Abstract:Yu-Ping-Feng is a well-known traditional Chinese multi herbal formula, and it is frequently being used to treat cold and flu and inflammation-associated diseases. We aim to evaluate the immunostimulatory activity of polysaccharide from Yu-Ping-Feng(YPF-PS) in vitro on chicken macrophage to make clear the bioactive molecular of Yu-Ping-Feng. Gel permeation chromatography (GPC) and gas chromatography?mass spectrometry (GC-MS) analysis, amino acid analysis and nuclear magnetic resonance (NRM) spectroscopy elucidated the structure of the YPF-PS. In order to evaluate the biological activities of YPF-PS, lipopolysaccharides (LPS)-induced cell proliferation and phagocytosis rate and the levels of cytokine: nitric oxide (NO), tumor necrosis factor alpha (TNF-α), interleukin-1 beta, interleukin-6 (IL-1β, IL-6), interferon beta (IFN-β) and costimulatory molecules CD80, CD86 in chicken macrophage were detected. The results indicated that molecular weight the weight of YPF-PS was distributed on 5 KD~500 KDa, which is a mixture of glucose (Glu), fructose (Fru), arabinose (Ara) and few rhamnose with the molar ratio of 61.29: 55.47: 99.67: 19.58, having anomeric carbon signals of β-L-arabino furanoside, α-L-rhamnoside, α-D-glucopyranoside and β-D-fructofuranose. Furthermore, cell immunology experiments show that YPF-PS at dosage of 15.62 μgmL -1 could increase the LPS-induced cell proliferation and phagocytosis rate significantly in chicken macrophage, moreover, the levels of cytokine: NO, TNF-α, IL-1β, IL-6 and IFN-βin cells elevated obviously, equally shocking, costimulatory molecules CD80, CD86 were detected having a tremendous enhancement. We believe that the YPF-PS has potent immunomodulatory properties and could be explored as a novel potential immunostimulant for the food and pharmaceutical purpose.

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.


	1. Isolation, Molecules characterization and Immunostimulatory activity of polysaccharide from Yu-Ping-Feng
	ZHAO Xiaona
	Animal Husbandry, Veterinary Medicine 05 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Yu-Ping-Feng is a well-known traditional Chinese multi herbal formula, and it is frequently being used to treat cold and flu and inflammation-associated diseases. We aim to evaluate the immunostimulatory activity of polysaccharide from Yu-Ping-Feng(YPF-PS) in vitro on chicken macrophage to make clear the bioactive molecular of Yu-Ping-Feng. Gel permeation chromatography (GPC) and gas chromatography?mass spectrometry (GC-MS) analysis, amino acid analysis and nuclear magnetic resonance (NRM) spectroscopy elucidated the structure of the YPF-PS. In order to evaluate the biological activities of YPF-PS, lipopolysaccharides (LPS)-induced cell proliferation and phagocytosis rate and the levels of cytokine: nitric oxide (NO), tumor necrosis factor alpha (TNF-α), interleukin-1 beta, interleukin-6 (IL-1β, IL-6), interferon beta (IFN-β) and costimulatory molecules CD80, CD86 in chicken macrophage were detected. The results indicated that molecular weight the weight of YPF-PS was distributed on 5 KD~500 KDa, which is a mixture of glucose (Glu), fructose (Fru), arabinose (Ara) and few rhamnose with the molar ratio of 61.29: 55.47: 99.67: 19.58, having anomeric carbon signals of β-L-arabino furanoside, α-L-rhamnoside, α-D-glucopyranoside and β-D-fructofuranose. Furthermore, cell immunology experiments show that YPF-PS at dosage of 15.62 μgmL -1 could increase the LPS-induced cell proliferation and phagocytosis rate significantly in chicken macrophage, moreover, the levels of cytokine: NO, TNF-α, IL-1β, IL-6 and IFN-βin cells elevated obviously, equally shocking, costimulatory molecules CD80, CD86 were detected having a tremendous enhancement. We believe that the YPF-PS has potent immunomodulatory properties and could be explored as a novel potential immunostimulant for the food and pharmaceutical purpose.
	TO cite this article:ZHAO Xiaona. Isolation, Molecules characterization and Immunostimulatory activity of polysaccharide from Yu-Ping-Feng[OL].[ 5 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4686482


