Abstract:Ethyl methanesulfonate (EMS) is a stable and effective chemical mutagen. In this study, cucumber (Cucumis Sativus L. cv. 'Shannong No.5' ) seeds were treated by 1% EMS for 12 h, 24 h and 48 h to optimize EMS mutagenesis and determined median lethal dose of EMS (1% EMS and 24 h) for "Shannong No.5". After treated by 1% EMS for 24 h, 541 M1 plants were grown in greenhouse for phenotype investigation. The fertility of M1 cucumbers was very low, and only 79 lines produced seeds after self crossing. 60 independent M2 families comprising 600 M2 plants were investigated for phenotypic alteration, and 11 individual mutant lines were isolated into six groups: short-fruit mutants, long-fruit mutants, small-flower mutants, big-flower mutants, opposite-tendril mutants and clustered-leaf mutants. The mutation frequency was 18.3%. Two selected representatives, short-fruit mutants and opposite-tendril mutants, showed 1 : 3 of segregation ratio in M2 populations. This ratio is consistent with classic Mendelian model, indicating that the two kinds of mutants may be controlled by a single recessive gene, respectively. Long-fruit phenotype can be inherited and no segregation was observed in M3 generation, indicating that this mutant line may be homozygous.

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	1. Ethyl methanesulfonate (EMS)-mediated mutagenesis of cucumber (Cucumis Sativus L.)
	Lina Wang,Bing Zhang,Jinrui Li,Zhonghai Ren
	Agronomy 30 May 2014
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	Abstract:Ethyl methanesulfonate (EMS) is a stable and effective chemical mutagen. In this study, cucumber (Cucumis Sativus L. cv. 'Shannong No.5' ) seeds were treated by 1% EMS for 12 h, 24 h and 48 h to optimize EMS mutagenesis and determined median lethal dose of EMS (1% EMS and 24 h) for "Shannong No.5". After treated by 1% EMS for 24 h, 541 M1 plants were grown in greenhouse for phenotype investigation. The fertility of M1 cucumbers was very low, and only 79 lines produced seeds after self crossing. 60 independent M2 families comprising 600 M2 plants were investigated for phenotypic alteration, and 11 individual mutant lines were isolated into six groups: short-fruit mutants, long-fruit mutants, small-flower mutants, big-flower mutants, opposite-tendril mutants and clustered-leaf mutants. The mutation frequency was 18.3%. Two selected representatives, short-fruit mutants and opposite-tendril mutants, showed 1 : 3 of segregation ratio in M2 populations. This ratio is consistent with classic Mendelian model, indicating that the two kinds of mutants may be controlled by a single recessive gene, respectively. Long-fruit phenotype can be inherited and no segregation was observed in M3 generation, indicating that this mutant line may be homozygous.
	TO cite this article:Lina Wang,Bing Zhang,Jinrui Li, et al. Ethyl methanesulfonate (EMS)-mediated mutagenesis of cucumber (Cucumis Sativus L.)[J].


	2. Wavelet-based denoising of spectral domain for hyperspectral Images
	YANG Hao,ZHANG Dongyan,HUANG Linsheng,ZHAO Jinling
	Agronomy 28 April 2014
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	Abstract:Imaging spectroradiometers are highly susceptible to noise. Accurately quantitative processing with more high quality is obligatory before any derivative analysis, especially for precise agriculture application. Aiming at the Pushbroom Imaging Spectrometer (PIS) developed by us, a wavelet-based threshold denoising method was developed for its hyperspectral imagery data. And its capacity was evaluated through comparing with other popular denoising methods in pixel scale and in region scale. Furthermore, the method was validated in chlorophyll concentration retrieval application based on its red-edge extraction. The result revealed that the determination coefficient R2 of chlorophyll concentration retrieval model was improved from 0.586 to 0.811. It showed that the proposed denoising method allowed efficient denoising while maintaining image quality, and presented significant advantages over conventional denoising methods.
	TO cite this article:YANG Hao,ZHANG Dongyan,HUANG Linsheng, et al. Wavelet-based denoising of spectral domain for hyperspectral Images[J].

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	3. Evaluation of Genetic Diversity of 48 Potato Onion (Allium cepa var. aggregatum Don.) Germplasms Using SSR and AFLP markers
	JIN Xue,WU Fengzhi
	Agronomy 09 February 2014
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	Abstract:Simple sequence repeat (SSR) markers and amplified fragment length polymorphism (AFLP) were used to detect the genetic diversity of 48 potato onion germplasms from Heilongjiang and Jilin provinces in China. Twenty three SSR primers with rich polymorphism, stable amplification and good repeatability were screened from one hundred and one SSR primers. With these screened primers, 116 bands were identified, of which 66 bands were polymorphic. The genetic similarity was estimated with the Nei-Li coefficient, and results showed that the genetic similarity coefficients of 48 potato onion germplasms ranged from 0.61 to 0.91. Ten AFLP primers with rich polymorphism, stable amplification and good repeatability were screened from sixty four AFLP primers. With these screened primers, 470 bands were identified, of which 288 bands were polymorphic. The genetic similarity was estimated with the Nei-Li coefficient, and results showed that the genetic similarity coefficients of 48 potato onion germplasms ranged from 0.59 to 0.95. The clustering results of SSR and AFLP markers were basically identical, and both methods showed that the cluster results of potato onion obviously correlated with their geographic origins.
	TO cite this article:JIN Xue,WU Fengzhi. Evaluation of Genetic Diversity of 48 Potato Onion (Allium cepa var. aggregatum Don.) Germplasms Using SSR and AFLP markers[OL].[ 9 February 2014] http://en.paper.edu.cn/en_releasepaper/content/4583988


