Chinese︱Feedback︱Save this page

Login︱Register

• Elaborating Academic Views 　　　　 • Exchanging Innovative Ideas
• Protecting Intellectual Properties 　　• Fast Sharing Science Papers

Sponsored by the Center for Science and Technology Development of the Ministry of Education
Supervised by Ministry of Education of the People's Republic of China

Home > Papers > Cell-Biology

There are 39 papers published in subject: > since this site started.

Results per page:

39 Total, 4 Pages | << First < Previous 1 2 3 4

Select Subject

All (8828)

Agronomy (158)

Animal Husbandry,... (64)

Aquatic Science (16)

Astronomy (36)

Aviation (22)

Basic Medicine (184)

Biology (504)

Chemical Engineering (103)

Chemistry (635)

Civil and Archite... (105)

Clinical Medicine (236)

Computer Science ... (924)

Dynamic and Elect... (121)

Earth Science (119)

Economics (67)

Education (31)

Electrics, Commun... (927)

Energy Science an... (67)

Engineering and T... (15)

Environmental Sci... (140)

Food Science and ... (63)

Forestry (36)

Hydraulic Enginee... (40)

Mechanics (240)

Metallurgical Eng... (32)

Mine Engineering ... (38)

Nuclear Science a... (21)

Pharmacy (98)

Physics (920)

Preventive Medici... (37)

Psychology (20)

Security Science ... (17)

Sports Science (6)

Surveying and Map... (15)

Textile Science a... (16)

Traditional Chine... (56)

Transportation En... (57)

Third-level Subject

Cell Biophysics(0)
Cell Physiology(2)
Cell Structure and Morpho...(0)
Cellular Evolution(0)
Cellular Immunology(6)
Cytopathology(0)
Other Subjects of Cell Bi...(2)

Rank

Results per page:

39 Total, 4 Pages | << First < Previous 1 2 3 4

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.


	1. Using Mammospheres as a Model to Determine Protein Signatures Presented in Breast Cancer Stem Cells
	guifa li,yuxiangjiang,dongchen,wiwang,yukuncui,fukunzhao
	Biology 23 February 2009
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:The breast cancer stem cells have been proposed to underpin many types of breast Cancer. also,mammospheres have been widely employed to study breast cancer stem cells. In current study, we have used the approaches of Proteomics to study the altered protein expression in mammospheres from the most commonly used breast cancer cell line MCF-7. we have identified 34 differentially expressed proteins in Mammospheres from parental cells. Of these 34 proteins, only seven expressed higher in Mammosheres. We find that FKBP4 and GIPC1 which is high associated with insulin-like growth factor I receptor was low expressed in mammospheres. The two proteins and their implications in breast cancer drug resistance are currently under study in our laboratory.
	TO cite this article:guifa li,yuxiangjiang,dongchen, et al. Using Mammospheres as a Model to Determine Protein Signatures Presented in Breast Cancer Stem Cells[OL].[23 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29434


	2. A Filament-Assembling-Pressure Model for Developing Forces by Actin Polymerizing
	Wang Xiaoen
	Biology 13 February 2009
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:The investigations of mechanisms for developing forces by actin polymerization come mainly from two models so far, sliding filament and Brownian ratchet models. Both models were difficult to clarify many experiment phenomena, and made some confusion at theory. Such mechanism is still not full understood because of difficulty in experiment observations at molecular level. Based on nucleation mechanisms of crystal, Young’s pressure, and ability of filaments to transmit water, this paper proposes a filament-assembling-pressure model by which can explain almost all of interrelated experiment phenomena. Assembly of a filament at its one end will produce redundant water because of monomer disappearance in the small microcosmic circumscription about polymerization site. The redundant water will diffuse to other end along the filament, and result in a pressure of water flow, filament-assembling pressure, by which propels loads, transfers signal or nutriments, and transforms chemical energy of polymerization into forces.
	TO cite this article:Wang Xiaoen. A Filament-Assembling-Pressure Model for Developing Forces by Actin Polymerizing[OL].[13 February 2009] http://en.paper.edu.cn/en_releasepaper/content/28983

