Home > Highlights of Sciencepaper Online > Current Issue

 
 
 
ISSN 1674-2850
CN 11-9150/N5
 
Current Issue
Previous Issue  |  Next Issue
December 15,2014
Volume 7,Issue 23
Pages -
Subject Area:Chemistry of Traditional Chinese Medicine
 
Title: Effects of high glucose in vitro and in vivo on the inflammatory factors of the TLR4 pathway
Authors: XIE Weidong
PP: 2394-2398
Abstract: Objective: This paper aims to investigate the different expressions of inflammatory factors of the Toll-like receptor 4 (TLR4) pathway in monocyte-macrophages from mouse’s peritoneal cavity induced by high glucose in vtrio or diabetes in vivo. Methods: For in vitro trials, monocyte-macrophages from normal mouse’s peritoneal cavity were cultured by high glucose and then total RNAs were extracted for quantitative-PCR (q-PCR) of inflammatory factors from the TLR4 pathway. For in vivo trials, monocyte-macrophages from diabetic mouse’s peritoneal cavity were induced by streptozotocin (STZ) and then total RNAs were extracted by q-PCR of inflammatory factors from the TLR4 pathway. Results: In the trials in vitro, high glucose only up-regulated the expressions of IL1a and IL1b did nothing to the other inflammatory factors of the TLR4 pathway. However, diabetic mice showed higher expressions of most of the inflammatory factors of the TLR4 pathway than normal controls in the trials in vivo. In addition, these up-regulations induced by high glucose or diabetes were far less than those treated by lipopolysaccharide (LPS). Conclusion: High glucose did not cause acute and extremely inflammatory responses but led to a slight increase of inflammation in monocytes or macrophages. High glucose may be not a key factor to responsible for inflammation responses but may combine with other factors to contribute low-grade systematical inflammatory responses in vivo in the case of diabetes. Future strategy should aim to control inflammation directly instead of hyperglycemia in diabetes.
Keywords: other subject of basic medicine; diabetes; inflammation; Toll-like receptor 4
 
Title: Characteristic identification of a long non-coding RNA in aggressive neuroblastoma
Authors: YU Meng, ZHU Yuyan, DONG Wanwei, YANG Wei, GUO Xiaochong, ZHENG Zhihong
PP: 2389-2393
Abstract: Objective: To identify the characters and functions of ncRAN, which was screened from clinical sample in aggressive neuroblastoma (NB). Methods: The length of ncRAN mRNA was detected by Northern blotting. The expression of ncRAN on protein level was checked by autoradiography. The characters of ncRAN were identified by real time-PCR (RT-PCR), siRNA and soft agar colony formation. Results: The data showed that ncRAN could express the full-length gene sequences at RNA level, but it did not code for any protein products at the protein levels. The molecular biological experiment results suggested that ncRAN was a novel non-coding gene with oncogenic function. Conclusion: As a novel long non-coding RNA (lncRNA), ncRAN, acted as an oncogene, was associated with progression of NB.
Keywords: other subject of basic medicine; neuroblastoma; ncRAN; long non-coding RNA; oncogene
 
Title: Study on effect of rhG-CSF in intestinal form of acute radiation sickness in C57BL/6 mice
Authors: LIU Wei, QI Dandan, GU Yongping, CUI Fengmei, CHEN Qiu
PP: 2383-2388
Abstract: Objective: To investigate effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) in intestinal form of acute radiation sickness (ARS) in C57BL/6 mice by the animal model of acute radiation damage. Methods: Healthy C57BL/6 mice were randomly divided into 5 groupes, namely the normal grope, 10 Gy radiation group, rhG-CSF administration group of high, middle, low concentration after 10 Gy radiation. The survival curves of mice were drew after irradiation. 3.5 d after radiation, bone and intestine were taken to observe the pathological change and the number of Cleaved Caspase-3 positive cells were counted. Results: The results showed that 12 h after 10 Gy total body irradiation giving the high concentration of rhG-CSF, the survival rate increased significantly. However, 3.5 d after irradiation, comparing high concentrations of rhG-CSF administration group with 10 Gy radiation alone group, bone tissue and the length of intestinal villus did not change significantly, but the number of Cleaved Caspase-3 positive cells in crypts reduced. Conclusion: The research revealed that rhG-CSF reduced the number of Cleaved Caspase-3 positive cells in crypts and had a protective role in the intestinal form of acute radiation sickness in C57BL/6 mice.
Keywords: radiation medicine; recombinant human granulocyte colony stimulating factor; intestinal form of acute radiation sickness; radio protection
 
