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| There are 37 papers published in subject: > since this site started. | |||
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| 1. Isolation and characterization of ten novel polymorphic microsatellites loci in the Chinese pond turtle (Mauremys reevesii) and cross-species amplification in other Cryptodira species | |||
| Liu Luo,Nie Liuwang,Bu Xinjiang,Xia Xingquan,Huang Zhenfeng,Jing Wanxing,Jiang Yuan,Wang Ling | |||
| Biology 22 February 2012 | |||
| Show/Hide Abstract | Cite this paper︱Full-text: PDF (0 B) | |||
| Abstract: Ten polymorphic microsatellite loci were developed for the Chinese Pond turtle (Mauremys reevesii) from (AC)n and (GATA)n microsatellite-enriched genomic library. We screened 32 wild individuals from the middle and lower reaches of Changjiang River and detected high levels of polymorphism for all ten loci. The number of alleles/locus ranging from 3 to 18 with the values of expected and observed heterozygosities ranging from 0.585 to 0.907 and 0.469 to 1.000, respectively. Cross-species amplification of these microsatellite loci was also tested in additional seven freshwater turtle/half freshwater turtle species (Mauremys megalocephala, Cuora galbinifrons, Cuora flavomarginata, Mauremys mutica, Mauremys sinensis, Pelochelys cantorii, Rafetus swinhoei) of Cryptodira. All 10 loci were ampli?ed speciffc products in the five species of Geoemydidae, while in the P. cantorii and the R. swinhoei of Trionychidae only one locus and two loci worked, respectively. Therefore, these highly variable loci may serve as a valuable tool for population genetics analyses, gene flow and provide information on the evolutionary history of the species. | |||
| TO cite this article:Liu Luo,Nie Liuwang,Bu Xinjiang, et al. Isolation and characterization of ten novel polymorphic microsatellites loci in the Chinese pond turtle (Mauremys reevesii) and cross-species amplification in other Cryptodira species[OL].[22 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4468139 | |||
| 2. Assessment of alternative cropping systems in terms of economic benefit, water use and soil conservation: a case study in the semi-arid Loess Plateau, China | |||
| Jia Yu,Hu Bin,Zhao Zhi-hong,Li Feng-min,Wang Xiao-ling,Kadambot H.M.Siddique | |||
Biology 06 January 2012
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| Abstract:Water and wind erosion are main degradation processes in drylands around the world. In the arid/semi-arid regions, erosion is accentuated by fragile environmental conditions and limited economic and/or technical mitigation alternatives. Cropping systems are regarded as promising tools. The lucerne (Medicago sativa L.)-crop rotation system has long been pushed by policy in the Loess Plateau. However, the adaptability of this system in semi-arid areas is unclear. A 5-year experiment was conducted in Yuzhong, Gansu Province of China (mean annual rainfall 310 mm) to assess adaptability. The results of this study led us to the following conclusions: 1) lucerne is far from a good cropping choice in the semi-arid Loess Plateau in view of ‘immediate’ economic benefit to farmers. Its potential economic benefit needs further investigation considering its value in animal husbandry and ecological service function; 2) lucerne as a high-water-requirement pasture cannot efficiently use water, worse still the soil water deficit caused by lucerne has to be retrieved by the subsequent crop phase; 3) although lucerne has the longest growing season comparing with the annual crops, harvesting in July strongly reduces its soil conservation effectiveness, and the gradual increase in biomass does not offset the effectiveness. Therefore, in the semi-arid Loess plateau with about 300 mm average annual rainfall, inclusion of lucerne in crop rotation cannot serve as an effective cropping system neither for soil conservation purpose, nor for the economic benefits. More cropping options need to be tested to sustain the agroecosystem in this region. | |||
| TO cite this article:Jia Yu,Hu Bin,Zhao Zhi-hong, et al. Assessment of alternative cropping systems in terms of economic benefit, water use and soil conservation: a case study in the semi-arid Loess Plateau, China[OL].[ 6 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4460149 | |||
| 3. Non-additive effects of exotic invasive plant Mikania micrantha H.B.K. litter mixing with native plants | |||
| CHEN Baoming,PENG Shaolin,LI Daijiang | |||
Biology 29 December 2011
