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1. Characterization and SNP Identification of Goat TYRP1 Gene | |||
Zheng Huiqin ,Lanhui Li,Xianglong Li,Rongyan Zhou,Fengling Xuan,Jingjing Zhang,Zunan Shen,Hongbo Zhao | |||
Animal Husbandry, Veterinary Medicine 20 April 2010 | |||
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Abstract:As one member of tyrosinase-related family, tyrosinase-related protein-1(TYRP1) has been reported to play an important role in melanin biosynthetic pathway in some species, but little is known about it in goat. In this study, almost complete goat TYRP1 gene (17554bp) encompassing complete CDS was determined, the coding region was 1614bp, distributed in 7 exons (2-8), intron 5 was found to possess GC-AG as its splice sites. Dozens of SNPs as well as simple sequence repeats (SSR) motifs were detected in the goat TYRP1 gene. Popgene analysis suggested that allele A at site 1263 and haplotype AC for g.1263A>C and g.1428C>T should be in favor of eumelanin biosynthesis, while mutation C→T at site 1428 occurred more recently in evolution and might have little effect on coat color. High deficiency of heterozygotes existed in Nanjiang Yellow Goat Black strain, Nanjiang Yellow Goat Fast Grow strain and Jining Gray Goat, indicating high inbreeding coefficient. Genetic differentiation among the 9 goat populations were significantly low for both g.1263A>C and g.1428C>T. | |||
TO cite this article:Zheng Huiqin ,Lanhui Li,Xianglong Li, et al. Characterization and SNP Identification of Goat TYRP1 Gene[OL].[20 April 2010] http://en.paper.edu.cn/en_releasepaper/content/42134 |
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