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1. Eco-physiological characteristics of three emergent macrophyte species to concentration changes of COD, nitrogen and phosphorus | |||
Sun Ruilian,Liu Jian | |||
Environmental Science and Techology 10 September 2013 | |||
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Abstract:Three macrophyte species (Zizania latifolia Turcz., Iris pseudacorus L. and Typha latifolia L.) were studied in constructed microcosm wetlands in order to compare the ability of hydrophytes in purifying simulated polluted river water and to analyze plant responses to oxidative stress induced by chemical oxygen demand (COD), nitrogen and phosphorus. The results showed that planted microcosms were more effective in removing COD, total nitrogen (TN) and total phosphorus (TP) than unplanted microcosms. Among the three hydrophytes, Z. latifolia was superior for TN removal, while I. pseudacorus units showed the highest TP removal efficiency. However, there was no significant difference between planted units in COD removal efficiencies. The plasticity of I. pseudacorus was the best exposed to sewage, which was associated with significantly higher SOD and CAT activities with treatment T1. However, treated with low COD/N/P (T1), the MDA levels of T. latifolia and Z. latifolia were basically unaffected. In addition, treatment of polluted river water induced enhancement in proline level of three species. It can be concluded that the antioxidative defence system and free proline accumulation were activated but could not resist the oxidative stress in plants exposed to high concentrations of COD/N/P (≥160/30/4 mg L-1). | |||
TO cite this article:Sun Ruilian,Liu Jian. Eco-physiological characteristics of three emergent macrophyte species to concentration changes of COD, nitrogen and phosphorus[OL].[10 September 2013] http://en.paper.edu.cn/en_releasepaper/content/4559293 |
2. Expression of anti-hyperglycaemic peptide MC6 from Momordica Chanrantia in Escherichia coli | |||
LIU Sixiu,SUI Guodong,FU Zhongping,MU Ruimin | |||
Environmental Science and Techology 12 January 2013 | |||
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Abstract:An anti-hyperglycaemic peptide MC6 [accession:AAX06814, B.nage et al, patent US 6852695] from Momordica Chanrantia with 18 amino acids was converted into nucleic acid sequence MC and synthesized according to E. coli prefer codons. Subsequently, MC was ligated to pET32a and expressed in E.coli. Recombinant protein pET-MC (about 21KD) was purified and analyzed by 12% SDS-PAGE. It showed that expressed recombinant pET-MC had good solubility in aqueous media. This result may provide such possibility that the bio-engineering bacteria methods can be used in the preparation of anti-diabetes medicines. | |||
TO cite this article:LIU Sixiu,SUI Guodong,FU Zhongping, et al. Expression of anti-hyperglycaemic peptide MC6 from Momordica Chanrantia in Escherichia coli[OL].[12 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4514714 |
3. The Influence of Adsorbed NOx Stability on NOx Trap Capacity below 200 ℃: Ceria-Zirconia Containing LNT Catalysts | |||
WANG Xinquan,LV Liangfang,ZHANG Qingqing,ZHANG Yuewei,WANG Jun,SHEN Meiqing | |||
Environmental Science and Techology 17 December 2012 | |||
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Abstract:The NOx trap capacity has been investigated by temperature programmed adsorption (TPA) experiments over a variety of Ce/Zr-containing catalysts. A fresh (F) or aged (A) Ce/Zr component, such as Ce0.58Zr0.42O2 (CZ64) or Ce0.80Zr0.20O2 (CZ82), was physical mixed with Pt/Al2O3 (PA) to form a Ce/Zr-Pt/Al2O3 mixture. PA-FCZ64, PA-FCZ82 and PA-ACZ64 show significant improvement in slowing down the premature NO release as temperature ramp from 100 to 150 ℃ However, an obvious NO release occurs on PA-ACZ82 under the same conditions. Using in situ DRIFT spectrometer, we found that nitrites oxidation by lattice oxygen of Ce/Zr was promoted on the fresh samples. It results in low coverage of nitrite with high stability. On the other hand, a large amount of nitrite was formed on PA-ACZ82, which could desorb upon increasing temperature. XPS results show a surface enrichment of Zr on the Ce/Zr samples, and the degree of the enrichment on CZ64 is higher than that on CZ82 for both fresh and aged samples. Segregation of CeO2 from the Ce/Zr phase have occurred on ACZ82, resulting in a lower stability of the nitrite species. | |||
TO cite this article:WANG Xinquan,LV Liangfang,ZHANG Qingqing, et al. The Influence of Adsorbed NOx Stability on NOx Trap Capacity below 200 ℃: Ceria-Zirconia Containing LNT Catalysts[OL].[17 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4500814 |
4. The influence of nonstoichiometry on LaMnO3 perovskite for catalytic NO oxidation | |||
CHEN Jiahao,SHEN Meiqing,WANG Xinquan,WANG Jun | |||