	2. Effect of dietary nitrate dosage on gas production in vitro and rumen bacterial abundance of Hu Sheep
	Chen Zhiyuan,Ma Tingting,Gong Xiaoxiao,Su Xiaoshuang,Li Guodong,Zhao Guoqi,Lin Miao
	Animal Husbandry, Veterinary Medicine 24 August 2015
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:The objective of this study was to evaluate the effect of diet supplementation with potassium nitrate on rumen gas production in vitro and bacterial diversity in vivo in in sheep. First, rumen fluid was obtained from 3 cannulated Hu sheep fed basal diet to compare gas production of in vitro diet contained nitrate 0%(A, control group), 1% (B), 2% (C), 3% (D), 4% (E), 5% (F), respectively. In a second experiment, 3 cannulated sheep were fed the six diets above, with nitrate concentration increased gradually. Each period had 16 days; before morning feeding at the end of each period, rumen fluid was collected for DNA extraction, 16S rRNA Illumina sequencing and qPCR analysis. In vitro study showed that there was no significant difference in the net gas production, theoretical maximum gas volume and speed of gas production among groups, however, the net gas production and theoretical maximum gas volume of 5% group tended to declined(P<0.1). Paired-End sequencing of 16S rRNA gene targeting showed that Chao1 index of 5% group was significantly higher than control. No difference in Shannon index was observed. At the phylum level, the total percentage of Bacteroidetes, Firmicutes, Proteobacteria, Fibrobacteres, Verrucomicrobia was more than 90%. At the genus level, Prevotella, Selenomonas, Succinivibrio were dominant genus in each group, the proportion was 47.87%, 5.76%, 2.37% respectively. qPCR showed increase in the relative abundance of Campylobacter fetus with nitrate concentration increased (P<0.01). These results indicate that nitrate addition ranged from 0% to 5% change the abundance of bacteria, especially nitrate reduction bacteria, but do not change gas production, rumen bacterial diversity and advantages community structure.
	TO cite this article:Chen Zhiyuan,Ma Tingting,Gong Xiaoxiao, et al. Effect of dietary nitrate dosage on gas production in vitro and rumen bacterial abundance of Hu Sheep[OL].[24 August 2015] http://en.paper.edu.cn/en_releasepaper/content/4652935

Saved Papers

Saved Papers


	3. Selenium-Enriched Probiotics Improves Antioxidant Status, Immune Function and Selenoprotein Gene Expression of Piglets Raised under High Ambient Temperature
	Gan Fang,Chen Xingxiang,Shengfa F. Liao,Lv Chenhui,Ren Fei,Ye Gengping,Pan Cuiling,Huang Da,Shi Jun,Shi Xiuli,Zhou Hong,Huang Kehe
	Animal Husbandry, Veterinary Medicine 04 May 2014
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:This research was conducted to evaluate the effects of selenium-enriched probiotics (SP) on growth performance, antioxidant status, immune function and selenoprotein gene expression of piglets under natural high ambient temperature in summer. Forty-eight crossbred weanling piglets randomly allocated to four groups were fed for 42 days ad libitum a basal diet without (Con, 0.16 mg Se/kg) and with supplementation of probiotics (P, 0.16 mg Se/kg), sodium selenite (SS, 0.46 mg Se/kg) and SP (0.46 mg Se/kg). From each group, three piglets were randomly selected for blood collection on Days 0, 14, 28 and 42 and tissue collection on Day 42. The SP improved growth performance of piglets. Both SS and SP increased blood glutathione peroxidase activity and tissue thioredoxin reductase 1 mRNA expression with the SP being higher than the SS. All P, SS and SP supplementation increased the superoxide dismutase activity (40.1%, 53.0% and 64.5%), glutathione content (84.6%, 104% and 165%), TCR-induced T lymphocyte proliferation (20.8%, 26.4% and 50.0%), IL-2 concentration (24.9%, 27.2% and 46.2%), and decreased malondialdehyde content (25.1%, 26.3% and 49.3%), respectively. The greatest effects of SP supplementation suggest that SP may serve as a better feed additive than P or SS for piglets under high-temperature environments.
	TO cite this article:Gan Fang,Chen Xingxiang,Shengfa F. Liao, et al. Selenium-Enriched Probiotics Improves Antioxidant Status, Immune Function and Selenoprotein Gene Expression of Piglets Raised under High Ambient Temperature[OL].[ 4 May 2014] http://en.paper.edu.cn/en_releasepaper/content/4595834


