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1. Identification of thidiazuron-induced ESTs expressed differentially during callus differentiation of alfalfa (Medicago sativa L. cv. Jinnan) | |||
Zhang Chun-Rong ,Huang Xue-Lin ,Wu Jian-Yong ,Feng Bi-Hong,Chen Yunfeng | |||
Biology 05 December 2005 | |||
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Abstract:Embryogenic callus induced from petioles of alfalfa (Medicago sativa L. cv. Jinnan) and maintained on kinetin (KN)-containing B5h medium becomes non-embryogenic with differentiation of tracheary elements when kinetin in the medium is replaced by thidiazuron (TDZ). To investigate the molecular mechanisms underlying the TDZ-induced morphological competence transition, we cloned the differentially expressed sequence tags (ESTs) from TDZ-treated callus of alfalfa using suppression subtractive hybridization (SSH). The expression patterns of genes induced by TDZ were found to change throughout the process of TDZ treatment. A homeodomain-leucine zipper protein gene, MSHB1, which was induced by TDZ, may be a transcription factor in the TDZ-regulation of alfalfa callus differentiation. The induction of trehalose-6-phosphate phosphatase (TPP), which may decrease the level of trehalose-6-phosphate, and the induction of 1-aminocyclopropane-1-carboxylate synthase (ACS), which increases the level of ethylene, by TDZ may be responsible for the termination of somatic embryogenesis. | |||
TO cite this article:Zhang Chun-Rong ,Huang Xue-Lin ,Wu Jian-Yong , et al. Identification of thidiazuron-induced ESTs expressed differentially during callus differentiation of alfalfa (Medicago sativa L. cv. Jinnan)[OL].[ 5 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4069 |
2. Comparative studies of senescence-related enzymes in the cotyledon of chlorophyll b–deficient mutant and its wild type oilseed rape during senescence | |||
Tingting Qin,Jingfeng Fu,Nianhui Zhang,Linfang Du | |||
Biology 28 November 2005 | |||
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Abstract:The change patterns of senescence-related enzymes during cotyledon senescence were studied in a chlorophyll (Chl) b-deficient mutant type (MT) and its wild type (WT) of Brassica napus L. seedlings. The fresh weight on the basis of cotyledon number initially increased till 20 days after planting (DAP) and then kept relative constant. The protein content decreased sharply since 20 DAP and Chl content reduced since 10 DAP in both two types; however the rate of degradation in protein and Chl in the MT was slower than that in the WT since 20 DAP. Superoxide dismutase (SOD; E.C.1.15.1.1) activity declined in the WT but increased in the MT since 20 DAP. Activity of peroxidase (POD; E.C.1.11.1.7) increased markedly after 20 DAP in both types. Esterase (EST; E.C.3.1.1.1) activity increased in both two types since 10 DAP, whereas at 40 DAP it was much lower in the MT than that in the WT. In addition, bands patterns of SOD, POD and EST isozymes were changed during cotyledon development in both two types, but some differences were observed. Cu/ZnSODs activities were higher in the MT at 40 DAP as compared with the WT. These results showed that day 20 was the turning point during the cotyledon development and the senescence in Cr3529 cotyledon was slower than that in the WT. | |||
TO cite this article:Tingting Qin,Jingfeng Fu,Nianhui Zhang, et al. Comparative studies of senescence-related enzymes in the cotyledon of chlorophyll b–deficient mutant and its wild type oilseed rape during senescence[OL].[28 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3804 |
3. Cloning and analysis of a cytochrome P450 gene from rice | |||
Zhu Shifeng,Cao Xuesong,Zhong Yongwang ,Wei Chunhong,Li Yi | |||
Biology 25 November 2005 | |||
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Abstract:Cytochrome P450 mono-oxygenases (P450s) are a superfamily of heme-dependent enzymes which play important roles in almost all of organisms. Based on rice genome database, a gene encoding a rice cytochrome P450 mono-oxygenase was cloned and sequenced (GenBank accession no. AY579214). The coding region of the gene was 1530 nucleotides in length and encoding a protein with 510 amino acid residues in length. Sequence analyses indicated that the cytochrome P450 corresponds to an ent-kaurene oxidase (KO) like protein, which is likely to be involved in phytoanticipins/phytoalexins biosynthesis. Southern blots analysis suggested that there are at least four similar genes encoding ent-kaurene oxidase or ent-kaurene oxidase like proteins in rice genome. Therefore, the gene reported in this paper was named OsKOS1. RT-PCR analysis showed that OsKOS1 was expressed in vegetative organs and reproductive organs, but at a higher level in vegetative organs. The expression level of OsKOS1 in RDV (rice dwarf virus)-infected plants was much lower than that in healthy plants, indicating that OsKOS1 is likely to be involved in defending RDV. | |||
TO cite this article:Zhu Shifeng,Cao Xuesong,Zhong Yongwang , et al. Cloning and analysis of a cytochrome P450 gene from rice[OL].[25 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3792 |
