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	1. Development of Monoclonal Antibodies Suitable for Detection of Salmonella Enteritidis by Biosensor
	ZHANG Xiaoguang,WANG Zijie,ZHANG Zuomin,WU Pingping,LIU Shijie,ZHOU Hongru
	Food Science and Technology 15 April 2018
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	Abstract:Monoclonal antibodies (MAbs) suitable for detection of Salmonella Enteritidis (SE) by biosensor were prepared and characterized. Indirect enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) biosensor were used for screening of the hybridoma cells secreting MAbs specific to the pathogen. Based on the reactivity of MAbs against the target pathogen by SPR biosensor, MAbs were selected. The clones of 3-5H-3F and 3-7A-8B were found with high reactivity against boiled SE and very low against boiled S. Typhimurium and mixture samples including various non-target bacteria. These two clones were selected for detection of SE and anti-SE MAbs were purified from the culture supernatants. Results showed that MAbs selected via ELISA method, however, may not suitable for detection of target pathogen by using biosensor. The MAbs obtained after screening by the combined method showed a capacity to detect SE by using SPR biosensor as well as ELISA. These MAbs are useful for detection of SE by using biosensor and are expected to contribute to the development of rapid detection of SE in food samples.
	TO cite this article:ZHANG Xiaoguang,WANG Zijie,ZHANG Zuomin, et al. Development of Monoclonal Antibodies Suitable for Detection of Salmonella Enteritidis by Biosensor[OL].[15 April 2018] http://en.paper.edu.cn/en_releasepaper/content/4744606


	2. Genistein Ameliorates Non-alcoholic Fatty Liver Disease by Targeting Thromboxane A2 Pathway
	Wang Wenzhe,Li Haitao,
	Food Science and Technology 09 March 2018
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	Abstract:Non-alcoholic fatty liver disease (NAFLD) is now a public health issue worldwide, but no drug has received approval yet. Here we demonstrated that genistein, an isoflavonoid derived from soybean, effectively attenuated high fat diet-induced NAFLD progression by targeting thromboxane A2 (TXA2) pathway. Analysis of profile of eicosanoid synthesis pathways in liver unexpectedly revealed that TXA2 pathway was aberrantly active in NAFLD. Switching off TXA2 biosynthesis by aspirin effectively suppressed high-fat induced NAFLD progression. In parallel, genistein was identified as a natural thromboxane A2 receptor (TBXA2R) antagonist and successfully applied in NAFLD prevention. Phenotypically, treatment with either aspirin or genistein suppressed body weight gain, attenuated liver steatosis, normalized aminotransferase abnormalities and improved glucose tolerance. Further mechanism study suggested that TXA2 pathway was required for tumor necrosis factor-alpha (TNF-α) induced hepatic insulin resistance. Collectively, our findings established the critical pathophysiological role of the TXA2 pathway in NAFLD, and provided a rationale for introducing a strategy targeting TXA2 for NAFLD prevention and management.
	TO cite this article:Wang Wenzhe,Li Haitao,. Genistein Ameliorates Non-alcoholic Fatty Liver Disease by Targeting Thromboxane A2 Pathway[OL].[ 9 March 2018] http://en.paper.edu.cn/en_releasepaper/content/4743759