Title: Effects of Guifutongjing granula on hemorheology of congealing cold and blood stasis rats
Authors: YU Ruihong, CAO Lei
PP: 2379-2382
Abstract: Objective: To observe the effects of Guifutongjing granula on hemorheology of congealing cold and blood stasis rats. Methods: We make the model of congealing cold and blood stasis in rats, and intragastric administration Guifutongjing granula for 1 week, then collect blood by abdominal aortic method and determine blood specific and reductive viscosity, erythrocyte deformation index and rigid index, hematocrit, plasma viscosity, fibrinogen and erythrocyte sedimentation rate. Results: Compared with normal group, high-shearrate blood viscosity, low-shearrate blood viscosity and low-shearrate reductive viscosity of model group increased greatly (P<0.01), high-shearrate reductive viscosity and erythrocyte rigid index increased to some degree (P<0.05). Compared with model group, 0.5 g/kg and 1.0 g/kg groups of Guifutongjing granula reduce high-shearrate blood viscosity, low-shearrate blood viscosity and low-shearrate reductive viscosity (P<0.01). Conclusion: Guifutongjing granula can improve the blood stasis state of congealing cold and blood stasis rats.
Keywords: pharmacology; Guifutongjing granula; syndrome of congealing cold and blood stasis; hemorheology
 
Title: Effects of baicalein on the enzymes of myocardial tissue of myocardial ischemia-reperfusion injury in rats
Authors: HUANG Yongpan, YE Lan, WANG Heng, SHI Ganggang
PP: 2373-2378
Abstract: Objective: To investigate the protective effects of baicalein on rat heart ischemia-reperfusion injury. Methods: Rat heart was occluded the left anterior descending coronary artery for 30 min and then was restored blood perfusion for 30 min. Different dosages (40, 80, 160 mg/kg, respectively) of baicalein were intravenously injected before heart ischemia. The activity of plasma creatine kinase (CK), creatine kinase isoenzyme B (CK-MB), lactate dehydrogenase (LDH), α-hydroxybutyrate dehydrogenase (HBDH), grutamic oxalacetic transaminase (GOT) and superoxide dismutase (SOD), and the content of malondiadehyde (MDA) were measured. The pathologic changes were also observed. Results: Baicalein could reduce the release of CK, CK-MB, LDH, HBDH and GOT of cardiac damage, and protect the activity of SOD, while decreased the MDA product and the decrease showed a dose-effect relation. Conclusion: Baicalein may exert an apparent protective role against rat heart ischemia-reperfusion injury.
Keywords: pharmacology; baicalein; myocardial reperfusion injury; creatine kinase isoenzyme MB; superoxide dismutase; malondialdehyde
 
Title: Bronchus hyperresponsiveness induced by atmospheric PM2.5
Authors: WANG Rong, XIAO Xue, CAO Lei, SHEN Zhenxing, CAO Yongxiao
PP: 2367-2372
Abstract: Objective: To explore the effects of DMSO-soluble fine particulate matter (PM2.5) on the contractile function in isolated rat bronchial segments and reveal its mechanism. Methods: The rat bronchi were dissected and cut into about 1 mm long segments. The bronchi were cultured in the different dose of PM2.5 and for different times. The contractile responses to carbachol (M receptor agonist) were recorded using a sensitive myograph system, and the consponding contractile time and dose effect curves were established. Results: Carbachol induced an dose-dependent contraction in rat bronchial segments. Compared to fresh, after organ culture with DMSO for 12 h or 24 h, carbachol induced the maximal contraction (Emax) and the negative logarithm of the concentration that produces 50% of the maximal contractile effect (pEC50) had not significant difference. However, after organ cultured with DMSO for 48 h, the contractile dose curves of carbachol induced contraction response shifted left significantly (P<0.01), Compared to DMSO, culture with PM2.5 for 12, 24 or 48 h, the contraction response were enhanced by carbachol in rat bronchial segments, and it was the largest for 24 h culture. Moreover, when bronchial segments were cultured with 0.3, 1.0 or 3.0 mg/L PM2.5 for 24 h,the contraction response were enhanced by carbachol, and the corresponding Emax were (149.4±9.9)%, (195.4±10.0)% and (143.9%±7.7)%, respectively. Conclusion: PM2.5 increased the M receptor-mediated contractile responses in rat bronchi and induced airway hyperresponsiveness.
Keywords: pharmacology; airway hyperresponsiveness; wire myograph; fine particulate matter
 