4. Effect of gibberellic acid and abscisic acid pretreatment on seedling growth and α-amylase activity in endosperms of wheat | |||
Xiangru Liao,Qun Sun,Xuejun Li,Junfeng Gao | |||
Biology 04 November 2005 | |||
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Abstract:eeds of Triticum aestivum L. cv. Zhengyin No1 were treated by 10 μmol/L GA3 and 10 μmol /L ABA before germination respectively. The growth of root and shoot, contents of endosperm amylose and soluble carbohydrate, medial pH value, the utilizing efficiency of storage materials (MUE), and activity of α-amylase in endosperm during seed germination under osmotic stress were investigated. The results showed that shoot growth was promoted by GA3 and inhibited by ABA and osmotic stress. Root growth was promoted by GA3, and retarded by osmotic stress and ABA at early stage. But the growth of root of seedlings from seeds treated by ABA was promoted significantly during the late germination stage. Seedling growth was positively correlated with medial pH and MUE significantly. GA3 promoted the expression and increase in activity of α-amylase in endosperm of wheat seed, resulting in increase in breakdown of amylose to soluble carbohydrates, but ABA and osmotic stress had the opposite effect to GA | |||
TO cite this article:Xiangru Liao,Qun Sun,Xuejun Li, et al. Effect of gibberellic acid and abscisic acid pretreatment on seedling growth and α-amylase activity in endosperms of wheat[OL].[ 4 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3504 |
5. Effect of silver nitrate on callus regeneration and activity of some enzymes during tissue culture of wheat immature inflorescence | |||
Xiangru Liao,Lei Tang,Feng Zhang | |||
Biology 01 November 2005 | |||
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Abstract:Effects of silver nitrate on callus regeneration and activity of indole-3-acetic acid oxidase and peroxidase, glutathione S-transferase and reductase, aldehyde oxidase, catalase and peroxidase in cells of immature inflorescence of Triticum aestivum L. variety JI135 were investigated. Immature inflorescences with length between 5-10mm were treated with AgNO3 in MS medium in a period of 10 days. The results indicated that callus regeneration was improved by treatment of wheat immature inflorescence with 16 mg/L AgNO3 for 7 days. The activity of indole-3-acetic acid oxidase and peroxidase was reduced obviously by 16 mg/L AgNO3 four days and six days after inoculation respectively, activity of glutathione transferase, glutathione reductase,catalase,and peroxidase were obviously promoted by AgNO3 4 days after the treatment, But changes in activity of the aldehyde oxidase was not significantly influenced by AgNO3. The mechanism of AgNO3 in improving cell regeneration was discussed. | |||
TO cite this article:Xiangru Liao,Lei Tang,Feng Zhang. Effect of silver nitrate on callus regeneration and activity of some enzymes during tissue culture of wheat immature inflorescence[OL].[ 1 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3441 |
6. Evidence of High Sensitivity of Stomatal development for Gene Mutations by Comparing Experiment in Some Mutants of Arabidopsis | |||
Zhuxia Shen,Genxuan Wang,Hao Zhang ,Muqing Qiu,Xingzheng Zhao | |||
Biology 01 November 2005 | |||
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Abstract:e sought to gain insight into the gene regulation mechanism of stomatal development and investigate the relationship among some different genes. The nail enamel printing was conducted to observe the stomatal indices of some mutants including eins, cins, abis, era1, gca2, CaMs and phr1, analyse the stomatal indices among them by ANOVA. In the wild type and 16 mutants, the stomatal index did not differ with respect to location on the lower epidermis. When compared with wt, the stomatal indices of 16 mutants changed significantly, the change of mutant eins was most obvious, stomatal index in insensitive and enhanced ABA mutants and the enhance or intervened expression of CaM mutants, the index all decrease. The stomata intensity was highly sensitive to most mutations in genome, which could be used as an index of the relative regulation in the genome transcription signal and metabolic webs. We hypothesis that stomatal development is control by multigene, these gene interact with each othe | |||
TO cite this article:Zhuxia Shen,Genxuan Wang,Hao Zhang , et al. Evidence of High Sensitivity of Stomatal development for Gene Mutations by Comparing Experiment in Some Mutants of Arabidopsis[OL].[ 1 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3440 |
7. A novel cytochrome p450 monooxygenase CYP81A6 encoded by rice Bel locus confers resistance to two different classes of herbicides | |||
Pan Gang,Zhang Xianyin ,Liu Kede ,Zhang Jiwen ,Wu Xiaozhi,Tu Jumin ,Zhu Jun | |||
Biology 05 September 2005 | |||
