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	1. Variation of weed community composition in late rice crops due to long-term fertilization
	Yin Lichu,Zhang Yangzhu
	Agronomy 04 March 2010
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	Abstract:The effect of various fertilization treatments on weed species composition in late rice crops was investigated in experimental plots established 26 years previously in Wangcheng county, Hunan province, China. The treatments examined influenced weed flora composition and density. Multivariate analyses showed that changes in the weed community composition were primarily due to light intensity on the field surface, followed by soil available N and P. More weed species and total weed density were observed in the control and PK plots, but less weed species and total density were found in the plots in which N, P, and K were applied together. Omission of N-application had more important influence on the weed community compared with the omission of P-, or K-application. Nutrient source from synthetic fertilizers and organic manure or additional lime application had no obvious effect on the weed community in late rice crops.
	TO cite this article:Yin Lichu,Zhang Yangzhu. Variation of weed community composition in late rice crops due to long-term fertilization[OL].[ 4 March 2010] http://en.paper.edu.cn/en_releasepaper/content/40392


	2. Changes in weed community composition of early rice crops due to long-term fertilization
	Yin Lichu,Zhang Yangzhu
	Agronomy 03 March 2010
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	Abstract:The effect of various fertilization treatments on weed species composition of early rice crops were investigated in experimental plots established 26 years previously in Wangcheng county, Hunan province, China. The treatments examined influenced weed flora composition and density. Multivariate analyses showed that changes in the weed community composition were primarily due to light intensity on the field surface, followed by soil available P and N. More weed species and total weed density were observed in the control and NK plots, but less weed species and total density were found in the plots in which N, P, and K were applied together. Omission of P-application had more important influence on the weed community compared with the omission of N-, or K-application. Nutrient source from synthetic fertilizers and organic manure or lime application had no obvious effect on the weed community.
	TO cite this article:Yin Lichu,Zhang Yangzhu. Changes in weed community composition of early rice crops due to long-term fertilization[OL].[ 3 March 2010] http://en.paper.edu.cn/en_releasepaper/content/40375

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	3. Effects of applying byproduct from flue gas desulfurization in batches on sodic soils quality and sunflower growth
	Wang Jinman
	Agronomy 11 January 2010
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	Abstract:In order to study the application mode of By-product from Flue Gas Desulfurization (BFGD) used to reclaim sodic soils, the field plot experiment of sunflower was conducted to analyze effects of two application modes, applying BFGD in batches and applying BFGD only once, on the physical and chemical properties of two sodic soils (in sodic soil I, ESP=19.8; in sodic soil II, ESP=46.9) and the sunflower production. The results indicated that ESP and pH of the top 40cm soil decreased significantly, and germination rate, dry matter weight and sunflower production increased after applying BFGD in all the treatments. The soil EC in the top 40 cm soil appreciably increased in initial stage after applying BFGD. However, it was lower after two years than that in initial stage and soil EC was less than, or close to the value before the experiment. The mode of applying BFGD in batches was better than that of applying it only once because the mode of applying BFGD in batches could decrease the accumulation of soil salts and promote the dissolution of CaSO4 .The ESP of sodic soil I and sodic soil II reduced to 13.5 and 14.9 respectively, their pH reduced to 8 and 8.4 respectively, and sunflower production increased by 1630.80 kg昲m-2 and 1315.65 kg昲m-2 respectively in the most effective treatments of applying BFGD in batches. In contrast to the mode of applying BFGD only once, the soil pH decreased by 4.76% and 2.33% in sodic soil I and sodic soil II, ESP decreased by 8.78% and 24.75%, germination rate increased by 4.71% and 17.57% and sunflower production increased by 12.95% and 17.52%.If the drainage was in good condition and the byproduct was applied at right rate, the salts of soil top layer would not be accumulated.
	TO cite this article:Wang Jinman. Effects of applying byproduct from flue gas desulfurization in batches on sodic soils quality and sunflower growth[OL].[11 January 2010] http://en.paper.edu.cn/en_releasepaper/content/38773


