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ISSN 1674-2850
CN 11-9150/N5
 
Current Issue
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June 15,2016
Volume 9,Issue 11
Pages -
Subject Area:Chemistry of Traditional Chinese Medicine,Children and Adolescents Hygiene,Health Administration
 
Title: Treatment of combination of acupuncture with medicine on constipation of Parkinson’s disease
Authors: LUO Wei, REN Jigang, LI Dehua, LIU Xuguang
PP: 1097-1100
Abstract: Constipation is one of the commonly encountered symptoms of autonomic nervous function in patients with Parkinson's disease (PD), and yet there is no effective drug treatment. According to traditional Chinese medicine (TCM) syndrome differentiation, the comprehensive therapy by acupuncture combined with Chinese medicine on constipation of PD has definite curative effect, which can significantly improve the PD patients with constipation, motor function and systemic symptoms. Combining with the cases, this paper discusses the etiopathogenisis and pathogenesis in TCM treatment on constipation of PD to provide more ideas and suggestions for the treatment of chronic disease with complex pathogenesis in TCM such as constipation of PD.
Keywords: science of acupuncture and moxibustion; Parkinson’s disease; constipation; combination of acupuncture and medicine; syndrome differentiation
 
Title: Chemical constituents of Chuanxiong Rhizoma
Authors: FANG Xin, MA Qiang, FENG Yi, LIANG Shuang
PP: 1090-1096
Abstract: Objective: To isolate and identify the chemical constituents of Chuanxiong Rhizoma. Methods: Compounds were isolated by various kinds of column chromatographies on silica gel, Sephadex LH-20, middle performance liquid chromatography (MPLC) and high performance liquid chromatography (HPLC), and their structures were elucidated by the physicochemical properties and spectral analysis including nuclear magnetic resonance (NMR) and mass spectrometry (MS). Results: Nineteen chemical constituents were obtained and identified as 3a-hydroxy-6-carbonyl-neocnidilide (1), senkyunolide A (2), ligustilide (3), Z-butylidenephthalide (4), butylphthalide (5), senkyunolide I (6), senkyunolide H (7), senkyunolide J (8), senkyunolide N (9), 3\',6\',8\',3a-diligustilide (10), Chuanxiongnolide A (11), levistolide A (12), Z-3\',8\',3\'a-7\'a-tetrahydro-6,3\',7\',7\'a-diligustilide-8\'-one (13), tokinolide B (14), exo-Z,Z \'-3a.7\'a,7.3\'a- diligustilide (15), Z-6,8\',7,3\'-diligustilide (16), 5-(hydroxymethyl)-2-furaldehyde (17), methyl caffeate (18), fumalic acid (19). Conclusion: Among the isolated 19 compounds, there are 9 phthalides (1-9), 7 dimerphthalides (10-16), and compound (1) is a new phthalide.
Keywords: chemistry of Chinese medicine; Chuanxiong Rhizoma; chemical constituents; phthalide; dimerphthalide
 
Title: Influence of cadmium exposure during pregnancy on offspings’ growth and learning and memorizing functions
Authors: ZHAO Qihong, LIU Qifei, HU Anla, LI Li, TAO Fangbiao
PP: 1174-1178
Abstract: Objective: The purpose of this study is to elucidate the influence of cadmium exposure during pregnancy on offsprings’ growth and learning and memorizing functions. Methods: We established the animal model of cadmium exposure by adding 10 mg/L CdCl2 to the drinking water for pregnant mice from 0 d of the pregnancy to the day they gave birth. We divided the offspring into 2 groups: normal control group and cadmium exposure group. We conducted the experiment of Morris water maze at their 5 and 7 week-old and observed their changes in the learning and memorizing functions. Results: The body weight, body length and tail length of offspring newborn in cadmium exposure group were the same as that in normal control group, but the body weight of 3 week-old offspring was lower than that in normal control group. The escape latency of 5 and 7 week-old offspring in cadmium exposure group was longer than that in normal control group. The platform searching time of 7 week-old offspring in cadmium exposure group was shorter than that in normal control group. Conclusion: Cadmium exposure during pregnancy has certain certion influence on offsprings’ growth and learning and memorizing functions.
Keywords: children and adolescents hygiene; pregnancy; low-dose cadmium exposure; offsping development; learning and memorizing functions
 
