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	1. Effect of Hot Water Treatment on the Inhibition of Anthracnose, PG, PME Activity and PGIP Gene Expression in Harvested Papaya Fruits
	Zhao Nan ,Li Xueping ,Chen Weixin ,Shi Jingying
	Agronomy 24 January 2009
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	Abstract:The effect of hot water treatment on the inhibition of anthracnose, the activity of polygalacturonase (PG) and pectin methylesterase (PME), and polygalacturonase-inhibiting protein (PGIP) gene expression in harvested papaya fruits were studied. The incidence of anthracnose of harvested papaya fruits was reduced by appropriate hot water treatment. Immerging in hot water at 54 oC for 4 min showed an obvious effect on controlling postharvest decay in papaya fruits. The fruit ripening was found to be delayed, PG and PME activities were significantly inhibited, and PGIP gene expression was enhanced in hot water-treated papaya fruits. The results suggested that hot water treatment might induce the resistance of papaya fruits to anthracnose disease and extend the storage life.
	TO cite this article:Zhao Nan ,Li Xueping ,Chen Weixin , et al. Effect of Hot Water Treatment on the Inhibition of Anthracnose, PG, PME Activity and PGIP Gene Expression in Harvested Papaya Fruits[OL].[24 January 2009] http://en.paper.edu.cn/en_releasepaper/content/28308


	2. Extraction of high quality of RNA and construction of a suppression subtractive hybridization (SSH) library from Trifoliate Orange ( Poncirus trifoliata )
	Jin-Zhi Zhang,Zhi-Min Li,Jia-Ling Yao,chun-Gen Hu
	Agronomy 21 January 2009
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	Abstract:To gain a better understanding of gene expression in early flowering trifoliate orange mutant (precocious trifoliate orange, Poncirus trifoliata), suppressive subtractive hybridization (SSH) and bioinformatics were applied to identify differentially expressed genes in precocious trifoliate orange. However, isolation of high quality RNA from buds, internodal shoots, and mature leaf tissues of trifoliate orange is difficult because of its high levels of polyphenols, polysaccharides and other secondary metabolites. An modified extraction procedure gave satisfactory results, proteinase K and high concentrations of β- mercaptoethanol were used in the extraction buffer to improve RNA quality and yeild. In addition, proteinase K involved in the procedure avoided badly injury caused by diethyl pyrocarbonate (DEPC), too. RNA isolated using above method was of sufficient quality for construction of suppression subtraction hybridization (SSH) library, which allowed the isolation of several early flowering ESTs.
	TO cite this article:Jin-Zhi Zhang,Zhi-Min Li,Jia-Ling Yao, et al. Extraction of high quality of RNA and construction of a suppression subtractive hybridization (SSH) library from Trifoliate Orange ( Poncirus trifoliata )[OL].[21 January 2009] http://en.paper.edu.cn/en_releasepaper/content/28174


	3. Isolation and Characterization of Homolog PtLEAFY promoter in Trifoliate Orange (Poncirus trifoliata L. Raf. )
	Mei Li,Zhang Jinzhi,Li Zhimin,Hu Chungen
	Agronomy 19 January 2009
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	Abstract:Abstract The long juvenile phase for 6 to 8 years in woody plants has become a serious obstacle for genetic analysis and breeding practice. In order to understand the flower transition mechanism in woody plants, DNA sequence of a LEAFY homologue in trifoliate orange (Poncirus trifoliata L. Raf.) was isolated and characterized. A chimeric expression construct PtLEAFY::GUS indicated the promoter from woody plant acted its role in Arabidopsis thiliana from 7-day old shoot apical meristem to rosette leaves. These findings concluded that PtLEAFY promoter from woody plants had immediate function in annual plants and may potential provide the specific promoter of shoot apical meristem .
	TO cite this article:Mei Li,Zhang Jinzhi,Li Zhimin, et al. Isolation and Characterization of Homolog PtLEAFY promoter in Trifoliate Orange (Poncirus trifoliata L. Raf. )[OL].[19 January 2009] http://en.paper.edu.cn/en_releasepaper/content/28042


	4. Lead Distribution in the Tissue of Camellia Sinensis (L.) Kuntze after Leaf Pollution
	Jiangyu Fang,Xiaochun Wan
	Agronomy 23 July 2008
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	Abstract:Atmospheric lead (Pb) resulting from modern developing industry enters tea plant leaves and becomes a potential danger threatening people抯 health when they drink tea. To understand the storage capacity of Pb from the air by tea leaves and the possible translocation, we measured by Atomic Absorbance Spectrometer (AAS) Pb contents in leaves, stems and roots of tea seedlings after the two sides of the blade were coated with 180 mg/L C4H12O7Pb?H2O solution for over 6 months and investigated Pb distribution at tissue level by Environmental scanning electron microscopy (ESEM) coupled with energy dispersive X-ray microanalysis (EDXA). The fact of Pb amount in leaves, stems and roots of 132.54 mg/Kg, 82.33 mg/Kg and 30.20 mg/Kg, respectively, suggests an easy Pb uptake and deposition by tea leaves, and phloem translocation. This mobility of Pb in phloem was confirmed by a serious of micro analyses. As the start of Pb uptake, the upper side of the blade lost the cell profiles while the lower kept distinct cell shapes, there was less Pb in lower epidermis near phloem than in the upper epidermis near xylem and less Pb in phloem than in xylem. Accordingly, Pb content in phloem increased from leaf to stem, then to root indicating its translocation via phloem. Relative stable Pb content in xylem tissues of the three organs further supported that the upward transport of Pb was more difficult than the downward. There was no evidence of different amount of Pb in specialized structures like trichomes or storage particles.
	TO cite this article:Jiangyu Fang,Xiaochun Wan. Lead Distribution in the Tissue of Camellia Sinensis (L.) Kuntze after Leaf Pollution[OL].[23 July 2008] http://en.paper.edu.cn/en_releasepaper/content/23029


