Authentication email has already been sent, please check your email box: and activate it as soon as possible.
You can login to My Profile and manage your email alerts.
If you haven’t received the email, please:
|
|
There are 78 papers published in subject: > since this site started. |
Select Subject |
Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
1. In Silico Conserved Motif and Promoter Analysis of the MIKCc-type MADS-Box Genes in Tomato | |||
Chen Hongyu,Chen Xiuling,Li Jingfu,Wang Aoxue | |||
Biology 09 January 2013 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:In plants, MADS-box genes have been shown to be involved in all aspects of plant development, especially in flower development in angiosperms such as Arabidopsis. To understand the diversity in regulation present in these subfamilies, we investigated 42 MIKCc-type MADS-box genes from the genome of tomato, including 7 genes that have not previously been characterized. Using these sequences, we have performed a comparative analysis of tomato MIKCc-type MADS-box genes, including the description of the protein conserved motif, promoter motif and genome localization of each member. Our results suggest that many genes have diverged in their sequences after duplication and/or speciation. They also suggest that these genes may have evolved through segmental duplication events produced by polyploidy events in the past. Overall, the comparative analysis of the tomato MIKCc-type MADS-box genes will be expected to result in significant progress in investigating the regulation mechanism of MADS-box genes in higher plants. | |||
TO cite this article:Chen Hongyu,Chen Xiuling,Li Jingfu, et al. In Silico Conserved Motif and Promoter Analysis of the MIKCc-type MADS-Box Genes in Tomato[OL].[ 9 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4512884 |
2. Sequence Variants on Chromosomes 20q13.33, 11q23.3 and 5p15.33 are Associated with Glioma Susceptibility in a Chinese Population | |||
Chen Hongyan,Zhao Yao,Chen Yuanyuan,Daru Lu | |||
Biology 05 January 2013 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Two glioma genome-wide association studies in European populations identified 14 genetic variants strongly associated with risk of glioma, but it is unknown whether these variants associate with glioma risk in Asian populations. The authors genotyped these 14 variants in 976 glioma patients and 1,057 control subjects to evaluate their associations with risk of glioma, particularly high-grade glioma glioblastoma (n=312), in a Chinese population. Overall, we identified three susceptibility loci on 20q13.33 RTEL1 rs6010620 (P=2.79×10?6), 11q23.3 PHLDB1 rs498872 (P=3.8×10?6) and 5p15.33 TERT rs2736100 (P=0.0002) for glioma risk in this study population; also for glioblastoma risk on20q13.33 RTEL1 rs6010620 (P=3.57×10?7), 11q23.3 PHLDB1 rs498872 (P=3.8×10?6), and 5p15.33 TERT rs2736100 and rs2736098 (P=1.21×10?4, 2.84×10?4, respectively). This study provides further evidence of the three glioma susceptibility regions in 20q13.33, 11q23.3 and 5p15.33 in Chinese populations. | |||
TO cite this article:Chen Hongyan,Zhao Yao,Chen Yuanyuan, et al. Sequence Variants on Chromosomes 20q13.33, 11q23.3 and 5p15.33 are Associated with Glioma Susceptibility in a Chinese Population[OL].[ 5 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4510854 |
3. Inhibition of hepatitis B virus (HBV) by LNA-mediated nuclear interference with HBV DNA transcription | |||
Sun Zhen,Chen Hongyan,Lu Daru | |||
Biology 04 January 2013 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Silencing target genes with small regulatory RNAs is widely used to investigate gene function and therapeutic drug development. Recently,triplex-based approaches have provided another attractive means to achieve targeted gene regulation and gene manipulation at the molecular and cellular levels. Nuclear entry of oligonucleotides and enhancement of their af?nity to the DNA targets are key points of such approaches. In this study, we developed lipid-based transport of a locked-nucleic-acid (LNA)-modi?ed oligonucleotide for hepatitis B virus (HBV) DNA interference in human hepatocytes expressing HBV genomic DNA. In these cells, the LNA-modi?ed oligonucleotides passed ef?ciently across the cell membrane, and lipid-coating facilitated translocation from the cytoplasm to the nucleus. The oligonucleotide specifically targeting HBV DNA clearly interfered with HBV DNA transcription as shown by a block in pregenomic RNA (pgRNA) production. The HBV DNA-targeted oligonucleotide suppressed HBV DNA replication and HBV protein production more ef?ciently than small interfering RNAs directed to the pgRNA. These results demonstrate that fusion with lipid can carry LNA-modi?ed oligonucleotides to the nucleus where they regulate gene expression. Interfering with HBV DNA transcription by LNA-mod-i?ed oligonucleotides has strong potential as a new strategy for HBV inhibition. | |||
