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June 15,2017
Volume 10,Issue 11
Pages -
Subject Area:Health Administration,Biomedical Engineering,Cardiovascular Diseases

Title: Analysis on the influencing factors of job satisfaction on new medical staff in township hospital

Authors: WANG Jinghui, ZHENG Zhinan, XIE Fengzhe, SHI Yu, ZHANG Shue, GOU Tianyu, FAN Chao, LI Li, LIU Xinyan, ZHANG Zhong, GAO Lei, SUN Tao
PP: 1281-1286
Abstract: Objective: To investigate the status of job satisfaction on new medical staff in township hospital and to discuss its influencing factors. Methods: Using the online survey method to collect the questionnaires, the descriptive statistics and Logistic regression analysis were used to analyze the influencing factors of job satisfaction. Results: Unit development prospects (Wald=5.765, P=0.016, OR=7.717), income (Wald=4.340, P=0.037, OR=1.965), social status (Wald=4.117, P=0.042, OR=1.853), realization of personal value (Wald=7.378, P=0.007, OR=2.218), family support (Wald=5.025, P=0.025, OR=1.895) had significantly positive prediction on job satisfaction (values of B are 2.043, 0.676, 0.617, 0.797, 0.639, P＜0.05). Conclusion: Unit development prospects, income, social status, realization of personal value and family support are all the protective factors of job satisfaction. Unit development prospects, social status and family support are the externally influencing factors of job satisfaction, while income and realization of personal value are the internally influencing factors of job satisfaction.
Keywords: health administration; township hospital; Logistic regression analysis; new medical staff; job satisfaction; influencing factors

Title: Research on key technology of photoelectric detection on colloidal gold detection card

Authors: FEI Wanru, HOU Lingling, YIN Shuyu, QI Yuan, CHENG Cheng, TAO Xiaojie, GONG Ping
PP: 1276-1280
Abstract: In order to improve the accuracy of colloidal gold detection, and reduce the subjective factors of the observer which leads to false negative and false positive results, a photoelectric detection method is proposed to semiquantitatively analyze the information of colloidal gold detection card. Hamamatsu S1133 is used for signal acquisition, and STM32F429 is used as the core processing unit. Combined with the position detection technology, a closed semiquantitative detection method of detected instrument and colloidal gold detection card are established. Compared with high performance liquid chromatography (HPLC) method, corresponding concentration of antigen in the sample and the detection signal should be achieved by calibrating the instrument, then rapid detection of colloidal gold detection card for high sensitivity is achieved. Through the experimental analysis, the accuracy of melamine detection can reach to ±12%, the lowest detection limits of clenbuterol, ractopamine and salbutamol are 3, 5, 10 ng/mL, respectively. Accuracy and detection level of colloidal gold detection are improved by using the method of photoelectric detection, which is suitable for rapid screening in the field.
Keywords: biomedical engineering; measurement technology and instruments; photoelectric detection; colloidal gold

Title: Experimental study on tea polyphenol compound oral liquid improving hematopoiesis and prolonging survival of irradiated mice

Authors: ZENG Yanan, ZHANG Meng, LAI Feng, CHENG Huiying, CHEN Qiu, CUI Fengmei
PP: 1268-1275
Abstract: Objective: To investigate the influence of tea polyphenol compound oral liquid on the routine blood test index and survival in mice after irradiation. Methods: The mice were randomly divided into 4 groups i.e. the normal control group, irradiation control group, tea polyphenol compound oral liquid low concentration group and high concentration group. The irradiation control and tea poly phenol compound oral liquid experimental groups were exposed 4 Gy 60Co γ-ray irradiation, and the mice of tea poly phenol compound oral liquid experimental groups were given tea polyphenol compound oral liquid for 7 d straight after radiation. The blood of the mice were obtained 7, 14, 21, 28 d after radiation, in which the content of white blood cells (WBC), lymphocytes (LYM), neutrophils (NEU), red blood cells (RBC), platelet (PLT), monocytes (MON), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean platelet volume (MPV) and plateletcrit (PCT) were detected. Meawhile, another part of mice received 8 Gy 60Co γ-ray irradiation and their survival condition was analyzed. Results: Tea polyphenol compound oral liquid can improve the number of peripheral WBC, LYM, NEU and PCT in mice after radiation, while it has no influence on RBC. Meanwhile, Tea polyphenol compound oral liquid has certain influence on the contents of MON, MCV, MCHC, and PCT in mice, but it has no influence on MCH and MPV, and it can also prolong survival time in mice after irradiation. Conclusion: Tea polyphenol compound oral liquid shows certain anti-radiation effect through protecting bone marrow hematopoietic function and it can prolong survival time as well in mice after γ-ray irradiation.
Keywords: radioactive medicine; tea polyphenol compound oral liquid; 60Co γ-ray; routine blood test

