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1. Vaccine potential analysis of Grass carp reovirus capsid protein VP7 through in vitro microneutralization assay | |||
Yang Qian,Lu Liqun | |||
Biology 28 March 2011 | |||
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Abstract:In this paper, the Glutathione S-transferase (GST) fusion protein expression vector pGEX-4T-3 was employed to clone and expression of GCRV outer capsid gene vp7, which was amplified by RT - PCR from infected Grass carp. The recombinant GST-fusion protein rVP7 was induced by 1mM IPTG in Dh5αand confirmed by SDS-PAGE and Western blot assays using both anti-GST-tag and anti-VP7 monoclonal antisera. An expected 52-kDa rVP7 was highly expressed, and was mainly exhibited in the formation of the inclusion body. After purification, rVP7 was intraperitoneally injected to the experimental mice to produce anti-rVP7 polyclonal serum. In vitro microneutralization assay indicated that polyclonal antibody against rVP7 could neutralize GCRV, and suggested that rVP7 had the potential to be used as subunit vaccine against GCRV infection. The present study paved the way for further characterization of the immunogenicity of viral outer capsid protein VP7 in grass carp Ctenopharyngodon idellus and could be based to develop antibody or antigen detection assays for GCRV pathogen. | |||
TO cite this article:Yang Qian,Lu Liqun. Vaccine potential analysis of Grass carp reovirus capsid protein VP7 through in vitro microneutralization assay[OL].[28 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4418350 |
2. Transgenic rice plants expressing rice dwarf virus outer coat protein gene (S8) are moderately resistant to virus infection | |||
Zheng Honghong,Wei Chunhong,Chen Shengxiang,Chen Zhangliang,Y. Li | |||
Biology 19 December 2005 | |||
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Abstract:We have previously obtained transgenic rice plants (Oryza sativa L.) containing S8 gene which encodes the outer coat protein of the rice dwarf virus (RDV) by particle bombardment. In this study, it was confirmed that the S8 gene has been stably inherited and expressed in the selfed T1 and T2 progenies of the primary trangenic rice plant line T0-2. Virus resistance assays revealed that both T1 and T2 transgenic plants exhibited resistance to RDV infection, indicating stable transmission of the trait of resistance. This study is the first report about the Pathogen-derived resistance in rice dwarf virus, a member of phytoreovirus. | |||
TO cite this article:Zheng Honghong,Wei Chunhong,Chen Shengxiang, et al. Transgenic rice plants expressing rice dwarf virus outer coat protein gene (S8) are moderately resistant to virus infection[OL].[19 December 2005] http://en.paper.edu.cn/en_releasepaper/content/4449 |
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