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ISSN 1674-2850
CN 11-9150/N5
 
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October 15,2008
Volume 1,Issue 11
Pages 1213-1314
Subject Area:Developmental Neurobiology,Ecology,Biochemistry,Fermentation Engineering,Bionics
 
Title: The secondary structure of winter-wheat-bran antifreeze protein determined by FT-IR and CD spectrum
Authors: ZHANG Chao, ZHANG Hui, ZHAO Xiaoyan, MA Yue, YAO Huiyuan
PP: 1266~1271
Abstract: The secondary structure of the winter-wheat-bran antifreeze protein was analyzed by the fourier transform infrared (FT-IR) and circular dichroism (CD) spectrum. The smooth peak of the amide I and amide III region from the FT-IR profile was overlapped by the characteristic peak. So the characteristic peak of the α-helix, β-sheet, β-turn and random coil of winter-wheat-bran antifreeze protein was resolved. The content of the α-helix, β-sheet, β-turn and random coil was the accumulation of the characteristic peak respectively. Moreover, the secondary structure was evaluated by the CD spectrum. Finally, the difference between the FT-IR and CD spectrum results was discussed.
Keywords: analytical chemistry; winter-wheat-bran antifreeze protein; secondary structure; fourier transform infrared spectrum; circular dichroism spectrum; amide Ⅰ and Ⅲ
 
Title: The Psychometric analysis of the resilience scale for adults
Authors: YANG Lizhuang, LV Yanzhou
PP: 1310~1314
Abstract: This paper translated, revised and pre-tested the resilience scale for adults (RSA), and analyzed the psychometric properties about RSA. A sample of 390 college students was tested. The descriptive statistic analysis revealed that the total score of RSA was in line with normal distribution, but the 33 items were heavily skewed. Cronbach index α was used as an indicator of reliability. The overall reliability was 0.87, and the reliability of factors was about 0.7 except the factor of social ability is 0.54. The result of the confirmatory factor analysis showed that the six-factor model can fit the data very well. The fit index of RMSEA was 0.47, NNFI was 0.94, CFI was 0.95.
Keywords: psychometrics; resilience scale for adults; reliability; validity; confirmatory factor analysis
 
Title: Research on the influence of Special Olympic Games on physical and mental health of the mentally retarded adults
Authors: ZHANG Jiancheng, WU Xueping
PP: 1305~1309
Abstract: The mission of Special Olympics is to provide year-round sports training and athletic competition in a variety of Olympic-type sports for 170 million children and adults with intellectual disabilities, giving them continuing opportunities to develop physical fitness and improving the lives of them. But the research about how to give them some help about Special Olympics and the influence of Special Olympic Games on physical and mental health of the mentally retarded is poverty in our country now. In experimental method, this paper focuses on the influence of Special Olympic Games on the physical and mental health of the mentally retarded adults with low and medium level. The research demonstrates that with a certain time and an amount of exercises, the mentally retarded adults’ physical and mental health can better promote. It is good for us to further explore the appropriate way for adults with intellectual disabilities to participate in Special Olympics movement, and to get the aim of letting them integration into the society gradually.
Keywords: special pedagogy; mentally retarded adults; Special Olympic Games; physical and mental health
 
Title: The establishment of genetic transformation system in cerebral cortex of mouse embryo via in utero electroporation
Authors: YU Huali, JU Xingda, WANG Junjie, GAO Qianqian, FU Xiuqing, WANG Xingzhi, ZHU Xiaojuan
PP: 1300~1304
Abstract: Recently, as a new and effective gene transfer method,electroporation has been applied to the study of developmental biology, especially to central nervous system development. In this study, in utero transfection plasmid DNA method was set up to embryonic mouse. The GFP expression vector pCAGGS-IRES-EGFP was microinjected into the lateral cerebral ventricle of E 14.5d mouse embryos through the uterine wall. Then, the 30 V square-wave pulse was delivered across the head for 5 times and interval 50m sec by electrodes in parallel with the median raphes. Embryos were then allowed to develop for three days. The slices of transfected brain were checked under a fluorescence dissection microscope. The results showed that GFP was expressed in the lateral cerebral cortex. This system is of great significance to the further studies of cerebral cortex development, neuronal migration, axonal guidance and gain-of-function and loss-of-function induction.
Keywords: developmental neurobiology; in utero electroporation; microinjection; cerebral cortex; mouse embryo
 