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| Abstract:Much research has reported both synergistic and antagonistic effects for decomposition and nutrient release due to the mixing of litter from different species, but few have investigated the effects of non-native litter to date. The exotic plant Mikania micrantha H.B.K. (M. micrantha) has invaded many forests in south China and has altered ecosystem processes. Our previous studies showed that M. micrantha invasion impact the litter decomposition rate and nutrient release greatly through allelopathy, but the effects of mixing M. micrantha litter with native litter are unclear. To quantify litter decomposition rate, a litterbag technique was used. Litter of the invasive plant M. micrantha was mixed with the litter of 7 native plants, respectively, as 3 ratios: M1 (1: 4), M2 (1: 1) and M3 (4: 1). After 60, 128 and 180 days decay, the litter decomposition rate and nutrient (C, N) release were determined. The results showed that there were significant non-additive effects of litter mixing on litter decomposition and litter nutrient dynamics. In addition, we found that non-additive effects (positive or negative) and mixing interaction strength were related to the mixing types (species) ratio and decay process. These results imply that with the degree of M. micrantha invasion, more litter will mix with the litter of native plants, accordingly, which will enhance C and N release from litter. This increase in nutrient release might represent positive feedback to M. micrantha invasion. However, it remains unclear as to why all the different quality litter of 7 native species showed similar mixing effects when mixed with litter of exotic plant M. micrantha, which merits further study. | |||
| TO cite this article:CHEN Baoming,PENG Shaolin,LI Daijiang. Non-additive effects of exotic invasive plant Mikania micrantha H.B.K. litter mixing with native plants[J]. | |||
| 4. Fluctuations in incubation temperature affect incubation duration but not morphology, locomotion and growth of hatchlings in the sand lizard Lacerta agilis (Lacertidae) | |||
| LI Hong,ZHOU Zongshi,DING Guohua,JI Xiang | |||
Biology 31 August 2011
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| Abstract:Studies looking for potential effects of temperature and temperature fluctuations on phenotypic traits of reptile hatchlings have shown species variation, but have not always allowed a distinction between effects of fluctuation per se and temperature extremes themselves. To examine whether incubation temperature fluctuation has a key role in influencing the phenotype of offspring, we incubated eggs of the sand lizard Lacerta agilis at one of the four temperature regimes (27℃, 27±2℃, 27±4℃ and 27±6℃). We found that: (1) hatchlings incubated under the four temperature regimes did not differ from each other in any of morphological and physiological traits examined; (2) interactions that included temperature treatment did not affect any trait examined; (3) the mean incubation length was longer in the 27±6℃ treatment than in the other three treatments; and (4) female hatchlings were shorter in head length and width but longer in SVL as well as abdomen length than males derived from the same sized egg. Our data show that both the type and the magnitude of temperature variation can affect incubation length. We found no evidence for phenotypic divergence in responses to temperature fluctuations during incubation, and therefore suggest that temperature variation does not affect the phenotype of hatchlings in L. agilis. | |||
| TO cite this article:LI Hong,ZHOU Zongshi,DING Guohua, et al. Fluctuations in incubation temperature affect incubation duration but not morphology, locomotion and growth of hatchlings in the sand lizard Lacerta agilis (Lacertidae)[J]. | |||
| 5. Comparative evolution of S7 intron 1 and ribosomal internal transcribed spacer in Coilia nasus (Clupeiformes: Engraulidae) | |||
| LIU Dong,GUO Hongyi,YANG Jinquan,TANG Wenqiao | |||
Biology 19 August 2011
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| Abstract:Intraindividual divergence in ribosomal protein S7 gene intron 1 (rpS7) and the first ribosomal internal transcribed spacer(ITS1) was examined in Coilia nasus to compare the level of variation between single copy rpS7 and multiple copy ITS1 among seven distant populations. Morphology characters, along with mitochondrial COI barcode, employed to identify species validity. A total of 115 clone for rpS7 and 122 clones for ITS1 were obtained from 37 specimens covering this species complex. In clones for rpS7, ten specimens from four population at least presented two different clones by nucleotide substitutions. No variable position was found in five specimens from an isolated population. Variation that fixed in ITS1 was mainly due to different numbers of short base repeat in intra- and inter- specimens, presented in the internal loops and did not disturb functionality in the secondary structures. Numbers of parsimony positions in rpS7 (26) was observably higher than that of ITS1 (2). Comparison between populations found that molecular variance in ITS1 was lower about three times (10.3%) than that of rpS7 (29.9%). Phylogeny tree of C.nasus was not restructured before these rpS7 recombined clones omitted. Together with heterozygosity at nucleotide alternatively fixed in putative parent specimens, a putative hybrid between clades were identified from lineage. This results were explained that the overlap in phenotypic differences of C.nasus complex probably were the consequence of hybrid between discrete populations. Our results indicated that rps7 is more suitable than ITS1 for assessing genetic divergence at intraspecific level. | |||