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	3. Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition
	Jiayu Gu,Bingyong Mao,Shumao Cui,Jianxin Zhao,Hao Zhang,Wei Chen
	Food Science and Technology 08 March 2018
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	Abstract:Fructooligosaccharides (FOS) are a well-known class of prebiotic and are considered to selectively stimulate the growth of bifidobacteria in the gut. Previous studies focused on the stimulated growth of Bifidobacterium, but did not further investigate the bifidobacterial composition, i.e. the specific species that was were stimulated. In this study mice were fed with FOS in different doses (0%, 5%, and 25%) for 4 weeks and the composition of fecal microbiota, in particular Bifidobacterium, was analyzed by sequencing the V3-V4 region and the groEL gene on the MiSeq platform, respectively. In the high-dose group (25% FOS) the relative abundance of Actinobacteria was significantly increased, which was mainly contributed by Bifidobacterium. At the genus level the relative abundances of Blautia and Coprococcus were also significantly increased. Through the groEL sequencing 14 species of Bifidobacterium were identified, among which Bifidobacterium pseudolongum (B. pseudolongum) was most abundant. After FOS treatment, B. pseudolongum became almost the sole bifidobacterial species (>95%). We isolated the B. pseudolongum strains and, by high performance liquid chromatography (HPLC), demonstrated their ability to metabolize FOS. Therefore, we concluded that FOS significantly stimulated the growth of B. pseudolongum in mice. Further investigations are needed to reveal the mechanism of selectiveness between FOS and B. pseudolongum, which would aid our understanding of the basic principles of dietary carbohydrates and host health.
	TO cite this article:Jiayu Gu,Bingyong Mao,Shumao Cui, et al. Effects of Different Doses of Fructooligosaccharides (FOS) on the Composition of Mice Fecal Microbiota, Especially the Bifidobacterium Composition[OL].[ 8 March 2018] http://en.paper.edu.cn/en_releasepaper/content/4743709


	4. Enhanced production of natural yellow pigments from Monascus by liquid culture: A study of the fermentation conditions and mycelial morphology
	Lv Jun,Zhang Bobo,Xu Gangrong,Cheung ChiKeung Peter
	Food Science and Technology 22 February 2017
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	Abstract:Natural yellow pigments produced by submerged fermentation of Monascus purpureus have potential economic value and application in the food industry. In the present study, the relationships among fermentation conditions (in terms of pH and shaking/agitation speed), mycelial morphology and the production of Monascus yellow pigments were investigated in both shake-flask and scale-up bioreactor experiments. In the shake-flask fermentation, the highest yield of the Monascus yellow pigments was obtained at pH 5.0 and a shaking speed of 180 rpm. Microscopic images revealed that these results were associated with the formation of freely dispersed small mycelial pellets with shorter, thicker and multi-branched hyphae. Further investigation indicated that the hyphal diameter was highly correlated with the biosynthesis of the Monascus yellow pigments. In a scaled-up fermentation experiment, the yield of the yellow pigments (401 U/mL) was obtained in a 200-L bioreactor, which is the highest yield to the best of our knowledge. The present findings can advance our knowledge on the conditions used for enhancing the production of Monascus yellow pigments in submerged fermentation and facilitate large-scale production of these natural pigments.
	TO cite this article:Lv Jun,Zhang Bobo,Xu Gangrong, et al. Enhanced production of natural yellow pigments from Monascus by liquid culture: A study of the fermentation conditions and mycelial morphology[OL].[22 February 2017] http://en.paper.edu.cn/en_releasepaper/content/4719755


	5. Study on detections of free and bound water in sausage based on multispectral imaging technique
	Wang Ju,LIU Changhong,ZHENG Lei
	Food Science and Technology 19 May 2016
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	Abstract:Free and bound water are the two physical states of moisture, which directly influence on the qualities of meat products. The goal of this work was to explore the potential of multispectral imaging in combination with multivariate analysis for the identification of free and bound water in cooked pork sausages (CPS). Spectra and textures of 144 CPS treated by six salt concentrations (0-2.5%) were analyzed using different calibration models to find the most optimal results for predicting free and bound water contents in CPS. Based on a combination of spectral and textural features, partial least squares regression models performed well for predicting free and bound water contents with the correlation coefficient (r) of 0.832 and 0.918, respectively. The prediction equation was also transferred to each pixel in the image for visualizing the spatial distribution of the two water contents in CPS. The results indicated that multispectral imaging technique has the potential as a fast and non-invasive method for identifying the physical state of moisture in meat products.
	TO cite this article:Wang Ju,LIU Changhong,ZHENG Lei. Study on detections of free and bound water in sausage based on multispectral imaging technique[OL].[19 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4690243