Environmental Science and Techology 11 December 2012 | |||
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Abstract:A series of structural modified LaxMnO3 (x = 0.9, 0.95, 1, 1.05, 1.11) perovskites used for NO oxidation was synthesized by a sol-gel method and characterized by XRD, BET, XPS, EPR and H2-TPR. La0.9MnO3 sample exhibits superior activity, and a 50% NO conversion at 250 C is obtained. XPS and EPR results reveal a higher Mn4+/Mn3+ ratio in Mn-rich samples, which induce more active oxygen bonding to Mn4+ due to the need for balancing the chemical states and stabilizing the structure. Easily regenerated oxygen associated with Mn4+ catalyzes the low-temperature NO oxidation. The constant activation energy (44.8 kJ/mol) obtained in the kinetics tests indicates the same mechanism of NO oxidation operated on all the samples. | |||
TO cite this article:CHEN Jiahao,SHEN Meiqing,WANG Xinquan, et al. The influence of nonstoichiometry on LaMnO3 perovskite for catalytic NO oxidation[OL].[11 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4500817 |
5. Preparation of polyethylenimine-functionalized graphene oxide composite and its application in electrochemical ammonia sensors | |||
SONG Min,XU Juan | |||
Environmental Science and Techology 23 November 2012 | |||
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Abstract:In this study, branched polyethyleneimine (PEI) was covalently linked to carboxylic acid functionalized graphene (GO-COOH) to form the GO-COOH/PEI composites. Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), Raman spectra and thermogravimetric analysis (TG) were used to characterize the obtained composites. Electrochemical measurements indicated that the modification of the composites on electrode could efficiently enhance the voltammetric response, suggesting the potential application for making electrochemical sensors. Moreover, our results also indicated that the electrocatalytic oxidation of ammonia could be observed on the GO-COOH/PEI composites modified glassy carbon electrode. Consequently, our observations demonstrated that GO-COOH/PEI composites were excellent materials for electrochemical sensing. | |||
TO cite this article:SONG Min,XU Juan. Preparation of polyethylenimine-functionalized graphene oxide composite and its application in electrochemical ammonia sensors[J]. |
6. Degradation of biphenyl and PCBs by isolated microorganism | |||
Qi Yun,Zhao Lin,Bao Shilong,Liang Wenjuan,Liu Shejiang | |||
Environmental Science and Techology 22 October 2012 | |||
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Abstract:Biphenyl is natural and synthetic aromatic compounds. This pollution has caused pubic concern due to its wide-spread use, improper handling, as well as its low degradability, strong bioaccumulation and high toxicity in the environment. Two bacterium strains, G-1 and G-2, were isolated from an activated sludge of a chemical factory wastewater treatment plant in China. Both the strains can use biphenyl as sole carbon and energy sources. The G-1 almost complete degraded it within 7 days, but can not utilized 3,3',4,4'-tetrachlorobiphenyl (PCB77) as sole carbon source. Although, train G-2 degrade biphenyl slower than G-1, it can utilize PCB 77. Strain G-2 was identified as Paracoccus according to phylogenetic similarity of 16S rRNA gene sequence. | |||
TO cite this article:Qi Yun,Zhao Lin,Bao Shilong, et al. Degradation of biphenyl and PCBs by isolated microorganism[OL].[22 October 2012] http://en.paper.edu.cn/en_releasepaper/content/4492400 |
7. A molecularly imprinted polymer for determining tributyltinchloride by LC-MS/MS | |||
ZHU Shanshan ,Futao HU,Ting YANG,Ning GAN | |||
Environmental Science and Techology 28 March 2012 | |||
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Abstract:There is a high interest in speciation of tributyltinchloride(TBT) in environment samples, such as seawater, due to the influence in the transmission of the contamination in the trophic chain. The environmental impact of organotin compounds has given particulate importance to analytical studies. This paper reports that applying a molecular imprinting technolgy with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to this field. The imprinted polymer has been synthesised by the non-covalent free redical approach using acrylamide(AM) as monomer in the presence of TBT as template molecule in acetonitrile solvent. The ability of the polymer synthesised to retain and distinguish TBT has been evaluated and optimized. The analytical characteristics included linearity(10-100000μgL-1), a pre-concentration factor of 100, and detection limit of 0.01 μgl-1. | |||
TO cite this article:ZHU Shanshan ,Futao HU,Ting YANG, et al. A molecularly imprinted polymer for determining tributyltinchloride by LC-MS/MS[OL].[28 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4472600 |