Title: Study on radiosensitivity of BMP11 on A549 cell

Authors: ZHAO Xiaolan, LI Susu, ZHOU Xinwen
PP: 1261-1267
Abstract: Objective: To explore the role of bone morphogenetic proteins 11 (BMP11) overexpression in the radiosensitivity of human non-small cell lung cancer A549 cell. Methods: The expression of BMP11 gene and protein was detected by Western blotting respectively. BMP11 gene expression vector was constructed by pcDNA3.1, and then was transferred into human non-small lung cancer A549 cells with lipofectamine. The experiments were divided into A549/BMP11 group, A549/NC group and blank control group. Radiosensitivity was evaluated by colony formation assay. The cell growth and proliferation were detected by using MTT kit. Flow cytometry was applied to analyze apoptosis. Results: BMP11 protein was expressed in cells, but the expression levels were different, and were involved in the regulation of radiosensitivity. Colony forming assay showed that the colony forming ability of the transfected group was significantly decreased compared with the control group, and the radiosensitivity of the combined treatment group was increased compared with the radiation group. MTT experiments showed that BMP11 significantly inhibited the growth of cells. Flow cytometry showed that the apoptosis rate of BMP11 transfected group was significantly higher than control group, P＜0.05. Conclusion: BMP11 is involved in the regulation of radiosensitivity, and can inhibit the increase of A549 cell by inducing apoptosis. BMP11 gene overexpression can increase the radiosensitivity of A549 cell, and its mechanism may be related to the induction of apoptosis.
Keywords: radioactive medicine; radiosensitivity; BMP11; A549; apoptosis

Title: Effects comparison between Schistosoma japonicum soluble worm antigen and soluble egg antigen on hepatic stellate cells (LX2)

Authors: WANG Lifu, YU Zilong, XIE Hui, WU Zhongdao, SUN Xi
PP: 1255-1260
Abstract: Objective: By stimulating LX2 cells with soluble worm antigen (SWA) and soluble egg antigen (SEA) and comparing the effects of SWA and SEA on LX2 cells, we investigated the mechanism of hepatic fibrosis when infected by Schistosoma japonicum. Methods: After treating LX2 cells with SWA, SWA+transforming growth factor-β (TGF-β), SEA, SEA+TGF-β, TGF-β, the LX2 cells activation related genes (α-SMA, Col1a1, CTGF, PPARγ, MMP9, MMP2, CCL2) were investigated by quantitative real-time PCR (qRT-PCR). Results: SWA treatment on LX2 cells results in the increases in the expression of α-SMA, Col1a1, CTGF and has synergistic effect with TGF-β, while treating with SEA results in the decreases in the expression of α-SMA, Col1a1, CTGF and increases in MMP9, CCL2. SEA can inhibit the effect of TGF-β on LX2 cells. Conclusion: SWA has the effects of promoting hepatic fibrosis and matrix remodelling, while SEA can inhibite hepatic fibrosis, matrix remodelling and regulate inflammatory mediator. These results provide theoretical basis on illustrating the development of hepatic fibrosis induced by S. japonicum infection.
Keywords: medical parasitology; Schistosoma japonicum; SWA; SEA; LX2 cells

Title: Benzyl isothiocyanate inhibits the growth of human lung cancer cells and induces protective autophagy