Saved Papers

Saved Papers


	3. High-frequency somatic embryogenesis and plant regeneration with high content of gentiopicroside from the Chinese medicinal plant, Gentiana straminea Maxim
	Yunfei Cai, YanLing Liu,Yanfei Wang,Fengning Xiang,Guangmin Xia
	Biology 13 January 2009
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Gentiana straminea Maxim. (MahuaQinjiao) is an important Chinese medicinal herb that is rich in secoiridoid. To increase the production of this plant, we have developed an efficient tissue culture system for high-frequency plant regeneration. We have also obtained plants with high secoiridoid monomer production through tissue culture and somatic embryogenesis. We have found that MB medium is better than B5 medium for callus induction. Calli induced from immature seeds are superior to those from hypocotyls or young leaves in their ability to regenerate via somatic embryogenesis. We have also demonstrated that 2,4-dichlorophenoxyacetic acid is efficient for both callus induction and embryogenesis, indole-3-acetic acid is suitable for embryogenic callus proliferation and N6-(benzyl)-adenine promotes both embryo development and the accumulation of gentiopicroside in the cultures. Regenerated plants have been selected to for high gentiopicroside content. One plant contains 5.82% of gentiopicroside, which is higher than the control plant (1.20-3.73%). The regenerated plants are genetically more stable than the calli based on both cytological and RAPD analyses.
	TO cite this article:Yunfei Cai, YanLing Liu,Yanfei Wang, et al. High-frequency somatic embryogenesis and plant regeneration with high content of gentiopicroside from the Chinese medicinal plant, Gentiana straminea Maxim[OL].[13 January 2009] http://en.paper.edu.cn/en_releasepaper/content/27702


	4. Allicin-Induced Apoptosis in EL-4 Murine T-lytuphocytes in Vitro
	Zhiheng Cao ,Ziren Wang
	Biology 02 February 2008
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Objective: The present study was undertaken to evaluate anti-proliferative and pro-Apoptotic activities of allicin—a plant derived organics sulfur compounds (OSC) in murine T-lymphocytes (EL-4) and try to determine its molecular mechanism of inducing apoptosis. Methods: Trypan blue dye exclusion method was used to determine cells concentration, the apoptotic rate and morphologic alteration of nuclei were measured by Hoechst 33258 fluorescent dye. DNA spallation was determined by using TUNEL kit. Protein expression of Bax, Bcl-2, cythrome C, Caspase-3, -12 were detected by Immunohistochemistry (IHC). Fluorescent microscope was used to determine the change of mitchrodria membrane potential (MMP). Result: Allicin is effective in inhibiting the proliferation of EL-4 cells in vitro in concentration-dependent manner, induces the formation of apoptotic bodies, nuclear condendensation, DNA spallation, and it even activates the expression of caspase-3, -12, cytochrome C, upregulates the ratio of the heterodimer Bax-Bcl-2 and induces MMP decrease. Conclusions: Allicin may induce apioptosis in EL-4 cells with a time- and concentration-dependent manner, in which the mitochnodria pathway may play a central role.
	TO cite this article:Zhiheng Cao ,Ziren Wang. Allicin-Induced Apoptosis in EL-4 Murine T-lytuphocytes in Vitro[OL].[ 2 February 2008] http://en.paper.edu.cn/en_releasepaper/content/18573


	5. EFFECTS OF GLYCEROL PRETREATMENT ON RECOVERY AND ANTIOXIDANT ENZYME ACTIVITIES OF LYOPHILIZED RED BLOOD CELLS
	Zhou Xinli ,He Hui ,Liu Baolin ,Hua ZeChao ,Chen Ying
	Biology 29 September 2007
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Successful storage of red blood cells (RBCs) by freeze-drying technique has important implications in blood transfusion and clinical medicine. We presented a method of preservation RBCs by pretreating them with glycerol solution and then freeze-drying. The effects of glycerol pretreatment on recovery and antioxidant enzyme activities of lyophilized RBCs were investigated. RBCs pretreated in 40% glycerol resulted in 55.3±4.26% numerical recovery and 53.5±3.85% hemoglobin recovery, which were significantly different from control group (P<0.01). Superoxide dismutase(SOD), catalase (CAT) and glutathione peroxidase (GSH-PX) activities of 40% glycerol pretreated, freeze-dried RBCs almost remained fully active after freeze-drying. After 180 days storage, they decreased by 25.6%，21.5% and 18.4%, respectively, which are significantly lower than those of no glycerol pretreated, freeze-dried RBCs (P<0.1). These data demonstrated that glycerol pretreatment had beneficial effects on the recovery and antioxidant enzyme activities of lyophilized RBCs.
	TO cite this article:Zhou Xinli ,He Hui ,Liu Baolin , et al. EFFECTS OF GLYCEROL PRETREATMENT ON RECOVERY AND ANTIOXIDANT ENZYME ACTIVITIES OF LYOPHILIZED RED BLOOD CELLS[OL].[29 September 2007] http://en.paper.edu.cn/en_releasepaper/content/15435


