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	1. NbDREPP Contributes to Potyvirus Movement and Transports to Plasmodesmata via the Early Secretory Pathway and the Actomyosin System
	GENG Chao,CONG Qianqian,LI Xiangdong,MOU Anli,GAO Rui,LIU Jinliang,TIAN Yanping
	Agronomy 13 January 2015
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	Abstract:To identify the host factor interacting with P3N-PIPO of Tobacco vein banding mosaic virus (TVBMV), yeast two hybrid, bimolecular fluorescence complementary and co-immunoprecipitation assays were performed in this study. The host factor NbDREPP was identified to interact with TVBMV P3N-PIPO. The knockdown of NbDREPP gene expression in N. benthamiana impeded the cell-to-cell movement of TVBMV. NbDREPP was shown to co-localize with TVBMV P3N-PIPO and CI at plasmodesmata (PD) and traffick to PD via the early secretory pathway and the actomyosin motility system. And myosin XI-2 is specially required for transporting NbDREPP to PD. In conclusion, NbDREPP is a key host protein within the early secretory pathway and the actomyosin motility system that interacts with two movement proteins and influences virus movement.
	TO cite this article:GENG Chao,CONG Qianqian,LI Xiangdong, et al. NbDREPP Contributes to Potyvirus Movement and Transports to Plasmodesmata via the Early Secretory Pathway and the Actomyosin System[J].


	2. Occurrence of fruit rot caused by Ceratocystis fimbriata in Ziziphus jujube in China
	HAN Yonghua,LI Xiao,YANG Yunyu,LI Jie,XU Kecheng,ZHANG Yu,HUANG Qiong
	Agronomy 11 November 2014
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	Abstract: The fruit rot was found on the jujube on the August of 2013 in Kunming, China. The isolation was made from symptomatic tissue, and its pathogenicity was confirmed via pathology test. After the mor-phological characteristics and molecular analysis, the pathogen was identified as Ceratocystis fimbriata. This is the first report of C. fimbriata causing jujube fruit rot in China.
	TO cite this article:HAN Yonghua,LI Xiao,YANG Yunyu, et al. Occurrence of fruit rot caused by Ceratocystis fimbriata in Ziziphus jujube in China[OL].[11 November 2014] http://en.paper.edu.cn/en_releasepaper/content/4618069

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	3. The technical difficulties in agricultural soil survey and their solutions
	CAO Zhiping
	Agronomy 04 November 2014
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	Abstract:There are six major aspects of technical difficulties for soil quality survey: 1) accurate measurement of of soil fungal and bacterial biomass carbon; 2) rapid measurement of the number and biomass of various functional groups of soil animals; 3) castigation/revision of the physiological parameters for the soil food web model; 4) the in situ measurement of the respiratory intensity of soil biota; 5) the inter-conversions among soil organic carbon fractions measured by various physical, chemical and biological methods; 6) establishment of the soil organic carbon model driven by microbial functional groups. For the solutions, possible technical routes were proposed.
	TO cite this article:CAO Zhiping. The technical difficulties in agricultural soil survey and their solutions[OL].[ 4 November 2014] http://en.paper.edu.cn/en_releasepaper/content/4616967


	4. Pear 14-3-3b Gene is Regulated during Fruit Development and Involved in Response to Salicylic Acid
	SHI Haiyan,ZHANG Yuxing
	Agronomy 30 October 2014
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	Abstract:Plant 14-3-3 proteins (14-3-3s) are known to function in protein-protein interactions that mediate signal transduction pathways regulating many biological processes. The cDNA encoding putative 14-3-3 protein was isolated from pear (Pyrus pyrifolia) and designated Pp14-3-3b. Using PCR amplification technique, the genomic clone corresponding to Pp14-3-3b was isolated and shown to contain six introns. Phylogenetic analysis clearly demonstrated that Pp14-3-3b was classified into non-ε class of 14-3-3 super-families. Quantitative RT-PCR analysis indicated that the expression of Pp14-3-3b gene was developmentally regulated in fruit. Further study demonstrated that Pp14-3-3b expression was inhibited by salicylic acid (SA) in pear fruit. These data suggested that Pp14-3-3b might be involved in response to SA signaling during fruit ripening and senescence of pear.
	TO cite this article:SHI Haiyan,ZHANG Yuxing. Pear 14-3-3b Gene is Regulated during Fruit Development and Involved in Response to Salicylic Acid[OL].[30 October 2014] http://en.paper.edu.cn/en_releasepaper/content/4616104