	6. Different Roles of Caffeine, Catechins and Tea Proteins in Creaming of Green Tea from Camellia sinensis
	LIN Xiaorong,CHEN Zhongzheng,ZHANG Yuanyuan,LUO Wei,GAO Xiong,LI Bin
	Food Science and Technology 11 May 2016
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	Abstract:Creaming in green tea (Camellia sinensis) was studied by High Performance Liquid Chromatography (HPLC) and Dynamic Light Scattering (DLS). Catechins, caffeine, and proteins were major components of tea cream, which had greater affinity than any other components for precipitation. Their different roles in creaming were investigated in a series of modulated tea and simulated tea. Modulation of caffeine significantly affected the formation of sediments, but this impact was highly related to the amounts of catechins and proteins in original tea. Interactions among catechins, proteins and caffeine were discussed. Tea proteins had priority to associate with catechins over caffeine. They aggregated with catechins spontaneously, forming colloidal particles of 93.3 nm in average hydrodynamic radius (RH). Addition of caffeine led to larger particle size and higher light scattering. In conclusion, nano-aggregates formed by catechins and proteins in green tea provided nucleus for sediments, whereas caffeine acted as an activator to promote creaming.
	TO cite this article:LIN Xiaorong,CHEN Zhongzheng,ZHANG Yuanyuan, et al. Different Roles of Caffeine, Catechins and Tea Proteins in Creaming of Green Tea from Camellia sinensis[OL].[11 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4689350


	7. Identification and characterization of diacylglycerol acyltransferase in oleaginous yeast Rhodosporidium toruloides
	Zhen Wang,Huaiyuan Zhang,Yuanda Song
	Food Science and Technology 18 March 2016
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	Abstract:Diacylglycerol acyltransferase (DGAT), which catalyzes TAG formation from DAG and acyl-CoA, has been considered to play a vital role in TAG accumulation in oleaginous microorganisms. The genome of oleaginous yeast Rhodosporidium toruloides contains two putative DGAT genes, RtDGATa and RtDGATb, which shared little conserved amino acid coding sequence with each other. Phylogeny tree analysis showed that RtDGATa belonged to DGAT1 family and RtDGATb belonged to DGAT2 family. For functional identification of the DGATs, RtDGATa and RtDGATb were individually expressed in Saccharomyces cerevisiae TAG-deficient quadruple mutant (H1246). RtDGATa had obvious preference for monounsaturated fatty acids, however the expression of RtDGATa did not alter the TAG content in S. cerevisiae H1246, and it had non-involvement in TAG accumulation according to its mRNA expression level in R. toruloides. The expression of RtDGATb could completely resume TAG biosynthesis in S. cerevisiae H1246. Substrate preference experiments revealed that RtDGATb preferred unsaturated fatty acids over saturated fatty acids, but not C18:3. Only the expression pattern of RtDGATb was related to the process of fatty acid biosynthesis, suggesting that RtDGATb plays an important role in lipid accumulation in R. toruloides.
	TO cite this article:Zhen Wang,Huaiyuan Zhang,Yuanda Song. Identification and characterization of diacylglycerol acyltransferase in oleaginous yeast Rhodosporidium toruloides[OL].[18 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4681339


	8. Antioxidant activity of marine lactic acid bacteria strains
	GAO Yuan,TUO Yanfeng,QIAN Fang,JIANG Shujuan,MU Guangqing
	Food Science and Technology 18 February 2016
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	Abstract:Six marine lactic acid bacteria (LAB) strains isolated from fish intestines and scallop digestive glands were investigated for their scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. Then their resistance to hydrogen peroxide, scavenging hydroxyl radical, superoxide anion radical activity, reducing power, Superoxide Dismutase (SOD) activity and Glutathione (GSH) content were measured. When Lactobacillus casei Y3 and Lactobacillus plantarum Y44 were testing antioxidant effect of oxidative stress in HT-29 cells, the cell viability increased significantly compared to H2O2 group. Marine LAB Lactobacillus casei Y3 and Lactobacillus plantarum Y44 could be considered as a new type of probiotics resource.
	TO cite this article:GAO Yuan,TUO Yanfeng,QIAN Fang, et al. Antioxidant activity of marine lactic acid bacteria strains[OL].[18 February 2016] http://en.paper.edu.cn/en_releasepaper/content/4678348


