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	1. Characterization of Acetobacter pasteurianus CGMCC 3089 during evolutionary adaptation to ethanol
	Zheng Yu,Han Qi,Zhang Keping,Wang Min
	Biology 25 October 2012
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	Abstract:Acetobacter pasteurianus is usually used for vinegar fermentations, however, the growth of A. pasteurianus was commonly inhibited by high ethanol concentration. In this research, the ethanol resistance of A. pasteurianus CGMCC 3089 was improved by a continuous ethanol stress adaptation culture. The characterization of A. pasteurianus CGMCC 3089 during the evolutionary process of adaptation to ethanol was performed with respect to the improved resistance to ethanol. It was found that the ethanol resistance of A. pasteurianus was related to the activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenases (ALDH). Furthermore, the improved resistance against ethanol was an inheritable phenotype, instead of a transient physiologic adaptation. The results of Random Amplified Polymorphic DNA (RAPD) suggested that there was some diversity between the genomes of the original strain and the evolutionary strain.
	TO cite this article:Zheng Yu,Han Qi,Zhang Keping, et al. Characterization of Acetobacter pasteurianus CGMCC 3089 during evolutionary adaptation to ethanol[OL].[25 October 2012] http://en.paper.edu.cn/en_releasepaper/content/4493041


	2. High cell-density production of glucagon-like peptide-1 derivate (GP62) in recombinant E.coli by optimization of culture medium
	Jiang Deqi,Ran Yanhong,Chen Ying,Sang Yanxia,Zhou Tianhong,Li Hongjian
	Biology 11 February 2011
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	Abstract:This article aims to get high level expression of GLP-1 derivate (GP62) in high cell-density recombinant Escherichia coli BL21(DE3) by optimizing the fed-batch culture medium. The effects of different basal mediums, yeast extract and inorganic salts concentration on cell growth and expression of GLP-1 derivate (GP62) in recombinant E.coli BL21(DE3) were investigated by single factor test and orthogonal array. Based on the 2YT mediun, get the optimun 2YT-GP62 medium. The highest productivity of 30.0 mg l-1 GLP-1 derivate (GP62) and the ?nal cell density 21.3 (OD600) had been obtained in 5L fermentor induced with 0.6 mmol l-1 IPTG. The final cell density was 4.4 times higher than that in LB culture medium and the expression level of GP62 improved from 27.5% to 31.2%. The results showed that 2YT-GP62 medium can be used for recombinant GLP-1 derivate batch cultivation.
	TO cite this article:Jiang Deqi,Ran Yanhong,Chen Ying, et al. High cell-density production of glucagon-like peptide-1 derivate (GP62) in recombinant E.coli by optimization of culture medium[OL].[11 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4410546

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	3. Optimal conditions for producing microalgal oil with high oleic acid content from Chlorella vulgaris LB 112
	Zhang Wei,Wu Hong,Zong Minhua
	Biology 07 July 2009
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	Abstract:Five different microalgae, including Chlorella vulgaris 24, Chlorella vulgaris LB112, Chlorella pyrenoidosa 27, Chlorella pyrenoidosa LB137, Chlorella pyrenoidosa LB308, were investigated for the purpose of screening out a strain producing lipid with high oleic acid content and Chlorella vulgaris LB112 was found to be able to accumulate lipid of high oleic acid content and unsaturated fatty acid content. Effects of culture conditions and culture medium components on the cell growth, lipid accumulation as well as lipid composition of Chlorella vulgaris LB112 were further investigated. The optimal carbon source, nitrogen source and C/N molar ratio were glucose, glycine, and 357, respectively. The supplement of small amount of divalent metal ions including Mg2+ and Fe2+ was beneficial to cell growth and lipid accumulation of Chlorella vulgaris LB112. The favorable temperature, initial pH of medium, light intensity and shaking rate were 25 °C, 6.0, 1000 lux and 130 r min-1, respectively. Under the optimum conditions, a biomass of 4.67 g l-1 and a lipid content of 47.58%, which are much higher than the original values (2.20 g l-1 and 34.55%) before optimization, could be obtained after culture for 7 days. The microalgal oil mainly contains 7.44% palmitic acid, 2.78% palmitoleic acid, 6.58% stearic acid, 68.10% oleic acid, 10.07% octadecadienoic acid, 4.02% octadecatrienoic acid and the total unsaturated fatty acids content reaches around 85%.
	TO cite this article:Zhang Wei,Wu Hong,Zong Minhua. Optimal conditions for producing microalgal oil with high oleic acid content from Chlorella vulgaris LB 112[OL].[ 7 July 2009] http://en.paper.edu.cn/en_releasepaper/content/33683