	5. Effects of Calcium on Photosynthesis, Antioxidant System, and Chloroplast Ultrastructure in Tomato Leaves Under Low Night Temperature Stress
	LIUYufeng,ZHANG Guoxian,LI Tianlai
	Agronomy 16 October 2014
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	Abstract:The effect of exogenous CaCl2 on photosynthesis, antioxidant system, and chloroplast ultrastructure of tomato leaves under low night temperature (LNT) was investigated. Tomato seedlings were pretreated with 27 mM CaCl2, 5 mM EGTA respectively for 4 days and then exposed to LNT treatment at 6 C for 7 d. Plants treated with CaCl2 showed higher photosynthesis, increased stomatal aperture and chloroplast area, and decreased number of starch grains under LNT stress than those treated with distilled water. LNT increased the contents of superoxide radicals, hydrogen peroxide, and malondialdehyde, whereas calcium application reduced the contents of these compounds. The increase in antioxidant activities caused by LNT stress was also enhanced by the application of exogenous calcium. The opposite effects were observed with ethyleneglycol-bis-(2-aminoethyl) tetraacetic acid pretreatment. CaCl2 application improved photosynthesis in LNT-stressed plants. Such improvement was associated with increased stomatal conductance, improved antioxidant activities, and adjusted chloroplast structure. These results suggest that CaCl2 application improved photosynthesis in LNT-stressed plants. Such improvement was associated with increased stomatal conductance, improved antioxidant activities, and adjusted chloroplast structure. It provided fundamental information for further molecular mechanism research.
	TO cite this article:LIUYufeng,ZHANG Guoxian,LI Tianlai. Effects of Calcium on Photosynthesis, Antioxidant System, and Chloroplast Ultrastructure in Tomato Leaves Under Low Night Temperature Stress[OL].[16 October 2014] http://en.paper.edu.cn/en_releasepaper/content/4613944


	6. Modeling of rapeseed at maturity stage using 3D unorganized point clouds and digital images
	CHANG Tingting,WANG Xingyu,ZHAI Ruifang
	Agronomy 15 September 2014
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	Abstract:The 3D morphological expressin of a plant is important because this is the basis for building a functinonal-structural plant model to study the relationships between the structure, its environment, and its internal biology. Creating 3D plant models is often a difficult and laborious task especially to rapeseed because of its complexity and it has not been accomplished in the traditional ways. To make it easier and more natural, the integration of digital images and 3D unorganized point clouds from a digitizer provides a promising approach for rapeseed model generation. In the present study, 3D unorganized point clouds and digital images were incorporated in the generation of complex models of rapeseeds at maturity stage. Unorganized point clouds and image sequences were taken from different viewpoints using a 3D digitizer. The 3D unorganized points and image sequences were used for the automated registration of all data sets from all the viewpoints, which is pair-wise registration. Later, all the pair-wise registration parameters were used as initial transformation parameters for multiple registrations. The next procedure generated a surface model by triangulated irregular network using all the point clouds. The capabilities of our system were demonstrated through real data sets. Meanwhile, mesurements on some parameters such as branch locations, angles, lengths were implemented and compared to the parameters obtained by manual measurements. Experimental results showed that the average normal distances between the two scans were less than 0.3 mm after simultaneous registration, which indicated that the proposed methodology is effective and efficient.
	TO cite this article:CHANG Tingting,WANG Xingyu,ZHAI Ruifang. Modeling of rapeseed at maturity stage using 3D unorganized point clouds and digital images[OL].[15 September 2014] http://en.paper.edu.cn/en_releasepaper/content/4610117


	7. Abscisic acid, ethylene and antioxidative systems in rice grains in relation with grain filling subjected to postanthesis soil-drying
	ZHANG Hao,LIU Kai,WANG Zhiqin,LIU Lijun,YANG Jianchang
	Agronomy 13 August 2014
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	Abstract:This study tested the hypothesis that abscisic acid (ABA) and ethylene may mediate the effect of postanthesis soil-drying on grain filling of rice (Oryza sativa L.) through regulating antioxidative systems. Two rice cultivars were pot-grown, and three soil-drying treatments, well-watered (WW), moderately soil-drying (MD) and severely soil-drying (SD), were imposed from 9 days post anthesis until maturity. Levels of ABA and ethylene, activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), and contents of superoxide anion (O2-) and malondialdehyde (MDA) and their relationships with grain filling rate were investigated. The results showed that, when compared with the WW treatment, the MD treatment increased, whereas the SD treatment decreased, grain filling rate and activities of CAT, POD, and SOD in both superior and inferior spikelets. Both MD and SD treatments increased contents of ABA, superoxide anion and MDA in grains, with more increases under the SD than under the MD. The MD treatment decreased, whereas the SD treatment increased, ethylene evolution rate and 1-aminocycopropane-1-carboxylic acid (ACC) content. The ratio of ABA to ACC (ABA/ACC) was the highest under the MD and the lowest under the SD among the three soil-drying treatments. ABA content, activities of CAT, POD, SOD, and ABA/ACC very significantly and positively, while the levels of ethylene and ACC and contents of superoxide anion and MDA significantly and negatively, correlated with grain filling rate. Application of cobalt ion (inhibitor of ethylene synthesis) or ABA at early grain filling stage significantly decreased contents of superoxide anion and MDA, and increased activities of CAT, POD, SOD and grain filling rate. Spraying with ethephon (ethylene-releasing agent) or fluridone (inhibitor of ABA synthesis) had the opposite effect. The results suggest that an increase in ABA and decrease in ethylene in the grains under postanthesis moderate soil-drying could enhance the activities of antioxidative enzymes and reduce free radicals in grains, and therefore, accelerate the grain filling rate of rice.
	TO cite this article:ZHANG Hao,LIU Kai,WANG Zhiqin, et al. Abscisic acid, ethylene and antioxidative systems in rice grains in relation with grain filling subjected to postanthesis soil-drying[J].