TO cite this article:Sun Zhen,Chen Hongyan,Lu Daru. Inhibition of hepatitis B virus (HBV) by LNA-mediated nuclear interference with HBV DNA transcription[OL].[ 4 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4511934 |
4. Interplay of Sl-miR164 and NO APICAL MERISTEM gene control flower morphogenesis | |||
Yang Chunwen,Li Zhengguo | |||
Biology 24 May 2012 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:MicroRNAs (miRNAs), which are about 21 nucleotide RNAs, are ubiquitous regulators of gene expression in eukaryotic organisms. Some miRNAs act as an important endogenous regulator in plant organ formation and development. miR164 is a conservative miRNA family that exists in a variety of different plants. Studies showed that miR164 regulates lateral root formation and leaf shape. However, there is no any report about miR164 involved in flower initiation and fruit development. We analyze the genomic DNA in tomato (Solanum lycopersicum) and find only one ortholog, designated as Sl-miR164. The target gene (NAM) of Sl-miR164 was predicted and cloned. Quantitative RT-PCR shows miR164 expresses highly in stem and fruit, whereas Nam expresses highly in all tissues, including root, stem, leaf, flower and fruit. Overexpression of miR164 results in delayed petal withering and falling off, and overexpression of NAM gene results in abnomal flower with thick sepal, no petal and stamen. The transgenic plants,overexpressing miR164 and NAM gene result in parthenocarpic fruit. Moreover, Sl-miR164 and Sl-NAM overexpression altered the transcript levels of a number of MADS box transcription factor genes, which involved in flower development and morphology. Our data demonstrate that tomato Sl-miR164 play important role in flower morphogenesis and fruit development. | |||
TO cite this article:Yang Chunwen,Li Zhengguo. Interplay of Sl-miR164 and NO APICAL MERISTEM gene control flower morphogenesis[OL].[24 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4479634 |
5. Cloning and Functional characterization of three Superoxide Dismutases genes from halophyte Salicornia europaea and Thellungiella halophila | |||
Wu Fan ,Mei Yu,Lu Maolong,Juan Li,Wang Rongfu,Wang Xianlei,Sun Qizhen,Jie Zang,Kai Xu,Hu Ji | |||
Biology 17 April 2012 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:In order to study the halophyte salt-tolerance mechanism, we cloned the manganese (Mn) and Cu/Zn superoxide dismutase (SOD) full-length cDNAs from Salicornia europaea by RACE method for the first time, sequence analysis indicated that the MnSOD gene (GenBank accession number: JQ061158) comprises an open reading frame of 699 bp, encoding 233-amino acid polypeptide with a predicted molecular mass of 25.7 kDa. Correspondingly, the Cu/ZnSOD gene (GenBank accession number: JQ061160) consists of an open reading frame of 684 bp which encodes a protein of 228 amino acids with a predicted molecular mass of 23.3 kDa. The prokaryotic expression vectors pET30-SeMSD, pET30-SeCSD and pET30-ThMSD were constructed, and the target proteins were expressed successfully in BL21 Escherichia coli. Through optimization of the inducing concentration of Isopropyl β-D-Thiogalactopyranoside (IPTG), we tested the salt tolerance of these three superoxide dismutases under 6.5% and 7.5% NaCl, and the results demonstrate that the recombinants BL21 (pET30-SeMSD) and BL21 (pET30-ThMSD) show better tolerance to salinity stress in comparison with the control stain BL21 (pET30), but the recombinant BL21 (pET30-SeCSD) has not displayed increased salt tolerance. | |||
TO cite this article:Wu Fan ,Mei Yu,Lu Maolong, et al. Cloning and Functional characterization of three Superoxide Dismutases genes from halophyte Salicornia europaea and Thellungiella halophila[OL].[17 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4474413 |
6. The adenylate cyclase gene MaAC is required for virulence and multi-stress tolerance of Metarhizium acridum | |||
Liu Shuyang ,Guoxiong Peng,Yuxian Xia | |||
