Abstract:5-Fluorouracil (5-FU) treatment in Caenorhabditis elegans(C.elegans) leads to slow developmental rate, reduces brood size, small body size, and truncates lifespan. Using Digital Gene Expression (DGE) profiling analysis, the potential pathways and genes that could be involved in declining the embryos of C. elegans have identified. Here, when C.elegans treated by 5-FU, three down-regulated genes were identified, including col-131, col-150 and dpy-5, which belong to ECM-receptor interaction pathway and Focal adhesion and one up-regulated gene col-121 which acts in Focal adhesion. Furtherly, RNAi-mediated gene knock down method was used to examine their RNAi phenotypes. The result showed that dpy-5(RNAi) worms were short-body, dumpy, and displaying a serious defect in fertility. col-121(RNAi) worms were slender, long, and slightly altered in body development. In addition, col-121(RNAi) worms displayed abnormal body morphology. Furthermore, knocking down of col-131 has subtle side effect in C.elegans fertility; however, col-131 (RNAi) worms could rescue 5-Fu toxic effect on C.elegans on fertility and lifespan defect. Taken together, the results revealed the essential role for col-150, dpy-5 and col-121 in C.elegans fertility decline. Our data also suggested that col-131 may be an essential gene in ECM-receptor interaction pathway and Focal adhesion and regulate other cuticle collagen genes including col-150, dpy-5, and col-121.

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	1. The effects of collagen genes col-131, col-150, dpy-5 and col-121 on fertility and development of Caenorhabditis elegans
	Lu Zhaolian,Fu Ximei,Zhang Yan,Liang Hongmei,Liu Xin,Chen Gen
	Basic Medicine 10 October 2015
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	Abstract:5-Fluorouracil (5-FU) treatment in Caenorhabditis elegans(C.elegans) leads to slow developmental rate, reduces brood size, small body size, and truncates lifespan. Using Digital Gene Expression (DGE) profiling analysis, the potential pathways and genes that could be involved in declining the embryos of C. elegans have identified. Here, when C.elegans treated by 5-FU, three down-regulated genes were identified, including col-131, col-150 and dpy-5, which belong to ECM-receptor interaction pathway and Focal adhesion and one up-regulated gene col-121 which acts in Focal adhesion. Furtherly, RNAi-mediated gene knock down method was used to examine their RNAi phenotypes. The result showed that dpy-5(RNAi) worms were short-body, dumpy, and displaying a serious defect in fertility. col-121(RNAi) worms were slender, long, and slightly altered in body development. In addition, col-121(RNAi) worms displayed abnormal body morphology. Furthermore, knocking down of col-131 has subtle side effect in C.elegans fertility; however, col-131 (RNAi) worms could rescue 5-Fu toxic effect on C.elegans on fertility and lifespan defect. Taken together, the results revealed the essential role for col-150, dpy-5 and col-121 in C.elegans fertility decline. Our data also suggested that col-131 may be an essential gene in ECM-receptor interaction pathway and Focal adhesion and regulate other cuticle collagen genes including col-150, dpy-5, and col-121.
	TO cite this article:Lu Zhaolian,Fu Ximei,Zhang Yan, et al. The effects of collagen genes col-131, col-150, dpy-5 and col-121 on fertility and development of Caenorhabditis elegans[OL].[10 October 2015] http://en.paper.edu.cn/en_releasepaper/content/4657315


