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	1. Analyzation of relationship between soil nutrients and podophyllotoxin accumulation in Podophyllum hexandrum and Dysosma versipellis
	LI Mengfei,ZHANG Jing,LI Jinghua,ZHOU Lanlan,LI Tiantian,LI Wei
	Biology 06 March 2013
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	Abstract:In order to analyze the correlation between soil nutrients and PDT accumulation. The experiment was designed to determine the PDT content in rhizomes of P. hexandrum and D. versipellis by high performance liquid chromatography (HPLC) and nutrients content in soil by high performance ion chromatography (HPIC) and high-resolution continuum source flame atomic absorption spectrometry (HR-CS FAAS). The results of linear regress analysis showed that the PDT accumulation was positive relationship with NO3-, PO43-, Na+, Fe, Mn, and negative relationship with SO42-, K+, while Mg2+, Ca2+, Cu and Zn didn't reach significant levels. This study information can be helpful to produce PDT and related cyclolignans by commercial cultivation, cell and tissue cultures of P. hexandrum, D. versipellis and other related plant sources.
	TO cite this article:LI Mengfei,ZHANG Jing,LI Jinghua, et al. Analyzation of relationship between soil nutrients and podophyllotoxin accumulation in Podophyllum hexandrum and Dysosma versipellis[OL].[ 6 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4526883


	2. Expression of anthocyanin biosynthetic genes during fruit development in Fengxiang strawberry
	ZHOU Bo,LI Yuhua
	Biology 01 February 2012
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	Abstract:Anthocyanins are the main pigments in flowers and fruits. In most cases, anthocyanin accumulation in fruit is highly controlled by the developmental level. In this study, the cDNA fragments of three genes, chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS), which are involved in the flavonoid pathway, were isolated from total RNA of strawberry ripe fruit by using polymerase chain reaction technique and labeled as probes to determine the expression of anthocyanin biosynthetic genes. Northern analysis showed that a correlation between anthocyanin accumulation and expression of the flavonoid pathway genes during the ripening of strawberry fruits. At the early stages of fruit development, the mRNA levels encoding CHS, DFR, ANS were high probably responsible for the accumulation of condensed tannins, but the levels decreased dramatically when fruits turned white from green. During the stage of pigment accumulation, their mRNA levels increased strongly to be involved anthocyanin biosynthesis. Difference of CHS in mRNA abundance was correlated with differential accumulation of anthocyanins throughout the process of fruit development. Therefore, CHS could be a key structure gene involved in anthocyanin synthesis. Furthermore, the co-ordination of expression of anthocyanin biosynthetic genes implied a common regulatory mechanism controlling the expression of structural genes in the flavonoid pathway.
	TO cite this article:ZHOU Bo,LI Yuhua. Expression of anthocyanin biosynthetic genes during fruit development in Fengxiang strawberry[OL].[ 1 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4464645


	3. Sexual differences in photosynthetic characteristics of Ginkgo during leaf senescence
	Dawei Shi,Xiaodong Wei,Guoxiang Chen,Yanli Xu
	Biology 13 January 2010
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	Abstract:Photosynthetic characteristics were compared between female and male ginkgoes, to investigate the differences in photosynthetic decline of leaves between two species during leaf senescence. Photosynthetic rate was used as a biomarker to define the initiation of leaf senescence which could be divided two stages. Therefore, the leaf senescence of ginkgo occurred at late August which could be the beginning of autumn. During the early stages of leaf senescence, photosynthetic functions, such as the electron transport activities of photosystems and photophosphorylation, and scavenging activities were lower in female than in male. But at the late senescence, these characteristics of female were higher those of male. With leaf aging, the damage to chloroplast of female induced by senescence was more than that of male. Our results found that there was a similar rate of leaf senescence between female and male, which may be the reason why the ginkgo has declined during the evolution of plant senescence.
	TO cite this article:Dawei Shi,Xiaodong Wei,Guoxiang Chen, et al. Sexual differences in photosynthetic characteristics of Ginkgo during leaf senescence[OL].[13 January 2010] http://en.paper.edu.cn/en_releasepaper/content/38873


	4. Effects of low temperature on membrane lipid peroxidation in a newly developed super-high-yield hybrid rice (Oryza sativa L.) at seedling stage
	Dawei Shi,Xiaodong Wei,Guoxiang Chen,Chengjun Zhang,Ping Wang,Jing Wang,Chuangen Lv
	Biology 23 November 2009
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	Abstract:To study the effects of low temperature on high-yield hybrid rice (Oryza sativa L.) at seedling stage, chlorophyll content, membrane lipid peroxidation, fatty acids composition and chloroplast ultrastructure of two varieties were investigated—a newly-developed cultivated super-high-yield hybrid rice LYPJ, and a traditional hybrid rice SY-63, setting 25℃ as control and 10℃ as low temperature treatment. In SY-63, chlorophyll content in rice leaves was decreased obviously with the duration at low temperature, whereas it did not decrease statistically in LYPJ. MDA (malondialdehyde) content increased slightly in LYPJ, but in SY-63, MDA content increased higher than those in 25℃-treated seedlings. The contents of saturated fatty acids decreased and the contents of unsaturated fatty acids increased in both of the two hybrids compared to the control. The IUFA (index of unsaturated fatty acid) of fatty acids increased in the two cultivars, being lower in LYPJ. The damage to the ultrastructure of chloroplasts was not so serious with increasing cold stress, but the number of osmiophilic granules in SY-63 increased significantly. The results indicated that LYPJ could have stronger chilling-resistance than SY-63 at seedling stage.
	TO cite this article:Dawei Shi,Xiaodong Wei,Guoxiang Chen, et al. Effects of low temperature on membrane lipid peroxidation in a newly developed super-high-yield hybrid rice (Oryza sativa L.) at seedling stage[OL].[23 November 2009] http://en.paper.edu.cn/en_releasepaper/content/36947


	5. Comparative studies of senescence-related enzymes in the cotyledon of chlorophyll b–deficient mutant and its wild type oilseed rape during senescence
	Tingting Qin,Jingfeng Fu,Nianhui Zhang,Linfang Du
	Biology 28 November 2005
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	Abstract:The change patterns of senescence-related enzymes during cotyledon senescence were studied in a chlorophyll (Chl) b-deficient mutant type (MT) and its wild type (WT) of Brassica napus L. seedlings. The fresh weight on the basis of cotyledon number initially increased till 20 days after planting (DAP) and then kept relative constant. The protein content decreased sharply since 20 DAP and Chl content reduced since 10 DAP in both two types; however the rate of degradation in protein and Chl in the MT was slower than that in the WT since 20 DAP. Superoxide dismutase (SOD; E.C.1.15.1.1) activity declined in the WT but increased in the MT since 20 DAP. Activity of peroxidase (POD; E.C.1.11.1.7) increased markedly after 20 DAP in both types. Esterase (EST; E.C.3.1.1.1) activity increased in both two types since 10 DAP, whereas at 40 DAP it was much lower in the MT than that in the WT. In addition, bands patterns of SOD, POD and EST isozymes were changed during cotyledon development in both two types, but some differences were observed. Cu/ZnSODs activities were higher in the MT at 40 DAP as compared with the WT. These results showed that day 20 was the turning point during the cotyledon development and the senescence in Cr3529 cotyledon was slower than that in the WT.
	TO cite this article:Tingting Qin,Jingfeng Fu,Nianhui Zhang, et al. Comparative studies of senescence-related enzymes in the cotyledon of chlorophyll b–deficient mutant and its wild type oilseed rape during senescence[OL].[28 November 2005] http://en.paper.edu.cn/en_releasepaper/content/3804

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