Title: Cladistics and phylogeny of genus (subgenus) relationships within Lebertiidae
Authors: GUO Jianjun, JIN Daochao
PP: 1297~1299
Abstract: This paper studied the phylogenetic relationships of 3 genera and 8 subgenera of Lebertiidae, using the software Hennig86 (V1.5). The cladistic analyses were made using 16 morphological characters. A classification scheme, based on the phylogenetic relationships within Lebertiidae, is proposed for 2 subfamilies: Septlebertiinae and Lebertiinae, and there is only one genus Septlebertia within Septlebertiinae, and 7 genera within Lebertiinae: Scutolebertia, Estellosus, Lebertia, Pilolebertia, Pseudolebertia, Hexalebertia and Mixolebertia.
Keywords: Zoology; phylogeny; cladistics; genus (subgenus); Lebertiidae
 
Title: Determination on skeleton and blood biochemistrical parameters of Bambusicola thoracica
Authors: WANG Dingjiang, YAO Yinhua, YANG Hanyuan, YU Zhibiao, WU Chaobin, WU Zhitao, CHENG Meirong, AI Xiaojie
PP: 1292~1296
Abstract: Twenty-four Bambusicola thoracica were used for skeleton system anatomy study and determination of blood biochemistrical parameters relating to protein and fat metabolism. The results showed that all the body skeleton parameters(except the No.2 metacarpus)of Bambusicola thoracica were significantly different (P<0.05). Also, the levels of total protein, albumin and globulin in female serum were higher than those of male, and glutamic-pyruvic transaminase was the same tendency too(P>0.05), whereas albumin/globulin in female serum was lower than that in male. The contents of total bilirubin, total cholesterol and triglycerides in female serum were 8.33%, 23.08%and 15.38% higher than those in male respectively, but the difference was not significant(P>0.05). The results indicated that the protein and lipoid metabolism were different in sexual distinction. It supplies the information for anatomy, metabolism and other studies in Bambusicola thoracica.
Keywords: zoology; Bambusicola thoracica; skeleton; blood; biochemistrical parameters
 
Title: Study on the classification and ordination of wetland vegetations in Lianbo flooding plain of Fen River in Shanxi Province
Authors: WANG Yan, ZHANG Feng
PP: 1285~1291
Abstract: The quantitative classification and ordination of wetland vegetations in Lianbo flooding plain of Fen River, Hejin, Shanxi were studied based on the 41 samples using two-way indicator species analysis (TWINSPAN) and detrended correspondence analysis (DCA). The 41 samples were classified into 7 associations, which are Ass. Myriophyllum verticillatum, Ass. Leymus secalinus, Ass. (Salix matsudana-Leymus secalinus+Typha minima), Ass. Imperata cylindrical, Ass. Inula japonica, Ass. Phragmites australis and Ass. (Typha angustifolia L.+Phragmites australis), by TWINSPAN, and the community characteristics of each association were described. The result of the ordination for 41 samples by DCA revealed the relationships between the pattern of these communities and certain environmental factors, especially the moisture in soil. The result of the ordination for 28 species DCA reflected the habitat characteristics and the distribution of the dominant species determined the distribution of the communities in a large measure.
Keywords: ecology; two-way indicator species analysis(TWINSPAN); detrended correspondence analysis(DCA); Lianbo flooding plain of Fen River; wetland vegetation
 
