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1. 河南地区猪札幌病毒的遗传多样性研究 | |||
Quan Shen,Xiaochun Wang,Rong Fu,Xin Geng,Chengcheng Tao,Zeyu Li,Li Wang | |||
Biology 30 November 2015 | |||
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Abstract:Sapoviruses (SaVs) belong to the family Caliciviridae and are related to gastroenteritis of humans and animals. These agents have been reported from several countries of the world. In this study, A total of 169 stool samples from pigs were collected from 4 pig farms located in Henan Province in central China from January to February 2015. Specimens were tested by reverse transcription polymerase chain reaction (RT-PCR) using the primers p289/p290 designed to detect the polymerase gene of caliciviruses, including both SaVs and noroviruses (NoV). Results showed that three of the four farms wre positive for SaVs and the positive rates were 12.8% 15.6%, and7.1%, respectively. Overall, fiften of 169 (8.9%) stool samples were positive for SaVs. Ten of distinct SaVs sequences were confrimed by sequencing. Phylogenetic analysis based on the partial sequences of the RNA-dependent RNA polymerase (RdRp) gene indicated that these SaVs were divided into five distinct genogroup, GIII, GVII, GVIII, and two potential new genogroup GIX and GX. Four strains belonged to GIII, three stains belonged to GVII, and one belonged to GVIII, GIX or GX, respectivily. No NoV infection were detected in the current study. To the best of our knowledge, this is the first report that GVIII, GIX and GX SaVs infection detected in Chian. Furthermore, the high genetic variability and prevalence of SaVs infection provides evidence that different genogroups of SaVs are circulating in Chinese pig herds. | |||
TO cite this article:Quan Shen,Xiaochun Wang,Rong Fu, et al. 河南地区猪札幌病毒的遗传多样性研究[OL].[30 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4664930 |
2. Genomic dsRNA of Grass carp reovirus protected from both the trigger and effector nucleases of RNA interference pathway during viral replication | |||
Guo Shuai,Lu Liqun | |||
Biology 28 March 2011 | |||
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Abstract:Objective: The present study aimed to investigate the effect of GCRV replication on Dicer-initiated RNAi pathway of host cell. Methods: The pEGFP-N1 was transfected into Grass carp kidney cells (CIK) with either the synthetic EGFP-speific siRNA or in intro transcribed EGFP-specific dsRNA to signify the functional gene silencing pathway in CIK cell line. Then, CIK cells were infected with GCRV and the steady-state transcription levels of Dicer were monitored during viral replication cycle using real-time quantitative PCR (qRT-PCR). The effect of GCRV replication on RNA interference pathway was further studied by investigating the silencing of egfp gene by egfp-siRNA in GCRV-infected CIK cells. Results: both egfp-siRNA and egfp-dsRNA could silence the egfp reporter gene in uninfected CIK cells. In GCRV-infected cells, replication of GCRV correlated with the increased transcription level of Dicer gene while resulted in the loss function of egfp-siRNA on silencing reporter egfp gene. Conclusion: Replication of GCRV augmented the transcription of Dicer gene but still antagonized the classical RNAi pathway of host cells. Suppression of the siRNA-induced RNAi by GCRV replication suggested that GCRV could inhibit the effector protein of RNAi pathway besides the insensitivity of its dsRNA genome to Dicer nuclease, the RNAi- trigger nuclease. | |||
TO cite this article:Guo Shuai,Lu Liqun. Genomic dsRNA of Grass carp reovirus protected from both the trigger and effector nucleases of RNA interference pathway during viral replication[OL].[28 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4418341 |
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