Title: Antibacterial activity of an inhibitor W-23 of Staphylococcus epidermidis tryptophanyl-tRNA synthetase
Authors: WU Yang, MAO Jialin, QU Di
PP: 2362-2366
Abstract: Objective: To investigate the antibacterial activity of W23, an inhibitor of Staphylococcus epidermidis tryptophanyl-tRNA synthetase (WRS). Methods: We determine the minimal inhibitory concentration (MIC) of W-23 to S.epidermidis, S.taphylococcus and Escherichia coli using broth dilution method and detect the effect of W-23 in combination with ampicillin or chloramphenicol on S.epidermidis growth. Results: The MIC of W-23 to Staphylococcus species is about 2-4 mg/L and it is a bacteristatic agent. Combination of W-23 and ampicillin has synergistic effect on growth of methicillin-resistent S.epidermidis. Conclusion: W-23 has potent antibacterial activity against Staphylococcus and has a potential value in new drug development.
Keywords: medical microbiology; Staphylococcus; tryptophanyl-tRNA synthetase; inhibitor W-23
 
Title: Effects of Torin1 and CQ on xenophagy in Salmonella-zebrafish model
Authors: LI Jinling, WU Shuyan, LI Yuanyuan, HUANG Rui
PP: 2355-2361
Abstract: Objective: To explore the influence of activation agent Torin1 and autophagy inhibitor chloroeuine (CQ) on xenophagy of zebrafish larvae infected by Salmonella typhimurium of autophagy. Methods: Zebrafish larvae at 72 h post-fertilization were exposed to different doses of S.typhimurium. Bacterial growth curves, zebrafish survival curves and Western blotting were used to determine the suitable concentration of Torin1 and CQ. Bacteria enumberation and Western blotting were used to explore their impact on xenophagy of zebrafish larvae. Results: The larvae were treated with 0.4 μmol/L Torin1 for 24 h, the expression of autophagy protein Lc3-Ⅱwas significantly increased, but p62 was reduced. Lc3-Ⅱ and p62 expression levels of the larvae treated with 50 μmol/L CQ for 24 h were contrary to the above. Conclusion: Torin1 and CQ have the same influence on xenophagy of zebrafish larvae infected by S.typhimurium as it has on autophagy in mammalian cells.
Keywords: medical microbiology; Salmonella typhimurium; zebrafish; autophagy
 
Title: Experimental study on the differentiation of synovial mesenchymal stem cells into osteoblasts and chondrocytes
Authors: ZENG Jianchun, FAN Yueguang, ZENG Yirong, LI Jie, LI Feilong
PP: 2348-2354
Abstract: Objective: To explore the differentiation features of synovial mesenchymal stem cells (SMSCs) into the osteoblasts and chondrocytes. Methods: The fourth generation of SMSCs were seeded in 3 6-well plates by 5×106 cells/cm2, in one of the plates, SMSCs were induced to chondrocytes, in the remaining two boards, SMSCs were induced to differentiate into osteoblasts. Four holes in each plate, two holes were blank holes, and the other two holes were the observation group. 2 d later, the culture media were changed with inducing fluids. The control group was added L-DMEM medium containing 10% fetal bovine serum (FBS), the inducting group was added inducing agents on the basis of the L-DMEM medium [osteogenic group containing β glycerophosphate 10 mmol/L, dexamethasone 0.1 μmol/L, vitamin C 50 mg/L; the chondrogenic group containing transforming growth factor β1 (TGF-β1) 10 μg/L, dexamethasone 0.1 μmol/L, vitamin C 50 mg/L]. On the 14th day, a 6-well was removed for Alcian blue staining, a 6-well plate was done for alkaline phosphatase staining. On the 21st day, the remaining 6-well plate was done for Alizarin red staining. Results: Volume of SMSCs increased after induced by chondroinductive medium, the spindle changed into oval shape, some nuclei deviated and showed like kidney-shape. After 14 d, the surrounding cells matrix could be seen secreted by SMSCs, and Alcian blue staining was positive. After induced by osteogenic medium, SMSCs changed into oval from spindle in shape, volume increased significantly, on 14th day granular substance was visible on the surface of colony, alkaline phosphatase staining was positive, Alizarin red staining was also positive on 21st day, showing a characteristic of osteoblasts. Conclution: SMSCs were widely present in the synovial tissue, they showed the characteristics of stem cells which can be induced to differentiate into osteoblasts and chondrocytes under certain conditions.
Keywords: medical cell biology; synovial mesenchymal stem cells; osteoblasts; chondrocytes
 
