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	1. Analysis of Ghrelin and growth hormone secretagogue receptor expressions in mouse thymic epithelial cells
	LIU Jianhong,MA Yongjiang,LI Ying,ZHANG Yuan,LI Yugu
	Animal Husbandry, Veterinary Medicine 14 December 2012
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	Abstract:The present study was to identified the expression of Ghrelin and GHS-R in mouse thymic medullary-type epithelial cells (MTEC1).. Whether the gene of Ghrelin and GHS-R are expressing in MTEC1 cells or not was measured by using quantitative real time RT-PCR (Q-PCR). Results identified that the mRNA of Ghrelin and GHS-R can be detected in MTEC1 cells. The protein levels of GHS-R and ghrelin precursor were detected by the method of western blot analysis. Moreover, the location of GHS-R in MTEC1 cells were detected by immunofluorescence and the quantity of ghrelin in the supernatant of MTEC1 cells were measured by ELISA. The results of western blot analysis make sure that GHS-R and precursor of ghrelin are expressing in MTEC1 cells. GHS-R is a kind of transmembranes protein. as the results of immunofluorescence shown The quantity of ghrelin in the supernatant of MTEC1 cells measured by ELISA was 39.20 pg/ml.
	TO cite this article:LIU Jianhong,MA Yongjiang,LI Ying, et al. Analysis of Ghrelin and growth hormone secretagogue receptor expressions in mouse thymic epithelial cells[OL].[14 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4504387


	2. Changes in miRNAs expression profiling in testis of mice exposure to Sulfur Dioxide and Fluoride
	ZHANG Jianhai,LIANG Chen,NIU Ruiyan,SUN Zilong,WANG Jundong
	Animal Husbandry, Veterinary Medicine 12 December 2012
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	Abstract:MicroRNAs (miRNAs) are believed to play important roles in developmental and other cellular processes by hybridizing to complementary target mRNA transcripts, also involved in the development of mammalian spermatogenesis. Fluoride and sulfur dioxide are two well known environment pollutants, which present a serious threat to male reproduction. To compare the global miRNA expression profiling and regulation pattern in mouse testes between normal mouse and F or SO2-treat mice by using miRNA microarray technology, 144 male Kunming mice were divided randomly into four groups of thirty-six mice each. One group was left untreated as controls, and the other three groups were administered, respectively, for thirteen consecutive weeks, 150 mg NaF/L (45 mg F-/L) in their drinking water, SO2 in ambient air (26.2 mg/m3 SO2, 3hr/day), or were exposed to both NaF and SO2 together. After 15 weeks, testis totals RNA of the mouse model were gathered for miRNA microarray study. Simultaneity, reproductive capacity of mice, DNA contents distribution in testis cells, and bcl-2, bax, and p53 genes mRNA expression were detected to interpret the changed miRNA expression profiles. The results exhibited that MiR-106a in the NaF group, miR-449a, miR-16, miR-15b, and miR-34c in the SO2 groups, and miR-204 and miR-34a in the NaF+SO2 group were un-regulated significantly in testes of mice, compared with the controls, respectively. Whereas miR-34a decreased by 3.3-fold in the SO2 group, and miR-34b was down-regulated by more than 1.5-fold in the NaF+SO2 group. Further investigation indicates that predicted pathways according to significant miRNA are primary related to cell cycle, apoptosis, and MAPK signal pathway. Furthermore, the development of testis and reproductive capacity of male mouse have been weakened by F or SO2, especially, by F and SO2 together treatment. FCM analysis show that the percents of apoptosis cells in testis were increased significantly in experiment groups, DNA contents distributions have been disturbed by F or SO2. Expression of p53 and bcl-2 mRNA decreased, and bax gene was increased by1.87, 2.40 and 2.89 times as high as those in the control groups by QRT-PCR. However, both F or SO2 and the combination heighten the ratios of p53/bcl-2 and bax/bcl-2, all these changes lead to a common result of the spermatogenic cell apoptosis. In conclusion, changed miRNAs expression regulation should be look as the main mechanism of depressed male reproduction function of mouse induced by F or SO2 or their combination. This is the first miRNA microarray study to focus on evaluating altered miRNA expression profiles in testis of mice exposure to F or SO2. The miRNA microarray and bioinformatics analysis is a fast, high-throughout, exacts, foreground technique contribute toward elucidating the function of miRNAs in normal and impaired male reproduction by environment factors.
	TO cite this article:ZHANG Jianhai,LIANG Chen,NIU Ruiyan, et al. Changes in miRNAs expression profiling in testis of mice exposure to Sulfur Dioxide and Fluoride[OL].[12 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4501514


