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There are 14 papers published in subject: since this site started. |
Results per page: | 14 Total, 2 Pages | << First < Previous 1 2 |
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1. Expression Characteristics of vasa Gene at RNA and Protein Level in Apostichopus japonicus During Gametogenesis | |||
YAN Meng,SUI Juan,SHAO Mingyu,SHENG Wanqiang,ZHANG Zhifeng | |||
Aquatic Science 17 January 2012 | |||
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Abstract:The expression product of vasa gene is a widely conserved germ line marker and participates in the development regulation of reproductive system. However, no information on vasa gene in Holothuroidea is available. In this paper, we showed the expression characteristics of Apostichopus japonicus vasa gene (Aj-vasa) during gametogenesis in ovary and testis, using in situ hybridization and immunohistochemistry techniques. The expression of Aj-vasa RNA is strongest in early germ cells (oogoniums and spermatogonia), decreases as the maturation proceeded (oocyte and spermatocyte), and absents in spermatid and sperms. Our findings show that the overall expression trend of Aj-vasa RNA is similar with that of its protein(Aj-VASA) during gametogenesis. Nevertheless there was a sexuality difference about the subcellular location of vasa RNA and its protein which was reflected in the specifically expression in cytoplasm of female germ cells and the widely expression in both nucleus cytoplasm of male germ cells. It suggested that Aj-vasa RNA as well as Aj-VASA could be the marker of germ cells during gametogenesis. | |||
TO cite this article:YAN Meng,SUI Juan,SHAO Mingyu, et al. Expression Characteristics of vasa Gene at RNA and Protein Level in Apostichopus japonicus During Gametogenesis[OL].[17 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4462753 |
2. Effects of replacement of dietary fish oil with soybean oil on growth performance and tissue fatty acid composition in marine herbivorous teleost Siganus canaliculatus | |||
LI Yuanyou,XU Shude | |||
Aquatic Science 27 July 2011 | |||
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Abstract:Fish oil (FO) substitution has been studied in many marine carnivorous fish but seldom in marine herbivorous or omnivorous species. In order to evaluate the feasibility of using soybean oil (SO) as a dietary lipid and confirm its capability of converting C18 polyunsaturated fatty acid (PUFA) into long chain polyunsaturated fatty acid (LC-PUFA) in the marine herbivorous teleost Siganus canaliculatus, juvenile fish were fed with four formulated diets differing in lipid composition, with SO accounting for 0.76% (SO0), 23% (SO23), 45% (SO45) and 67% (SO67) of total dietary lipid, respectively. After feeding for eight weeks, growth performance including weight gain, specific growth rate, feed conversion ratio and protein efficiency rate were better in the SO23 and, especially, SO45 groups than in the SO0 and SO67 groups (P < 0.05). Tissue fatty acid compositions were affected by diet, with the liver contents of eicosapentaenoic (EPA), docosapentaenoic (DPA), docosahexaenoic (DHA) acids and total n-3 PUFA displaying parallel changes with the corresponding dietary fatty acids. While the muscle contents of EPA, DPA and total n-3 PUFA between SO0 and SO23 groups, and the liver contents of arachidonic acid (ARA) and 20:4n-3, as well as the muscle content of 20:3n-6 between SO0 and SO45 groups showed no difference, confirming the biosynthesis of LC-PUFA from C18 precursors in vivo since the contents of corresponding fatty acids in diets SO23/SO45 were much lower than those in diet SO0 (P < 0.05). The results indicate that SO may be a suitable dietary lipid source for S. canaliculatus, and can replace up to 67% or 45% of total dietary FO without negatively compromising growth performance or nutritional quality of fish, respectively. Moreover, the study increases our knowledge of FO substitution in marine herbivorous fish. | |||
TO cite this article:LI Yuanyou,XU Shude. Effects of replacement of dietary fish oil with soybean oil on growth performance and tissue fatty acid composition in marine herbivorous teleost Siganus canaliculatus[OL].[27 July 2011] http://en.paper.edu.cn/en_releasepaper/content/4436868 |
3. Effect of Seasons on Volatile Compounds of Dorsal Meat in Grass Carp | |||
Wang Xichang,Shi Wenzheng | |||
Aquatic Science 31 December 2010 | |||
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Abstract:In this paper, the volatile compounds of the dorsal meat of cultured grass carp in different seasons were identified by SPME(solid phase micro-extraction)-GC (gas chromatography)-MS (mass spectrometry). The results showed that SPME was effective to analysis of the volatiles in grass carp. According to GC-MS, 21, 43, 42 and 42 volatile compounds were detected in dorsal meat of grass carp in spring, summer, autumn and winter respectively. The volatile components are mostly carbonyl compounds and alcohols, the respective relative contents are above 90%. The relative content of the volatile compounds of cultured grass carp in different seasons has the significance difference by ANOVA. Seasons have greater effect on the volatile compounds of dorsal meat of grass carp. The study will enrich the theoretical knowledge of flavor chemistry and provide an effective solution to the current situation of low proportion of fish processing. | |||
TO cite this article:Wang Xichang,Shi Wenzheng. Effect of Seasons on Volatile Compounds of Dorsal Meat in Grass Carp[OL].[31 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4402985 |
4. Rapid detection of virulent factors of Aeromonas spp. by enzyme-linked Immunosorbent assay on nitrocellulose membranes (Dot-ELISA) | |||
Chu Weihua,Lu Chengping | |||
Aquatic Science 18 October 2005 | |||
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Abstract:With the antiserum for extracellular products (hemolysin and protease) of virulent strain of Aeromonas hydrophila J-1 from rabbits, the Dot-ELISA was developed for the detection of virulent factors of Aeromonas spp.. The sensitivity of the method was high, with a detection limit of 78ng/ml ECPs, All the Aeromonas species detected were positive and no cross-reaction with Edwardsiella tarda, Vibrio anguilarum, Yersinia ruckeri , Edwardsiella tarda, which often infect fishes. Only slightly cross-reaction with Escherichia coli, The agreement between Dot-ELISA and normal detection of ECPs in the standard strains(n=23) and samples (n=120) were 73.9% and 87.2%. The whole reaction can be finished within 24 hours. It can be considered as a useful method for rapid diagnosis of virulent factors of these harmful bacteria. | |||
TO cite this article:Chu Weihua,Lu Chengping. Rapid detection of virulent factors of Aeromonas spp. by enzyme-linked Immunosorbent assay on nitrocellulose membranes (Dot-ELISA)[OL].[18 October 2005] http://en.paper.edu.cn/en_releasepaper/content/3281 |
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