Title: Subjective evaluation of organization’s operating status from community health staff and factors analysis of turnover intention
Authors: ZHANG Wei, ZHAO Ruiqian, GE Siao, RU Yifu, LI Haiyan, FAN Lihua, SUN Quansheng, LI Heng, LIU Xinyan, ZHANG Yachao, TAN Xiaoyi, SUN Tao
PP: 1165-1173
Abstract: Objective: To survey the subjective evaluation of organization’s operating status of community health staff, and analyze the influencing factors of turnover intention. Methods: Using the questionnaire to collect data, and the influencing factors of turnover intention were analyzed with multivariate Logistic regression analysis. Results: More than 90% of the respondents believe that community health service institutions need organizational learning, organizational innovation and learning experience of business management. More than 37% of respondents believe that the community management model is more flexible, and managers tend to be in the mode of medical technology personnel. More than 70% of the respondents approve operation of institution and development strategies, and believe community health service institutions have a certain development potential. More than 60% of the respondents believe themselves have a development space in community work, more satisfied with current job. 50.8% of the respondents have turnover intention. Conclusion: Community health service workers approve that community needs the business strategy and identity operations and development strategies of organizations. They generally believe the development potentiality of community is enormous, and they have a certain development space. Nearly half of the community health service workers have the turnover intention. Job satisfaction, personal development, and post are important influencing factors of turnover intention of community health service worker.
Keywords: health administration; community health service; organization’s operating status; turnover intention; Logistic regression analysis
 
Title: Preparation of a novel magnetic nanocomposite and its adsorption property for perfluorinated compounds
Authors: ZHOU Yusun, TAO Yun, ZHOU Tingting, SHEN Xiaoli, MEI Surong
PP: 1155-1164
Abstract: In this study, a novel magnetic nanocomposite for selective recognition of perfluorinated compounds (PFCs) was prepared initially based on fluorous and amino monomers. Firstly, according to a facile interfacial coprecipitation method with some modifications, Fe3O4 nanoparticles (NPs) coordinately coated by —COOH were synthesized. Fe3O4 NPs had a nearly spherical shape and a narrow size distribution [(10 ± 5) nm]. The hydrophilic particles could be kept stable for more than 2 weeks in water. Then, using Fe3O4 NPs as substrates, the magnetic nanocomposite (Fe3O4@SiO2-NH2&F13) functionalized with—NH2 and octyl-perfluorinated chain was prepared in one step by a sol-gel process. Fe3O4@SiO2-NH2&F13 has a core-shell structure with silica shell thickness of about 2 nm. In Fourier transform-infrared (FT-IR) spectra, characteristic absorption peaks of the —NH2 deformation vibration around 1 560 cm-1 and the C—F stretching vibration around 1 145 and 1 194 cm-1 demonstrated the presence of —NH2 and C—F bond on surface of Fe3O4@SiO2-NH2&F13. The magnetic nanocomposite has a saturation magnetization value of 40.5 emu•g-1, which makes it be isolated rapidly by an external magnetic field. Fe3O4@SiO2-NH2&F13 exhibited high adsorption capacity and good selectivity for PFCs by electrostatic and fluorine-fluorine interaction with the addition of size exclusion effect. In addition, the composite had a fast sorption rate for PFCs and the adsorption equilibrium could be achieved only in 5 min. Therefore, the proposed magnetic nanocomposite could be used as an effective adsorbent for PFCs trace analysis and their removal from aquatic environment.
Keywords: sanitary inspection; perfluorinated compounds; magnetic nanocomposite; adsorption property; selective recognition
 