	4. miRNA profiling in the sexually mature porcine testes determined by Solexa deep sequencing
	Zhou Jiawei,Liu Gang,Guan Kaifeng,Ma Changping,Lei Bin,Tong Keya,Li Jialian,Li Fenge
	Animal Husbandry, Veterinary Medicine 03 April 2013
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:miRNAs are a large family of small regulatory elements that direct messenger RNA degradation or disrupt mRNA translation. High-throughput Solexa sequencing approach was adopted to expand the repertoire of porcine miRNAs and to explore their roles on spermatogenesis. 237,590 total reads (792 unique reads) representing 65 known mature miRNAs in the miRBase release 14.0 were detected. 116 miRNAs including 12 known miRNAs in the miRBase release 15.0 and 104 novel miRNAs were identified from the un-annotated small RNAs. 466,757 targets for 104 novel miRNAs were predicted by MIREAP. The highly expressed miRNAs in porcine testes (let-7c, let-7f, miR-148a, miR-140* and miR-199b) had a very high rate of SNP mutation, and the most popular SNP was T to G mutation at position 6. Three known miRNAs including miR-514, miR-507, miR-508-5p were significantly differentially expressed between some groups (p<0.05), while two novel miRNAs- DB-m0102 and DB-m0071 were not significantly differentially expressed between all detected groups.
	TO cite this article:Zhou Jiawei,Liu Gang,Guan Kaifeng, et al. miRNA profiling in the sexually mature porcine testes determined by Solexa deep sequencing[OL].[ 3 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4535593


	5. Zinc Prevents Salmonella enterica Serovar Typhimurium-Induced Loss of Intestinal Mucosal Barrier Function in broiler chickens
	Zhang Bingkun,Shao Yuxin,Guo Yuming,Yuan Jianmin
	Animal Husbandry, Veterinary Medicine 26 April 2012
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:The present study was carried out to evaluate the beneficial effects of supplemental zinc (Zn) on the intestinal mucosal barrier function in Salmonella enterica Serovar Typhimurium-challenged broiler chickens in a 42-d experiment. A total of 336 1-day-old male Arbor Acres broiler chicks were assigned to 8 treatment groups. A 4 × 2 factorial arrangement of treatments was used in a completely randomized experimental design to study the effects of levels of supplemental Zn (0, 40, 80, 120 mg/kg of diet), pathogen challenge (with or without S. Typhimurium challenge), and their interactions. S. Typhimurium infection caused reduction of growth performance (P <0.05) and intestinal injury as determined by reduced (P <0.05) villus height/crypt depth ratio and sucrase activity in the ileum, increased (P <0.05) plasma endotoxin levels, and reduced (P <0.05) claudin-1, occludin and MUC2 mRNA expression in the ileum at day 21. Zinc pretreatment tended to improve body weight gain (P = 0.072) in the starter period, increase the activity of ileal sucrase (P=0.077), but tended to reduce plasma endotoxin levels (P=0.080), and significantly increased (P <0.05) villus height/crypt depth ratio and mRNA levels of occludin and claudin-1 in the ileum at day 21. The results indicated that dietary Zn supplementation appeared to alleviate the loss of intestinal mucosal barrier function induced by S. Typhimurium challenge and the partial mechanism might be related to the increased expression of occludin and claudin-1 in broiler chickens.
	TO cite this article:Zhang Bingkun,Shao Yuxin,Guo Yuming, et al. Zinc Prevents Salmonella enterica Serovar Typhimurium-Induced Loss of Intestinal Mucosal Barrier Function in broiler chickens[J].


	6. Differential expression patterns of Toll-like receptor 4 at the maternal-fetal interface of pregnant Yorkshire and Meishan pigs
	Liu Huazhen,Zhang Gaoying,Peng Kemei,Liu Yinxue,Yu Mei
	Animal Husbandry, Veterinary Medicine 31 December 2011
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Toll-like receptor 4 (TLR4) has been suggested to play a very important role in modulation of immunological tolerance in the human female reproductive tract. To better understanding of functions of TLR4 in establishment and maintenance of pregnancy in pigs, the study was performed to compare the expression patterns of TLR4 at maternal-fetal interface of pregnant Yorkshire and Meishan pigs. The uteri from Yorkshire and Meishan gilts on days 26 and 50 of gestation were obtained respectively. Immunohistochemical analysis indicated that TLR4 was detected in uterine epithelium and trophoblast of these two types of pigs on days 26 and 50 of gestation. In Yorkshire gilts, the expression of TLR4 was higher in uterine epithelium than that in trophoblast on day 26 of gestation, while it was lower in uterine epithelium than that in trophoblast on day 50 of gestation. In Meishan pigs, the expression of TLR4 in trophoblast was higher than that in uterine epithelium on either day 26 or day 50 of gestation and TLR4 was found to be mainly present in the cells located at the bottom of trophoblast on day 50 of gestation. Compared with Meishan pigs, TLR4 was abundant in uterine epithelium of Yorkshire pigs on days 26 and 50 of gestation, yet it was decreased in trophoblast of Yorkshire pigs on day 50 of gestation. The results indicated that TLR4 at the maternal-fetal interface is expressed in differential patterns between Yorkshire and Meishan pigs and provided novel information about the functions of TLR4 in pig pregnancy.
	TO cite this article:Liu Huazhen,Zhang Gaoying,Peng Kemei, et al. Differential expression patterns of Toll-like receptor 4 at the maternal-fetal interface of pregnant Yorkshire and Meishan pigs[OL].[31 December 2011] http://en.paper.edu.cn/en_releasepaper/content/4458651


