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ISSN 1674-2850
CN 11-9150/N5
 
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March 15,2015
Volume 8,Issue 5
Pages -
Subject Area:Animal Reproduction,Cardiovascular Diseases
 
Title: Morphological and physiological characteristics of Seriphidium transiliense seed and response of endozoochorous by sheep
Authors: LU Weihua, WAN Juanjuan, YANG Jiejing
PP: 500-509
Abstract: To determine whether livestock can disperse plant seeds by endozoochorous or not, we collected dominant livestock dung and tested germinative plant seeds of quantity and variety. At the same time, we took constructive species-Seriphidium seeds and sheep as research object to study changes of germination of seed number and seed morphology, physiology characteristics after endozoochorous by feeding seeds. The results showed that the three main grazing livestock could disperse seeds of many plants, but the number and variety was different. Most seeds were excreted in 6-36 h after feeding. After seeds passage sheep tract, some seeds could still maintain their vitality, but the morphological characteristics were changed. The absorbent, sticky sand performance of seeds decreased significantly, while dehydration performance increased as artificial eliminate natural seed coat, the natural seed coat had completely disappeared, which caused the change of germination behavior with lower various germination indexes, and affected the growth of seedlings. But the natural seed coat also protected the seeds free from deep damage of digestion.
Keywords: range science; Seriphidium transiliense seed; morphological and physiological characteristics; endozoochorous
 
Title: Fast detection technology research of silkworm as biological indicator of agricultural contaminant
Authors: LIU Xinju, CAI Zizheng, MIAO Yungen
PP: 495-499
Abstract: We took the serious polluted water from Hangzhou Qiantang river and Beitang river. The mulberry leaves were dipped in polluted water, and fed the silkworm Bombyx mori L. The chronic symptoms, mortality, body weight of sleeping worms were investigated. Also we determined the acetylcholinesterase (AChE) and glutathione-S-transferase (GST) activity to assess the technical feasibility of the silkworm as biological indicator of agricultural contaminant detection. Experimental results showed that the effects of different sources of polluted water performed on silkworm poisoning. Although starting treatment from the 2nd instar, but until the 4th sleeping stage, the body weight of worms fed with mulberry dipped in the polluted water was lower than that of control. However the AChE and GST, which was as resistance index, had been significantly reduced in the three sleeping period. Therefore, with respect to the phenotype, silkworm AChE and GST activity can be used as indicators for pre-detecting the contaminants in agricultural habitats.
Keywords: sericulture science; Bombyx mori L; agricultural contaminant; acetylcholinesterase; glutathione-S-transferase; biological indicator
 
Title: H1c gene expression during early embryonic development stages of water buffalo
Authors: HUANG Gaobo, QUAN Li, LIU Yuxian, LU Shengsheng
PP: 488-494
Abstract: Water buffalo is an important livestock in south China. However, the reproductive ability of buffalo is low. The H1c gene has been shown to affect the early embryonic development in some species, but little is known about its function in early embryonic development of buffalos. In this study, real-time quantitative-PCR (qRT-PCR) was used to profile the expression of H1c mRNA in early embryonic development of buffalo. Our results showed that the expression of H1c mRNA increased at the early stages of buffalo embryos. Therefore, we suggest that H1c may play an important role in in vitro fertilization（IVF）embryonic development of buffalo.
Keywords: animal reproduction; water buffalo; early embryo; linker histone; H1c gene
 
