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	1. Tanshinone IIA Improved Non-alcoholic Fatty Liver Disease via A β2-AR-LKB1-AMPK Signaling Axis
	Zheng Xu,Li Haitao
	Food Science and Technology 03 February 2020
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	Abstract:No therapeutic options currently exist for nonalcoholic fatty liver disease (NAFLD). Although commonly prescribed for cardiovascular diseases, Danshen (Salvia miltiorrhiza Bunge) has been used historically in folk medicines against chronic liver diseases, but its potential in NAFLD therapy remains uncertain. Here we reported that Tanshinone IIA (TAN-IIA), a principal constituent of Danshen, effectively ameliorated experimental NAFLD via transactivation of AMP-activated protein kinase. Using a mouse model, we established that tanshinone IIA effectively attenuated high-fat-diet induced obesity, hepatomegaly and liver steatosis. Mechanistically, we found that β2-adrenergic receptor (β2-AR) was down-regulated in liver tissues of obese mice. Tanshinone IIA might function as a β2-AR agonist, increase the intracellular cAMP levels, and turn on liver kinase B1 (LKB1)-AMP-activated protein kinase (AMPK)-acetyl-CoA carboxylase 2 (ACC2) signaling axis to promote fatty acid oxidation. Collectively, tanshinone IIA might merit investigation as a potential therapeutic agent for NAFLD especially in those patients with obesity.
	TO cite this article:Zheng Xu,Li Haitao. Tanshinone IIA Improved Non-alcoholic Fatty Liver Disease via A β2-AR-LKB1-AMPK Signaling Axis[OL].[ 3 February 2020] http://en.paper.edu.cn/en_releasepaper/content/4750630


	2. Comparative genomics and proteolytic enzymatic analysis of lactobacillus helveticus reveals strain biodiversity
	Qi Yanran,Liu Xiaoming
	Food Science and Technology 12 March 2019
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	Abstract:Lactobacillus helveticus is a starter culture in the manufacture of Swiss cheese, Italian aged cheeses, and fermented milk products. Its growth relies on a complex proteolytic system that includes cell-envelope proteinases (CEPs) and peptidases. There were only a few strains of L. helveticus whose proteolytic systems in terms of genotype and phenotype have been characterized. In this study, a phylogenetic configuration and a pan/core genome analysis and an evaluation of the proteolytic enzymatic activities were conducted for 50 trains of L. helveticus including 38 isolated from various ecological niches in our lab. Three L. helveticus strains formed a phylogenetic lineage that was clearly separated from the other strains based on the phylogenetic tree, with the genome sizes from 1.798 to 2.255 Mbp. Diversity in key enzymatic activities was observed among L. helveticus strains. This study provides insight into the genetic and phenotypic diversity of L. helveticus isolated from various environments and the potential application in fermented dairy products.
	TO cite this article:Qi Yanran,Liu Xiaoming. Comparative genomics and proteolytic enzymatic analysis of lactobacillus helveticus reveals strain biodiversity[OL].[12 March 2019] http://en.paper.edu.cn/en_releasepaper/content/4747771


	3. Signals induced by exogenous nitric oxide and their role in controlling brown rot disease in postharvest peach fruit
	ZHANG Chang,LI Guangjin,LEI Zhonghua,LIU Yunyun,REN Jiyun,SHI Jingying
	Food Science and Technology 26 May 2014
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	Abstract:Recent evidence suggests that nitric oxide (NO) signaling plays an important role in plant-pathogen interactions. In this study, the control effect on brown rot disease caused by Monilinia fructicola and the signal role of NO in the elicitation of defense responses in peach fruit were investigated. It was found that 15 μM NO solution induced disease resistance of harvested peaches and as a potentiated elicitor, NO induced the high production rate of endogenous NO and superoxide (O2ˉ), hydrogen oxide (H2O2) content, and the activities of NADPH oxidase and Ca2+-ATPase of peach fruit. Aconitase activity in peach fruit was inhibited by NO. After partial purified, aconitase activity was inhibited by sodium nitroprusside (SNP) and H2O2, however, the inhibition could be relieved by carboxy-2-phenyl-4,4,5,5-tetramethylimidazoline -1-oxyl 3-oxide (cPTIO) or catalase (CAT), indicating that defense response and signals induced by NO transduction depend on aconitase and conditions leading to elevated levels of NO or H2O2 would inactivate aconitase directly in peach fruit. Treatment with NO resulted in accumulation of salicylic acid (SA) during the period of storage. Higher level of jasmonate acid (JA) were detected in NO-treated fruit 48 h after the treatment. But after NO was removed, the level of SA and JA were lower obviously than that of the control. The results suggest that exogenous NO enhances disease resistance of harvested peach fruit to brown rot disease by inducing some signals such as endogenous NO, reactive oxygen species (ROS), SA and JA and inhibiting aconitase activity.
	TO cite this article:ZHANG Chang,LI Guangjin,LEI Zhonghua, et al. Signals induced by exogenous nitric oxide and their role in controlling brown rot disease in postharvest peach fruit[OL].[26 May 2014] http://en.paper.edu.cn/en_releasepaper/content/4598629