	4. Expression and purification of NSH2 domain protein for NMR research
	Niu Gao,Guo Jiangfeng,Zhang Yaozhou
	Biology 06 March 2009
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	Abstract:SHP-2 protein, which has two SH2 domains, is essential for the embryonic development, haematopoiesis and signaling downstream of a variety of growth factors. SHP-2 proteins are related to many diseases. To facilitate fundamental studies, it is important that the proteins can be expressed in high quality and in a large quantity. In this work, the amino-terminal SH2 (NSH2) domain of SHP-2 protein which is important for the protein’s self-regulation was recombinated into the prokaryotic expression vector, pGEX-2T, and introduced into E. coli BL21 for a prokaryotic expression. Two recombinant vectors with different extra amino acid residues were designed on the basis of properties of NSH2 protein and the sequence of expression vector, pGEX-2T, to produce NSH2 protein with a high property. The effects of the two groups of different extra amino acid residues on solubility and stability were compared. The comparisons indicated that end extra amino acid residues may have strong effects on both solubility and stability. The higher soluble and stable NSH2 protein was selected and labeled with isotope 15N for NMR study. The high resolution of NMR demonstrated the correction folding of the protein and the interactions of the protein with phosphop-tyrosin peptides.
	TO cite this article:Niu Gao,Guo Jiangfeng,Zhang Yaozhou. Expression and purification of NSH2 domain protein for NMR research[OL].[ 6 March 2009] http://en.paper.edu.cn/en_releasepaper/content/30007


	5. The study on hemo-compatibility of hydrophilic and hydrophobic SiCOH plasma coating
	WANG Gui-xue,ZHANG Qin,SHEN Yang,GE Shu-ping,JIA Dong-yu,TANG Chao-jun,YU Qing-song
	Biology 23 February 2009
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	Abstract:Low- temperature plasma deposition technique was used to prepare hydrophilic and hydrophobic SiCOH coating on the surface of silicon wafer. The flow ratio of two kinds of monomers, TMS and O2 could be controlled to obtain different hydrophilic plasma SiCOH coating. The results showed that, with increase of flow rate of O2, the hydrophilicity of surface with SiCOH coating could be improved, the water contact angle decreased from 105.8±3.0°to 29.8±2.1°. The results of blood proteins and platelets adhesion, activated partial thromboplastin time (aPTT) also showed that the blood-compatibility could be enhanced with the increase of hydrophilicity of plasma SiCOH coating.
	TO cite this article:WANG Gui-xue,ZHANG Qin,SHEN Yang, et al. The study on hemo-compatibility of hydrophilic and hydrophobic SiCOH plasma coating[OL].[23 February 2009] http://en.paper.edu.cn/en_releasepaper/content/29506


	6. Economical propionic acid production from cane molasses by Propionibacterium freudenreichii CCTCC M207015 in a plant fibrous-bed bioreactor
	Xiaohai Feng,Bo Wu,Hong Xu,Hanjie Ying,Pingkai Ouyang
	Biology 16 January 2009
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	Abstract:Propionic acid was produced in a plant fibrous-bed (PFB) bioreactor by Propionibacterium freudenreichii CCTCC M207015. Sugar cane bagasse and cane molasses, as the by-products of sugar-refinery, were used as cell immobilized material and carbon source respectively for propionic acid production. For the non-treated cane molasses, 62.08 g l–1 of propionic acid was produced after 254 h of fermentation with a productivity of 0.24 g h–1 l–1. Hydrolyzed cane molasses was also applied for the propionic acid production. The maxiumun propionic acid yield reached 91.87 g l–1 after 254 h fermentation with a productivity of 0.36 g h–1 l–1. The PFB bioreactor exhibited excellent production stability during batch fermentation and the propionic acid yield remained high after ten batch of propionic acid production.
	TO cite this article:Xiaohai Feng,Bo Wu,Hong Xu, et al. Economical propionic acid production from cane molasses by Propionibacterium freudenreichii CCTCC M207015 in a plant fibrous-bed bioreactor[OL].[16 January 2009] http://en.paper.edu.cn/en_releasepaper/content/27931


	7. Characterization of HMW Glutenin Subunits in Bread and Tetraploid Wheats by Reversed-Phase High-Performance Liquid Chromatography
	Dong Kun,Hao Chun-yan,Wang Ai-li,Cai Min-hua,Yan Yue-ming
	Biology 15 January 2009
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	Abstract:The wheat storage proteins, especially the high molecular weight glutenin subunits (HMW-GS), play important roles in the determination of flour processing and bread-making quality. Compared with the traditional SDS-PAGE method, reversed-phase high-performance liquid chromatography (RP-HPLC) was shown to have many advantages for the separation and characterization of HMW-GS because of its high resolving power, repeatability and automation. In this work, HMW-GS from bread and tetraploid wheats were separated and characterized by RP-HPLC. The elution time ranking of different HMW-GS was: 1Ax>1Bx>1Dx>1By>1Dy. Several subunit pairs associated with good quality properties and those with similar mobilities on SDS-PAGE, such as 1Bx7 and 1Bx7, 1By8 and 1By8, 1Dx2 and 1Ax2*, 1Bx6 and 1Bx6.1, were well separated and readily identified through RP-HPLC. However, other subunit pairs, such as 1Dy10-1Dy12, 1Dx5-1By18 and 1Dx2-1By16, could not be adequately separated and identified by RP-HPLC, whereas they displayed different mobilities on SDS-PAGE gels. Because 1Dx5 and 1Dx2 showed different hydrophobicities, RP-HPLC could distinguish 1Dx5+1Dy10 and 1Dx2+1Dy12. A comparative analysis between RP-HPLC and SDS-PAGE showed that a combination of both methods provided more effective identification of HMW-GS in wheat quality improvement and germplasm screening.
	TO cite this article:Dong Kun,Hao Chun-yan,Wang Ai-li, et al. Characterization of HMW Glutenin Subunits in Bread and Tetraploid Wheats by Reversed-Phase High-Performance Liquid Chromatography[OL].[15 January 2009] http://en.paper.edu.cn/en_releasepaper/content/27867


