphisms in transforming growth factor-β3(TGFβ3) gene and the risk of nonsyndromic cleft lip with or without cleft palate (nsCL/P) in Chinese population. Methods: Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) technique was used to detect the TGFβ3 641C/A and 15572G/-genotypes in 212 patients with nsCL/P and 221 controls. And the specificity of this technique was confirmed by direct sequencing of the PCR products. Results: The genotype and allele frequencies of TGFβ3 641C/A and 15572G/-had no significant difference between nsCL/P and controls (P>0.05). All haplotypes also had no significant difference between two groups in haplotype analysis (P>0.05). Conclusion: There is no association between TGFβ3 641C/A and 15572G/-polymorphisms with the development of nsCL/P in the Chinese population.
Keywords: stomatology; nonsyndromic cleft lip with or without cleft palate; transforming growth factor β3; gene polymorphism; genetic susceptibility
 
Title: Notch3 gene mutations in two CADASIL kindreds with migraine
Authors: YIN Xinzhen, LIU Jianren, ZHANG Baorong, ZHANG Liang, WANG Peizhen, ZHOU Fuyou, DING Meiping, ZHAO Guohua
PP: 1125~1130
Abstract: Objective: To investigate the clinical features of two kindreds with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) and analyze Notch3 gene mutations. Methods: Five patients of the family members were studied through clinical examinations. Notch3 gene mutations in four patients, four unaffected family members and 120 unrelated normal controls were detected via DNA sequence analysis method. Results: The patients in these families showed typical migraine, stroke and memory decline; and cranial magnetic resonance imaging (MRI) revealed subcortical white matter infarct and leukoencephalopathy. A 397C→T (R133C) mutation and a 268C→T (R90C) mutation were identified in these two pedigrees respectively, and these two mutations were co-segregated with the diseases. The 397C→T mutation has not been reported previously in China. Conclusion: These two CADASIL is due to Notch3 mutations, and the patients with CADASIL in these two pedigrees show migraine.
Keywords: neurology; cerebral artery diseases; migraine; Notch3 gene; mutation
 
Title: Expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in rat lung tissue after cigarette-smoking exposure
Authors: WU Jie, CHEN Ping, XIE Lihua, CAI Shan, CHEN Jianbo, ZHANG Cheng, LIU Shaokun
PP: 1117~1124
Abstract: Objective: To study the expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) in the lung tissue of rat with chronic obstructive pulmonary disease (COPD) and vascular endothelial cell after cigarette-smoking exposure. Methods: Twenty-four male SD rats were randomly divided into a normal control (N) group and a COPD model (M) group. The rat model of COPD was established by exposure to cigarette smoking. Rats’ general condition, weight changes, lung function, pathologic features of lung tissues and inflammatory cell differentials in bronchoalveolar lavage fluid (BALF) and expression intensity of MMP-9 and TIMP-1 in lung tissue were investigated. The vascular endothelial cell was stimulated by different concentrations of cigarette smok extract (CSE) and Western blot was carried out to examine the expression of MMP-9 and TIMP-1. Results: There were some changes such as falling off of hair, dullness and breathing in M group’s rats while the rats of group N had no evident change. There were significant differences in the body weight, lung function index, inflammatory cell count, lung histomorphologic index and expressions of MMP-9 and TIPM-1 between groups N and M. The expressions of MMP-9 and TIMP-1 in the vascular endothelial cells increased with the concentration of CSE. Conclusion: Smoking is one of key etiological factors for COPD. The imbalance of MMP-9/TIMP-1 in lung tissue and the proteolytic enzyme produced by vascular endothelial cell may play an important role in the pathogenesis of COPD.
Keywords: respiratory diseases; vascular endothelial cell; pulmonary disease; chronic obstructive; metalloproteinase