	4. Functional characterization of ω9 fatty acid desaturase in oleaginous fungus Mortierella alpina by homologous expression
	Kai Du,Haiqin Chen,Guangfei Hao,Xiaoyun Huang,Danhui Hao,Zhennan Gu,Hao Zhang,Wei Chen,YongQ Chen
	Food Science and Technology 18 March 2014
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	Abstract:In oleaginous fungus Mortierella alpina, Δ9 fatty acid desaturases are the first key enzyme during unsaturated fatty acid biosynthesis. There are three Δ9 fatty acid desaturase genes in Mortierella alpina. In contrast to Δ9-I and Δ9-II, ω9 (Δ9-III) displayed negligible transcriptionally activity under culture conditions. Thus, the intrinsic function and its substrate specificity remain to be determined. In this work, the ω9 fatty acid desaturase from M. alpina ATCC 32222 was cloned and homologously over-expressed. Lipid analysis revealed that ω9 fatty acid desaturase had moderate activities using palmitic acid (C16:0) and stearic acid (C18:0) as substrates. Palmitoleic acid (C16:1) and oleic acid (C18:1) contents increased 3.25- and 1.95-fold in ω9 over-expressing clones compared to the wild-type strain. Low temperature (12 C) significantly improved the conversion rate of C16:0 to C16:1 (9.24-fold increase) by ω9 desaturation. Moreover, the ω9 fatty acid desaturase may have specific activities with very long-chain saturated fatty acid substrates since a small amount of C24:1 was detected in ω9 gene over-expressing strains but none in the wild-type strain.
	TO cite this article:Kai Du,Haiqin Chen,Guangfei Hao, et al. Functional characterization of ω9 fatty acid desaturase in oleaginous fungus Mortierella alpina by homologous expression[OL].[18 March 2014] http://en.paper.edu.cn/en_releasepaper/content/4590290


	5. Aggregation and adhesion properties of 22 Lactobacillus strains
	Hanli Yu,Yanfeng Tuo,Lianzhong Ai,Zhengjun Wu,Wei Chen,Benhen Guo
	Food Science and Technology 24 December 2012
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	Abstract:In this paper, the auto-aggregating, co-aggregating, hydrophobicity and adhering abilities of 22 Lactobacillus strains belonging to different species were assessed. There was no correlation between auto-aggregation and adhesion of the strains belonging to different species, while there was positive correlation (P<0.05) between auto-aggregation and adhesion of the strains belonging to same species. After treating by guanidine HCl, the auto-aggregating and adhering abilities of some Lactobacillus strains decreased, indicating that surface-bound protein and other macro-molecular played role in the adhering and auto-aggregating abilities. The strains Lactobacillus plantarum 20 and 66 had higher adhdsion and coaggregation abilities should be further studied for their probably probiotic properties. Aggregating, co-aggregating and adhering abilities of Lactobacillus strains could be just used as the preliminary criteria for selecting strains having probiotic potential.
	TO cite this article:Hanli Yu,Yanfeng Tuo,Lianzhong Ai, et al. Aggregation and adhesion properties of 22 Lactobacillus strains[J].


	6. Screening of lactobacilli with antagonistic activity against enteroinvasive Escherichia coli
	Wenyu Liu,Xiaoming Liu,Fengwei Tian,Qiuxiang Zhang,Gang Wang,Hao Zhang,Wei Chen
	Food Science and Technology 15 March 2012
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	Abstract:In the present study, 339 lactic acid bacteria (LAB) strains, isolated from fermented dough, pickles, salted meat, baby feces and fermented dairy products, were screened for the antagonistic activity against enteroinvasive Escherichia coli (EIEC). Through the analysis of the inhibition zone of the lactobacilli spent culture supernatant (SCS) on EIEC growth, 22 LAB strains were selected. Using the in vitro assays of adhesion to HT-29 cell lines and tolerance to gastrointestinal conditions (acid and bile), two isolates, Lactobacillus plantarum CCFM 233 and Lactobacillus plantarum CCFM 231, were shown not only to inhibit the growth of EIEC significantly, but also to have high adhesion to HT-29 cell lines and good tolerance to low acid and high bile. Furthermore, the two strains could strongly antagonize the adhesion and invasion of EIEC to the HT-29 cell lines. This study suggests that Lactobacillus plantarum CCFM 233 and Lactobacillus plantarum CCFM 231 could be used as potential probiotics against EIEC.
	TO cite this article:Wenyu Liu,Xiaoming Liu,Fengwei Tian, et al. Screening of lactobacilli with antagonistic activity against enteroinvasive Escherichia coli[OL].[15 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4471789


	7. Heat and Mass Transfer Mathematic Model of Cabbage Vacuum Cooling and Experimental Research
	Kan Ankang,Han Houde,Geng Jinliang
	Food Science and Technology 09 April 2010
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	Abstract:The cabbage was taken as the study object in this paper. The heat and mass transfer mathematical models for the cylindrical shape vegetable were built and the experimental method was introduced to prove them. The comparison and analysis, under the certain vacuum degree of the vacuum chamber, was done between the simulation and the experiment. And the differences of the vacuum degree in the chamber, the temperature change of the cabbage, the relative humidity in the chamber and the cabbage mass loss, in the experiment and the simulation, were all recorded. The reason of the parameters variance and existent deference was also analyzed in this paper. The mathematical models and the result was useful for the study on the cylindrical vegetable vacuum pre-cooling technology.
	TO cite this article:Kan Ankang,Han Houde,Geng Jinliang. Heat and Mass Transfer Mathematic Model of Cabbage Vacuum Cooling and Experimental Research[J].

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