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There are 29 papers published in subject: > since this site started. |
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1. The mechanisms underlying that injury of skin afferents does not produce neuropathic pain: the role of BDNF | |||
Liu Xian-guo,Zhou Li-Jun | |||
Basic Medicine 18 January 2010 | |||
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Abstract:Although a large body of evidence has shown that peripheral nerve injury usually induces neuropathic pain, there are also clinical studies demonstrating that injury of the sural nerve, which almost only innervates skin, fails to do so. The underlying mechanism, however, is largely unknown. In the present work, we found that the transection of either the gastrocnemius-soleus (GS) nerve innervating skeletal muscle or tibial nerve supplying both muscle and skin, but not of the sural nerve produced a lasting mechanical allodynia and thermal hyperalgesia in adult rats. High-frequency stimulation (HFS) or injury of either the tibial nerve or the GS nerve induced late-phase long-term potentiation (L-LTP) of C-fiber-evoked field potentials in spinal dorsal horn, while HFS or injury of the sural nerve only induced early-phase LTP (E-LTP). Furthermore, HFS of the tibial nerve induced L-LTP of C-fiber responses evoked by the stimulation of the sural nerve and the heterotopic L-LTP was completely prevented by spinal application of TrkB-Fc (a BDNF scavenger). Spinal application of low dose BDNF (10pg/ml) enabled HFS of the sural nerve to produce homotopic L-LTP. Finally, we found that injury of the GS nerve but not that of the sural nerve up-regulated BDNF in DRG neurons, and that the up-regulation of BDNF occurred not only in injured neurons but also in many uninjured ones. Therefore, the sural nerve injury failing to produce neuropathic pain may be due to the nerve containing insufficient BDNF under both physiological and pathological conditions. | |||
TO cite this article:Liu Xian-guo,Zhou Li-Jun. The mechanisms underlying that injury of skin afferents does not produce neuropathic pain: the role of BDNF[OL].[18 January 2010] http://en.paper.edu.cn/en_releasepaper/content/39074 |
2. The direction of synaptic plasticity mediated by C-fibers in spinal dorsal horn is decided by Src-family kinases in microglia | |||
Liu Xian-guo,Zhong Yi | |||
Basic Medicine 15 January 2010 | |||
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Abstract:Previous studies have shown that Src-family kinases (SFKs) are selectively activated in spinal microglia following peripheral nerve injury and the activated SFKs play a key role for the development of neuropathic pain. To investigate the underlying mechanism, in the present study the effect of SFKs on long-term potentiation (LTP) at C-fiber synapses in spinal dorsal horn, which is believed as central mechanism of neuropathic pain, was investigated in adult rats. Electrophysiological data revealed that pretreatment with either microglia inhibitor (minocycline, 200 μM) or SFKs inhibitors (PP2, 100 μM and SU6656, 200 μM) reversed the effect of high frequency stimulation (HFS), that is, HFS, which induces long-term potentiation (LTP) normally, induced long-term depression (LTD) after inhibition of either microglia or SFKs. Western blotting analysis showed that the level of phosphorylated SFKs (p-SFKs) in ipsilateral spinal dorsal horn was transiently increased after LTP induced by HFS, starting at 15 min and returning to control level at 60 min after HFS. Double –labeled immunofluorescence staining demonstrated that p-SFKs were highly restricted to microglia. Furthermore, we found that the inhibitory effects of minocycline or SU6656 on spinal LTP were reversed by spinal application of rat recombinant tumor necrosis factor-α (TNF-α 0.5 ng/ml, 200 μl). HFS failed to induce LTP of C-fiber evoked field potentials in tumor necrosis factor-α receptor-1 knock out (TNFR1 -/- ) mice and in rats pretreated with TNF-α neutralization antibody (0.6 μg/ml, 200μl). The results suggested that in spinal dorsal horn activation of SFKs in microglia might control the direction of plastic changes at C-fiber synapses and TNF-α might be involved in the process. | |||
TO cite this article:Liu Xian-guo,Zhong Yi. The direction of synaptic plasticity mediated by C-fibers in spinal dorsal horn is decided by Src-family kinases in microglia[OL].[15 January 2010] http://en.paper.edu.cn/en_releasepaper/content/39023 |
