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1. Compacted Sewage Sludge as a Barrier for Tailings: the Bacterial Community Structure Diversity in the Compacted Sludge | |||
ZHANG Qing,ZHANG Huyuan,WANG Jinfang | |||
Environmental Science and Techology 24 July 2015 | |||
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Abstract:Compacted sewage sludge barrier for tailings, known as reducing barrier, was innovatively double- functioned liner with its capsulation and elimination to Acid Mine Drainage (AMD) produced by tailings. The research had provided insights regarding the bacterial community structure diversity and dynamics over 75 days in the compacted sewage sludge specimens under 3 different seepage conditions (deionized water (DW), pH 2.1 sulfuric acid water (SA) and the synthetic AMD), by PCR-DGGE technique. The results indicated that at the start point (SP) of seepage, the 41st day (T1) of seepage or at the 75th day (T2) of seepage, under different seepage conditions (DW, SA and AMD), Clostridiales ,Bacillaceae and Carnobacteriaceae dominated in the compacted sewage sludge specimen with different relative abundance ranged from 46.26 % to 10.25 %. Redundancy analysis (RDA) on the influences between environmental factors (pH and heavy metals potential mobility in the compacted sludge specimens) and microbial community structure diversity showed the bacteria such as Carnobacteriaceae, Bacillaceae, Clostridiales Family XI. Incertae Sedis, Clostridiaceae, Lactobacillaceae, Peptoniphilaceae, Ruminococcaceae, Moraxellaceae, Caulobacteraceae, Corynebacteriaceae had positive relation to Zn potential mobility and pH; Carnobacteriaceae, Bacillaceae, Clostridiales Family XI. Incertae Sedis, Clostridiaceae, Micrococcineae, Moraxellaceae, uncertain family bacteria had positive relation with Fe potential mobility; Carnobacteriaceae, Clostridiaceae, Corynebacteriaceae had positive relation to Cd and Ni potential mobility; Ruminococcaceae had positive relation to Cu potential mobility. | |||
TO cite this article:ZHANG Qing,ZHANG Huyuan,WANG Jinfang. Compacted Sewage Sludge as a Barrier for Tailings: the Bacterial Community Structure Diversity in the Compacted Sludge[OL].[24 July 2015] http://en.paper.edu.cn/en_releasepaper/content/4650839 |
2. Indoor heating drives water bacterial growth and community metabolic profile changes in the building tap pipes in winter season | |||
ZHANG Haihan,CHEN Shengnan,BAI Yanru,MA Wenliang,LV Kai | |||
Environmental Science and Techology 11 May 2015 | |||
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Abstract:The growth of bacterial species harbored in the indoor drinking water taps was regulated by external environmental factors, and the people's health and drinking water safe was influenced by water quality of indoor drinking water taps. The indoor temperature increased significantly by indoor heating process. However, the mechanism of effect of indoor heating on bacterial re-growth from indoor drinking water taps was vastly not well understood. In the present work, flow cytometry and BIOLOG techniques were combined to explore the effects of indoor heating process on water bacterial cell concentrations and community metabolic profiles in building tap pipes. The results showed that the temperature of overnight stagnation water in the pipes was 15-17℃, after 10 min flushing of the indoor water taps, the water temperature decreased to 4-6℃. The highest bacterial cell number was observed in before flushing water (overnight stagnation), which is 5-11 times higher than that of flushed water. Meanwhile, the significant higher bacterial community metabolic activity (AWCD590nm) was also found in overnight stagnation water sample. The significant "flushed" and "taps" indicates that the AWCD590nm, and bacterial cell number varied among the taps within the flushed (P<0.01). Heatmap fingerprints and principle component analyses (PCA) revealed that a significant discrimination bacterial cell number and community functional metallic profiles in the overnight stagnation water and flushed water. Serine, Threonine, Glucose-Phosphat, Ketobutyric Acid, Phenylethyl-amine, Glycerol, Putrescine were significantly used by "Before" water samples. Our results suggested that the stagnation water with higher temperature should be discharged before composition. It is therefore that the data from this work can give more information on reasonable utilization of drinking water after stagnation in indoor pipes during the indoor heating process periods. | |||
TO cite this article:ZHANG Haihan,CHEN Shengnan,BAI Yanru, et al. Indoor heating drives water bacterial growth and community metabolic profile changes in the building tap pipes in winter season[OL].[11 May 2015] http://en.paper.edu.cn/en_releasepaper/content/4642162 |