Title: Preparation of hollow fiber-supported molecularly imprinted sensors and its application in the direct determination of dopamine in serum samples
Authors: LI Bingbing, ZHOU Feng, HUANG Kai, JING Tao
PP: 1148-1154
Abstract: Objective: Hollow fiber-supported molecularly imprinted sensors were developed for the direct determination of dopamine in cell culture medium, which provided an effective technology to study the mechanism of dopamine. Methods: Molecularly imprinted membrance on the surface of electrode was firstly prepared by using nicotinamide as an environment-friendly monomer to selectively recognize the template molecules. Then, this electrode was attached inside the pores of hollow fibers to prepare an integrated electrode for extraction, concentration and then determinationof dopamine in cell culture medium, simultaneously. Results: Hollow fiber-supported molecularly imprinted composites showed highly selective recognition for dopamine without the interference of ascorbic acid and uric acid coexisting biological fluids. The linear range was changed from 20 nmol/L to 15 μmol/L and the detection limit was 8 nmol/L. The spiked recoveries were changed from 95.1% to 109.2% and the relative standard deviation (RSD) was not higher than 9.6%. Conlusion: Hollow fiber-supported molecularly imprinted sensors can be used for selective, sensitive and direct determination of dopamine in complex samples, which exhibited significant promise in disease diagnosis, medication and fundamental research.
Keywords: sanitary inspection; hollow fiber; molecularly imprinted membrance; electrochemical sensor; dopamine
 
Title: Research on the application of STS method in the Pathogenic Organism and Immunology teaching
Authors: LIU Di, LIU Xuemei
PP: 1142-1147
Abstract: The STS teaching method is used in the course of Pathogenic Organisms and Immunology which taken by five-year junior college students who start nursing study in Dehong Vocational College in 2013. In the practice, the control group of 130 students and experimental group of 164 students were set up. Using the comparison method and questionnaire survey, through the chi-square test, we researched the application and advantages of STS teaching method in teaching the Pathogenic Organisms and Immunology in vocational colleges in the speciality of nursing. The results show that the pass rate of the class which used STS teaching method is 76%, which is significantly higher than that of the traditional teaching class (62%), and the significant differences reached to 0.05 and 0.01 levels. STS teaching method has obvious advantages on teaching students knowledge, arousing interest in learning, improving the study enthusiasm, innovating consciousness as well as awareness of social responsibility.
Keywords: medical microbiology; STS education; pathogenic microorganisms and immunology; application; nursing
 
Title: CIP2A inhibits cell apoptosis in non-small-cell lung cancer
Authors: DONG Qianze, FU Lin, HAN Yong, XING Jilin
PP: 1136-1141
Abstract: Objective: To investigate whether cancerous inhibitor of protein phosphatase 2A (CIP2A) upregulates the expression of cell apoptosis protein Bcl-2 and Bcl-xl. Methods: HBE cell lines and A549 cell lines were used for assays. Cell apoptosis and the expression of Bcl-2 and Bcl-xl were detected using flow cytometry, real-time PCR (RT-PCR) and Western blotting. Results: HBE cell lines were transfected with CIP2A, and A549 cell lines were interfered with CIP2A. Overexpression of CIP2A in HBE cell lines can significantly inhibit cell apoptosis of non-small-cell lung cancer and upregulate the expression of Bcl-2 and Bcl-xl at both protein and mRNA level, whereas, knockdown of CIP2A in A549 cell lines can increase cells apoptosis and inhibit the expression of Bcl-2 and Bcl-xl. Conclusion: CIP2A can regulate the expression of Bcl-2 and Bcl-xl to inhibit cells apoptosis.
Keywords: pathology; non-small-cell lung cancer; CIP2A; Bcl-2; Bcl-xl; cell apoptosis
 
Title: Fibroblast growth factor 5 polymorphisms and non-syndromic orofacial clefts susceptibility in a Chinese Han population
Authors: XU Min, HAN Yue, LI Dandan, MA Lan, PAN Yongchu, WANG Lin
PP: 1130-1135
Abstract: Non-syndromic orofacial clefts (NSOC) are complex and heterogeneous congenital birth malformations. This study was an ongoing hospital-based case-control study that consisted of a large sample size of 599 NSOC cases and 602 healthy controls. Three polymorphisms locating in 3’UTR region of FGF5 were selected (rs3733336, rs4690150 and rs6838203). The results showed that rs3733336 polymorphism was associated with the risk of NSOC.
Keywords: stomatology; FGF5; genetic variants; case-control study; non-syndromic orofacial clefts
 