Title: Study on synthesis and in vitro release of mequindox nanoparticles
Authors: BAO Guangming, HU Chunyan, LIU Baosheng, YUAN Houqun, NIE Haijing, XIAO Xuefei, YU Caiyun, ZHU Si
PP: 482-487
Abstract: Objective: To overcome the shortcomings of bitterness and hydrophobicity, the mequindox nanoparticles (MEQ@MSN) is planned to be studied for injection. Methods: MEQ@MSN were prepared with synthesized MCM-41 type mesoporous silica nanoparticular (MSN) which had characteristics of high surface area, total pore volume and uniform mesoporous structure. Both structures of MSN and MEQ@MSN were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), nitrogen adsorption-desorption and Fourier transform infrared spectra. Results: Mequindox (MEQ) was successfully loaded into the pores of mesoporous silica, and the formed the dispersed spherical particles with an average diameter of about 100 nm. In vitro studies showed that mequindox could be quickly released from MEQ@MSN, which made sure of mequindox to start work immediately. Conclusion: The MEQ@MSN is expected to be used as an injection of mequindox for treating of sick aquaculture animal.
Keywords: veterinary medicine engineering; nano medicine; mequindox; drug loading; in vitro release
 
Title: Research and application progress of point embedding therapy in sport fatigue
Authors: ZHU Hao, WANG Xuefei, LIU Dandan, ZHANG Tongjun, YANG Ying
PP: 474-481
Abstract: Sport fatigue is the research hotspot of prevention and cure in exercise physiology as well as sport medicine, the research on sport fatigue is of great theoretical significance. Furthermore, it has practical application value for the guidance of exercise training to improve sport fatigue and improve exercise tolerance. The catgut implantation at acupoint may induce and stimulate the regulate system of the body in varying degrees, so the imbalances of functional relationships tend to normalize and maximize expression. This review is to summarize the research results of acupoint catgut embedding on anti-fatigue, it also has provided the theory basis for the research of horse racing for removing sport fatigue and improving athletic performance.
Keywords: traditional Chinese veterinary science; point embedding therapy; review; sport fatigue
 
Title: Evaluation of consistency of stoke volume between PC MR and cardiac MR using Bland-Altman analysis
Authors: GUO Li，GUO Hao，YUAN Yong，ZENG Weiyong，YUAN Shuguang，YANG Nan
PP: 468-473
Abstract: Objective: To investigate the application of Bland-Altman analysis for evaluation of consistency of stroke volume (SV) which were measured by phase contrast magnetic resonance (PC MR) and cardiac MR. Methods: SV was determined by measuring the blood flow in the beginning of aorta and which by measuring the change of left ventricular of 22 healthy volunteers within each cardiac cycle. The clinical consistency of PC MR and cardiac MR was respectively evaluated by paired-samples t test, correlation and Bland-Altman analysis. Results: The paired-samples t test showed that the SV measured by PC MR and cardiac MR had statistical differences (P<0.05). The correlation showed there was good relationship between the values of SV measured by PC MR and cardiac MR (R=0.95). However, Bland-Altman analysis revealed that the values of SV measured by PC MR were less than cardiac MR. 100% of the points were in the limits of agreement, the maximum absolute value of difference (9.48 mL) was in the acceptable range of physiological fluctuations of SV and clinical admissible error. Conclusion: Compared with the paired-samples t test and correlation, Bland-Altman analysis can give more satisfactory result, so it is a perfect method for evaluation of consistency.
Keywords: medical imaging; Bland-Altman analysis; stroke volume; phase contrast; heart volume
 
Title: Telbivudine improves estimated glomerular filtration rate in patients with chronic hepatitis B
Authors: QI Xun, WANG Jinyu, MAO Richeng, ZHANG Jiming
PP: 458-467
Abstract: Objective: To evaluate the long-term effects of nucleoside/nucleotide analogues on estimated glomerular filtration rate (eGFR) in Chinese chronic hepatitis B (CHB) patients. Methods: This prospective cohort study included 275 CHB patients. Patient subgroups included those treated with lamivudine (n = 50), adefovir (n = 60), telbivudine (n = 68), and entecavir (n = 61); untreated patients (n = 36) served as control. The data was calculated by Cockcroft-Gault (CG) and MDRD formulas. Results: After an average follow-up duration of 23 months, eGFR calculated by CG and MDRD formulas increased by 18.35 mL/min and 19.34 mL/min (P < 0.000 1) in the telbivudine group, respectively, and decreased by 10.95 mL/min and 12.17 mL/min (P = 0.000 1) in the adefovir group, respectively. Even if renal function was normal or mildly impaired at baseline, eGFR increased significantly more in the telbivudine group than in the other groups (P < 0.000 1). More patients in the telbivudine group (31%) had a ≥20% elevation in eGFR (CG) than the other groups (P < 0.000 1). More patients in the adefovir group (23%) had a ≥20% decrease in eGFR (CG) than the other groups (P < 0.000 1). Conclusion: Prolonged telbivudine therapy results in improved eGFR, while adefovir therapy is associated with decreased eGFR. Lamivudine and entecavir therapy do not significantly influence eGFR.
Keywords: infectious disease; nucleoside/nucleotide analogues; estimated glomerular filtration rate; chronic hepatitis B
 