	2. Xylocarpin H, a component of Xylocarpus granatum, produces antidepressant-like activities in mice
	YIN Xi,LI Xin,HAO Yaoguang,ZHAO Yiwen,ZHOU Jinghong,SHI Haishui
	Basic Medicine 26 November 2014
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	Abstract:Major depression is a common psychiatric disorder worldwide that imposes a substantial health burden on society. Currently available antidepressants do not meet the clinical needs. Here, we report that Xylocarpin H, an active component of Xylocarpus granatum, has antidepressant-like effects in mouse models of depression. In mouse forced swimming- and tail suspension tests, two validated models of depression, oral administration of Xylocarpin H resulted in a dose-dependent decreased immobility duration within the dose range of 15-50 mg/kg. In addition, Xylocarpin H dose-dependently increased the time in the central zone at doses of 5-50 mg/kg in the locomotion activity test. The antidepressant-like activities of Xylocarpin H were associated with its inhibitory effects on hypothalamic-pituitary-adrenal (HPA) axis' hyperactivity in response to acute stress. Interestingly, these effective doses of Xylocarpin H did not affect locomotor- and HPA axis activities in the absence of stress. In summary, the present study, for the first time, demonstrated that Xylocarpin H exerts antidepressant-like effects in mouse behavioral models of depression, likely by inhibiting HPA axis systems. These data provide a strong basis for developing Xylocarpin H as a novel antidepressant agent for the treatment of major depression disorders.
	TO cite this article:YIN Xi,LI Xin,HAO Yaoguang, et al. Xylocarpin H, a component of Xylocarpus granatum, produces antidepressant-like activities in mice[OL].[26 November 2014] http://en.paper.edu.cn/en_releasepaper/content/4620567

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	3. Association Between Genetic Variants in pre-miRNA and Colorectal Cancer Risk in a Chinese Population
	Lv Meili,Dong Wei,Wei Yonggang,Li Lijuan,Zhang Lushun,Shu Xiaowei,Wang Li,Gao Linbo,Zhang Lin
	Basic Medicine 28 January 2013
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	Abstract:Background: Single nucleotide polymorphisms (SNPs) in pre-miRNAs may alter microRNA expression levels or processing and then contribute to the susceptibility of cancer development. We hypothesized that SNPs in pre-miRNAs may be association associated with the risk of colorectal cancer (CRC). Methods: We used genotyped four common polymorphisms (i.e., rs11614913, rs3746444, rs2910164, and rs2292832) in pre-miRNAs of 353 CRC patients and 540 healthy controls to investigate the association between the SNPs and the risk of CRC using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) genotyped four common polymorphisms(rs11614913, rs3746444, rs2292832 and rs2910164) in pre-miRNAs of 353 CRC patients and 540 healthy controls to investigate the association between them and the risk of CRC assay..Results: The rs11614913 CT, TT genotypes and T allele were associated with an increased risk of CRC compared with the CC genotype and C allele (CT vs. CC: OR＝7.34, 95% CI, 3.76-14.34; TT vs. CC: OR＝13.66, 95% CI, 6.76-27.6; T vs. C: OR＝1.99, 95% CI, 1.63-2.42, respectively). Interestingly, using the rs2910164 GG genotype as a reference, the rs2910164 GC genotype was associated with an increased risk of CRC (OR＝1.49, 95% CI, 1.02-2.18), whereas the rs2910164 CC genotype was associated with a decreased risk of CRC (OR＝0.58, 95% CI, 0.37-0.93). When compared with the rs2910164G allele, rs2910164 C allele was associated with a reduced risk of CRC (OR＝0.80, 95% CI, 0.66-0.97, P=0.02). significantly increased CRC risk were found to be associated with CT genotype (OR＝0.136, 95% CI: 0.070-0.266) and TT genotype (OR＝0.073, 95% CI: 0.036-0.148) vs. CC genotype of rs11614913, CT (OR＝0.166, 95% CI: 0.037-0.735,) and TT genotype (OR＝0.167, 95% CI: 0.038-0.729) vs. CC genotype of rs3746444, and GC genotype (OR＝0.670, 95% CI: 0.460-0.977) vs. GG genotype of rs2910164. Unfortunately, there were no statistically significant differences between cases and controls in genotype of rs2292832. When compared with the alleles, significantly increased CRC risk were found to be associated with T allele (OR＝0. 530, 95% CI: 0.413-0.613) vs. C allele in rs11614913. There were no statistically significant differences between cases and controls in alleles of rs3746444, rs2292832 and rs2910164.Conculsion: These findings suggest that rs11614913 and rs2910164 polymorphisms may be associated with the etiology of CRC.
	TO cite this article:Lv Meili,Dong Wei,Wei Yonggang, et al. Association Between Genetic Variants in pre-miRNA and Colorectal Cancer Risk in a Chinese Population[OL].[28 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4517990