3. Vertical Distribution of Bacterial Community Diversity and Water Quality during the Reservoir Thermal Stratification | |||
ZHANG Haihan,HUANG Tinglin,CHEN Shengnan,SHANG Panlu,BAI Yanru | |||
Environmental Science and Techology 27 April 2015 | |||
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Abstract:In this paper, water quality and bacterial community diversity was determined in a seasonal layered JIN PEN drinking water reservoir (Northwest China) using Illumina Miseq high-throughout sequencing targeting the V6 region of the 16S rRNA gene. Water samples were collected from 0.5, 5, 10, 20, 25, 30, 45, 50, 55 and 65 m depth of JIN PEN reservoir during thermal stratification. The results showed that epilimnion, metalimnion and hypolimnion were formed steadily in JIN PEN reservoir. The temperature was decreased steadily from the surface with 23.11℃ to the bottom with 9.17℃. Total nitrogen (TN) from 1.07 to 2.06 mg/L and nitrate nitrogen ranged from 0.8 to 1.84 mg/L. The dissolved oxygen (DO) concentration was decreased sharply below 50 m, and reached to zero in 65 m. The Miseq sequencing revealed a total of 4127 operational taxonomic units (OTUs) with 97% similarity, which were affiliated with 15 phyla including Acidobacteria, Actinobacteria, Armatimonadetes, Bacteroidetes, Caldiserica, Chlamydiae, Chlorobi, Chloroflexi, Cyanobacteria, Firmicutes, Gemmatimonadetes, Nitrospirae, Planctomycetes, Proteobacteria, and Verrucomicrobia. The highest Shannon diversity was 4.41 in 45 m, and the highest Chao 1 diversity was 506 in 5 m. Rhodobacter was dominated in 55 m (23.24%) and 65 m (12.58%). Prosthecobacter was dominated in 0.5-50 m. The heat map fingerprint and redundancy analysis (RDA) indicated significant difference in vertical water bacterial community composition in the reservoir. Meanwhile, water quality properties including dissolved oxygen, conductivity, nitrate nitrogen and total nitrogen have dramatically influence on the water bacterial community compositions. The data set yields new insights into the relationship between water quality and bacterial community compositions during reservoir thermal stratified periods. | |||
TO cite this article:ZHANG Haihan,HUANG Tinglin,CHEN Shengnan, et al. Vertical Distribution of Bacterial Community Diversity and Water Quality during the Reservoir Thermal Stratification[OL].[27 April 2015] http://en.paper.edu.cn/en_releasepaper/content/4640362 |
4. On Methodology for Ecological Risk Assessment of Agricultural Soil | |||
Zhiping Cao | |||
Environmental Science and Techology 20 January 2015 | |||
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Abstract:A few methodological issues were discussed in this paper, which involved in the ecological risk assessment in agricultural soil, including (1) Reference system: it was proposed to use forest soils in the mountainous areas as the reference systems. (2) The Distribution of research stations/sites: the research stations should be arranged according to the hierarchies of ecological factors. (3) Layout of quadrats in monitoring areas and sampling. (4) The indicators for ecological risk assessment: these indicators are designed on the basis of the biological mechanisms underlying the mineralization of soil organic matter. In reference system, the first, secondary, and tertiary hierarchies are climate zone, location, and site, respectively, the basic value of indicators in these different levels will be used for ecological risk assessment of agricultural soil. | |||
TO cite this article:Zhiping Cao. On Methodology for Ecological Risk Assessment of Agricultural Soil[OL].[20 January 2015] http://en.paper.edu.cn/en_releasepaper/content/4629791 |
5. Compacted Sewage Sludge as a Barrier for Tailings: the Microbial Functional Diversity in the Compacted Sludge Specimen | |||
ZHANG Qing,ZHANG Huyuan,WANG Jinfang,SUN Xun,YANG Bo | |||
Environmental Science and Techology 13 January 2015 | |||
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Abstract:he reducing barrier, using the compacted sewage sludge to be the liner for the tailings, made the liner have both encapsulating and eliminating functions. The microbial functional diversity ,heavy metal concentration, sulfate reducing bacteria (SRB) and total bacteria (TB) quantity in the compacted sludge with different seepage conditions (the deionized water(DW), the pH2.1 sulfuric acid and the simulate acid mine drainage(AMD)) were studied using Biolog and the Most Probable Number technique(MPN) testing system. The results showed that the average well color development (AWCD), the Shannon index (H), the Evenness index (E), the number of positive Ecoplate wells (S), the total bacteria (TB), and the sulfate reducing bacteria (SRB) quantity differed between different seepage conditions. There was no correlation between SRB quantity, Total bacteria quantity (TB) and the microbial community level functional diversity data. There was correlation between Cu concentration and Fe, Ni, Zn; between Fe and Ni. However, there was no correlation between Cd and other heavy metals. There was highly negative correlation between Cu, Fe, Ni concentration and the microbial community level functional diversity data. SRB quantity had correlation with Cd and Cu concentration. And TB quantity had correlation with Cu, Fe and Zn concentration. | |||
