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1. Production of 1,4-butanediol from succinic acid using Escherichia coli whole-cell catalysis | |||
Ping Ni,Cong Gao,Jing Wu,Wei Song,Xiaomin Li,Wanqing Wei,Xiulai Chen,Liming Liu | |||
Biology 27 February 2024 | |||
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Abstract:The microbial production of 1,4-butanediol (1,4-BDO), a crucial bulk chemical used in the organic chemical and food industries, has attracted increasing attention due to environmental concerns. However, its biosynthetic efficiency via current biotransformation routes is limited. In this study, a dual-pathway approach for 1,4-BDO production from succinic acid was developed. Specifically, a double-enzyme catalytic pathway involving carboxylic acid reductase and ethanol dehydrogenase was proposed. Optimization of the expression levels of the pathway enzymes led to a significant 318% increase in 1,4-BDO titer. Additionally, the rate-limiting enzyme MmCar was engineered to enhance substrate affinity by 50% and increase 1,4-BDO titer by 46.7%. To address cofactor supply limitations, an NADPH and ATP cycling system was established, resulting in a 48.9% increase in 1,4-BDO production. Ultimately, after 48 hours, 1,4-BDO titers reached 201 mg/L and 1555 mg/L in shake flask and 5 L fermenter, respectively. This work represents a significant advancement in 1,4-BDO synthesis from succinic acid, with potential applications in the organic chemical and food industries. | |||
TO cite this article:Ping Ni,Cong Gao,Jing Wu, et al. Production of 1,4-butanediol from succinic acid using Escherichia coli whole-cell catalysis[OL].[27 February 2024] http://en.paper.edu.cn/en_releasepaper/content/4762275 |
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