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1. Development of an indirect ELISA method for detecting antibody against Trichomonas gallinae from pigeon | |||
Luo Fong,Su Suiqin,Liang Gang,Chen Zehua,LI Yawen,LI Guoqing | |||
Animal Husbandry, Veterinary Medicine 05 May 2012 | |||
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Abstract:To develop an indirect ELISA method for detecting antibody of pigeon against T. gallinae, the optimal antigen density and positive serum dilution were determined by the chessboard test, then the specificity and reproducibility was detected, 72 positive and 45 negative serum were assayed with the indirect ELISA to assess the sensitivity and specificity and accuracy of the method. Results showed that the working concentration of antigen-antibody were optimal when the T. gallinae antigen was diluted to 5×105/mL and the positive serum was diluted to 1:400. The optimal incubation time of antigen-antibody reaction and antigen-antibody with anti-pigeon IgG:HRPO conjugate reaction was sixty minutes. The specificity, relative sensitivity, and accuracy were 86.67%, 100% and 94.87% respectively compared with wet film examination. The largest coefficient of variation of 10 serums was 11.22% in the repeatability test. The results indicate that the indirect ELISA method has good specificity, higher sensitivity and good repeatability. It is concluded that the indirect ELISA for detecting antibody against T. gallinae of pigeon was developed successfully. . | |||
TO cite this article:Luo Fong,Su Suiqin,Liang Gang, et al. Development of an indirect ELISA method for detecting antibody against Trichomonas gallinae from pigeon[OL].[ 5 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4477128 |
2. Effects of exogenous metallothionein on several endocrine hubs of dairy cattles | |||
Wu Lizhuan,Chen Haiyan,Zhang Bin,Li Lili,Xiao Bingnan,Chen Yuguang,Xiao Dingfu,Luo Jiajie,Wu Zongming | |||
Animal Husbandry, Veterinary Medicine 26 January 2010 | |||
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Abstract:To approach the effects of exogenous metallothionein (Zn-MT) on endocrine hubs of dairy cattles on the low-grade thermal stress, an experiment was conducted with 24 lactating cows, who were randomly allocated to groups A, B, C and D, and supplemented with 0, 6.0, 12.0 and 16.0mg Zn-MT•capita-1, respectively, by intravenous injection. The results showed that: The corrected milk yields in groups B, C and D were higher than those in groups A (P<0.05or P<0.01), the fat percentages of milk was improved significantly (P<0.05). The secretory capacity of INS in control group was lower than in experimental groups, especially in group B in 30d(P<0.01); the secretory capacity of IGF-Ⅰand T3 in groups B, C and D were higher than those in groups A (P<0.05or P<0.01), and in 45d, the same capacity in group C was the highest among all these numbers. The FSH in groups B and C constantly maintained low level, but the range of variation of FSH in group A was 4 times as same as in group C; The secretory capacity of Prog and E2 in experimental groups were higher than those of group A in anaphase (30~45d)(P<0.05); and the PRL in groups B, C and D were higher significantly than those in groups A (P<0.01), and the effect was faint in anaphase (P>0.05). Exogenous Zn-MT had the best effects on the thermoresistance of diary cattle 12.0mg•capita-1 by intravenous injection every 30~45 days. On one hand, the speed of catabolism and quantity of heat production in bodies were reduced by Zn-MT through adjusting secretory volume of endocrine hormone which is related to energy metabolism, on the other hand, to approach the healthful effects of thermoresistance, the gonadal hormones were controlled amplitude of variation, and on the same time, the secretory volume of PRL was improved by Zn-MT, which resulted in physiological status which was varied on balance for diary cattle. | |||
TO cite this article:Wu Lizhuan,Chen Haiyan,Zhang Bin, et al. Effects of exogenous metallothionein on several endocrine hubs of dairy cattles[OL].[26 January 2010] http://en.paper.edu.cn/en_releasepaper/content/39410 |
3. Numerical simulation of inspiratory airway in nasal cavity of Bactrian camels (Camelus bactrianus) | |||
Wang Hongju,Bai Zhongtian ,Wang Jianlin ,Gao Chengjuan | |||
Animal Husbandry, Veterinary Medicine 27 September 2008 | |||
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Abstract:To investigate the flow patterns in nasal cavity of Bactrian camel (Camelus bactrianus), we construct an anatomically accurate finite element model of its left nasal cavity from CT scans of a healthy adult nose. By numerically solving the steady-state Navier-Strokes and continuity equations, we study the flow distribution and the complete velocity field for inspiration throughout the nasal cavity under physiological flow rates of breathing. During inspiration, the highest velocity occurred along the nasal floor. | |||
TO cite this article:Wang Hongju,Bai Zhongtian ,Wang Jianlin , et al. Numerical simulation of inspiratory airway in nasal cavity of Bactrian camels (Camelus bactrianus)[OL].[27 September 2008] http://en.paper.edu.cn/en_releasepaper/content/24420 |
4. The Application and Assessment of Ketamine–Xylidinothiazoline Combinations for Anaesthesia in Rhesus Monkey | |||
xiao jianhua,Wang Hongbin | |||
Animal Husbandry, Veterinary Medicine 07 January 2008 | |||
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Abstract:To access the different effects of between ketamine and ketamine-Xylidinothiazoline in anesthesia of rhesus monkeys. 20 Rhesus Macaque were anesthesia with tow kinds methods in this experiment. The anesthesia effects were evaluated during anesthesia. At the same time the cardiovascular parameters, and respiration parameters were monitored with Datex circulation, respiration monitor and multi-functional physiologic graphed SJ-42 continuously and dynamically. The results showed that the effects of anesthesia was good, it reach the surgical anesthetic depth. The relation between analgesia, sedation and muscle relaxation were balanced. The induction time were 5.0±1.6min and the anesthesia maintained 55.3±6.6min in all. Furthermore the compound anesthetic has little effects on the physiology function of Rhesus Macaque and heart rate, blood pressure, blood oxygen saturation, body temperature, respiratory rate maintain in normal physiological range during anesthesia. It was evident that the compound anesthetic can meet the clinical needs. | |||
TO cite this article:xiao jianhua,Wang Hongbin . The Application and Assessment of Ketamine–Xylidinothiazoline Combinations for Anaesthesia in Rhesus Monkey[OL].[ 7 January 2008] http://en.paper.edu.cn/en_releasepaper/content/17840 |
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