TO cite this article:ZHANG Qing,ZHANG Huyuan,WANG Jinfang, et al. Compacted Sewage Sludge as a Barrier for Tailings: the Microbial Functional Diversity in the Compacted Sludge Specimen[OL].[13 January 2015] http://en.paper.edu.cn/en_releasepaper/content/4626349 |
6. Comparion of MBR performance when Naproxen or Norfloxacin existed in sewage water | |||
Li Tang,Xue-jun Tan,Jiang-ping Qiu | |||
Environmental Science and Techology 16 December 2014 | |||
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Abstract:Anti-inflammatory agent (Naproxen) and antibiotics (Norfloxacin) were added into the sewage water separately to assess their effect on MBR performance. Membrane bioreactors show lower organic matters and nutrients removal when pharmaceuticals existed in treated water. Compared with traditional sewage water removal rate (COD was 89.84%, NH4+-N was 90.83%), the existence of Naproxen or Norfloxacin resulted in lower removal rate for COD (90.37% and 87.46%, respectively) and NH4+-N (70.12% and 73.68%,respectively). Undesirable settling property of active sludge was illustrated when adding Naproxen or Norfloxacin (even in low doses) into the test water. According to the TTC-ETS bioactivity test, Naproxen showed more poison effect than Norfloxacin, which was in accordance with the sludge sedimentation performance. Membrane fouling was illustrated by SEM and trans-membrane pressure analysis. The excess organic matter retained by membrane led to increasing membrane pollution. Critical pressure was reached about 30h ahead of normal time. | |||
TO cite this article:Li Tang,Xue-jun Tan,Jiang-ping Qiu. Comparion of MBR performance when Naproxen or Norfloxacin existed in sewage water[OL].[16 December 2014] http://en.paper.edu.cn/en_releasepaper/content/4623351 |
7. Cytotoxicity of silver nanoapticles to red blood cells: Size-dependent adsorption, uptake and hemolytic activity | |||
CHEN Liqiang,FANG Li | |||
Environmental Science and Techology 04 December 2014 | |||
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Abstract:In this paper, we examined the size-dependent nanotoxicity of AgNPs using three different characteristic sizes of 15 nm (AgNPs15), 50 nm (AgNPs50), and 100 nm (AgNPs100) against fish RBCs. Optical microscopy and TEM observations showed that AgNPs exhibited size effect on their adsorption and uptake by RBCs. The middle sized AgNPs50, compared with small or big ones, showed the highest level of adsorption and uptake by the RBCs, suggesting an optimal size of ~50 nm for passive uptake by RBCs. The toxic effects determined based on the hemolysis, membrane injury, lipid peroxidation and antioxidant enzymes production were fairly size and dose dependent. In particular, the smallest sized AgNPs15 had a greater ability to induce hemolysis and membrane damage than AgNPs50 and AgNPs100. Such cytotoxicity induced by AgNPs should attribute to the direct interaction of nanoparticle with the RBCs, resulting in the production of oxidative stress, membrane injury and subsequently hemolysis. Overall, the results suggest that particle size is a critical factor influencing the interaction between AgNPs and the RBCs. | |||
TO cite this article:CHEN Liqiang,FANG Li. Cytotoxicity of silver nanoapticles to red blood cells: Size-dependent adsorption, uptake and hemolytic activity[OL].[ 4 December 2014] http://en.paper.edu.cn/en_releasepaper/content/4621842 |
8. Comparison between continuous and intermittent ozonation for remediation of soils contaminated by polycyclic aromatic hydrocarbons | |||
Jiguo Wu,Jiang Yunxia,M. Prabhakar,Ye zhuoming,Yu Ruizhe,Zhou Hongwei | |||
Environmental Science and Techology 20 November 2014 | |||