| TO cite this article:LIU Dong,GUO Hongyi,YANG Jinquan, et al. Comparative evolution of S7 intron 1 and ribosomal internal transcribed spacer in Coilia nasus (Clupeiformes: Engraulidae)[J]. | |||
| 6. Genetic differentiation of Stipa breviflora population in Inner Mongolia and its association with climatic factors | |||
| ZHANG Qing,KANG Sarula,NIU Jianming,DONG Jianjun | |||
| Biology 14 March 2011
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| Abstract:Genetic differentiation of Stipa breviflora population derived from 8 sites in Inner Mongolia and its association with climatic variables was carried out by combining two technologies, which were GIS and means of Random Amplified Polymorphism DNA (RAPD). Twenty-eight primers were screened from 102 arbitrary primers with 10-bp, and a total of 308 bands were produced, including 151 polymorphic bands which account for 49.03%. There were 45 unique loci obtained, accounting for 14.61%. The result showed that 1, the genetic diversity of S. breviflora was rich, but poorer than that of S. grandis and S. krylovii. 2, The diagram of UPGMA cluster agreed with the spatial distribution of this species in the study region greatly.,and Mantel test demonstrated the significantly positive correlation between genetic distance and geographical distance. 3,By CCA and Pearson analysis, significant correlations were found between genetic differentiation and temperature factors(≥10℃ cumulative temperature , ≥0℃cumulative temperature, average temperature in the coldest month, average temperature in a year, average temperature in the hottest month and frost-free period).4,The natural seclection of temperature variation resulted in the genetic differentiation mainly, and genetic flow also played an important roles. | |||
| TO cite this article:ZHANG Qing,KANG Sarula,NIU Jianming, et al. Genetic differentiation of Stipa breviflora population in Inner Mongolia and its association with climatic factors[OL].[14 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4416270 | |||
| 7. Follicle ablation increases offspring size in a lizard with a low clutch frequency | |||
| WANG Zheng,XIA Yuan,JI Xiang | |||
| Biology 04 February 2011 | |||
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| Abstract:Studies of lizards and snakes have shown that an experimental reduction of offspring number sometimes, but not always, results in an increase in offspring size. We applied the "follicle ablation" technique to an oviparous lacertid lizard (Eremias argus) to test the hypothesis that offspring size can be easily altered by manipulating clutch size in species with a low clutch frequency. Our manipulation of clutch size had the effect of inducing variation in egg size in the first post-surgical clutch, with follicle-ablated females producing fewer larger eggs than did controls. Follicle-ablated females produced a second post-surgical clutch as normally as did controls. The proportional amount of resources allocated to reproduction did not shift seasonally in E. argus, but females normally switched from producing a larger number of smaller eggs early in the breeding season to a smaller number of larger eggs later in the season. Females used in this study never produce more than two clutches per breeding season. Therefore, our data validate the hypothesis tested. Our data also provide an inference that maximization of reproductive success could be achieved in females of E. argus by diverting a larger enough, rather than an extraordinarily high, fraction of the available energy to individual offspring in single reproductive episodes.????? | |||
| TO cite this article:WANG Zheng,XIA Yuan,JI Xiang. Follicle ablation increases offspring size in a lizard with a low clutch frequency[OL].[ 4 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4409774 | |||
| 8. Many-lined Sun Skinks (Mabuya multifasciata) Shift Their Thermal Preferences Downwards When Fasted | |||
| LI Hong,MAO Luxi,SHI Linqiang,JI Xiang | |||
| Biology 04 February 2011