Title: Isolation of goat bone marrow-derived mesenchymal stem cells: a comparison between two protocols on growth proliferation and biological characteristics
Authors: LÜ Wei, LI Yanmei, ZHONG Ni, ZHANG Wenxia, MA Xiaofang1 BAO Guangjie, KANG Hong
PP: 1121-1129
Abstract: Objective: To investigate the two protocols of goat bone marrow-derived mesenchymal stem cells (gBMSCs) isolation: percoll gradient density separation protocols and holo-bone marrow adherence on purity and biological characteristics in vitro, and to provide large numbers of cells for subsequent experiments. Methods: Bone marrow was sterilely harvested from the thighbones of goat aged about 3-5 months, gBMSCs were isolated by both 2 protocols. The shapes of cells were observed by inverted phase contrast microscopes. The colony forming unit-fibroblast (CFU-F) assay was performed to test those self-renewing. Moreover, the growth curve of passage P3 cells was drawn and surface marker expression of P3 cells was investigated by flow cytometry (FCM). Results: The primary and passaged cells were spindle-shaped and grew in colonies. The growth curves of cultured gBMSCs by both two protocols was like “S” shape. The results of CFU-F assay demonstrated that cells harvestd by holo-bone marrow adherence possessed higher self-renewal capacities. Two kinds of cells were uniform in surface marker expression. Both CD34 and CD44 were positive expression and CD45 was negative expression. Conclusion: Both methods can obtain homogenous gBMSCs with self-renewal capacities. Though cells at P0 retrieved with the percoll protocol had lower proliferation when compared with the holo-bone marrow adherence, there was no significant difference between the two protocols at P3 except self-renewal capacity.
Keywords: stomatology; tissue engineering; goat; bone marrow-derived mesenchymal stem cells; separation of culture
 
Title: Protective effects of Tarasaponin IV on hypoxia/reoxygenation injury in H9c2 cardiocytes
Authors: YANG Minghua, LIU Ying, WANG Min, SUN Guibo
PP: 1115-1120
Abstract: Objective: To investigate protective effects of Tarasaponin IV on H9c2 cardiac myocytes exposed to simulated hypoxia/reoxygenation (H/R). Methods: This experiment were divided into five groups: normal control group (group C); hypoxia/reoxygenation (H/R group) model group; hypoxia/reoxygenation model + Tarasaponin IV low (12.5 mg/L), medium (25.0 mg/L), high (50.0 mg/L) dose group. We use of cardiac H9c2 cells cultured in vitro. Using hypoxia/reoxygenation method of myocardial cell injury induced H9c2 to simulate the clinical myocardial ischemia/reperfusion injury (MIRI), we observe Tarasaponin IV of the hypoxia protective effects of H9c2 myocardial cell injury. Tarasaponin IV intervention by MTT assay cell viability; Hoechst 33342/PI fluorescence staining Tarasaponin IV on hypoxia/reoxygenation-induced apoptosis of myocardial cells and JC-1 fluorescent probe assay H9c2 mitochondrial membrane potential. Results: Compared with the control group, H/R group cell viability was significantly decreased (P<0.01). Compared with the H/R group, cell viability and mitochondrial membrane potential was significantly increased (P<0.05) in Tarasaponin IV group, and myocardial cell apoptosis was significantly reduced (P<0.05). Conclusion: Tarasaponin IV on myocardial hypoxia/reoxygenation injury has a significant protective effect in a dose-dependent manner. The mechanism may be associated with decreased myocardial apoptosis, increased mitochondrial membrane potential.
Keywords: pharmacodynamics; Tarasaponin IV; hypoxia/reoxygenation; H9c2 cardiocytes
 
Title: Effects of dioscin on lipid metabolism, glucose metabolism and liver injury in db/db mice with nonalcoholic fatty liver disease
Authors: XU Lina, YIN Lianhong, QI Yan, PENG Jinyong
PP: 1109-1114
Abstract: Objective: The db/db mice model of nonalcoholic fatty liver disease (NAFLD) was established to investigate the protective effects of dioscin (DIO) on NAFLD. Methods: The mice were randomly distributed into control group, model group and DIO group. Mice in model and DIO group were fed a diet deficient in methionine-choline deficient (MCD) to induce NAFLD. In DIO group, DIO was administered intragastrically (i.g.) at the dose of 80 mg/(kg•d) for four consecutive weeks. Oral glucose tolerance test (OGTT) was carried on for the quantification of the oral glucose tolerance and insulin resistant, and ALT, AST, triglyceride (TG), total cholesterol (TC), blood glucose (BG) and insulin were all detected. At the same time, HE and Sudan III staining were performed to investigate the morphological changes. Results: Compared with mode group, DIO can decrease area under the curve (AUC) in OGTT, and the levels of ALT, AST, TC, TG, BG and insulin were also significantly decreased compared with model group. Morphology results found that fat vacuoles and lipid droplets accumulate appeared in the liver tissue of model group, while the liver steatosis was significantly reduced by DIO adiminstration in DIO group. Conclusion: DIO can significantly improve the liver injury, glucose and lipid metabilism disorder in db/db mice with NAFLD.
Keywords: pharmacodynamics; dioscin; nonalcoholic fatty liver disease; db/db mice; lipid metabolism; glucose metabolism
 