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Title: Principles and applications of magnetic techniques for the isolation and purification of proteins
Authors: YANG Keke, YANG Wanshen, WANG Xianxiang, WU Wenbing, WANG Sen
PP: 1272~1276
Abstract: Isolation and purification of proteins is required in almost all areas of biosciences and biotechnology. There are diverse procedures that can be used to achieve this goal up to now, but most of them are complicated and expensive. Recent successful syntheses of monodispersed magnetic nanoparticles has made the magnetic separation an emerging technology which can purify biological active compounds directly from crude samples. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The paper summarizes various methodologies, strategies and materials used in magnetic separation for target proteins.
Keywords: biochemistry; protein purification; review; principles and applications; magnetic isolation; immobilization
 
Title: Study on signal transduction pathway of Heregulin-α in mammary gland of mouse
Authors: GENG lijing, LI Qingzhang
PP: 1260~1265
Abstract: To determine the signal transduction pathway of Heregulin-α(HRG-α) in mammary gland, the mammary gland of mouse at 9 days in pregnancy, 12 days in lactation and 6 days in involution were cultured in vitro. Each individual mammary gland was cultured in the media Waymouth’s MB752/1 with or without HRG-α. Following 24 hours of culture, each mammary gland was digested to obtain epithelial cells, which were lysed and the lysate was used for Western blot analysis. The phosphory lation of PKC was detected by ELISA. Western blot results showed that HRG-α enhanced the phosphorylation of p44/42 MAPK, p38 MAPK and PKC in pregnancy; and, HRG-α promoted the phosphorylation of STAT5 and p38 MAPK, while it inhibited PKC phosphorylation in lactation; in involution, HRG-α induced STAT3 phosphorylation and inhibited PKC phosphorylation.
Keywords: biochemistry and molecular biology; signal transduction; Western blot; Heregulin-α; mouse; mammary gland
 
Title: Concentration profiles of microorganisms and their metabolites in a cylindrical pore during water flooding process
Authors: GANG Hongze, LIU Meitang, MOU Bozhong
PP: 1253~1259
Abstract: The paper develops a mathematical model to simulate the transport of microorganism and their metabolite in a finite cylindrical pore with carbon source adhered to its inside surface. The model considers parameters as microbial growth, metabolite formation, chemotaxis, diffusion and water flooding, and focused on the transport mechanism of microorganism and metabolite, especially the influence of water flooding velocity on the concentration profiles of microorganism and metabolite. Model prediction shows that concentrations of microorganism and metabolite at the water-oil interface are higher than that in the pore center region; within a certain region of water flooding velocity, concentrations of microorganism and metabolite can reach to a set of equilibrium concentrations; there exists a critical water flooding velocity at which the maximum equilibrium concentration of microorganism and the minimum equilibrium concentration of metabolite at the oil-water interface are developed; microorganisms and metabolites will be rushed out of the cylindrical pore when the water flooding velocity exceed this critical velocity. Prediction obtained here may be helpful to understand the mechanism of microbial enhanced oil recovery.
Keywords: applied microbiology; transport; modeling; pore; water flooding
 
Title: Screening of lipase-producing strain and optimization of fermentation conditions
Authors: ZHOU Haixia, YUAN Lihong, OUYANG Pingkai
PP: 1246~1252
Abstract: A wild strain of lipase-producing bacteria was isolated from the soil pretreated with Jatropha curcas seed powder by using Jatropha oil as sole carbon source and identified as Pseudomonas sp.. The fermentation conditions of the lipase-production strain were optimized by studying effects of carbon sources, nitrogen sources, and fermentation technics. The results showed that the optimal medium composition for lipase producing was as follows(%,v/v or w/v ): olive oil 2.0, yeast extract 0.5, (NH4)2SO4 0.5, MgCl2·6H2O 0.5. The optimal initial pH of fermentation medium was 6.5. The maximum yield of 14.17 U/mL was obtained when Pseudomonas sp. was shake-cultivated at 30℃ for 36 h with 180 r/min.
Keywords: fermentation engineering; lipase; Pseudomonas sp.; screening; fermentation conditions
 