	5. A nuclear division between mitosis and amitosis
	Li Ming-Yin
	Agronomy 11 April 2008
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	Abstract:The nuclear division, according to the appearance of chromosomes or not, would be divided into two models: indirect mitosis and direct amitosis. One division between amitosis and mitosis has not been reported. During our experiments with a mutated clone of the haploid plant “Kleiner Liebling” in Pelargonium zonale 5/74/2, a nuclear division in polyploidized epidermis cells was found that showed neither the typical amitosis characteristics nor the typical mitosis. The new division contains the constriction of the amitotic division and the visible chromosome of the mitotic division. Thus, we consider that it is a mixture of the amitosis and mitosis. It can call the m-mitosis. With the m-mitosis could explain the formation of different multiform nuclei.
	TO cite this article:Li Ming-Yin. A nuclear division between mitosis and amitosis[OL].[11 April 2008] http://en.paper.edu.cn/en_releasepaper/content/20344


	6. Isolation and Preliminary Identification of Aluminium-resistant Microorganisms from the Rhizophric Soil of Tea Plant
	Wu Ying,Liang Yuerong,Lu Jianliang,Liu Zusheng
	Agronomy 03 March 2006
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	Abstract:Microorganism isolated from rhizospheric soil of tea plant was inoculated in culture medium containing aluminium 5-20 mmol/L and an aluminium-resistant fungus ALF-1 was isolated. The ALF-1 fungus possesses aluminium-resistant ability. It could grow normally in the medium containing 20 mmol/L of aluminium and decreased the aluminium content in medium and faded the haematocylin colour of the medium. The fungus was identified as a species of Neurospora sp.
	TO cite this article:Wu Ying,Liang Yuerong,Lu Jianliang, et al. Isolation and Preliminary Identification of Aluminium-resistant Microorganisms from the Rhizophric Soil of Tea Plant[OL].[ 3 March 2006] http://en.paper.edu.cn/en_releasepaper/content/5494


	7. Factors affecting plant regeneration from cotyledon explants of cultivated pepper (Capsicum annuum L.) species
	Zhang Yuyang,Tang Yinzhu,Ye Zhibiao,Lu Yongen,Ouyang Bo,Zhang Chanjuan,Li Hanxia
	Agronomy 21 February 2006
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	Abstract:A detailed study was carried out to investigate key factors affecting bud induction and shoot elongation of pepper regeneration, and develop a reliable and highly efficient in vitro regeneration system. Ability for in vitro shoot regeneration and elongation is genotype dependent. “Chufeng” displayed the highest regeneration response: 100% frequency of adventitious bud induction with all hormone combinations tested. The fully expanded cotyledons of 8–12-day-old seedlings gave the highest regeneration efficiency. Adventitious buds subjected to 21-day-induction produced higher elongation efficiency than that of 14-day-induction. The hormone combination of Zeatin + gibberellic acid generated the most efficient bud elongation. The media for in vitro regeneration from cotyledon explants of pepper were optimized: the buds induction medium: Murashige and Skoog medium+ 6-benzyl-aminopurine 6.0 mg l–1 + indole-3-acetic acid 1.0 mg l–1; the shoots elongation medium: Murashige and Skoog salts + B5 vitamins + Zeatin 2.0 mg l–1 + gibberellic acid 1.5 mg l–1; the rooting medium: half-strength MS + 0.5 mg l–1 indole-3-butyric acid. Influence of other factors such as Ag+ and seedling nutrient juice on regeneration was also studied. In general, high frequency of regeneration was obtained from all varieties tested. Plantlets were transplanted to soil and acclimatized in the greenhouse showing normal development and grown to maturity bearing normal fruits with seeds. An overall optimized scheme for pepper regeneration in vitro from cotyledons was presented, through which 100% bud induction and 50%–100% elongation efficiency was obtained.
	TO cite this article:Zhang Yuyang,Tang Yinzhu,Ye Zhibiao, et al. Factors affecting plant regeneration from cotyledon explants of cultivated pepper (Capsicum annuum L.) species[OL].[21 February 2006] http://en.paper.edu.cn/en_releasepaper/content/5284

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