	6. Primary study of a Novel Protein F46-crp That Contributes to Centrosome Duplication
	Wei Yi,Shen Enzhi,Fan Jinling,Liu Qian,Jan Marc,Wang Yongchao,Sun Le,Liang Qianjin
	Biology 09 March 2007
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Using a serum from a patient suffering from an autoimmune disease (progressive systemic sclerosis), a novel centrosome related protein F46-crp was detected. We identified the target protein by immunoprecipitation and Western blot and tandem mass spectrometry sequencing. The results show the protein F46-crp has an apparent molecular mass of about 60 kDa. A cDNA of HeLa F46-crp protein was obtained using RT-PCR, and a recombinant F46-crp used to generate monoclonal antibodies. Immunofluorescence microscopy of synchronized HeLa cells labeled with the anti-F46-crp -specific antibodies revealed that F46-crp localized exclusively to the centrosome during interphase, although it completely disappeared at the onset of mitosis. After effectively silencing the F46-crp by antisense RNA, cell growth and proliferation were strongly inhibited and the cell cycle typically stalled at S phase. The silencing also resulted in the formation of poly-centrosomal and multinucleate cells, which finally became apoptotic. These results suggest that F46-crp is a novel centrosome related protein, whose expression is cell cycle-dependent, and it is involved in centrosome duplication.
	TO cite this article:Wei Yi,Shen Enzhi,Fan Jinling, et al. Primary study of a Novel Protein F46-crp That Contributes to Centrosome Duplication [OL].[ 9 March 2007] http://en.paper.edu.cn/en_releasepaper/content/11354


	7. Molecular cloning, characterization and expression analysis of an Interleukin-2 enhancer binding factor 2 homologue from Tetraodon nigroviridis
	Xiang Lixin ,Shao Jianzhong
	Biology 09 November 2006
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Interleukin-2 enhancer binding factor 2 (ILF2) was reported to regulate transcription of interleukin-2 (IL-2), a central cytokine in the regulation of T-cell responses. This property of ILF2 was well characterized in human and mammals, but little is known in bony fish. In this paper, an ILF2 homologue was cloned and well characterized from Tetraodon nigroviridi for the further investigation of the function of ILF2 in bony fish. The full-length Tetraodon ILF2 cDNA was 1380 bp in size and contained an open reading frame (ORF) of 1164 bp that translates into a 387 amino-acid peptide with a molecular weight of 42.9 kDa, a 5’ untranslated region (UTR) of 57 bp, and a 3’ UTR of 159 bp containing a poly A tail. The deduced peptide of Tetraodon ILF2 shared an overall identity of 58%~93% with other known ILF2 sequences, and contained two N-glycosylation sites, two N-myristoylation sites, one RGD cell attachment sequence, six protein kinase C phosphorylation sites, one amino-terminal RGG-rich single-stranded RNA-binding domain, and a DZF zinc-finger nucleic acid binding domain, most of which were highly conserved through species compared. Constitutive expression of Tetraodon ILF2 was observed in all tissues examined, including gill, gut, head kidney, spleen, liver, brain and heart. The highest expression was detected in heart, followed by liver, head kidney and brain. Stimulation with LPS did not significantly alter the expression of Tetraodon ILF2. Gene organization analysis showed that the Tetraodon ILF2 gene have fifteen exons, one more than other known ILF2 genes in human and mouse. Genes up- and down-stream from the Tetraodon ILF2 were Rpa12, Peroxin-11b, Smad4, Snapap and Txnip homologue, which were different from that in human and mouse.
	TO cite this article:Xiang Lixin ,Shao Jianzhong. Molecular cloning, characterization and expression analysis of an Interleukin-2 enhancer binding factor 2 homologue from Tetraodon nigroviridis[OL].[ 9 November 2006] http://en.paper.edu.cn/en_releasepaper/content/9393