Authors: ZHANG Qicheng, PAN Zhenhua, LIU Boning, XU Ke
PP: 1244-1254
Abstract: Objective: Benzyl isothiocyanate (BITC) is a natural low-molecular compound with anti-tumor activity. However, the specific mechanism is still not fully understood. We investigated the growth inhibitory effect and autophagy induction of BITC in human lung cancer cells both in vitro and in vivo. Methods: Three kinds of human non-small cell lung cancer cell lines (A549, H661 and SK-MES-1) were tested. Cell growth inhibitory effect of BITC was determined by CCK-8 method. In BITC treated cells, autophagy induction was characterized by acridine orange (AO) staining, the processing of an autophagy marker protein: microtubule-associated protein 1 light chain 3 (LC3), and intracellular aggregation of LC3 into puncta structure. The specific role of induced autophagy in BITC treatment was studied by using autophagy inhibitors and siRNA. In vivo growth inhibition and autophagy induction were examined by A549 xenograft experiments. Results: The data showed that BITC could inhibit the growth of human lung cancer cells. Autophagy was induced in BITC-treated cells. Inhibition of autophagy by using 3-methyladenine (3-MA) and Atg5 knockdown could enhance the inhibitory effect. Xenograft experiments showed that BITC could inhibit the tumor growth and induced autophagy in cancer cells. Conclusion: BITC could inhibit the growth of human lung cancer cell both in vitro and in vivo and induces a protective autophagy response.
Keywords: molecular pharmacology; lung cancer; benzyl isothiocyanate; autophagy; anti-tumor activity

Title: Synthesis and anti-inflammatory activity of 9-O-cinnamicberberine

Authors: HE Fen, LIU Yanfei, ZHANG Shanshan, WANG Xiaohong, LI Yiqian, XIE Xiong, LIU Zhenbao
PP: 1237-1243
Abstract: 9-O-cinnamicberberine was synthesized from berberine and cinnamic acid via demethylation, acetylation and esterification, and its anti-inflammatory activity was evaluated using the xylene-induced ear swelling mice model. The ear swelling extent and the related inflammatory factors such as interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) in serum were observed. The structure of the target compound was confirmed by Fourier transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (1H NMR), 13C NMR and mass spectrum. The target compound has lowered the ear swelling extent and inhibited the serum levers of IL-6 and TNF-α in a dose dependent manner, which indicating this compound has anti-inflammatory activity.
Keywords: medicinal chemistry; berberine; 9-O-cinnamicberberine; pharmaceutical synthesis; anti-inflammatory activity

Title: Effect of different chlortetracycline salts on immune organ index and serum index of mouse

Authors: LIU Deng, WANG Jianhua
PP: 1231-1236
Abstract: Chlortetracycline hydrochloride (CTC∙HCl), chlortetracycline bisulfate (CTC∙H2SO4) and chlortetracycline mesylate (CTC∙CH3SO3H) were added daily to feed the mice as the treatment groups, and the control group did not receive any treatment. After raised 21 d and 42 d, the thymus index and spleen index were measured. The concentrations of CTC and the level of IgA, IgM and IgG in serum were also detected. The results are as followed: 1) Thymus indexes of the CTC∙H2SO4 and CTC∙CH3SO3H treatment groups were higher than control group (P＜0.05), but there was no significant difference between CTC∙HCl group and control group (P＞0.05). Thymus indexes of all groups were reduced at 42 d, and there was no clear difference on spleen index (P＞0.05). 2) The serum CTC concentrations of CTC∙H2SO4 and CTC∙CH3SO3H groups were significantly higher than CTC∙HCl group (P＜0.01). 3) The concentrations of IgA in CTC∙HCl, CTC∙H2SO4 and CTC∙CH3SO3H groups were lower than control group both at 21 d and 42 d (P＜0.05). The concentrations of IgM in treatment groups were lower than control group at 21 d (P＜0.01). Interestingly, the CTC∙H2SO4 and CTC∙CH3SO3H groups showed significant increase of IgM concentration compared with control group and CTC∙HCl group at 42 d consecutively (P＜0.01). As for IgG, all treatment groups had no significant difference with the control group at either 21 d or 42 d (P＞0.05). As a consequence, the CTC∙H2SO4 and CTC∙CH3SO3H have the potential to be used as feed supplement, and could increase the bioavailability of CTC in more animals.
Keywords: pharmacodynatics; chlortetracycline; immune organ index; serum index