	4. Shifts in microbial community under different fertilizers in a lab incubation
	Zhang qichun,Guang-huo WANG
	Agronomy 05 January 2010
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	Abstract:The effects of different fertilization treatments on paddy soil microbial communities were characterized using several techniques. Treatments included no fertilizer (NF), inorganic fertilizer only (CF), pig manure in combination with inorganic fertilizer (CFM) and straw manure in combination with inorganic fertilizer (CFS). Assessments included determination of microbial population counts by soil dilution plating on various general and selective culture media, microbial activity by community level physiological profiling using Biolog sole carbon source utilization tests (Biolog) and phospholipid fatty acid (PLFA) profiles. Soil populations with organic manure tended to have higher levels of culturable bacteria and fungi and lower levels of actinomycetes. Biolog plates indicated that the soil microbial metabolism quotient (AWCD), Shannon index and McIntonsh index all increased significantly in CFS- and CFM-treated soils relative to NF-treated soil. Soil phospholipid fatty acid (PLFA) analysis demonstrated the monounsaturated fatty acids 18:1ω9c, 18:1ω7c, 16:1ω7c, 16:1ω9c, 15:1ω6c and 18:2ω6,9c were most prevalent in CFM- and CFS-treated soils. The saturated straight chain lipids 14:0, 17:0, 18:0, 19:0 and 20:0 were most enriched in NF- and CF-treated soils. Fertilization increased the PLFA biomarkers for bacteria and fungi, but reduced the markers for actinomycetes. The ratio of Gram-positive to Gram-negative bacteria was significantly higher in the NF- and CF-treated soils relative to the CFS- and CFM-treated soils (P<0.05). However, further studies are required to understand how these changes in microbial community structure might actually impact soil microbial community function.
	TO cite this article:Zhang qichun,Guang-huo WANG. Shifts in microbial community under different fertilizers in a lab incubation[OL].[ 5 January 2010] http://en.paper.edu.cn/en_releasepaper/content/38481


	5. Design, Synthesis and QSAR of Novel 4-(3-trifluoro methylphenyl)-6-methyl-2-arylpyridazin-3(2H)-ones
	zhu you-quan,hu xu hong,zhang jin,yuan yan-wei,xu han,zou xiao-mao,hu fang-zhong,yang hua-zheng
	Agronomy 29 December 2009
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	Abstract:A series of novel 4-(3-trifluoromethylphenyl)-6-methyl-2-arylpyridazin-3(2H)-ones derivatives were designed, synthesized and evaluated for their bioactivities. Some of them provided > 80 % inhibition of chlorophyll of Spirodela polyrrhiza at 10 μg/ml and and exhibited good herbicidal activities against Digitaria Adscendens at pre-emergence treatment at a dosage of 150 g/ha. Quantitative structure–activity relationship studies were performed on these compounds using physicochemical parameters (electronic, steric) as independent and chlorophyll inhibiting activity as dependent parameter, where chlorophyll inhibiting activity correlated best (r>0.8) with steric(Es ), indicator(HM), van der Waals volume (VN), electronic parameter(σm, σp) in this set of molecules.
	TO cite this article:zhu you-quan,hu xu hong,zhang jin, et al. Design, Synthesis and QSAR of Novel 4-(3-trifluoro methylphenyl)-6-methyl-2-arylpyridazin-3(2H)-ones[OL].[29 December 2009] http://en.paper.edu.cn/en_releasepaper/content/38239


	6. Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidative capacity in root tips of two wheat genotypes differing in aluminum tolerance
	Xu Fangjie,Li Gang,Jiang Sisi,Zhang Yongsong,Lin Xianyong
	Agronomy 29 December 2009
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	Abstract:
	TO cite this article:Xu Fangjie,Li Gang,Jiang Sisi, et al. Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidative capacity in root tips of two wheat genotypes differing in aluminum tolerance[OL].[29 December 2009] http://en.paper.edu.cn/en_releasepaper/content/38216


	7. Isolation and Characterization of a Phytase Gene (Sphy1) from Soybean (Glycine max (L.) Merr.)
	Guo Li,Wang Jiaojiao,Xiao Kai
	Agronomy 17 December 2009
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	Abstract:A novel phytase gene Sphy1 was isolated based on screening a cDNA library which was constructed from germinated soybean (Glycine max (L.) Merr. cv. Kefeng6) cotyledon. The full-length cDNA of Sphy1 was 1 644 bp predicated to encode 547 amino acids including an N terminal signal peptide of 27 amino acids. Phylogenetic analysis indicated that Sphy1 had high similarities with the phytase genes from M. truncatula and rice, and acid phosphatase genes from M. truncatula and Arabidopsis. Prokaryotic expression of Sphy1 in BL21 showed that the induced protein had high phytase activities. The transcripts of Sphy1 could be detected in various tissues, such as cotyledons, leaves, stems and roots of seedlings. The phytase activities in the above tissues were accordance with their corresponding Sphy1 transcripts. The transcripts of Sphy1 in cotyledons showed an increasing trend from 5 to 30 days after germination, suggesting that Sphy1 had involved the hydrolyses of the organic phosphorus compounds in seeds from the stages of early seed germination to young seedlings in soybean. Therefore, it is speculated that Sphy1 plays an important role during the seed germination and the growth of the seedlings by releasing inorganic phosphorus (Pi) from phosphorus reserve in seeds.
	TO cite this article:Guo Li,Wang Jiaojiao,Xiao Kai. Isolation and Characterization of a Phytase Gene (Sphy1) from Soybean (Glycine max (L.) Merr.)[OL].[17 December 2009] http://en.paper.edu.cn/en_releasepaper/content/37665