	3. Development of an indirect ELISA method for detecting antibody against Trichomonas gallinae from pigeon
	Luo Fong,Su Suiqin,Liang Gang,Chen Zehua,LI Yawen,LI Guoqing
	Animal Husbandry, Veterinary Medicine 05 May 2012
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	Abstract:To develop an indirect ELISA method for detecting antibody of pigeon against T. gallinae， the optimal antigen density and positive serum dilution were determined by the chessboard test, then the specificity and reproducibility was detected, 72 positive and 45 negative serum were assayed with the indirect ELISA to assess the sensitivity and specificity and accuracy of the method. Results showed that the working concentration of antigen-antibody were optimal when the T. gallinae antigen was diluted to 5×105/mL and the positive serum was diluted to 1:400. The optimal incubation time of antigen-antibody reaction and antigen-antibody with anti-pigeon IgG:HRPO conjugate reaction was sixty minutes. The specificity, relative sensitivity, and accuracy were 86.67%, 100% and 94.87% respectively compared with wet film examination. The largest coefficient of variation of 10 serums was 11.22% in the repeatability test. The results indicate that the indirect ELISA method has good specificity, higher sensitivity and good repeatability. It is concluded that the indirect ELISA for detecting antibody against T. gallinae of pigeon was developed successfully. .
	TO cite this article:Luo Fong,Su Suiqin,Liang Gang, et al. Development of an indirect ELISA method for detecting antibody against Trichomonas gallinae from pigeon[OL].[ 5 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4477128


	4. Differential expression patterns of Toll-like receptor 4 at the maternal-fetal interface of pregnant Yorkshire and Meishan pigs
	Liu Huazhen,Zhang Gaoying,Peng Kemei,Liu Yinxue,Yu Mei
	Animal Husbandry, Veterinary Medicine 31 December 2011
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	Abstract:Toll-like receptor 4 (TLR4) has been suggested to play a very important role in modulation of immunological tolerance in the human female reproductive tract. To better understanding of functions of TLR4 in establishment and maintenance of pregnancy in pigs, the study was performed to compare the expression patterns of TLR4 at maternal-fetal interface of pregnant Yorkshire and Meishan pigs. The uteri from Yorkshire and Meishan gilts on days 26 and 50 of gestation were obtained respectively. Immunohistochemical analysis indicated that TLR4 was detected in uterine epithelium and trophoblast of these two types of pigs on days 26 and 50 of gestation. In Yorkshire gilts, the expression of TLR4 was higher in uterine epithelium than that in trophoblast on day 26 of gestation, while it was lower in uterine epithelium than that in trophoblast on day 50 of gestation. In Meishan pigs, the expression of TLR4 in trophoblast was higher than that in uterine epithelium on either day 26 or day 50 of gestation and TLR4 was found to be mainly present in the cells located at the bottom of trophoblast on day 50 of gestation. Compared with Meishan pigs, TLR4 was abundant in uterine epithelium of Yorkshire pigs on days 26 and 50 of gestation, yet it was decreased in trophoblast of Yorkshire pigs on day 50 of gestation. The results indicated that TLR4 at the maternal-fetal interface is expressed in differential patterns between Yorkshire and Meishan pigs and provided novel information about the functions of TLR4 in pig pregnancy.
	TO cite this article:Liu Huazhen,Zhang Gaoying,Peng Kemei, et al. Differential expression patterns of Toll-like receptor 4 at the maternal-fetal interface of pregnant Yorkshire and Meishan pigs[OL].[31 December 2011] http://en.paper.edu.cn/en_releasepaper/content/4458651


	5. The Application and Assessment of Ketamine–Xylidinothiazoline Combinations for Anaesthesia in Rhesus Monkey
	xiao jianhua,Wang Hongbin
	Animal Husbandry, Veterinary Medicine 07 January 2008
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	Abstract:To access the different effects of between ketamine and ketamine-Xylidinothiazoline in anesthesia of rhesus monkeys. 20 Rhesus Macaque were anesthesia with tow kinds methods in this experiment. The anesthesia effects were evaluated during anesthesia. At the same time the cardiovascular parameters, and respiration parameters were monitored with Datex circulation, respiration monitor and multi-functional physiologic graphed SJ-42 continuously and dynamically. The results showed that the effects of anesthesia was good, it reach the surgical anesthetic depth. The relation between analgesia, sedation and muscle relaxation were balanced. The induction time were 5.0±1.6min and the anesthesia maintained 55.3±6.6min in all. Furthermore the compound anesthetic has little effects on the physiology function of Rhesus Macaque and heart rate, blood pressure, blood oxygen saturation, body temperature, respiratory rate maintain in normal physiological range during anesthesia. It was evident that the compound anesthetic can meet the clinical needs.
	TO cite this article:xiao jianhua,Wang Hongbin . The Application and Assessment of Ketamine–Xylidinothiazoline Combinations for Anaesthesia in Rhesus Monkey[OL].[ 7 January 2008] http://en.paper.edu.cn/en_releasepaper/content/17840

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