Biology 22 March 2012 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Background: The efficacy of entomopathogenic fungi in pest control is mainly affected by various adverse environmental factors, such as heat shock and UV-B radiation, and by responses of the host insect, such as oxidative stress, osmotic stress and fever. In this study, an adenylate cyclase gene (MaAC) was cloned from the locust-specific entomopathogenic fungus Metarhizium acridum, which is homologous to various fungal adenylate cyclase genes. RNA silencing was adapted to analyze the role of MaAC in virulence and tolerance to adverse factors from environment and host insect. Results: Compared with the wild type, the vegetative growth of the RNAi mutant was decreased in PD (potato dextrose medium), Czapek-dox and PDA plates, respectively, the cAMP levels was also reduced in PD liquid culture. Knockdown of MaAC by RNAi led to a great reduction in fungal growth in the hemolymph of locusts after injection and topical inoculation, thus demonstrating that MaAC encodes an adenylate cyclase and is required for virulence of M. acridum. Virulence assay indicated that the effect of MaAC on the virulence was mainly inside the host locust. A plate assay indicated that the tolerances of the MaAC RNAi mutant under oxidative stress, osmotic stress, heat shock and UV-B radiation was decreased compared with the wild type. Conclusion: MaAC affects virulence, primarily by fungal growth inside the insect, and is required for tolerance to oxidative stress, osmotic stress, heat shock and UV-B radiation. MaAC affects the fungal virulence via vegetative growth and tolerance against oxidative stress, osmotic stress and locust fever. | |||
TO cite this article:Liu Shuyang ,Guoxiong Peng,Yuxian Xia. The adenylate cyclase gene MaAC is required for virulence and multi-stress tolerance of Metarhizium acridum[OL].[22 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4471763 |
7. The binding and unwinding properties of the Bloom helicase catalytic core to the G4DNA structure | |||
LUO Heng,XU Houqiang,CAI Mingjuan,CHEN Xiang,DING Mei,LI Kun,MENG Huihui | |||
Biology 13 March 2012 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:G4DNA, which widely exists in the structure of the telomeres in normal cells, plays a pivotal part in the process of prolonging the telomere DNA by catalyzing the enzyme telomerase. Bloom (BLM) helicase, an important member of the RecQ DNA helicase family, plays an important role in DNA metabolism, including DNA replication, repair, transcription, and recombination. The unwinding of G4DNA requires DNA helicase participation, which is crucial for maintaining chromosomal stability. This study was conducted to determine the DNA-binding and unwinding properties of the BLM helicase catalytic core to G4DNA using fluorescence polarization and the electrophoretic mobility shift assay (EMSA). The results revealed that the BLM helicase catalytic core could bind and unwind G4DNA. The molecular affinity of G4DNA binding by the helicase was dependent on the single-stranded DNA (ssDNA) terminals in the G4DNA; the helicase binds to the G4DNA where one helicase monomer covers approximately 10 nucleotides at the 3' or 5' ssDNA tail. The unwinding of G4DNA was dependent on the presence of a 3' ssDNA tail and ATP; the G4DNA with only a 3' ssDNA tail was the most favorable substrate to be unwound by the BLM helicase catalytic core, and required 3' ssDNA tails of at least 10 nt in length for efficient unwinding. The blunt-ended G4DNA was loosely bound and partly unwound by the helicase catalytic core. The experimental results presented are beneficial to further the understanding the functionality of BLM helicase in cells. | |||
TO cite this article:LUO Heng,XU Houqiang,CAI Mingjuan, et al. The binding and unwinding properties of the Bloom helicase catalytic core to the G4DNA structure[OL].[13 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4471423 |
8. ac109 is required for the nucleocapsid assembly of Autographa californica multiple nucleopolyhedrovirus | |||
Lin Lin,Riqiang Deng,Jinwen Wang,Jianhao Ke,Hongkai Wu,Xunzhang Wang | |||
Biology 12 March 2012 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:ORF109 (Ac109) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a highly conserved gene in all sequenced baculovirus genomes, but its function is not known. This paper describes generation of an ac109 knockout virus (Ac-ac109-KO-GP) and analyses of the influence of ac109 deletion on the virus replication in Sf-9 cells so as to investigate the role of ac109 in the viral life cycle. Results revealed that budded virus (BV) yields and occlusion body synthesis were completely blocked in cells infected with the mutant virus. Electron microscopy demonstrated that ac109 deletion blocked nucleocapsid formation, though infection was initiated and electron-dense bodies associated with the virogenic stroma appeared. The mutant phenotype was rescued by an ac109 rescue virus. On the other hand, real-time PCR analysis indicated that ac109 is not required for viral DNA replication. Thus, these results suggested that ac109 plays an important role in AcMNPV nucleocapsid formation. | |||
