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	1. cDNA Cloning ,Pituitary Location and Extra-pituitary Expression of Pro-opiodmelanocortin (POMC) Gene in Rare Minnow (Gobiocypris rarus)
	Liu Xiaohong,Xie Biwen,Zhang Yaoguang,Wang Deshou,Wang Zhijian
	Biology 27 September 2009
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	Abstract:A cDNA encoding pro-opiodmelanocortin (POMC) was cloned from pituitary of the rare minnow ( Gobiocypris rarus), a small freshwater fish endemic to China ，by polymerase chain reaction(RT-PCR) and rapid amplification of cDNA ends (RACE). Data showed that, the predicted rare minnow POMC cDNA (rmPOMC) consisted of 844bp with a 666bp ORF coding for the following sequences flanked by proteolytic cleavage sites: signal peptide (SP, Met1–Ala28), N-terminal peptide (Gln29–His105), ACTH (Ser108–Met146), α-MSH ( Ser108–Gal121), CLIP (Pro126–Met146), β-LPH (Glu149–His221), γ-LPH (Glu149– Ser186), β-MSH (Asp 170–Ser186), β-endorphin (β-EP, Tyr189–Gln221). Sequence analysis showed no region homologous to γ-MSH/ joining peptide (a tetrapod pomc feature ) was found. Amino acid sequence shares a highest similarity with POMC-Ⅰand POMC-Ⅱof common carp (92.4%) according to homologous alignment. Pituitary and extra-pituitary expression were studied by RT-PCR and in situ hybridization. rmPOMC positive cells mainly located in rostral pars distails (RPD) and pars intermedia(PI). Otherwise, there were some rmPOMC positive cells detected in PPD too according to in situ hybridization. In the extra-pituitary tissues, positive signals were observed in brain, intestine, gonad, hepatopancreas, spleen and gill by RT-PCR analysis.
	TO cite this article:Liu Xiaohong,Xie Biwen,Zhang Yaoguang, et al. cDNA Cloning ,Pituitary Location and Extra-pituitary Expression of Pro-opiodmelanocortin (POMC) Gene in Rare Minnow (Gobiocypris rarus)[OL].[27 September 2009] http://en.paper.edu.cn/en_releasepaper/content/35503


	2. Cross-species microsatellites amplification for a vulnerable species endemic of China, Reeves’s pheasant (Syrmaticus reevesii)
	Wang Ning,Chang Jiang,Sun Quanhui,Gu Langyu,Zhangzhengwang
	Biology 25 February 2009
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	Abstract:Cross-species microsatellite amplification can be an effective way in obtaining microsatellite loci for closely related taxa in bird species. The Reeves’s pheasant, Syrmaticus reevesii, is a vulnerable species endemic of China. In order to study the population genetics and paternity of its wild populations, we obtained 10 polymorphic microsatellite markers from cross-species amplification. The number of alleles per locus varies between two and twelve, with expected heterozygosity ranging from 0.32 to 0.802 (n =75). These polymorphic loci are facilitating the study of paternity identification of Reeves’s pheasant and also provide a valuable tool for future studies on conservation genetics of this species.
	TO cite this article:Wang Ning,Chang Jiang,Sun Quanhui, et al. Cross-species microsatellites amplification for a vulnerable species endemic of China, Reeves’s pheasant (Syrmaticus reevesii) [OL].[25 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29630


	3. The apical-basalateral polarization of RPE
	Jia Yanjuan,Song Dexiao,Liu Yong,Gao Lan
	Biology 18 February 2009
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	Abstract:Vertebrate retinal pigment epithelium (RPE) is a highly specialized epithelium with polarized membrane domains，with microvilli and junctional complexes on the apical pole and infoldings along the basolateral side, which express a morphological and functional polarity along their apical-to-basal axis. The loss of RPE polarity is associated with many degenerative eye diseases. Here, we highlight the polarized distribution of membrane protein and melanosome, TJs development, IPM and αPKC’s role and the alteration of RPE polarity in regeneration. Although RPE polarity has been studied a lot, which is at infant stage for treating degenerative eye diseases. So many morphological events and regulated mechanism still need to be elucidated in greater details from histological to molecular level, from development to adult, from nomal to mutation.
	TO cite this article:Jia Yanjuan,Song Dexiao,Liu Yong, et al. The apical-basalateral polarization of RPE[OL].[18 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29249