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| Abstract:We maintained adult many-lined sun skinks (Mabuya multifasciata; 12 females and 12 males) collected from Hainan (southern China) in September 2006 in two outdoor enclosures to examine the effect of feeding condition on selected body temperature (Tsel). Skinks shifted their thermal preferences when fasted. Temporal variation in Tsel was not found within each sex ? feeding condition combination, and neither in fed nor in fasted skinks did the mean values for Tsel differ between sexes. Body temperatures selected by fed skinks fell within the range from 30.2 to 33.9℃, and those selected by fasted skinks within the range from 25.1to 31.8 ℃. Body temperatures (31.8±0.3 ℃) selected by fed skinks were on average 4.0℃ higher than those (27.8±0.4℃) selected by fasted skinks. Fasted skinks had no difficulty in attaining higher body temperatures, but they voluntarily shift their thermal preferences downwards to save energy. This finding suggests an adaptive mechanism adopted by many-lined sun skinks to enhance their fitness during the periods when food availability is low. | |||
| TO cite this article:LI Hong,MAO Luxi,SHI Linqiang, et al. Many-lined Sun Skinks (Mabuya multifasciata) Shift Their Thermal Preferences Downwards When Fasted[OL].[ 4 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4409780 | |||
| 9. Income breeding and temperature-induced plasticity in reproductive traits in a lacertid lizard, Takydromus septentrionalis | |||
| LUO LaiGao,DING GuoHua,XIANG Ji | |||
| Biology 04 February 2011
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| Abstract:he energy allocation strategy and whether body temperature can affect maternal reproductive traits were examined in a lacertid lizard, Takydromus septentrionalis. We maintained adult females collected from the field soon after winter dormancy under three thermal conditions (24.0 ? 1.0 ?C, 28.0 ? 1.0 ?C, and 32.0 ? 1.0 ?C) throughout the subsequent reproductive season, and then recorded their energy intake, digestive efficiency, body size and mass changes, and reproductive parameters. Manipulation of body temperature in reproducing females affected energy intake and several maternal reproductive characteristics. Females allocated energy to reproduction following the fulfillment of energetic requirements for metabolism and growth. The reduced energy availability for reproduction constrained females at low or high body temperatures to produce fewer and smaller eggs per season, but had no role in influencing the number of eggs produced in single reproductive bouts. Females used currently acquired energy to fuel reproduction, and this strategy did not shift seasonally from the first to subsequent clutches. Our study supports the idea that the use of currently acquired energy for reproduction may be more widespread in ectotherms than thought before, and shows for the first time that egg size is thermally plastic in T. septentrionalis, where eggs produced in single reproductive bouts are believed to be well optimized for size. | |||
| TO cite this article:LUO LaiGao,DING GuoHua,XIANG Ji. Income breeding and temperature-induced plasticity in reproductive traits in a lacertid lizard, Takydromus septentrionalis[OL].[ 4 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4409786 | |||
| 10. esponse of photosynthetic and morphological characteristics to soil moisture stress for Mosla chinensis and congeneric weediness M. scabra | |||
| Yijun Lu ,Ge Ying,Gu Baojing,Guan Baohua,Chang Jie | |||
| Biology 11 March 2010 | |||
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| Abstract:In the field investigation, we found populations of a rare species Mosla chinensis and congeneric weediness species M. scabra distribute in drought habitats frequently, while they have different performances. In order to find out how the two species response to water stress in physiology and morphology, we compared the characteristics of the two species under different soil water status in cultivation. Results showed that the largest folia diurnal net photosynthetic rate (PN), stomatal conductance (gs), water use efficiency (WUE), apical height (H) and crown diameter (CD) of M. chinensis and M. scabra were occurred at 60% and 90% soil water holding capacity (WHC) respectively, while the smallest values occurred at 20% WHC in both two species. The PN, gs, WUE, H and CD of M. chinensis were larger than those of M. scabra, while the chlorophyll a (Chl a) and Chl b contents, the physiological plasticity indices were smaller than those of M. scabra, which is more sensitivity and has smaller drought resistance to soil water status transform than M. chinensis. The height concerned traits of M. scabra increased with the increase of soil moisture, it showed that M. scabra was a resource, which increased the height to obtain more irradiance at moist soil. However, the highest values for the height concerned traits of M. chinensis happened in W60 and less than M. scabra indicated M. chinensis could not enhanced the competitive ability for irradiation and failure in community under well water conditions and become endurant species. | |||
| TO cite this article:Yijun Lu ,Ge Ying,Gu Baojing, et al. esponse of photosynthetic and morphological characteristics to soil moisture stress for Mosla chinensis and congeneric weediness M. scabra[OL].[11 March 2010] http://en.paper.edu.cn/en_releasepaper/content/40575 | |||
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