	4. Analysis of maize miRNA regulated by Exserohilum turcicum
	Fangli Wu,Weibo Jin
	Agronomy 25 January 2014
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	Abstract:Northern leaf blight, caused by the fungus Exserohilum turcicum (Pass.), is a major disease of maize (Zea mays L.). MicroRNAs (miRNAs) have been recently reported as gene expression regulators related to several stress responses; however, evidence of the role of miRNAs in plant response to biotic stresses is limited. In this study, the miRNA expression patterns in maize in response to E. turcicum stress were investigated using a plant miRNA microarray platform. A total of 118 miRNAs were detected in mock- and E. turcicum-inoculated leaves. Among these miRNAs, miR530, miR811, miR829, and miR845 were identified as new miRNAs in maize through a homology-based approach. In addition, four miRNAs were differentially regulated in response to E. turcicum: miR811, miR829, miR845, and miR408.
	TO cite this article:Fangli Wu,Weibo Jin. Analysis of maize miRNA regulated by Exserohilum turcicum[OL].[25 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4583982


	5. Sedum alfredii- A promising Zinc hyperaccumulator: Character, mechanism and application
	Gao Jun,Feng Ying,Zhang Min,Lu Ling-Li,Li Tingqiang,He Zhenli,Yang Xiaoe
	Agronomy 26 December 2013
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	Abstract:Backgroud :Previous research on Zinc (Zn) hyperaccumulation was mainly focused on Noccaeacaerulescens and Arabidopsis halleri. Information on other Zn hyperaccumulators, especially non-Brassicaceae, is essential to better understand Zn hyperaccumulation mechanisms and their application potential for soil remediation.. Scope: Sedum alfredii is a Crassulaceae Zn hyperaccumulator. In this paper, the hyperaccumulation and tolerance ability of sedum plants and Sedum alfredii populations were compared. The major mechanisms of Zn hyperaccumulation in S. alfredii were discussed with respect torhizosphere process, transport mechanisms, accumulation, and detoxification, as well as molecular basis. Finally, phytoextraction of Zn by S. alfredii and the feasible post-harvest treatments of shoot biomass were reviewed. Conclusion: Zinc hyperaccumulation in S. alfredii is not a species constitutive trait. Hyperaccumulating ecotype (HE) S. alfredii possesses a root foraging mechanism and an altered Zn transport system to increase root uptake and subsequent translocation to shoot. A complex interaction of microbes, plant, and environment occurs in the rhizosphere that facilitate Zn hyperaccumulation. The accumulated Zn is mainly sequestered into inactive sites. Compared with N. caerulescens and A. halleri, S. alfredi inot only provide valuable information on metal hyperaccumulation, but holds a greater promise for application in phytoextraction at a large field scale .
	TO cite this article:Gao Jun,Feng Ying,Zhang Min, et al. Sedum alfredii- A promising Zinc hyperaccumulator: Character, mechanism and application[OL].[26 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4578718


	6. Selection of suitable reference genes for gene expression studies in periwinkle infected by Wheat Blue Dwarf phytoplasma
	LI Yan,CHEN Wang,WU Yunfeng
	Agronomy 24 December 2013
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	Abstract:qRT-PCR has becoming a widely used method for detection and characterization of gene expression in recent years. However, the accuracy of the qPCR output strongly depends on the performance of selected internal reference genes. Furthermore, there are few reports about the selection of suitable reference genes in plants challenged by phytoplasma. In this study, we used periwinkle, the experimental host of phytoplasma, as material to detect the most constitutively expressed reference genes during wheat blue dwarf (WBD) phytoplasma infection. Ten candidate reference genes were selected, and their expression stability were evaluated and analyzed using the algorithms geNorm. Our results showed that the Ct values of candidate reference genes exhibited a relatively wide range among all of the experimental samples. Different suitable reference genes for normalization should be selected depending on the experimental conditions. Taken together, the set of reference genes presented here will provide a reference gene application guideline for gene expression characterization of periwinkle infected by phytoplasma.
	TO cite this article:LI Yan,CHEN Wang,WU Yunfeng. Selection of suitable reference genes for gene expression studies in periwinkle infected by Wheat Blue Dwarf phytoplasma[OL].[24 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4576095