TO cite this article:Lin Lin,Riqiang Deng,Jinwen Wang, et al. ac109 is required for the nucleocapsid assembly of Autographa californica multiple nucleopolyhedrovirus[OL].[12 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4471364 |
9. Comparative transcriptome analysis between insect-resistant cotton and non-transgenic cotton (Gossypium hirsutum L.) in response to salt stress | |||
Fangjiao Xu,Mingliang Jia,Junli Feng,Ying Liang,Yanjun Liang,Jishuang Chen | |||
Biology 12 March 2012 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Insect-resistant cotton is one of the main transgenic crops in the world. High salinity immensely limits insect-resistant cotton growth and productivity. To determined genes in response to salt stress and clarify reasons of transcription level changes caused by insect-resistant factor, gene expression microarray was used to monitor the differentially expressed genes in insect-resistant cotton CCRI 41 and non-transgenic cotton CCRI 23. Microarray analysis showed that 2341 genes were up-regulated and 3073genes were repressed in CCRI 41 exposed for 24 h to 200 mM NaCl. A total of 7674 salt-resistant genes of CCRI 23 were identified, the expression levels of 2341 genes were enhanced, and the transcript levels of 4718 genes were decreased by 200 mM NaCl. Gene ontology of annotated genes revealed that some of the salt responsive genes involved to important processes as the response to osmotic stress, abscisic acid stimulus and reactive oxygen species were induced by the NaCl treatment. Comparative transcriptome analysis between CCRI 41and CCRI 23 in response to salt stress exhibited that toxin catabolic process and regulation strategy of DNA level played important role in response to salt stress. Quantitative RT-PCR was used to validate 4 selected salt responsive genes. This work represents the first study in disclosing the possible molecular evidence that insect-resistant cotton have weak tolerance against NaCl stress. Comparative transcriptome analysis will assist in laying a foundation for cultivating of insect-resistant and salt-resistant cotton. | |||
TO cite this article:Fangjiao Xu,Mingliang Jia,Junli Feng, et al. Comparative transcriptome analysis between insect-resistant cotton and non-transgenic cotton (Gossypium hirsutum L.) in response to salt stress[OL].[12 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4471217 |
10. Bioinformatics and expression analysis of the amino acid transporter gene family (OsAAT) in rice | |||
CAI Hongmei | |||
Biology 09 March 2012 | |||
Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
Abstract:Nitrogen (N) is one of the most important limiting factors for plant growth and development. Amino acids are the major source of organic N, which is converted from inorganic N absorbed by plant roots from the soil. Amino acid transporters are the principal mediators of organic N distribution and important regulators of resource allocation in plants. Although the complete genomic sequence of rice has already been released, there is still little known about amino acid transporter genes in rice. In this study, 79 OsAAT genes were identified by a database search of the rice genome based upon HMM profiles. A bioinformatics analysis of the complete set of OsAAT genes is presented, including chromosomal location, phylogenetic analysis, gene structure, protein analysis, conserved motifs, protein structures and cis-element analysis of the promoters. In addition, the comprehensive expression profile of OsAAT genes in rice tissues/organs under N starvation conditions was investigated by real-time PCR analysis. Diverse expression patterns of OsAAT genes indicated diverse biological functions of the amino acid transporters and the important roles of OsAAT genes in N uptake, metabolism and distribution during N starvation. | |||
TO cite this article:CAI Hongmei. Bioinformatics and expression analysis of the amino acid transporter gene family (OsAAT) in rice[OL].[ 9 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4470810 |
Select/Unselect all | For Selected Papers |
Saved Papers
Please enter a name for this paper to be shown in your personalized Saved Papers list
|
About Sciencepaper Online | Privacy Policy | Terms & Conditions | Contact Us
© 2003-2012 Sciencepaper Online. unless otherwise stated