Title: Impact of mast cell function on acupuncture effect in mouse “Zusanli” point area
Authors: GAO Yi, LI Zhongzheng, YAN Lijuan, ZHAO Xue, GUO Yi
PP: 2340-2347
Abstract: Objective: To investigate the relationship between mast cells and acupuncture effect. Methods: Taking Kunming mice gastric emptying rate as the effect indicators, gastric emptying rate was observed in each group of mice for 20 min in the process of changing the needle row. While tissue sections stained by acupuncture, acupoint injection of cromolyn sodium, compound 48/80 respectively. The number and rate of change of degranulation of mast cells in mouse mast cells acupuncture points were observed. Results: Cromolyn sodium injection could significantly reduce the number of points at the mast cells and mast cell degranulation rate, then acupuncture could increase the number of points at the mast cells and mast cell degranulation rate. After injection of compound 48/80 point at mast cells, the number and mast cell degranulation rates were significantly increased, while the acupuncture phenomenon mast cell degranulation rate improved significantly. Conclusion: This study showed that acupuncture tissue mast cells played an important role in the production process of acupuncture effect, and the mast cell degranulation process was involved in the generation of acupuncture effect.
Keywords: science of acupuncture and moxibustion; Zusanli; mast cell; acupuncture effect; gastric emptying rate
 
Title: Study on test-retest reliability of regional homogeneity of the resting-state fMRI for the patients with over-anxiety condition
Authors: WU Hongyun, QI Xianghua
PP: 2337-2339
Abstract: Objective: To measure test-retest reliability of the resting-state functional magnetic resonance imaging (fMRI) for the patients with over-anxiety condition (OAC). Methods: 10 patients with OAC were arranged to accept the resting-state fMRI scan, and were conducted a second scan after 2 d interval. Regional homogeneity (ReHo) was adopted to calculate difference and the intraclass correlation coefficients (ICC) of the whole brain according to the 2 measurements. Results: Under the premise of AlphaSim correction, the connection rule being edge connection and the condition being P<0.05, ReHo value of left superior temporal gyrus reduced after the retest. When P<0.01 or the connection rule was the angle connection, there was no difference. ICC of the whole brain 0.746 meant substantial test-retest reliability. Conclusion: The good test-retest reliability in OAC patients displaying the resting-state fMRI is an important method for researching on cognitive neuroscience.
Keywords: traditional Chinese medicine; functional magnetic resonance imaging; resting-state; test-retest reliability; over-anxiety
 
Title: Effect of Zuogui pill on expressions of TGF-β1/Smad4 mRNA in bone tissues of PMOP rats
Authors: REN Yanling, LÜ Haibo, LIU Liping, LI Yaling
PP: 2331-2336
Abstract: Objective: To study the effect of Zuogui pill on the expression of TGF-β1/Smad4 mRNA in bones and the mechanisms of preventing and curing osteoporosis. Methods: Female SD rats were distributed into normal group, sham-operation group, ovariectomized (OVX) group, high, medium and low dose of Zuogui pill groups, and nilestriol group. Rats of overiectomy-induced osteoporosis model were adopted. Rats were orally administrated physiological saline (negative group, sham group, OVX group), Zuogui pill (6.4, 3.2, 1.6 g/kg) once a day and nilestriol (0.021 g/kg, positive group) once a week for 120 d. The right hind proximate femur at the 1/3 side were determined the bone mineral density (BMD) by dual-energy X-ray BMD absorptiometry. TGF-β1 and Smad4 mRNA expression of the left hind proximate femur at the 1/3 side were evaluated by the method of reverse transcriptase-PCR (RT-PCR). Results: As compared with the sham group, OVX group markedly decreased BMD (P<0.01). Zuogui pill (6.4, 3.2, 1.6 g/kg) and nilestriol group markedly promoted BMD compared with the model group (P<0.01). As compared with the sham group, OVX group markedly reduced TGF-β1 and Smad4 mRNA expression (P<0.01). Zuogui pill (6.4, 3.2, 1.6 g/kg) induced expression of TGF-β1 and Smad4 mRNA in bone tissue (P0.05). Conclusion: TGF-β1 and Smad4 mRNA expression were downregulated in bone tissue of model rats. Zuogui pill can prevent and cure postmenopausal osteoporosis (PMOP) effectively, one of the mechanisms may be related to its up-regulating the expression of TGF-β1/Smad4 mRNA in bone tissue.
Keywords: traditional Chinese medicine; Zuogui pill; postmenopausal osteoporosis; TGF-β1; Smad4
 