	7. Paradigm of Time-sequence Development of the Intestine of Suckling Piglets with microarray
	SUN Yunzi,YU Bing,ZHANG Keying,HE Jun,CHEN Xijian,CHEN Daiwen
	Animal Husbandry, Veterinary Medicine 22 February 2011
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:The interaction of the genes involved in intestinal development is the molecular basis of the regulatory mechanisms of intestinal development. The objective of this study was to identify the significant pathways and key genes that regulate intestinal development in Landrace piglets, and then to elucidate their rules of operation. The differential expression of genes related to intestinal development during suckling was investigated using a porcine genome array. Time sequence profiles were then analyzed for the differentially expressed genes to obtain significant expression profiles. Subsequently, the most significant profiles were assayed using Gene Ontology categories, pathway analysis, network analysis, and analysis of gene co-expression to unveil the main biological processes, the significant pathways, and the effective genes, respectively. In addition, quantitative real-time PCR was carried out to verify the reliability of the results of the analysis of the array. The results showed that more than 8000 differential expression transcripts were identified using microarray technology. Among the 30 significant model profiles obtained, profiles 66 and 13 were the most significant. Analysis of profiles 66 and 13 indicated that they were mainly involved in immunity, metabolism, and cell division or proliferation. Among the most effective genes in these two profiles, CN161469, which is similar to methylcrotonoyl-Coenzyme A carboxylase 2 (beta), and U89949.1, which encodes a folate binding protein, had a crucial influence on the co-expression network.
	TO cite this article:SUN Yunzi,YU Bing,ZHANG Keying, et al. Paradigm of Time-sequence Development of the Intestine of Suckling Piglets with microarray[OL].[22 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4411626


	8. Screening and Identification of Differentially Expressed Genes in Goose Hepatocytes Exposed to Free Fatty Acid
	Pan Zhixiong,Wang Jiwen
	Animal Husbandry, Veterinary Medicine 19 November 2010
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:The overaccumulation of triglycerides (TG) in hepatocytes induced hepatic steatosis, however, little is known about the mechanism of goose hepatic steatosis. The aim of this study was to define an experimental model of hepatocellular steatosis with TG overaccumulation and minimal cytotoxicity, using a mixture of various proportions of oleate and palmitate free fatty acids (FFAs) to induce fat-overloading, then using suppressive subtractive hybridization (SSH) and a quantitative PCR approach to identify genes with higher or lower expression levels after the treatment of cells with FFA mixtures as compared with untreated cells. Overall, 502 differentially expressed clones, representing 21 novel genes and 87 known genes, were detected by SSH. Based on functional clustering, up- and down-regulated genes were mostly related to carbohydrate and lipid metabolism, enzyme activity and signal transduction. The expression of 20 selected clones involved with carbohydrate and lipid metabolism pathways was further studied by quantitative PCR. The data indicated that six clones similar to the genes ChREBP, FoxO1, apoB, IHPK2, KIF1B and FSP27, which participate in de novo synthesis of fatty acid and secretion of very low density lipoproteins (VLDLs), had significantly lower expression levels in the hepatocytes of treated with FFA mixtures. Meanwhile, thirteen clones similar to the genes DGAT-1, ACSL1, DHRS7, PPARα, L-FABP, DGAT-2, PCK, ACSL3, CPT-1, A-FABP, PPARβ, MAT and ALDOB had significantly higher expression levels in the hepatocytes of treated with FFA mixtures. These results suggest that several metabolic pathways are altered in goose hepatocytes, which may be useful for further research into the molecular mechanism of goose hepatic steatosis.
	TO cite this article:Pan Zhixiong,Wang Jiwen. Screening and Identification of Differentially Expressed Genes in Goose Hepatocytes Exposed to Free Fatty Acid[OL].[19 November 2010] http://en.paper.edu.cn/en_releasepaper/content/4392163

	Check out RSS, or use RSS reader to subscribe this item