3. Dynamic urinary proteomic analysis | |||
Sun Wei ,Chen Yong ,Gao Youhe | |||
Basic Medicine 24 March 2009 | |||
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Abstract:Human urinary proteome analysis is a convenient and efficient approach for understanding disease processes affecting the kidney and urogenital tract. Many potential biomarkers have been identified in previous differential analyses; however, dynamic variations of the urinary proteome have not been intensively studied, and it is difficult to conclude that potential biomarkers are genuinely associated with disease rather then simply being physiological proteome variations. In this paper, pooled and individual urine samples were used to analyze dynamic variations in the urinary proteome. Five types of pooled samples (first morning void, second morning void, excessive water-drinking void, random void, and 24 h void) collected in 1 day from six volunteers were used to analyze intra-day variations. Six pairs of first morning voids collected a week apart were used to study inter-day, inter-individual, and inter-gender variations. The intra-day, inter-day, inter-individual, and inter-gender variation analyses showed that many proteins were constantly present with relatively stable abundances, and some of these had earlier been reported as potential disease biomarkers. In terms of sensitivity, the main components of the five intra-day urinary proteomes were similar. The advantages and disadvantages of pooling samples are also discussed. | |||
TO cite this article:Sun Wei ,Chen Yong ,Gao Youhe . Dynamic urinary proteomic analysis [OL].[24 March 2009] http://en.paper.edu.cn/en_releasepaper/content/30668 |
4. Comparative Study of Serum Proteins Between Guizhou Miniature Pig And Human | |||
Younan Chen,Shengfang Qin,Jingqiu Cheng | |||
Basic Medicine 15 January 2009 | |||
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Abstract:Porcine liver synthesized proteins performing efficient physiological function in human body is an essential premise for successful liver xenotransplantation. So, we did preliminary study on porcine serum protein and compared with that of human to gain insight into the functional compatibility in xenotransplantation. Venous blood was collected from 30 Guizhou Miniature Pigs (Sus scrofa) and 30 human volunteers. Total Protein (TP) was detected by biuret and Albumin (Alb) was detected by bromocresol green. Serum proteins were electrophoresed in REP electrophoresis system and the percentage of each subtype was calculated. The concentration of porcine Alb was apparently lower than that of human (p<0.05), while the TP level was similar. Consistently, porcine Alb% was lower than that of human while the concentration of porcine Globulin (Glb) and percentage of each subtype (Alpha1, Alpha2, Beta and Gamma Globulin) are higher than that of human (p<0.05). The results suggested that there were definite differences in contents of serum proteins between human and pig, which would lead to potential functional incompatibility after porcine liver was transplanted into human body. | |||
TO cite this article:Younan Chen,Shengfang Qin,Jingqiu Cheng. Comparative Study of Serum Proteins Between Guizhou Miniature Pig And Human[OL].[15 January 2009] http://en.paper.edu.cn/en_releasepaper/content/27906 |
5. The distribution of human papillomavirus genotypes in uterine cervical lesions in Yanbian of Northern China | |||
Zhao Yiwei ,Lin Hai ,Wu Qunying ,Han Songying ,Zhang Meihua,Lin Zhenhua | |||
Basic Medicine 09 October 2007 | |||
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Abstract:Purposes: To investigate the distribution of human papillomavirus (HPV) genotypes in uterine cervical lesions in Yanbian of Northern China. Materials and Methods: Paraffin embedded blocks of 62 cases of squamous cell carcinomas (SCC), 28 cases of adenocarcinomas, and 143 cases of CIN (CIN-1: 45; CIN-2: 46; CIN-3: 52) were selected from Dept. of Pathology, Yanbian University Hospital and Yanbian Women’s Hospital in the period of 2000-2005. DNA was extracted by using High Pure PCR Template Preparation Kit from all above blocks. And HPV genotypes were detected by using oligonucleotide microarray (HPV DNA-chip). Results: All the 20 cases of normal cervical epithelia are negative for HPV by both TaKaRa PCR