Title: Ultrasonic extraction technology optimization of polyphenols from Rhodiola rosea by response surface methodology
Authors: ZHAO Yan, ZHAO Tianqi, CAI Enbo, XU Yonghua, LIU Shuangli, YANG He, ZHANG Lianxue
PP: 2323-2330
Abstract: Objective: The paper studies extraction technique conditions of polyphenols from Rhodiola rosea. Methods: Using the response surface method (RSM), we optimize the extraction process of polyphenols from R.rosea. On the basis of single factor experiments, the ethanol concentration, ultrasonic extraction time and liquid-solid ratio are used as independent variables, polyphenols extraction rate as response value. According to the principle of center combination experiment designed with 3 factors and 5 levels of response surface analysis, interaction of each variable and its influence on the yield of polyphenols, simulation prediction model of quadratic polynomial regression equation was obtained. Results: After analyzing the significant interaction and the various factors, the optimum polyphenol extraction technology are as following: ethanol concentration 48% (volume ratio), ultrasonic extraction time 40 min, liquid-solid ratio 286 mL/g. Conclusion: Under the condition of optimum process, the polyphenols yield is 11.59%. The research model is suitable for polyphenols extraction from R.rosea, which can be used in practical production.
Keywords: Chinese medicine chemistry; Rhodiola rosea; polyphenols; response surface method
 
Title: Effect of Xixin and Fuzi on serum Na+-K+-ATPase and cAMP/cGMP with different proportions of Brady arrhythmia model rat with heart-kidney yang deficiency
Authors: ZHANG Mingfei, LIU Xinglong, JIA Bo, SHEN Tao, YOU Fengming, ZHANG Xiaodan, JIANG Cen
PP: 2317-2322
Abstract: Objective: To observe the effect of Xixin and Fuzi on Na+-K+-ATPase, cyclic guanine monophosphate (cGMP) and cyclic guanine monophosphate (cAMP) with different proportions on Brady arrhythmia(Ba)model rat with heart-kidney yang deficiency,and study the relationship of abnormal ion channels with Ba, and the intervene of Xixin with Fuzi on it. Methods: In this study, we used heart-kidney yang deficiency molding method together with intraperitoneal injection of adriamycin and hydrocortisone to Brady arrhythmia(Ba) rat model with heart-kidney yang deficiency. The model rats were treated by Xixin and Fuzi with different proportions, and the content of serum Na+-K+-ATPase, cGMP and cAMP were investigated by using enzyme linked immuno sorbent assay (ELISA). Results: Compared with blank control group, the serum content of cGMP increased significantly, but serum content of Na+-K+-ATPase, cAMP decreased significantly. After the intervene with Xixin and Fuzi, the group of Ⅳ(Xixin∶Fuzi=6∶12) could up-regulate the level of Na+-K+-ATPase and cAMP, and had obvious effect of down-regulating the level of cGMP. Conclusion: Xixin and Fuzi decoction can correct body ion channels in rat anomaly, improve myocardial cell contraction and play a role on curing Ba. Moreover, the effect of Ⅳ (Xixin∶Fuzi=6∶12) is the best.
Keywords: traditional Chinese pharmacology; Xixin and Fuzi; Brady arrhythmia model with heart-kidney yang deficiency; Na+-K+-ATPase; cyclic guanine monophosphate; cyclic adenosine monophosphate
 
Title: Research progress on the mechanism of inhibiting benign prostatic hyperplasia by traditional Chinese medicine
Authors: TAO Rui, MIAO Lin, FAN Guanwei, WANG Hong, WANG Yuefei, JIANG Xiaorui, CHAI Lijuan
PP: 2307-2316
Abstract: Benign prostatic hyperplasia (BPH) is a common disease with high incidence in elder men, while the main treatment of which is medication. Compared with the Western medicine, the traditional Chinese medicine (TCM) have not only shown their clinical effectiveness,but the advantages on their long-term use and less adverse effect have also been recognized. Several targets such as 5α-reductase, estrogen receptor, androgen receptor, and α-adrenergic receptor have been revealed that TCM played the important roles in the process of the monomers or compounds for BPH treatment. The mechanisms consists of the regulation of estrogen/androgen levels, their balance between estrogen and androgen, suppression of cell proliferation, down-regulation of growth factor expressions, induction of cell apoptosis, improvement of the lower urinary-tract symptoms condition. This review may provide developing more innovative, safe and effective medicine in future.
Keywords: traditional Chinese pharmacology; benign prostatic hyperplasia; review; estrogen; androgen