Title: Effect of decidual TLR4 on regulatory T cell function in patients with unexplained recurrent spontaneous abortion
Authors: BAO Shihua, SHUAI Wen
PP: 449-457
Abstract: Objective: To investigate the expression of Toll-like receptor (TLR) on T cells in deciduas and their effect on immunosuppressive capacity of regulatory T cells (Treg) in patients with unexplained recurrent spontaneous abortion (URSA). Methods: The expression of TLR mRNA on Trge cells was examined by RT-PCR. The proportion of TLR4 in Trge cells was detected by flow cytometry (FCM). The effects of TLR4 on Treg cell proliferation, immune suppression, and the regulation of Treg cells to natural killer (NK) cell function were observed using the isolated Treg cells by TLR specific ligand stimulation in vitro. Results: Compared with control group, higher expression of TLR4 was detected on Trge cells in deciduas (P=0.004). LPS could enhance the immunosuppressive capacity of Trge cells, and also could facilitate the proliferation of Trge cells. Engagement with LPS, purified Trge cells could inhibit the function of NK cells in vitro without the presence of other immunological cells. However, the immunosuppressive capacity of Trge cells was decreased with the existence of other immunological cells. Conclusion: There may be an abnormal activation of TLR4 pathway in URSA, which led to the decrease of immunosuppressive capacity of T cells and the abnormal activation and toxicity increase of NK cell, and therefore, this led to imbalance of maternal fetal interface state of immunological tolerance.
Keywords: obstetrics and gynaecology; recurrent spontaneous abortion; regulatory T cells; natrual killer cells; Toll-like receptor
 
Title: Effect of JNK signaling transduction pathway in vascular endothelial cell senescence induced by AngⅡ
Authors: SHAN Haiyan, WEI Xiaojie ,YU Kai, ZHAO Xin, YU Xiaosong
PP: 443-448
Abstract: Objective: To explore the effect of angiotensinⅡ(AngⅡ) on senescence of human umbilical vein endothelial cells (HUVECs) and the expression change of c-Jun-NH2-terminalkinase (JNK) and nuclear factor-κB (NF-κB). Methods: HUVECs were cultured in RPMI1640 medium including AngⅡ (10-6 mol/L). β-galactosidase (β-gal) staining and flow cytometry were used to identify cell aging status. By means of Western-blotting, the expression of JNK, p-JNK and NF-κB was observed. Results: The number of positive cells which were identified by β-gal staining was significantly higher in AngⅡ-induced cells (81.09±6.72)% than those in the control cells. Flow cytometry showed that the cell cycle was at G0-G1 stage. Compared with control group, p-JNK, NF-κB protein expression increased markedly (P<0.05) induced by AngⅡ for 16, 32, 48 h, and the expression level of p-JNK reached the peak at 32 h (P<0.01). Furthermore, when the expression of p-JNK increased, the NF-κB expression also increased significantly. Conclusion: There is a probability that activated JNK/NF-κB signaling transduction pathway is involved in the process of endothelial cell senescence induced by AngⅡ.
Keywords: cardiovascular diseases；angiotensinⅡ; endothelial cell; vascular aging; c-Jun-NH2- terminalkinase; nuclear factor-κB
 