	9. Lipase-catalyzed synthesis and the lipid metabolic effect of phytosterol esters
	Pang Min,Zheng Haijie,Jiang Shaotong
	Food Science and Technology 17 November 2015
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	Abstract:In this paper, the esterifications of phytosterol with oleic acid catalyzed by Candida rugosa lipase in organic solvent were studied. The esterification conditions (temperature, substrate molar ratio, reaction time and catalyst amount) were optimized by response surface methodology (RSM). The phytoserol linoleic acid ester was also prepared via lipase-catalyzed. The lipid metabolic effect of equivalent intakes of phytoserol oleic/linoleic acid esters and that of different intakes of the same ester were compared. The results demonstrated that the highest phytoserol oleic acid ester esterification degree of 77.43% was obtained under the optimized conditions: temperature of 41℃, reaction time of 19h, substrate molar ratio (oleic acid: phytosterol) of 2.4:1 and catalyst amount of 7%. The chemical structure of phytoserol oleic acid ester was confirmed by FT-IR and GC-MS. The prepared phytosterol esters decreased significantly TC, LDL-C, LDL-C/HDL-C, ApoAⅠ, ApoAⅠ/ApoB in serum and liver weight, HI, hepatic TC, hepatic TG, while they also increased significantly HDL-C and ApoB in serum. No significant changes were found in the concentrations of TG. In contrast, linoleic acid ester could have a advantage effect on cholesterol-lowering and antioxidant capacity over oleic acid ester.
	TO cite this article:Pang Min,Zheng Haijie,Jiang Shaotong. Lipase-catalyzed synthesis and the lipid metabolic effect of phytosterol esters[OL].[17 November 2015] http://en.paper.edu.cn/en_releasepaper/content/4662571


	10. Isolation, Purification and Identification of Torularhodin from Sporidiobolus pararoseus by Column Chromatography and its Anti-Cancer Effect on LNCaP Cells
	DU Chao,SHI Qiuyu,WU Jialing,CUI Yan,LI Yingchao,WEI Liangxin,QIAN He
	Food Science and Technology 20 October 2015
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	Abstract:In the present study, an isolation and purification method of torularhodin from the fermentation products of Sporidiobolus pararoseus by column chromatography was developed. The purity of the pigment was determined to be above 96% by High Performance Liquid Chromatography (HPLC). The spectroscopic data of torularhodin were obtained by HPLC-Diode Array Detector (DAD), Liquid Chromatography (LC) - Electronic Spray Ion (ESI) - Mass Spectrometry (MS), Fourier - Transform Infrared spectroscopy (FTIR), and Raman spectroscopy. This study was also designed to elucidate the inhibition of torularhodin on the growth of prostate cancer LNCaP cells. Torularhodin significantly suppressed the proliferation of LNCaP cells in a dose-dependent manner. After the treatment with 30 μmol β-carotene, lycopene and torularhodin for 24 h, the growth was decreased by 20.5 ± 3.1%, 27.5 ±3.7%, and 60.2 ±1.8% as showed in WST-1 assay. Therefore, torularhodin deserves further study as a potential method to prevent certain kinds of cancer.
	TO cite this article:DU Chao,SHI Qiuyu,WU Jialing, et al. Isolation, Purification and Identification of Torularhodin from Sporidiobolus pararoseus by Column Chromatography and its Anti-Cancer Effect on LNCaP Cells[OL].[20 October 2015] http://en.paper.edu.cn/en_releasepaper/content/4658372

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