	4. microRNA-133b regulates MMP9 and inhibits migration and invasion in human colorectal carcinoma cells
	Hu Gui,Chen Daojin,Li Xiaorong
	Basic Medicine 19 December 2012
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	Abstract:Objective: To explore the effect and mechanism of microRNA-133b in the regulation of colorectal cancer cell migration and invasion. Methods: To construct eukaryotic expression vector of microRNA-133b and stable tansfection into human colorectal cancer cell SW-620; wound-healing assay and Matrigel invasion assay was performed to determine the effect of microRNA-133b on in vitro SW-620 migration and invasion; The possible target of miR-133b were predicted by a web-based computer program and verified by Dual-luciferase assay system. Results: The expression of miR-133b was dramatically greater in SW-620-133b compared with SW-620 (P＜0.05); In sw-620 cells, expression of microRNA-133b significantly inhibited tumor cell migration and invasion(P＜0.05); MMP9 was the candidate target of microRNA-133b and verified by Dual-luciferase assay. Conclusions: microRNA-133b suppresses tumor cell migration and invasion through modulation of the MMP9 signaling pathway.
	TO cite this article:Hu Gui,Chen Daojin,Li Xiaorong. microRNA-133b regulates MMP9 and inhibits migration and invasion in human colorectal carcinoma cells[OL].[19 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4506084


	5. Transplantation of parthenogenetic embryonic stem cells ameliorates cardiac dysfunction and remodeling after myocardial infarction
	Liu Yi,Ye Xiaoying,Mao Lina,Cheng Zhaokang,Yao Xinpeng,Jia Xiaohua,Mao Duo,Ou Lailiang,Li Zongjin,Che Yongzhe,Liu Na,Liu Lin,Kong Deling
	Basic Medicine 14 December 2012
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	Abstract:Aims: Parthenogenetic embryonic stem cells (pESCs) are derived from artificially activated oocytes without fertilization and therefore raise minimal ethical concerns and may serve as attractive candidates for regenerative medicine. We thought to investigate whether pESCs can repair myocardial infarction (MI). Methods and Results: Mice (n=89) survived coronary ligation randomly received undifferentiated pESCs, ESCs, or saline. Sham-operated mice received no treatment (n=21). After 7 days, pESCs transplantation promoted pro-angiogenic factors secretion and reduced infiltrated leukocytes. pESCs-treated hearts, superior to ESC group, showed prevented cardiac remodeling and enhanced angiogenesis in and 30 days post MI. Heart contractile function was notably improved by administration of pESCs by 30 days. Moreover, tissues regenerated from the engrafted pESCs in the infarcted myocardium were positive for cardiomyocyte, endothelial cell and smooth muscle cell markers. Furthermore, teratoma fomation appeared in ESCs-treated mice in high proportion (6/34), but surprisingly not found in pESCs-treated mice (0/30) by 30 days. Conclusions: Cardiac dysfunction and adverse ventricular remodeling post MI were attenuated by pESCs transplantation, which may represent an effective and relatively safer strategy for autologous cell therapy in females.
	TO cite this article:Liu Yi,Ye Xiaoying,Mao Lina, et al. Transplantation of parthenogenetic embryonic stem cells ameliorates cardiac dysfunction and remodeling after myocardial infarction[OL].[14 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4504322


	6. Pretreatment of ginsenoside-Rd reduces ischemia-reperfusion injury in isolated rat hearts by increasing coronary flow
	SONG Chun,WANG Liping,WANG Qilong,GAO Mingtang
	Basic Medicine 06 January 2012
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	Abstract:Objective: To evaluate the myocardial effects of ginsenoside-Rd in isolated rat hearts and to investigate the potential protective mechanism of ischemia-reperfusion injury. Methods: All rat hearts were isolated and perfused with modified Krebs-Heinseleit buffer (KHB) using the Langendorff preparation. For ischemia-reperfusion trial groups, hearts were treated with ginsenoside-Rd for 10 minutes after 20 minutes’ equilibrium, followed by 30 minutes’ global ischemia and 120 minutes’ reperfusion. For working heart trial groups, hearts were perfused with KHB containing ginsenoside-Rd for 40 minutes after 20 minutes’ equilibrium. Both of these two randomized controlled trials were performed using verapamil as positive control. Coronary flow (CF), heart rate (HR), left ventricular end diastolic pressure (LVEDP), left ventricular systolic pressure (LVSP), left ventricular developed pressure (LVDP), and rate pressure product (RPP) were collected to analyze the myocardial effects of ginsenoside-Rd. Results: Ginsenoside-Rd significantly increased the CF in both I/R trial and working heart trial (P<0.05). In I/R trial, it also helped lower the LVEDP and increase the RPP (P<0.05). However, in working heart trial, it showed no significant difference on the HR and LVDP (P>0.05).Conclusion: Pretreatment of ginsenoside-Rd of a middle dose could play a protective role in isolated rat ischemia-reperfusion hearts, by increasing the coronary flow rather than influencing cardiac functional properties previously.
	TO cite this article:SONG Chun,WANG Liping,WANG Qilong, et al. Pretreatment of ginsenoside-Rd reduces ischemia-reperfusion injury in isolated rat hearts by increasing coronary flow[OL].[ 6 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4458840