8. Study on the interaction locations between Humic acid and Pyrene | |||
MA Xingke,PENG Jinghe,ZHU Yaxian,WU Fang,ZHANG Yong | |||
Environmental Science and Techology 05 February 2012 | |||
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Abstract:IFluorescence quenching method was employed to determine the binding constant (Koc) of Humic acid (HA) - Pyrene (Py). The effects of pH value, ionic strength and heavy metal (HM) ions on the Koc were investigated and used to discuss the interaction mechanism between HA and Py. A fluorescent probe method was established and firstly applied to study the interaction between HA and Py by investigating the variation of I1/I3 with the chemical conditions of HA solution. Experimental results indicated that I1/I3 values showed an opposite trend compared to that of Koc, but an identical explanation on the interaction between HA and Py was obtained. The effects of HM ions on the interaction between HA and Py were dependent on the binding sites of HA and HM ions which was located at the carboxyl and hydroxyl. 1H NMR was applied to search for the binding site between HA and Py which was located at aliphatic chain of the HA.n this paper. | |||
TO cite this article:MA Xingke,PENG Jinghe,ZHU Yaxian, et al. Study on the interaction locations between Humic acid and Pyrene[OL].[ 5 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4465128 |
9. Effects of 2,6-dinitrotoluene exposure on gene expression in the liver of common carp (Cyprinus carpio) | |||
Xu Jingbo,Gao Chao | |||
Environmental Science and Techology 02 January 2012 | |||
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Abstract:In order to characterize the effect of 2, 6-dinitrotoluene (2, 6-DNT) on gene expression in the liver of common carp (Cyprinus carpio), fish was exposed via water for 15 days at concentrations of 0.195 and 1.95 mg/l 2,6-DNT in semi-static bioassay. Liver was selected as target tissue. Custom microarrays were constructed from cDNA libraries obtained with Suppression Subtractive Hybridization-Polymerase Chain Reaction (SSH-PCR) experiments. Microarray data revealed that the expression of several genes in the liver was influenced by 2,6-DNT exposure and real-time PCR was used to confirm these gene expression changes. The affected genes were mainly involved in energy metabolism, biotransformation and stress response. The pattern of EROD, GST,Na+/K+-ATPase, and GK enzyme activity in liver of common carp may have a close relationship with the gene expression and stress response to 2, 6-DNT. Furthermore, the RCF, HSI, and the available glycogen reserves of the exposed fish were significantly lower after 15 days of exposure than in the control fish. Through principal component analysis, effects by 2, 6-DNT-stress in each test group were distinguished. In this study, indications of a trade-off between the metabolic cost of toxicant exposure and processes vital to the survival of the organism were seen on the molecular level as well as on higher levels of biological organization. | |||
TO cite this article:Xu Jingbo,Gao Chao. Effects of 2,6-dinitrotoluene exposure on gene expression in the liver of common carp (Cyprinus carpio)[OL].[ 2 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4459282 |
10. Effect of carbon tetrachloride stress on antioxidative performance of wheat seedlings | |||
Tao Honglin,Shan Aiqin | |||
Environmental Science and Techology 10 July 2011 | |||
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Abstract:To assess the CT toxicity to plants in soil, the antioxidative performance and the level of lipid peroxidation in wheat seedlings under CT stress were investigated by simulated experiments. The results indicated that: 1) The trend of activity of POD and SOD went to conformity, which were activated by CT ranging from 0.32 to 1.60 mgokg-1 and were inhibited by CT above 1.60 mgokg-1. 2) Under CT stress, the antioxidative performance of wheat seedlings was firstly activated within a short-term period, and then inhibited. 3) The lipid peroxidation of wheat seedlings wasn't obvious at CT concentration lower than 0.32 mgokg-1. But when CT concentration ranging from 0.32 to 1.60 mgokg-1, the MDA content increased obviously and the plasmalemma lipid could not be protected from peroxiding by activated POD and SOD. 4) At medium and late stage, the POD and SOD activity had a decreasing trend with increasing CT concentration, while the MDA content had significant positive correlation (with 99% confidence interval) with CT concentration. | |||
TO cite this article:Tao Honglin,Shan Aiqin. Effect of carbon tetrachloride stress on antioxidative performance of wheat seedlings[OL].[10 July 2011] http://en.paper.edu.cn/en_releasepaper/content/4435158 |
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