	8. Suppressing Phosphatidylcholine-specific Phospholipase C and elevating ROS level, NADPH oxidase activity and Rb level induced neuronal differentiation in mesenchymal stem cells
	Wang Nan ,Xie Kun ,Huo Siwei ,Zhao Jing,Zhang Shangli,Miao Junying
	Biology 18 October 2006
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:In the previous research, we found that D609 (tricyclodecan-9-yl-xanthogenate) could induce human marrow stromal cell (hMSC) differentiation to neuron-like cells. In this study, to understand the possible mechanism, we sequentially investigated the changes of phosphatidylcholine-pecific phospholipase C (PC-PLC) activity, the expression of Rb, the intracellular reactive oxygen species (ROS) levels, NADPH oxidase and superoxide dismutase (SOD) activities when D609 induced neuronal differentiation in rat mesenchymal stem cells (MSCs). The results showed that D609 obviously inhibited the activity of PC-PLC when it induced neuronal differentiation in rat MSCs. Simultaneously, ROS level and the activity of NADPH oxidase increased significantly, but the MnSOD and Cu/ZnSOD activities were not altered. Furthermore, the level of Rb protein was evidently elevated. Our data suggested that PC-PLC mediated neuronal differentiation of rat MSCs by elevating NADPH oxidase activity, ROS level, and up-regulating the expression of Rb protein.
	TO cite this article:Wang Nan ,Xie Kun ,Huo Siwei , et al. Suppressing Phosphatidylcholine-specific Phospholipase C and elevating ROS level, NADPH oxidase activity and Rb level induced neuronal differentiation in mesenchymal stem cells[OL].[18 October 2006] http://en.paper.edu.cn/en_releasepaper/content/8811


	9. Antisense Inhibition of Rubisco Activase Increases Rubisco Content and Alters the Proportion of Rubisco Activase in Stoma and Thylakoids in Chloroplasts of Rice Leaves
	Songheng Jin,Jian Hong,Xueqin Li,Dean Jiang
	Biology 30 December 2005
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubsico) activase (RCA) is a nuclear-encoded chloroplast protein that modifies the conformation of Rubisco, releases inhibitors from the active sites, and increases enzymatic activity. It appears to have other functions, which are related to its distribution within the chloroplast. The aim of this research was to resolve uncertainty about the localization of RCA, and to determine whether the distributions of Rubisco and RCA were altered when RCA content was reduced. Gas exchange and Rubisco were measured, and the sub-cellular locations of Rubisco and RCA were determined using immunogold-labeling electron microscopy, in wild-type and antisense rca rice plants. Net photosynthetic rate and the initial Rubisco activity in the antisense rca plants decreased much less than RCA content in the antisense plants. Immunocytolocalization showed that Rubisco in wild-type and antisense plants was localized in the stroma of chloroplasts. However, the amount of Rubisco in the antisense rca plants was greater than in the wild type plants. RCA was detected in both the stroma and in the thylakoid membranes of wild-type plants. We show that the percentage of RCA labeling in the thylakoid membrane was substantially decreased, while the fraction in the stroma was increased, by the antisense rca treatment. From the changes in RCA distribution and alterations in Rubisco activity, RCA in stroma of chloroplast probably contributes to the activation of Rubisco, and RCA in thylakoids compensates for the reduction of RCA in the stroma, allowing steady-state photosynthesis to be maintained when RCA is depleted. RCA may also have a second role in protecting membranes against environmental stresses as a chaperone.
	TO cite this article:Songheng Jin,Jian Hong,Xueqin Li, et al. Antisense Inhibition of Rubisco Activase Increases Rubisco Content and Alters the Proportion of Rubisco Activase in Stoma and Thylakoids in Chloroplasts of Rice Leaves[OL].[30 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4775

	Check out RSS, or use RSS reader to subscribe this item