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Abstract:Continuous ozonation can inactivate indigenous microbes due to the disinfection capability of ozone, which may affect subsequent bioremediation of soils. This study investigated the efficiency of removing polycyclic aromatic hydrocarbons from soils using intermittent ozonation technique, where ozone was sparged through the soil column every alternate day, resulting in shorter ozonation time for each ozonation circulation than continuous ozonation. The results showed that 85% Phe, 94% Ant, 76% Flu, 87% Pyr, and 91% BaP were removed on 32 d in continuous ozonation treatment, while 90% Phe, 84% Ant, 78% Flu, 81% Pyr, and 96% BaP were removed on 32 d in intermittent ozonation treatment, indicating both intermittent and continuous ozonation can effectively remove polycyclic aromatic hydrocarbons from soils. Fluorescien diacetate hydrolysis results indicated that the total microbial activity of intermittent ozonation was significantly (p < 0.05) higher than that of continuous ozonation treatment at 8, 16, 24 and 32 d. The toxicity bioassay of soil extracts showed that the relative luminescence increased from 5 to 30% at 8 d, without significant (p > 0.05) increase at 32 d in continuous ozonation treatment, while it increased to 61% at 32 d in intermittent ozonation treatment, indicating intermittent ozonation was more effective than continuous ozonation for the detoxification of soils contaminated with polycyclic aromatic hydrocarbons. It suggested that the both treatments were equally effective at removing polycyclic aromatic hydrocarbons from soil, but intermittent ozonation was better than continuous ozonaiton for further detoxification and maintaining the total microbial activity of soil. | |||
TO cite this article:Jiguo Wu,Jiang Yunxia,M. Prabhakar, et al. Comparison between continuous and intermittent ozonation for remediation of soils contaminated by polycyclic aromatic hydrocarbons[J]. |
9. Microbial enzym and biomass responses:deciphering vermifiltration treating excess sludge fed continuosly using Eisenia fetida despite seasonal variations | |||
XING Meiyan,Sun Wen,XU Zhe | |||
Environmental Science and Techology 26 August 2014 | |||
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Abstract:This paper reports on a seasonal pattern comparison of microbial enzymatic activities and biomass responses based on a conventional biofilter (BF, without earthworm) and a vermi?lter (VF, with earthworm, Eisenia fetida) for sewage sludge treatment fed continuously. The VSS reduction, viable cell number and enzyme activities were assayed to probe what made the VF operated stably. The results indicated that the earthworms' activities can further the volatile suspended solids (VSS) reduction with a 27.17% more than BF. Though the VF has a lower level in the viable cell number, the earthworms strongly improved the enzyme activities, such as INT- dehydrogenase, protease, β-glucosidase and amylase, which can explain the excellent performance of VSS reduction, compared with the BF. The correlation analysis documented that the VSS reduction was positively correlated with microbial enzyme activities. More importantly, the earthworm enabled the VF to avoid the detrimental influence of temperature, which guaranteed a stable performance during seasonal variations. | |||
TO cite this article:XING Meiyan,Sun Wen,XU Zhe. Microbial enzym and biomass responses:deciphering vermifiltration treating excess sludge fed continuosly using Eisenia fetida despite seasonal variations[OL].[26 August 2014] http://en.paper.edu.cn/en_releasepaper/content/4607574 |
10. The role of humic acid in stabilizing fullerene (C60) suspension | |||
ZHANG Luqing,ZHANG Yukun,LIN Xiuchun,YANG Kun,LIN Daohui | |||
Environmental Science and Techology 05 July 2014 | |||
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Abstract:Natural organic matter (NOM) can have a profound effect on the colloidal stability of discharged C60 nanoparticles in the water environment, which will influence the environmental behaviors and risks of C60 and therefore merits more specific studies. This study investigates the effects of humic acid (HA, as a model NOM) on the aqueous stabilization of C60 powders and on the colloidal stability of a previously suspended C60 suspension (aqu/nC60) as affected by variations of pH and ionic strength. Our results reveal that HA could disperse C60 powders in water to some degree, but was unable to stably suspend them. The aqu/nC60 could remain stable at pH > 4 but was destabilized at lower pH ratings. However, the colloidal stability of aqu/nC60 in the presence of HA was insensitive to pH from 3 to 11, owing to the HA adsorption on the nC60 and its increased electrosteric repulsions among the nC60 aggregates. The colloidal stability of aqu/nC60 with and without HA decreased as we increased the valence and concentration of the added cations. The HA was found to mitigate the destabilization effect of Na+ on the colloidal stability of aqu/nC60 by increasing the critical coagulation concentration (CCC) of Na+, while it lowered the CCCs of Ca2+ and La3+ probably owing to the bridging effect of nC60 with the HA aggregates formed through the intermolecular bridging of the HA macromolecules via cation complexation at high concentrations of cations with high valences. | |||
TO cite this article:ZHANG Luqing,ZHANG Yukun,LIN Xiuchun, et al. The role of humic acid in stabilizing fullerene (C60) suspension[J]. |
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