	8. Dynamic Monitoring and Systemic Distribution of ‘Candidatus Liberibacter asiaticus’ in Affected Plant and Insect Vector by Taqman Real-Time PCR
	Zhou Binbin,Yin Youping,Hu Hao,Wang zhongkang
	Agronomy 27 May 2009
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	Abstract:Huanglongbing(HLB) is one of the most destructive citrus diseases worldwide, in which the uncultured phloem-restricted α-proteobacterium ‘Candidatus Liberibacter’ is always associated. A Taqman real-time polymerase chain reaction (RTi-PCR) assay method specific for ‘Candidatus Liberibacter asiaticus’ (Las) has been developed previously. In this study, the RTi-PCR was used to examine and dynamic monitor the distribution of Las in the infected citrus plant and insect vector, citrus psyllid (Diaphorina citri). Quantitative analysis of Las titer indicated that it was distributed unevenly in plant and psyllid, and basically, those sections with more sieve-tube tissue had more pathogens. Dynamic analysis results showed that the titer has a significant change throughout a year in citrus leaf mid-vein and psyllid vector. Understanding the distribution and dynamic change of the bacterium inside the citrus tree and psyllid is critical for HLB research work like transmission mechanism and field epidemic regulation. The results in our study show that RTi-PCR is an excellent tool for quantitative diagnosis of HLB pathogen, as well as for monitoring the pathogen and assessing resistance and tolerance to Las in citrus breedings. Furthermore, the RTi-PCR is also an efficient technique to quantitatively evaluate the other endophytic bacteria in their host.
	TO cite this article:Zhou Binbin,Yin Youping,Hu Hao, et al. Dynamic Monitoring and Systemic Distribution of ‘Candidatus Liberibacter asiaticus’ in Affected Plant and Insect Vector by Taqman Real-Time PCR[OL].[27 May 2009] http://en.paper.edu.cn/en_releasepaper/content/32639


	9. A multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of citrus huanglongbing and citrus bacterial canker disease
	Wang zhongkang,Zhou Binbin,Hu Hao,Yin Youping
	Agronomy 27 May 2009
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	Abstract:
	TO cite this article:Wang zhongkang,Zhou Binbin,Hu Hao, et al. A multiplex polymerase chain reaction method for reliable, sensitive and simultaneous detection of citrus huanglongbing and citrus bacterial canker disease[OL].[27 May 2009] http://en.paper.edu.cn/en_releasepaper/content/32626


	10. Genetic diversity of “Candidatus Liberibacter asiaticus” isolates from different geographical regions in China
	Zhou Binbin,Yin Youping,Hu Hao,Wang Zhongkang
	Agronomy 26 May 2009
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	Abstract:Citrus Huanglongbing (HLB) is one of the most destructive diseases. HLB was demonstrated grafting transmissibility via infected citrus scion in Southern China, therefore, it is important to investigate HLB disease epidemiology. In this study, a genetic diversity study was conducted using PCR-RFLP based on the outer membrane protein gene (omp) sequences. The amplicons of “Ca. L. asiaticus” were digested with enzymes. The difference of restriction patterns show that “Ca. L. asiaticus” may comprise several different omp strains, even among strains within a given region. DNA sequences and the deduced amino acid sequences of omp gene were compared to those in GenBank database with BLAST and the results were used to construct phylogenetic tree. The omp gene of “Ca. L. asiaticus” from China were highly homogeneous, but other “Candidatus. Liberibacter” species were not introduced or developed in china so far. The result is important on explorer HLB disease epidemiology and can help the scientific community develop more efficient methods to control the diseases.
	TO cite this article:Zhou Binbin,Yin Youping,Hu Hao, et al. Genetic diversity of “Candidatus Liberibacter asiaticus” isolates from different geographical regions in China[OL].[26 May 2009] http://en.paper.edu.cn/en_releasepaper/content/32562

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