Title: Chemical analysis of chemical composition of Chinese Tamarisk
Authors: LIU Song,LIAO Rongsu,LI Junqing
PP: 1241~1245
Abstract: The pre-experiment on plant chemical constituent from Chinese Tamarisk was established. Varietal identification and extraction and isolation on phenolic compounds were studied. The emphases were placed on sugar, glycosides, tannins, phenolic compounds, flavonoids, saponins, aminoacids, proteins, alkaloids, coumarin and anthraquinone. The result showed that sugar, glycosides, tannins and flavonoids were found in Chinese Tamarisk, and the sugar included reducing sugar, the glycosides included saponins. On the other hand, there were not found to be aminoacids, proteins, alkaloids, coumarin and anthraquinone in Chinese Tamarisk. According to the results, saponins and phenolic compounds are crude antioxygens and they can influence permeable membrane and the absorption of mineral ions(specially K+ and Ca2+). The result of extraction and isolation analysis further proved that plant chemical constituent are propitious to the growth of the plants.
Keywords: chemical ecology; Chinese Tamarisk; plant chemical constituent; qualitative analysis
 
Title: Acquisition of LOS5/ABA3 transgenic Kentucky bluegrass(Poa pratensis L.) and characteristics under drought and cold stress
Authors: SONG Zhe, ZHANG Jiachang, CHU Qingquan, LI Zhaohu
PP: 1233~1240
Abstract: Agrobacterium-mediated transformation was used on callus induced from the mature ‘merit’ Kentucky bluegrass seeds as explants. Callus containing LOS5/ABA3 gene were transferred to the improved MS-medium containing 100 mg/L Hygromycin B and callus that carried exposed to Hygromycin B, were regenerate as plants. The LOS5/ABA3 gene were transmitted to 35 plants which were confirmed by PCR analysis. Physiological analysis showed that the ABA content in LOS5/ABA3 transgenic plants were higher than in wild-type plants, both under normal and drought stress conditions. The rate of water lose from Kentucky bluegrass removed from the plant was lower in LOS5/ABA3 transgenic plants than in wild-type plants. Relative conductances under drought stress were lower in LOS5/ABA3 transgenic plants than in the wild-type plants. The percentage of leaves that faded under drought stress in drought stress examination, the LOS5/ABA3 transgenic plant’ the percentage of faded leaves in all leaves is lower then in the wild type plants, which proved that LOS5/ABA3 transgenic plants enhanced ability to tolerate drought stress. Under cold stress, wild-type plants had some leaves fading and their growth rate decreased, while LOS5/ABA3 transgenic plants grew normally, which proved that LOS5/ABA3 transgenic plants have greater ability to tolerate cold stress compare wild-type plants.
Keywords: molecular genetics; drought stress; Agrobacterium; Kentucky bluegrass; low temperature stress; LOS5
 
Title: Study on culturing features of 5 ectomycorrhizal fungi
Authors: YANG Yan, TANG Ming, ZHANG Ruqin, LI Cui
PP: 1228~1232
Abstract: The effects of different temperature, pH, carbon sources and nitrogen sources on the growth of five ectomycorrhizal fungi [Suillus bovinus (L. ex Fr.) Ktze., Suillus tomentosus (Kauff.) Sing. Snell & Dick, Xerocomus versicolor (Rostk.) Quél., Suillus lactifluus (With ex Gray) Sm. et Th. and Suillus luteus (L.ex Fr.)Gray] were tested. The results indicated that: 1) Five ectomycorrhizal fungi could not grow at 5℃, and the optimum growing temperature was 30℃, 30℃~35℃, 20℃~25℃, 20℃ for S. luteus and S. lactifluus, S. bovinus, S. tomentosus and X. versicolor, S. bovinus and S. luteus adapted to higher-temperature, because they could grow at 40℃; 2) All the ectomycorrhizal fungi were able to grow 1within the range of pH3.0~pH9.0, with the optimum pH value being pH7.0 for S. bovinus, X. versicolor and S. luteus, pH5.0 for S. lactifluus, and S. tomentosus had the same growth between pH5.0~pH7.0; 3) The optimum growing carbon sources for S. bovines, S. luteus and X. versicolor was glucose, as well as glucose and maltose had the same influence on the growth of S. lactifluus. The growth of S. tomentosus was not significantly different(P>0.05) on the 5 carbon sources; 4) The optimum growing nitrogen sources for S. bovines, S. lactifluus and S. luteus was malt extraction, together with peptone and malt extraction was the best for S. tomentosus, and ammonium dihydrogen phosphate, peptone and malt extraction had the same influence on the growth of X. versicolor.
Keywords: microbiology; culturing features; pure culture; ectomycorrhizal fungi
 