Title: Effects and mechanism of high glucose on calcium signaling in atrial myocytes
Authors: ZHAO Can, WU Yongquan
PP: 437-442
Abstract: Objective: To investigate the effect of high glucose on calcium signals of atrial myocytes and its mechanism. Methods: The experiment based on isolated primary cultured SD atrial myocytes rats were divided into two parts, the first section was divided into four groups: 1) L-glucose (LG) group; 2) normal group (NG); 3) high glucose (HG) group; 4) HG+uric acid (HG+U) group. The second section was divided into three groups: 1) ONOO group; 2) DC-ONOO group; 3) ONOO +U group. We observed the change of calcium signaling, calcium channel RYR2 mRNA and the expression of its upstream regulatory gene FKBP12.6 mRNA. Results: Calcium transient amplitude was larger in the HG group compared with the NG group. Application of uric acid significantly decreased the calcium transient amplitude whereas exposure of myocytes to ONOO markedly increased. The expression of RYR2 mRNA in the HG group was decreased when compared to the NG group. Incubation in uric acid increased the expression of RYR2 mRNA, whereas ONOO decreased the amount of RYR2 mRNA. The expression of FKBP12.6 mRNA was decreased in the HG group compared to NG treated cells. Conclusion: High glucose increases the release of calcium, the mechanism may be associated with high glucose-induced oxidative stress on calcium channel.
Keywords: internal medicine; calcium signaling; hyperglycemia; oxidative stress
 
Title: Biodegradable waterborne polyurethane PEGU25 incorporated in Bmap-18, ciprofloxacin or quaternary ammonium salt in the inhibition of the formation of bacterial biofilm in vitro
Authors: WANG Jianzhong, LIU Qinyu, WANG Kunjie, LI Hong
PP: 425-436
Abstract: Objective: To test the inhibition effects of antibacterial peptide-coating-biodegradable waterborne polyurethane membrane on the biofilm formation in vitro and prove its proficiency as new antibacterial biological material. Methods: Bamp-18, ciprofloxacin and quaternary ammonium salt were incorporated in PEGU25 (1 mg/cm2), respectively. These membranes were cultured with Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC25922) and Proteus mirabilis (ATCC12453) separately in culture medium, artificial urine or in vitro model of artificial bladder for 2, 4, 7 d. We evaluated the ability of inhibiting bacterial biofilm formation by living bacterium colony count, confocal fluorescence microscope combining Syto-9/PI fluorescence staining, and scanning electron microscopy (SEM) method. Results: Compared with the blank PEGU25 membrane, PEGU25 membrane with ciprofloxacin, quaternary ammonium salt, and Bmap-18 could effectively inhibit bacterial biofilm formation and the formation of calcium magnesium phosphate calm hard bacterial biofilm at the same time. Under the condition of in vitro model of artificial bladder, the formation of hard calcium magnesium phosphate calm inside and outside the duct lumen could be effectively suppressed and the obstruction of catheter could be delayed well. In which, PEGU25 with ciprofloxacin performed the best, and Bmap-18 had similar effect to quaternary ammonium salt. Conclusion: PEGU25 loading Bmap-18, quaternary ammonium salt and ciprofloxacin can well inhibit bacterial biofilm formation, which has the huge potential with service in clinic.
Keywords: surgery; antimicrobial peptide; bacterial biofilm; polyurethane; drug-coating- polyurethane material
 