	8. Improve on recovery of the recombinant human stem cell factor inclusion body in refolding with simultaneous purification process for large-scale
	Lili Wang,Chaozhan Wang ,Jiangfeng Liu,Xindu Geng
	Biology 06 March 2008
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	Abstract: Recombinant expressed human stem cell factor (rhSCF) as cytoplasmic inclusion bodies (IB) are reported. In present work, to increase mass recovery of rhSCF production in large scale, the factors affecting about efficiency of rhSCF IB were recovered and solubilised in urea solution, and refolding with simultaneous purification process using protein folding liquid chromatography (PFLC) were investigated, including normal chromatographic column, the unit for the simultaneous renaturation and purification of proteins (USRPP). Finally, by combining the optimized buffer and USRPP, we were able to obtain 22 mg rhSCF with >95% purity.The mass recovery is 24 % for dilution, 38 % for the normal chromatographic column, 49 % for the USRPP. An average specific bioactivity is 4.27 ×105 IU/mg, 6.9×105 IU/mg, and 1.28×106 IU/mg, respectively. These protocol dates and new refolding with purification method -USRPP provide a cost effective and an efficient way to produce quantities of high purity rhSCF in large-scale.
	TO cite this article:Lili Wang,Chaozhan Wang ,Jiangfeng Liu, et al. Improve on recovery of the recombinant human stem cell factor inclusion body in refolding with simultaneous purification process for large-scale[OL].[ 6 March 2008] http://en.paper.edu.cn/en_releasepaper/content/19103


	9. The positive influences of Mg2+, Mn2+and Zn2+ addition on the gamma-CGTase Production by Bucillus macorous
	Wang feng,Du guocheng,Cheng jian
	Biology 25 October 2007
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	Abstract:The effects of Mg2+, Mn2+ or Zn2+ addition on gamma-CGTase production by Bacillus macorous WSH02-06 were studied. The gamma-CGTase yield was increased maximally almost three-times by the addition of Mg2+, Mn2+ and Zn2+. The addition of these cations also stimulated cell growth. The consumption of Mg2+ was promoted in the cultures with the co-existance of Mg2+ with Mn2+ or/and Zn2+. The maximal gamma-CGTase yield and dry cell weight showed a linear increasing relation with the consumption of Mg2+. The increased intracellular concentration of ATP and ATP-to-ADP ratios by the addition of these cations can provide more energy for the better cell growth and higher gamma-CGTTase production.
	TO cite this article:Wang feng,Du guocheng,Cheng jian. The positive influences of Mg2+, Mn2+and Zn2+ addition on the gamma-CGTase Production by Bucillus macorous[OL].[25 October 2007] http://en.paper.edu.cn/en_releasepaper/content/15921


	10. Ethanol tolerance and the variation of plasma membrane composition of yeast floc populations with different size distribution
	Juanjuan Lei,Zhao Xinqing,Ge Xumeng,Bai Fengwu
	Biology 31 May 2007
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	Abstract:The ethanol tolerance of a self-flocculating yeast strain SPSC01 was investigated in an oxygen-limited fed-batch bioreactor. Employing Focused Beam Reflectance Measurement (FBRM) on-line monitoring system, four yeast floc populations with the average size ranging from 100 to 400μm were obtained. It was found that ethanol tolerance increased with the increasing floc size in the 100, 200, and 300μm floc populations, while increasing the average floc size further to 400μm resulted in lower ethanol tolerance. Examination of the membrane composition of different floc populations revealed that the plasma membrane composition of the floc populations was significantly different in the contents of ergosterol, phosphatidylinositol, as well as phospholipid palmitoleic acid. What’s more, the plasma membrane of more ethanol tolerant floc population was less permeable when subjected to 15% (V/V) ethanol shock treatment, and the plasma membrane ATPase activities were higher in the floc populations with higher ethanol tolerance. These results indicate that the average size distribution of the floc populations exerted great influence on the physiological status of yeast cells during the ethanol production process, leading to the changes in plasma membrane composition that contributed to improved ethanol tolerance in self-flocculating yeast SPSC01.
	TO cite this article:Juanjuan Lei,Zhao Xinqing,Ge Xumeng, et al. Ethanol tolerance and the variation of plasma membrane composition of yeast floc populations with different size distribution [OL].[31 May 2007] http://en.paper.edu.cn/en_releasepaper/content/13181

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