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1. The effects of luteolin on phenoloxidase and the growth of Spodotera exigua (Hübner) larvae (Lepidoptera: Noctuidae) | |||
Xue Chaobin,Luo Wanchun | |||
Agronomy 14 December 2010 | |||
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Abstract:The effects of luteolin on phenoloxidase (PO) from Spodotera exigua larvae were investigated with the use of a microtitration assay in the present paper. The results showed that luteolin could inhibit the activity of PO and concentration of this inhibitor that led to a 50% (IC50) activity reduction was estimated to be 0.47 mmol/L. The inhibitory kinetics was analyzed by Lineweaver-Burk plots and the compound was found to be a reversible competitive inhibitor with the Ki of 13.11 mmol/L. The compound was obviously toxic against the larvae (≤3rd instar) of Spodoptera exigua. The highest inhibitory percentage in the older larvae (≥3rd instar) was greater than 60% with luteolin treatment. The pupation rate and percentage of eclosion was also evidently reduced with luteolin treatment by the ingestion method in this investigation. | |||
TO cite this article:Xue Chaobin,Luo Wanchun. The effects of luteolin on phenoloxidase and the growth of Spodotera exigua (Hübner) larvae (Lepidoptera: Noctuidae)[OL].[14 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4397554 |
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