	7. Rapid biomimetic mineralization of chitosan sponges with a facile method in ethanol/water mixed solution
	Li Lihua ,Zhou Changren
	Basic Medicine 18 May 2010
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	Abstract:Mineralization of biopolymers is a hot topic and a promising method for tissue engineering. In this study, biomimetic mineralization of chitosan sponges was performed in a large quantity with a rapid and facile approach. The calcium phosphate apatite was co-precipitated as the precursor for the formation of hydroxyapatite in mixed water-ethanol solution. The component and morphology of the apatite and the nanocomposite hybrid sponges were measured with XRD, TEM, FTIR and ESEM. The mechanism of nucleation and growth of crystals were discussed as well. Results revealed that chitosan matrix improved the crystalline quality of hydroxyapatite (HAP) crystals. With elongation of mineralization time, the mineral layers on the outer surface and inner section of chitosan sponges increased. The chitosan sponge or the microenvironment around it promoted the crystallites quality of HAP phase. Furthermore, the compressive strength and modulus of the HAP-chitosan bio-composites increased to 0.55 MPa and 29.29± 1.25 MPa respectively. Such one-pot approach might be extended to the mineralization of other biopolymers and will have a very broad application in the future.
	TO cite this article:Li Lihua ,Zhou Changren . Rapid biomimetic mineralization of chitosan sponges with a facile method in ethanol/water mixed solution[OL].[18 May 2010] http://en.paper.edu.cn/en_releasepaper/content/4372783


	8. The role of transcription factors Sp1 and YY1 in proximal promoter region in initiation of transcription of the mu opioid receptor gene in human lymphocytes
	Hui Li,Gang Li
	Basic Medicine 30 December 2008
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	Abstract:Although previous studies have shown that the mechanism of the lymphocyte mu opioid receptor (MOR) gene expression was distinctly different from that in the central nervous system, and is involved in several disparate aspects of the immune response, its precise molecular mechanism is still undefined. In this study, we analyzed the proximal promoter region of the MOR gene in lymphocytes to identify the influences of potential trans-acting factors in activating the initiation of the expression of the MOR gene in lymphocytes. The electrophoretic mobility shift assay showed that two transcription factors, Sp1 and YY1, were able to bind the promoter region. Using sequence overlapping probes and mutation assays, we determined that the CCC sequence of Sp1 and the GGC sequence of YY1 binding elements were core sequences, and replacement of these sequences lead to substantial loss of promoter activity. Stimulation with morphine was capable of up-regulating the intracellular level of Sp1 and YY1 proteins. Chromatin immunoprecipitation assays showed that the blockage of naloxone is achieved through down-regulation of transcription factor YY1. Furthermore, coimmunoprecipitation and transfection assays confirmed that the functional interaction of Sp1 and YY1 transcription factors was a crucial step in the initiation of expression of the MOR in lymphocytes. Thus, we conclude that the cooperative interaction of Sp1 and YY1 transcription factors is the critical event triggering the initiation of transcription of the MOR gene in lymphocytes, and this finding will be helpful to understand the pharmacological effect of morphine on lymphocytes.
	TO cite this article:Hui Li,Gang Li. The role of transcription factors Sp1 and YY1 in proximal promoter region in initiation of transcription of the mu opioid receptor gene in human lymphocytes[OL].[30 December 2008] http://en.paper.edu.cn/en_releasepaper/content/27120

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