Title: Micro/ultra-structure and anisotropism of the rough surface on butterfly wing scale
Authors: FANG Yan, SUN Gang, CONG Qian, WAN Yanling
PP: 1223~1227
Abstract: 11 genus 14 species butterfly, which are common in northeast China, were collected to observe and determine the micro/ultra-structure on the rough surface of wing scale by means of scanning electron microscopy. Two methods, first-incline-then-drip method (FITD method) and first-drip-then-incline method (FDTI method), were adopted to measure the sliding angle (SA) of water droplet on wing surface by an optical contact angle measuring system. Based on the microstructure of scale, there are three sorts of scales, including angustifoliate shape, latifoliate shape, and round-leaved shape. Based on the ultrastructure of scale, there are five sorts of scales, including arch bridge shape, chessboard shape, single array sieve pore shape, double array sieve pore shape, and multi array sieve pore shape. The scales arrange like overlapping roof tiles. Anisotropism of the micro/ultra-structure of wing scale can be seen on both submicro-scale and nano-scale. The anisotropism of self-cleaning on wing surface can also be showed by the water droplet sliding angle values determined by the above two methods. The crucial role of scale in determining the self-cleaning effect on butterfly wing surface is proved by the water SAs on butterfly wing surface without scale.
Keywords: bionics; anisotropism; sessile drop; butterfly; rough surface; ultrastructure
 
Title: Cloning and expression of glucose dehydrogenase gene gdh in E.coli from Bacillus subtilis
Authors: ZHANG Liang, GONG Liangjie, DING Zhongyang, SHI Guiyang
PP: 1218~1222
Abstract: In this paper, primers were designed according to the encoding sequence of Bacillus subtilis glucose dehydrogenase and gdh gene was amplified by polymerase chain reaction (PCR) using the primers. Then the PCR product was ligated to plasmid pUC19 and the ligation product was transformed into Escherichia coli JM109. Positive recombinants were obtained through screening by Ampr. The sequence was compared with original sequence in the GenBank, and the alignment result showed 97% homology. Subsequently, the gdh gene was sub-cloned into expression vector pET-28a and transformed into Escherichia coli DE3 codon plus. The gdh gene was expressed successfully in E.coli and the activity of GDH in the cell-free extract of E.coli reached 0.003 254 U/mg.
Keywords: microbiology; Bacillus subtilis; glucose dehydrogenase; expression
 
Title: The molecular methods for identification of hydrophytic microbes
Authors: HU Xuelian, ZHANG Jun, SHAN Baoqing
PP: 1213~1217
Abstract: The classification and identification of the microorganisms is an important way to the study of microorganism. Traditionally, it is tedious and time-consuming because it follows the character of bioecology .With the development of molecular biology, the way of microbic identification becomes more precise. Most of the molecular biology methods are involved in polymerase chain reaction (PCR), for instants, temperature gradient gel electrophoresis, denaturing gradient gel electrophoresis, random sequencing in clone libraries, single-strand conformation polymorphism, terminal-restriction fragment length polymorphism, and so on. Some methods which are not involved in PCR are also be used widely, such as fluorescence in-situ hybridization, DNA re-association analysis. On the whole, we can get different information from different methods. This paper introduces some useful methods and shows their advantages and disadvantages.
Keywords: microbiology; classification and identification; review; molecular biological methods; polymerase chain reaction