Title: Delivery of anticancer nanomedicines: transport barriers and improvement strategies

Authors: WANG Qun, HE Zhonggui, ZHAO Dongyang
PP: 1223-1230
Abstract: This paper summarizes the factors which can influnence delivery process of anticancer nanomedicines, for example the abnormal angiogenesis, the size, shape and surface charge of nanomedicines. Then, the paper puts forward corresponding improvement strategies. It can promote the delivery of nanomedicines to tumor areas and then exert the anti-tumor effect by means of restoring angiogenesis and improving the properties of nanomedicines.
Keywords: pharmaceutics; anticancer nanomedicines; review; transport barriers; improvement strategies

Title: Clinical study on transcranial magnetoelectric encephalopathy treatment instrument for Alzheimer’s disease

Authors: TANG Qiang, ZOU Wei, SUN Zuodong, XING Yanli, YU Xueping, LI Kang, SUN Wuyi, WANG Wenhua, LIU Bo, ZHANG Li, DAI Xiaohong, HOU Yan
PP: 1216-1222
Abstract: Objective: To evaluate the treatment efficacy and safety for transcranial magnetoelectric (TME) encephalopathy treatment instrument (brand name: AOBO Alzheimer’s Treatment Instrument) for mild to moderate Alzheimer’s disease (AD). Methods: 80 patients with mild to moderate AD [Hachinski ischemia score≤4 cent, clinical dementia rating (CDR)=1.0 or CDR=2.0] were given double center, randomized, double blind, parallel and placebo controlled, clinical trial for 8 weeks, including treatment group and control group, each of 40 cases. All the patients were given regular basic medical treatment and standardized nursing care. The patients of treatment group were treated with TME encephalopathy treatment instrument, while the patients of control group were simulated with TME encephalopathy treatment instrument. Results: After treated for 8 weeks, compared with the control group, the treatment group’s scores of mini-mental state examination (MMSE), Alzheimer’s disease assessment scale (ADAS) and activities of daily living (ADL) improved significantly (differences between groups are P＜0.001, 0.000 1, 0.05, respectively). After treated for 4 weeks, the scores of MMSE and ADAS are improved (differences between groups are P＜0.05, 0.01, respectively). There was no adverse reaction in the two groups. Conclusion: TME encephalopathy treatment instrument has good therapeutic effect on mild to moderate AD patients, which can improve the mental state, cognitive behavior and self-care ability of daily life with safety.
Keywords: rehabilitation medicine; transcranial magnetoelectric; Alzheimer’s disease; voltage-gated Ca2+ channels; best target; theory of brain cell activation

Title: Expression of autophagy in aortic tissues in a mouse model of Marfan syndrome and its underlying mechanism

Authors: PENG Qunyong, ZHANG Saidan, ZHANG Wenxuan, PENG Zhenyu
PP: 1210-1215
Abstract: Objective: To investigate the expression of autophagy in aortic tissues in the established mouse model of Marfan syndrome and explore its mechanism. Methods: A total of 6 female wild-type C57BL/6J mice were divided into the control group and 6 female heterozygotes FBN1C1039G/+ mice were divided into the Marfan syndrome group. The mice were fed for 8 months and sacrificed. The aortic tissues were collected for further experiments. The pathological analysis was conducted by detecting aorta diameter and HE staining, the number of aortic autophagosomes was counted by transmission electron microscope (TEM), and the protein expressions of LC3-II and p62 were detected by Western blotting. Results: Both the diameter of aorta and the degree of elastic fiber rupture increased more significantly in the Marfan syndrome group than that of the control group. TEM results indicated that the number of autophagosomes was obviously decreased in the aorta smooth muscle cells of the Marfan syndrome group compared with the control group. In addition, the protein expression of LC3-II in the Marfan syndrome group was obviously decreased compared with the control group, while the protein expression of p62 was significantly increased in the Marfan syndrome group. Conclusion: The autophagy was significantly decreased in the aortic tissues of the Marfan syndrome group, which might contribute to decreasing the degradation of protein mediated by p62.
Keywords: cardiovascular diseases; Marfan syndrome; aortic aneurysm; smooth muscle cells; autophagy