	8. Alleviated aluminum toxicity by exogenous nitric oxide in wheat: toward regulating the ascorbate-glutathione cycle
	Sun Chengliang,Liu Lijuan,Yu Yan,Liu Wenjing,Lu Lingli,Jin Chongwei,Lin Xianyong
	Agronomy 23 July 2014
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	Abstract:The possible association with nitric oxide (NO) and ascorbate-glutathione (AsA-GSH) cycle in regulating aluminum (Al) tolerance of wheat was investigated using two genotypes with different Al resistance. Exposure to Al inhibited root elongation, triggered lipid peroxidation and oxidation of AsA and GSH to dehydroascorbate and glutathione disulfide, respectively, in wheat roots. Exogenous NO significantly increased endogenous NO levels, and subsequently alleviated Al-induced inhibition of root elongation and oxidation of AsA and GSH to maintain the redox molecules in reduced form in both wheat genotypes. Under Al stress, significantly increased activity and gene transcriptional level of ascorbate peroxidase, glutathione reductase, and dehydroascorbate reductase, were observed in the root tips of the Al-tolerant genotype Jian-864. NO application enhanced the activity and gene transcriptional level of all the above mentioned enzymes in both wheat genotypes. γ-Glutamylcysteine synthetase was not significantly affected by Al as well as NO, but NO treatments increased glutathione peroxidase and glutathione S-transferase activity to a greater extent than the Al-treated wheat seedlings. Proline was significantly decreased by Al, while was not affected by NO. These results clearly suggest the role of NO in providing protection against Al-induced oxidative stress which possibly through its regulation of the AsA-GSH cycle.
	TO cite this article:Sun Chengliang,Liu Lijuan,Yu Yan, et al. Alleviated aluminum toxicity by exogenous nitric oxide in wheat: toward regulating the ascorbate-glutathione cycle[OL].[23 July 2014] http://en.paper.edu.cn/en_releasepaper/content/4604889


	9. The co-involvement of light and air temperature in regulation of sex expression in monoeciuos cucumber (Cucumis sativus L.)
	Lina Wang,Zhonghai Ren,Xiufeng Wang
	Agronomy 06 July 2014
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	Abstract:The combination effects of light and air temperature, which were expressed as photothermal ratio (PTR), on the growth and sex expression of three monoecious cucumbers (Cucumis sativus L. cv. "Xintaimici", "Jinyan3" and "Jinyan 4") were studied with hydroponics in this research. The results showed that with the increasing of PTR, different growth responses were observed in the three cucumber cultivars. In contrast, high PTR (H-PTR, 0.86 mol m-2 degree-day-1) significantly increased the total number of female nodes and decreased the total number of male node of the three monoecious cucumber cultivars compared with cucumbers grown under low PTR (L-PTR, 0.64 mol m-2 degree-day-1). More photoassimilate was partitioned to fruits of three cucumber cultivars with the increasing of PTR, indicating that PTR-mediated femaleness of monoecious cucumber might be attributed to the enhancement of photoassimilate translocation from source organs to sink organs.
	TO cite this article:Lina Wang,Zhonghai Ren,Xiufeng Wang. The co-involvement of light and air temperature in regulation of sex expression in monoeciuos cucumber (Cucumis sativus L.)[J].


	10. Microarray Analysis of the Secretory Pathway Genes Response to TuMV Infection in Brassica rapa
	LI Yanxiao,ZHANG Changwei
	Agronomy 18 June 2014
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	Abstract:Previous evidence suggested the viral RNP complex consisting of the viral RNA moved to plasmodesmata via the secretory pathway. This global analysis of Arabidopsis gene expression identifys a complex array of changes impacting pathogen response. 38 secretory pathway genes are identified, including AP1, AP2, B-COP, F-COP, Sec23/24, ER-Golgi, PM2, PM and TGN/PM. The qRT-PCR is basically consistent with microarray analysis. All these results suggest viruses use the secretory pathway to accomplish the viral replication and movement. Exploring the precise function of the new genes in this analysis will offer new insights into the defense in Brassica rapa.
	TO cite this article:LI Yanxiao,ZHANG Changwei. Microarray Analysis of the Secretory Pathway Genes Response to TuMV Infection in Brassica rapa[OL].[18 June 2014] http://en.paper.edu.cn/en_releasepaper/content/4601531

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