	7. Fine mapping and candidate gene analysis of gl-1, a gene controlling trichome formation in rice
	ZENG Ruizhen,LIU Ziqiang
	Agronomy 24 December 2013
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	Abstract:Leaf trichome plays an important role in protecting plants against insect herbivores, loss of water through transpiration and UV irradiation. In our present study, SSSL-W24, a single segment substitution line (SSSL) containing only one chromosome segment of the glabrous japonica cultivar Star bonnet 99 in the genetic background of the pubescent indica cultivar HJX74 showed glabrous leaves and hulls. Genetic analysis of the F2 generation, derived from a cross between SSSL-W24 and HJX74, showed that these traits are controlled by the recessive gene gl-1, which was mapped to the short arm of chromosome 5. Fine mapping and high-resolution linkage analysis using 1585 F3 plants and markers ?anking gl-1 were carried out, and the gene was localized to a 35.9 kb region that contains seven annotated genes according to the genome sequence of japonica Nipponbare. Positional cloning of this gene will assist selection of hybrid rice, facilitate the mechanization of agriculture and increase the warehousing capacity of rice.
	TO cite this article:ZENG Ruizhen,LIU Ziqiang. Fine mapping and candidate gene analysis of gl-1, a gene controlling trichome formation in rice[OL].[24 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4577925


	8. Comparative transcript profiling of gene expression between self-incompatibility and self-compatibility mandarin by suppression subtractive hybridization and cDNA microarray
	Miao Hongxia,Ye Zixing,Hu Guibing,Qin Yonghua
	Agronomy 24 December 2013
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	Abstract:Self-incompatibility (SI) is an important trait of Citrus plants that is exploited by farmers for producing seedless fruit in Citrus. However, the molecular mechanism of SI in Citrus is not well understood. 'Wuzishatangju' (Citrus reticulata Blanco) (Self-incompatibility, SI) is an excellent seedless mandarin cultivar derived from a bud sport variation of a seedy 'Shatangju' cultivar (Self-compatibility, SC). Therefore, the two cultivars are excellent materials to study the mechanisms of SI and/or SC in Citrus. An integrative strategy combining eight suppression subtractive hybridization (SSH) libraries with cDNA microarray was used to study the different molecular mechanisms between SI 'Wuzishatangju' and SC 'Shatangju' (control) mandarins. A custom microarray screen resulted in a total of 1820 up- or down-regulated (Ratio≥3.0) obtained from 9810 positive clones. The expression of genes involved in embryonic development, ubiquitination pathway, Ca2+-signaling pathway, gibberellins (GAs) and auxin was significantly up-regulated in SI 'Wuzishatangju' compared with SC 'Shatangju' mandarin. The microarray analysis suggests that ubiquitin mediated proteolysis pathway might be involved in the SI reaction of 'Wuzishatangju.' Our research highlighted some candidate pathways that participate in the mandarin SI/SC reaction and could be beneficial for understanding the SI/SC systems evolution and breeding seedless citrus fruits in the future.
	TO cite this article:Miao Hongxia,Ye Zixing,Hu Guibing, et al. Comparative transcript profiling of gene expression between self-incompatibility and self-compatibility mandarin by suppression subtractive hybridization and cDNA microarray[OL].[24 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4575123


	9. Study of PxPPO1 expression in Plutella xylostella
	LU Yanli,LI Bin,JIN Minghui,XUE Chaobin
	Agronomy 19 December 2013
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	Abstract:Insect prophenoloxidase (PPO) is a copper-containing enzyme, playing an important role in immunity reaction. In this paper, the expression of PxPPO1 at different life stages were investigated, Semi-quantitative PCR and Real-time quantitative PCR indicated that the highest amount of PxPPO1 transcripts in eggs and the 4th instar larvae, followed by the 2nd, the 3rd instar larvae, prepupae and pupa. The ORF of PxPPO1 was cloned and inserted into pET-30a prokaryotic expression vector, the recombinant proteins was expressed in Escherichia coli, and was confirmed with an 83 kDa visible, but insoluble protein.
	TO cite this article:LU Yanli,LI Bin,JIN Minghui, et al. Study of PxPPO1 expression in Plutella xylostella[OL].[19 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4575162


	10. Sequence analysis of a plasmid from wheat blue dwarf phytoplasma
	CHEN Wang,LI Yan,LIU Shubin,WU Yunfeng
	Agronomy 17 December 2013
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	Abstract:A plasmid of wheat blue dwarf (WBD) phytoplasma, named pWBDa, were cloned. It was 3449bp in length. Sequence analysis revealed that four proteins were encoded by this plasmid, including two secreted protein and four membrane proteins. Replication-associated protein (RepA), cop number control protein (Cop) and single-stranded DNA-binding protein (SSB) were found in these four proteins.
	TO cite this article:CHEN Wang,LI Yan,LIU Shubin, et al. Sequence analysis of a plasmid from wheat blue dwarf phytoplasma[OL].[17 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4576092

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