Title: Protective effects of p53 gene in the pulmonary arterial remodeling
Authors: LI Gang, ZHU Yaobin, LIU Aijun, XU Yulin, LI Bin, LIU Yinglong
PP: 417-424
Abstract: Objective: Similarities exist between cancer and pulmonary arterial hypertension (PAH), and the present research is to investigate the possible effects of an anti-oncogene p53 in the process of pulmonary arterial remodeling in PAH. Methods: Forty male SD rats were randomly divided into four groups including control group, p53 control group, PAH group and p53 preventive group. Severe pulmonary arterial hypertension was induced by monocrotaline injection one week following left pneumonectomy and Adeno-p53 (with wild type p53 gene) was transfected into lung by tracheal inhalation. p53 protein expression were detected by Western blotting analysis, mean pulmonary arterial pressure (mPAP) were measured by catheterization, the right ventricular hypertrophy index (RVHI) was evaluated by the ratio of right ventricular to left ventricular plus septum weight, media hypertrophy and neointimal formation were analyzed by pathological method. The proliferation of pulmonary smooth muscle cells were analyzed by stain for α-smooth muscle actin and proliferating cell nuclear antigen (PCNA) with immunohistochemical method in small pulmonary artery. Results: The expression of p53 protein were lower in the PAH group than that in the control group after 3 week treatment (P＜0.05), while the level of p53 protein were increased in both p53 control group and p53 preventive group (P＜0.05). The mPAP and right ventricular hypotrophy index (RVHI) decreased in p53 preventive group when comparing with PAH group (P<0.05), medial hypertrophy and intimal hyperplasia reduced. The positive α-actin staining were widely distributed on small pulmonary arteries, and the PCNA positive rate were lower in p53 preventive group than in PAH group (P＜0.05). Conclusion: p53 played an important role in the development of severe PAH, and overexpression of p53 gene by transfection prevented the formation of severe PAH. Inhibition of the smooth muscle cells proliferation was one of its possible mechanism.
Keywords: surgery; pulmonary arterial hypertension; p53 gene; pulmonary arterial remodeling
 
Title: A retrospective analysis on the clinical features of 6 cases of non-Hodgkin's lymphoma
Authors: ZHANG Xin, SONG Peng, DING Yuangang
PP: 413-416
Abstract: Objective: To study the clinical diagnosis and treatment of non-Hodgkin's lymphoma (NHL) in clinical diagnosis and treatment. Methods: We made a retrospective review of 6 cases of NHL clinical data from Department of Blood Internal Medicine, The First Affiliated Hospital of Harbin Medical University, from March 2010 to August 2011, including 4 males and 2 females, and discussed them combined with the relevant literatures. Results: Four patients received chemotherapy and anti-bacterial-fungal treatment, one patient received single-agent chemotherapy and anti-bacterial-fungal treatment, one patient received anti-bacterial-fungal treatment, and discharged in stable condition. Conclusion: Since NHL is very sensitive to chemotherapy, and the chemotherapy technological is advanced, chemotherapy alone can rapidly alleviate the NHL symptoms.
Keywords: oncology; non-Hodgkin's lymphoma; clinical analysis; chemotherapy
 
Title: Histologic classification, grading and staging of tumor
Authors: YANG Jun，LI Zongfang
PP: 407-412
Abstract: The histologic classification, grading and staging are the most important three parameters for evaluating the biological behavior, the ability of invasion and metastasis, and the clinical progression of tumor. The histologic classification and grade are intrinsic parameters of tumor, they are used to reveal the source and biological behavior and the ability of invasion and metastasis of tumor. Every malignant tumor has its unique biological behavior and the ability of invasion and metastasis dependent on its classification and grade. The stage is a visible important external parameter for revealing the ability of invasion and metastasis and the degree of tumor progression, which depends not only on the histologic classification and grade and the degree of invasion and metastasis, but also obviously limited by the point-in-time for the patient going to a doctor. Tumor staging is used to mean the progression phase and the expected survival time for patients with malignant tumor. However, in the coming era of tumor individualized treatment, the traditional tumor classification, grading and staging already cannot satisfy the needs of it. So it is important for simplifying the tumor classification standard, adding the quantifiable parameters, increasing the level of tumor molecular diagnosis, which should provide more accurate molecular biology information for individualized treatment of tumor, and guide to make the therapeutic schedule and supervise the curative effect. This will undoubtedly become the development direction and goal of malignant tumor classification, grading and staging.
Keywords: oncology; tumor; review; classification; grading; staging