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Abstract:he present studies described characterization of two rice bentazon-lethal mutants, bel1 and bel2, which were created via γ-ray radiation in an indica photo-thermo-sensitive genic male sterile (P/TGMS) line, W6154S, and a japonica inbred line, Norin8, respectively. Both mutants of bel1 and bel2 started to show affected symptoms at a concentration of 100 mg/L and became lethal at 300 mg/L bentazon but within this range bel1 is slightly more tolerance than bel2. The lowest concentrations of their bentazon-sensitivity, however, were about 120-fold lower than those of their wild controls. Using bel1 to cross with bel2 followed by herbicide test of their progenies, we confirmed that these two mutants were genetically allele each other. The data from PCR amplification followed by sequence comparisons between wild and mutant alleles revealed a single base-pair deletion in each mutant and for bel1 this occurred at 408th bp in its second exon, while for bel2 this at 507th bp in its first exon. | |||
TO cite this article:Pan Gang,Zhang Xianyin ,Liu Kede , et al. A novel cytochrome p450 monooxygenase CYP81A6 encoded by rice Bel locus confers resistance to two different classes of herbicides[OL].[ 5 September 2005] http://en.paper.edu.cn/en_releasepaper/content/2785 |
8. Complex antimicrobial agent Ag-carboxymethyl chitosan-thiabendazole: Structure, properties and application in water-soluble coatings | |||
Xia Jinlan,Wang Chun,Nie Zhenyuan,Peng An’an,Guan Xin | |||
Biology 29 April 2005 | |||
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Abstract:he structure, properties, and application in water-soluble coatings of a new complex antimicrobial agent Ag-carboxylmethyl citosan-thiabendazole (Ag-CMCTS-TBZ) prepared from different materiel ratios were reported. The silver ions were preferably coordinated with the free –NH2 groups and the –OH groups of secondary alcohol and carboxyl in CMCTS. TBZ preferably bonded to carboxyl group in CMCTS by electrostatic force and hydrogen bonding. Increase in content of silver ions in the complex agent improved in some limited extent the antibacterial activity, but enhanced coloring and cost of the complex agent. Increase in TBZ content resulted in increase of antifungal activity, but decrease of water solubility of the complex agent. The antimicrobial MIC of the complex agent to Esherichia coli, Staphylococcus aureus, Candida albicans, Aspergillus niger, Mucor sp. were 20~80, 15~60, 20~55, 40~250, and 400~1700 mg/kg, respectively. Addition of 0.1% of this complex agent to acrylic emulsion pain | |||
TO cite this article:Xia Jinlan,Wang Chun,Nie Zhenyuan, et al. Complex antimicrobial agent Ag-carboxymethyl chitosan-thiabendazole: Structure, properties and application in water-soluble coatings[OL].[29 April 2005] http://en.paper.edu.cn/en_releasepaper/content/1964 |
9. Preparation, optical properties and cell staining of water soluble amine-terminated PAMAM G2.0-Au nanocomposites | |||
Xia Jinlan,Fu Jindian,Nie Zhenyuan,Shen Li | |||
Biology 27 April 2005 | |||
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Abstract:he solution chemical and optical characteristics of formation of NH2-PAMAM G2.0-Au nanocomposites in the aqueous solution of amine-terminated polyamidoamine dendrimer G2.0 (NH2-PAMAM G2.0) under various mole ratios of Au(III)/PAMAM were studied by both UV-Vis spectrometry and fluorospectrometry. The NH2-PAMAM G2.0-Au nanocomposites, with a type of structure in which one Au nanoparticle was surrounded by several NH2-PAMAM dendrimers, emitted strong bluish violet fluorescence, and were uniform, water soluble and biocompatible as well as very stable in frozen conditions. The sizes of gold nanoparticles in the nanocomposites were about 2.5nm and decreased with increase of NH2-PAMAM G2.0 concentration. The NH2-PAMAM G2.0 played an important role in being as host or micro-reactor for Au(Ⅲ) before Au(Ⅲ) reduction and being as dispersant and stabilizer for gold nanoparticles after Au(Ⅲ) reduction. Preliminary experiments of cells staining to human embryonic lung fibroblast (HELF) cell lines s | |||
TO cite this article:Xia Jinlan,Fu Jindian,Nie Zhenyuan, et al. Preparation, optical properties and cell staining of water soluble amine-terminated PAMAM G2.0-Au nanocomposites[OL].[27 April 2005] http://en.paper.edu.cn/en_releasepaper/content/1949 |
10. Isolation and Purification of ATP Synthase in Mitochondria | |||
zhujie,sunrunguang | |||
Biology 19 April 2005 | |||
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Abstract:In the past 50 years, scientists have done so much on ATP synthase’s structure and its function already, but people known function more and the mechanism fewer, and there are much more work need to do. High purified ATPase and subunits which should come firstly are the key factors in study of enzyme’s structure and catalytic mechanism. This review has summarized some valuable methods in extraction and purification of ATP synthase through compared with kinds of methods came from different workgroups all of the world , and mainly introduced the isolation of bovine heart mitochondria, submichondrial particles, F1-ATPase, F0-ATPase and their subunits such as a,b ,g,d , e in F1-ATPase and b, c, d, e, f, g, h, F6, A6L in F0 moiety and oligomycin sensitive conferring ATPase (OSCP) and the ATPase inhibitor (IF1) in details. | |||
TO cite this article:zhujie,sunrunguang. Isolation and Purification of ATP Synthase in Mitochondria[OL].[19 April 2005] http://en.paper.edu.cn/en_releasepaper/content/1886 |
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