Title: Metabonomic study of MDG-1 on regulation of the lipid meta-bolism disorders in fat mice
Authors: WANG Xu, ZHU Yunyun, SHI Linlin, WANG Yuan, FENG Yi
PP: 1101-1108
Abstract: Objective: The research established a metabonomics-based method to investigate underlying mechanism of MDG-1 in preventing lipid metabolism disorders of fat mice with diet-induced obesity (DIO). Methods: 36 male C57BL/6 mice were randomly divided into three groups (control group, model group and MDG-1 intervened group). After 16-weeks treatment, liver samples were analyzed by ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC/TOF-MS), to find out the potential identification biomarkers by partial least squares-discriminatory analysis (PLS-DA). In addition, biochemical indices of body weight, food intake and blood lipid levels were collected. Results: Compared with model group, significant weight loss and improved lipid levels in MDG-1 intervened group were achieved. In addition, hepatic metabonomic results exhibited that MDG-1 could reverse abnormally expressed metabolites. Meanwhile, 13 potential biomarkers were discovered. Conclusion: MDG-1 could regulate metabolism disorders in DIO model group. Furthermore, the effect may be realized via restricting the biosynthesis of cholesterol as well as some secondary bile acids, and promoting the generation of ketone body.
Keywords: pharmacology; MDG-1 from Ophiopogon japonicus; metabolism disorders; UPLC/TOF-MS; biomarker
 
Title: Simultaneous determination of fifteen active contents in Kanggan granules by HPLC-DAD
Authors: XU Chunfang, LIN Xinqiang, XIE Xinyue, XU Wen, HUANG Mingqing
PP: 1081-1089
Abstract: In this study, a high performance liquid chromatography-diode array detector (HPLC-DAD) method for simultaneous determination of 15 active contents in Kanggan granules, which are gallic acid, neochlorogenic acid, protocatechuic acid, 4-hydroxybenzoic acid, oxypaeoniflora, chlorogenic acid, swertiamarin, cryptochlorogenin acid, albiflorin, paeoniflorin, rutin, pentagalloylglucose, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C. The 15 active compounds were simultaneously separated by a Ultimate C18 (150 mm × 4.6 mm, 5 μm) column with acetonitrile (B)-0.1% formic acid (A) as the mobile phase and a gradient elution mode set with 0.8 mL/min of flow rate, 25℃ of column temperature and 10 μL of injection volume. The results showed that HPLC-DAD quantitative methodology was validated. Satisfactory linearity was achieved with wide linear range and fine determination coefficient (r>0.999 0), and the overall recoveries were ranged from 95.70%-104.74% with the relative standard deviation (RSD) ranging from 1.90%-4.96%. It is the first report about simultaneous analysis of 15 major components in Kanggan granules by using HPLC-DAD method, which is a simple, specific, fast and accurate method for quality control of Kanggan granules.
Keywords: science of Chinese pharmacology; Kanggan granules; HPLC-DAD; quality control
 
Title: Progresses and applications of DNA barcoding in the research of traditional Chinese medicine resources and identification
Authors: LIU Jie, TIAN Xiaoxuan
PP: 1075-1080
Abstract: In this review, several studies about DNA barcoding on traditional Chinese medicine (TCM) were summarized. Furthermore, through the collation of relevant literature, the application of DNA barcoding on the discovery of cryptic TCM species and the identification of Chinese patent medicine preparation based on this technique were discussed. We extend the potential applying prospect of DNA barcoding in TCM resources and TCM identification.
Keywords: science of Chinese pharmacology; DNA barcoding; review; traditional Chinese medicine resources; traditional Chinese medicine identification