Title: Correlation of CAT score and heart function in chronic obstructive pulmonary disease patients

Authors: ZHAO Xin, TU Youhui, DAI Mengyuan, JI Shuang, FEI Guanghe
PP: 1203-1209
Abstract: Objective: To investigate the correlation of chronic obstructive pulmonary disease (COPD) assessment test (CAT) score and the heart function in COPD patients. Methods: 138 patients diagnosed COPD were prospectively studied. Finally, 98 matches were recruited, including 48 mild-to-moderate COPD patients, 50 severe-to-very severe COPD patients, and 40 health subjects as normal control. Pulmonary artery pressure (PAP), the heart function parameters (RA-V, RVD, RVFW, LAD, LVD, TAPSE, LVEF, SV, Tei) were measured and recorded by color Doppler ultrasound. Pulmonary function, arterial blood gas were evaluated for each subject. Meanwhile, all subjects completed the CAT questionnaire independently. Results: Positive correlations were found between CAT scores and PAP (P＜0.01), CAT and the parameters of right ventricular function (RA-V, RVFW, RVD, Tei), while negative correlation was found between CAT and TAPSE (P＜0.05). No correlation was found between the CAT scores and the parameters of left ventricular function (LAD, LVD, LVEF, SV) (P＞0.05). Conclusion: The CAT scores of COPD patients were found significantly correlated with the parameters of right ventricular function, but not correlated with the parameters of left ventricular function, which indicates that CAT may also be used as a useful instrument to evaluate the severity of right heart function in COPD patients, so that can broaden the clinical application scope of the CAT.
Keywords: internal medicine; chronic obstructive pulmonary disease; CAT; heart function

Title: HGF attenuating the fibrosis of TGF-β1 by activating ERK signaling pathway

Authors: HAN Peilin, CUI Qingbo, FAN Meili, LU Wenjun, LI Zhou, LI Zhaozhu
PP: 1196-1202
Abstract: Objective: We explore the role of hepatocyte growth factor (HGF) in reducing transforming growth factor-β1 (TGF-β1)-induced fibrosis and the possible mechanism. Methods: The fibroblasts cultured in vitro were pretreated with different concentrations of HGF. The fibrosis induction of TGF-β1 was performed and the expression of fibronectin and α-smooth muscle actin (α-SMA) in the induced tendon fibroblasts and the phosphorylation of extracelluar regulated protein kinase (ERK) were detected. Followed by blocking the ERK signaling pathway, then we observed fibronectin and α-SMA expression. Results: HGF can attenuate the effect of TGF-β1 on fibrosis, and this neutralization is significantly attenuated when the ERK signaling pathway is blocked. Conclusion: This study confirms that HGF can reduce the fibrosis of TGF-β1 on tendon fibroblasts by ERK signaling pathway.
Keywords: surgery; hepatocyte growth factor; transforming growth factor-β1; fibrosis

Title: Research advance of tissue-engineered bone with vascularization

Authors: TELIEKE Kanzhale, YEERZHATI Hajiaheman, JIN Gele
PP: 1189-1195
Abstract: Construction of tissue-engineering bone (TEB) rapid vascularization is the main bottleneck and key factor of bone defect repair. The development of bone tissue engineering provides a promising way to repair large segments of bone defects. It has already showed tremendous potential for application on the basis of numerous animal experiments and rising clinical studies. However, osteogenesis of the TEB grafts may be unstable after TEB constructs implanted in vivo, especially in sites with poor blood supply for it cannot establish a blood supply connection with engine body in time. In recent years, the vascularization of TEB plays a vital role in generating large segments of bone grafts. In this paper, the review of the progress of the vascularization strategy suggests that the artificial bones cultured by bone tissue engineering can not only repair large areas of bone defects, but also be shaped and large prepared, and it is an ideal kind of bone repair materials. Vascularization TEB will be better applied in the treatment of patients with clinical bone defects.
Keywords: surgery; vascularization; review; tissue-engineered bone; cytokines; bone defect

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