and DNA-chip methods. The positive rate of high-risk HPV is 28.9% in CIN-1, 41.3% in CIN-2, 44.2% in CIN-3, 85.5% in SCC, and 82.1% in adenocarcinoma by TaKaRa PCR method. Similarly, it is 26.7% in CIN-1, 41.3% in CIN-2, 40.4% in CIN-3, 82.3% in SCC, and 78.6% in adenocarcinoma by HPV DNA-chip. HPV 16 is the major type in all CIN and SCC cervical lesions. However, in cervical adenocarcinoma, HPV 18 is the most common type (59.1% in HPV positive cases), and HPV 16 is the second type, but still show a high percentage (31.8% in HPV positive cases). There is small number of multi-types HPV detected in CIN-3, SCC, and adenocarcinoma, but none in CIN-1/2. Meanwhile, there is no significant difference on the HPV screening between TaKaRa PCR and HPV DNA-chip methods (p<0.05). Conclusions: HPV 16 is the type most frequently involved in the development of SCC of the cervix, whereas both HPV 18 and 16 play a prominent role in the development of adenocarcinoma of the cervix in Yanbian of Northern China. Both TaKaRa PCR and HPV DNA-chip methods are sensitive for HPV detection, and can be used for the screening of HPV infection status and genotyping in uterine cervical lesions, and maybe helpful for the prediction of the development and progress of CIN-2/3. | |||
TO cite this article:Zhao Yiwei ,Lin Hai ,Wu Qunying , et al. The distribution of human papillomavirus genotypes in uterine cervical lesions in Yanbian of Northern China[OL].[ 9 October 2007] http://en.paper.edu.cn/en_releasepaper/content/15558 |
6. Real Time Detection of 1A-ARs Movement Stimulated by Phenylephrine in Single Living Cell | |||
Xu Ning ,Liang Zhangyi ,Xu Ming ,Guan YingHua ,He Qihua ,Han Qide ,Zhao Xinsheng ,Zhang Youyi | |||
Basic Medicine 17 November 2006 | |||
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Abstract:AIM: To investigate the movement of 1A- adrenergic receptors( 1A-ARs) stimulated by agonist phenylephrine (PE) and the dynamics of receptors movement in real time in single living cells with millisecond resolution. METHODS: We labeled 1A-ARs using monoclonal anti-FLAG antibody and Cy3-conjugated Goat Anti-Mouse IgG and recorded the trajectory of their transport process in the living HEK293A cells stimulated by agonist phenylephrine (PE), then analysis the dynamic properties of them. RESULTS: The specific detection of 1A-ARs on the surface of living HEK293A- 1A cells was achieved. 1A-ARs internalize under stimulation of PE After the cells were stimulated with PE for 20 minutes, apparent co-localization was found between 1A-ARs and F-actins. And after 40 min stimulation of PE, trajectories of approximate linear motion in HEK293A- 1A cells were recorded, and the velocity of them were calculated. CONCLUSION: The specific labeling method on living cell surface provides a convenient means on real-time detection of the behavior of surface receptors. By this method we were able to specifically detect 1A-ARs, record the behavior of individual particles of receptors with 50ms exposure time in real time in single living cell. | |||
TO cite this article:Xu Ning ,Liang Zhangyi ,Xu Ming , et al. Real Time Detection of 1A-ARs Movement Stimulated by Phenylephrine in Single Living Cell[OL].[17 November 2006] http://en.paper.edu.cn/en_releasepaper/content/9683 |
7. Internalization and distribution of three α1-adrenoceptor subtypes in HEK293A cells before and after agonist stimulation | |||
Wang Shuyi,Song Yao,Xu Ming,Han Qide,Zhang Youyi | |||
Basic Medicine 06 November 2006 | |||
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Abstract:AIM: To examine the subcellular distribution of three α1-adrenoceptor (α1-AR) subtypes and their internalization and trafficking upon agonist stimulation in human embryonic kidney (HEK) 293 cells. METHODS: Confocal real-time imaging, ELISA and whole cell 3H-prazosin binding assay were applied to detect the distribution and localization of three α1-AR subtypes. RESULTS: α1A-AR was found both on the cell surface and in the cytoplasm; α1B-AR, however, was predominantly detected on the cell surface while α1D-AR was detected mainly in the intracellular compartments. After stimulation with phenylephrine, localization changes were detected by confocal microscopy for α1A- and α1B-ARs but localization of α1D-ARs was unaffected. Phenylephrine stimulation promoted a more rapid internalization of α1B-ARs than α1A-ARs. We were able to show α1D-AR internalization only when assayed by enzyme-linked immunosorbent assay (ELISA). Whole cell 3H-prazosin binding assay showed that α1A-AR functional receptors were detected both on the cell surface and in the cytoplasm; α1B-ARs, however, were detected predominantly on the cell surface while α1D-ARs were detected mainly intracellularly. Phenylephrine stimulation promoted internalization of α1A- and α1B-ARs. CONCLUSION: These results suggest that phenylephrine stimulation could induce changes in the localization of the three α1-ARs. | |||