	4. The computed tomography and gross anatomies of nasal cavity and sinuses in the Bactrian camel (Camelus bactrianus)
	Zhong-Tian Bai,Hong-Ju Wang,Jing-Chen Chen,Guo-Qiang Yuan,Lian-Lian Li,Jian-Lin Wang
	Biology 05 December 2008
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	Abstract:The structures of nasal cavity and sinuses of the Bactrian camel and their adaptability to living environments were investigated using 64-slices spiral computed tomography (CT) and gross anatomy. The results show that the Bactrian camel owns large and complex concha, narrow nasal meatus and large sinuses in volume. All these properties may relate to its living conditions of frequent sand-storms and aridity. This will offer the important references to the research of nasal morphology and adaptive physiological mechanism to its environment. And the present results and methods present useful references to the construction of nasal airflow model in the Bactrian camel.
	TO cite this article:Zhong-Tian Bai,Hong-Ju Wang,Jing-Chen Chen, et al. The computed tomography and gross anatomies of nasal cavity and sinuses in the Bactrian camel (Camelus bactrianus)[OL].[ 5 December 2008] http://en.paper.edu.cn/en_releasepaper/content/26364


	5. Cleantioides longicornis, a new species of Holognathidae (Crustacea, Isopoda, Valvifera) in the Yangtze Estuary, China
	Liu Wenliang ,Poore Gary C. B.,Lu Jianjian
	Biology 18 September 2008
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	Abstract:Cleantioides longicornis, a new species, is described on the basis of specimens collected from Yangtze estuary, Shanghai, China. The new species can be distinguished from the closely related species, C. albaniensis Poore & Lew Ton, by the narrower antenna 2; a slightly notched pleotelson; arrangements of spines or pectinate spines on preopods.
	TO cite this article:Liu Wenliang ,Poore Gary C. B.,Lu Jianjian . Cleantioides longicornis, a new species of Holognathidae (Crustacea, Isopoda, Valvifera) in the Yangtze Estuary, China[OL].[18 September 2008] http://en.paper.edu.cn/en_releasepaper/content/24166


	6. Rapid and Sensitive Detection of Singapore grouper iridovirus by Loop-Mediated Isothermal Amplification
	Mao Xinliang,Zhou Sheng,Xu Dan,Gong Jie,Cui Huachun,Qin Qiwei ,Qin Qiwei
	Biology 24 December 2007
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	Abstract:Aims: The aim of this paper is to develop a loop-mediated isothermal amplification (LAMP) method for rapid, sensitive and inexpensive detection of SGIV in grouper, Epinephelus sp. Methods and Results: A set of six specific primers was designed by targeting the SGIV ORF-014L. With Bst DNA polymerase large fragment, the target DNA can be amplified as early as 20 min at 65°C in a simple water bath. The detection limit is about 0.02 fg (equivalent to 6.3 copies) of plasmid ORF-014L. LAMP products could be judged with three different methods. There were no cross reactions with 7 other aquatic animal viruses indicating high specificity of the LAMP. The LAMP method was applied to detect SGIV in virus infected GP cells and grouper tissues effectively. Conclusions: The LAMP described in this study is a cheap, sensitive, specific and rapid protocol for detection of SGIV in cells and in grouper tissues. Significance and Impact of the Study: The developed LAMP method can be simply applied both in field condition and in laboratory operation for specific detection of SGIV infection.
	TO cite this article:Mao Xinliang,Zhou Sheng,Xu Dan, et al. Rapid and Sensitive Detection of Singapore grouper iridovirus by Loop-Mediated Isothermal Amplification[OL].[24 December 2007] http://en.paper.edu.cn/en_releasepaper/content/17288


	7. Observation and Quantitative Compare of P-like cells in Right Atrium between Male and Female Mice
	Yuan Guoqiang,Bai Zhongtian,Wang Jianlin
	Biology 06 September 2007
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	Abstract:Aim To investigate the morphological basis on which sex differences in the probability of happening atrial fibrillations by observing morphologically and comparing quantitatively P-like cells in the right atrium between male and female mice. Methods 20 adult healthy Wistar mice ware used in the experiment， male and female mice ten respectively. Wright atrias of this mouse were dissected. Serial sections from this tissue were stained with hematoxylin and eosin. Then slides were examined， measured and photographed by light microscope. Results It was indicated that cell acreage， nuclear acreage and the ratio between them of P-like cells in the right atrium from male and female were different. Conclusion The morphological differences of P-like cells in the right atrium from male and female may be the reason that leaded to sex differences in happening atrial fibrillations.
	TO cite this article:Yuan Guoqiang,Bai Zhongtian,Wang Jianlin. Observation and Quantitative Compare of P-like cells in Right Atrium between Male and Female Mice[OL].[ 6 September 2007] http://en.paper.edu.cn/en_releasepaper/content/14918

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