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1. Cloning, phylogeny and expression correlated to gonads development of Leptin genes in blunt snout bream | |||
ZHAO Honghao,ZENG Cong,YI Shaokui,WAN Shiming,Chen Baixiang,GAO Zexia | |||
Aquatic Science 14 November 2015 | |||
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Abstract:To investigate the leptin related genes expression patterns and their possible function during the gonadal development in fish, the cDNA and genomic sequences of leptin, leptin receptor (leptinR), and leptin receptor overlapping transcript like-1 (leprotl1) were cloned and their expression levels were quantified in the different gonadal development stages of Megalobrama amblycephala. The results showed that the full length cDNA sequences of leptin, leptinR, and leprotl1were 953, 3432 and 1676 bp, coding 168, 1,082, and 131 amino acid polypeptides, and the genomic sequences were 1836 bp, 28528 bp and 5480 bp, which respectively had 3, 15 and 4 exons, respectively. The phylogenetic analysis revealed that three genes were relatively conserved in fish species. Quantitative real-time PCR results showed that the three genes were ubiquitously expressed in all examined tissues during the different gonadal development stages. The leptin and leptinR took part in the onset of puberty, especially in female M. amblycephala, by increasing the expression levels in brain during the stage I to III of ovary. The expression levels of leptin and leptinR had significant differences between male and female in HPG axis tissues (P<0.05). The leptinR had the same variation tendency with leptin, but the opposite changes of expression levels were found in leprotl1, which may resist the expression of leptinR for inhibiting the function of leptin in target organ. These findings revealed details about the possible role of these genes in regulating gonadal maturation in fish species. | |||
TO cite this article:ZHAO Honghao,ZENG Cong,YI Shaokui, et al. Cloning, phylogeny and expression correlated to gonads development of Leptin genes in blunt snout bream[OL].[14 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4661884 |
2. Comparative analysis of nuclear oligomerization domain proteins (NOD)-1 and NOD2 in orange-spotted grouper (Epinephelus coioides) | |||
HOU Qinghua,LU Danqi,YI Shibai,DING Xu,SUN Yan,ZHANG Huixian,LIU Xiaochun,ZHANG Yong,LIN Haoran | |||
Aquatic Science 25 December 2012 | |||
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Abstract:Nucleotide-binding oligomerization domain-containing proteins-1 and-2 (NOD1 and NOD2) are members of the NOD-like receptors (NLRs) family. They are both cytoplasmic receptors, and sense microbial infections/danger molecules to induce host innate immune response. In this study, the full-length ORF sequences of NOD1 and NOD2 were cloned, and the putative amino acid sequences were identi?ed in orange-spotted grouper (Epinephelus coioides). The complete open reading frame (ORF) of grouper NOD1 contained 2823 bp encoding a 940 amino acid protein. Grouper NOD2 cDNA contained a 2967 bp ORF, encoding a protein of 988 amino acid residues. Both grouper NOD1 and NOD2 had similar domains to human and fish counterparts. Phylogenetic tree analysis showed that grouper NOD1 clustered with grass carp, zebra?sh and channel cat?sh, while NOD2 was most closely related to fugu. Expression patterns of grouper NOD1 and NOD2 were next studied. NOD1 had the highest level of expression in skin while NOD2 in trunk kidney. Post Vibrio alginolyticus (strain EcGS020401), lipopolysaccharide (LPS) or PolyI:C challenges, gene expression of grouper NOD1 and NOD2 was stimulated to different extents. NOD1 showed a significant enhancement after LPS stimulation, but NOD2 increased more significantly after PolyI:C invasion, indicating that NOD1 and NOD2 may exert different effects on the eradication of bacteria and virus. The adaptor protein RIP-like-interacting CLARP kinase (RICK) and downstream molecule interleukin-8 (IL-8) were also induced at different levels after stimulation, which indicated that NOD1 and NOD2 signal transduction was involved in grouper innate immune protection against bacterial and viral infections. | |||
TO cite this article:HOU Qinghua,LU Danqi,YI Shibai, et al. Comparative analysis of nuclear oligomerization domain proteins (NOD)-1 and NOD2 in orange-spotted grouper (Epinephelus coioides)[OL].[25 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4499074 |
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