TO cite this article:Wang Shuyi,Song Yao,Xu Ming, et al. Internalization and distribution of three α1-adrenoceptor subtypes in HEK293A cells before and after agonist stimulation[OL].[ 6 November 2006] http://en.paper.edu.cn/en_releasepaper/content/9309 |
8. Data analysis of HLA-A*0201 bound peptide nonamers in PDB | |||
Zhang Ruxiang,Huang Qinghai,Zhang Jianqiong,Xie Wei | |||
Basic Medicine 18 May 2006 | |||
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Abstract:HLA structural data were downloaded from PDB. All data were filtered by programming and were managed in the Visual FoxPro (VFP). With Biopolymer module of InsigtII software, hydrogen atoms were added to filtered molecules. Then, the program was utilized to analyze distances of every two Cα atoms of peptide nonamers and amino acid residues of HLA involved within a different distance around 26 peptide nonamers respectively. There were 26 HLA-A*0201 complexes with peptide nonamers in PDB. The position 2(P2) of 21 peptides was Leu. Meanwhile, P9 was either Leu or Val mostly (25/26). The mean number of H-bond between P1 and molecule HLA-A*0201 was 1.86, while those at P2 and P9 were 1.81, 1.95 respectively. By method of moment, some distances of two Cα atoms appeared normal school. Some amino acid residues of HLA, such as Tyr7, Glu63, Lys66, His70, Thr73, Ser77, Leu81, Tyr99, Thr143, Trp147 and Tyr171, appeared above 90% within a distance of 0.25 nm around peptide nonamers. P1 maybe is anchoring residue, just like P2 and P9. Distances of two Cα atoms of peptide nonamers can be utilized to check whether the structure of peptide is reasonable after molecular docking. Some high frequency residues can be applied to adjust binding subset for docking | |||
TO cite this article:Zhang Ruxiang,Huang Qinghai,Zhang Jianqiong, et al. Data analysis of HLA-A*0201 bound peptide nonamers in PDB[OL].[18 May 2006] http://en.paper.edu.cn/en_releasepaper/content/6680 |
9. The up-regulated expression of hALP gene by genotoxic agents confers cellular resistance to DNA damage | |||
Liu Haijing ,Ling Yun,Lin Hou,Zhang Bo | |||
Basic Medicine 17 March 2006 | |||
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Abstract:Objective: To confirm the response of hALP, a telomerase regulation-associated gene, to DNA damage. Methods: HeLa and Hep2 cells were respectively treated by genotoxic agents of hydrogen peroxide (H2O2) or cisplatin. The induced expression of hALP was measured by semi-quantitative RT-PCR and immunofluorescence. The transcriptional activity of hALP promoter was estimated by luciferase reporter assay. The cell survivor in the presence of genotoxic agents was analyzed by MTT method. Results: The level of hALP mRNA could be increased when treated by 0.2 ~ 1.6 mmol/L H2O2 and reach a peak in the concentration of 0.4 mmol/L. The induction could be observed after 6 h at the presence of 0.4 mmol/L H2O2 and hold high level for 36 h. Similarly, cisplatin induced hALP mRNA expression both in dose- or time-dependent. hALP protein was increased in immunofluorescent staining and concentrated in cellular nuclei with the treatment of H2O2 or cisplatin, which also stimulated the transcriptional activity of hALP promoter in luciferase assays through its upstream -705 ~ +20 nt region. On the other hand, HeLa cells expressing sense hALP gene could grow continuously in the present of 0.4 mmol/L H2O2 or 0.5µmol/L cisplatin, while cells with anti-sense hALP or control cells were slow in growth. Conclusions: The expression of hALP gene could be up-regulated by DNA damage through activating transcription of its promoter, and increase cellular resistance to genotoxic agents. | |||
TO cite this article:Liu Haijing ,Ling Yun,Lin Hou, et al. The up-regulated expression of hALP gene by genotoxic agents confers cellular resistance to DNA damage[OL].[17 March 2006] http://en.paper.edu.cn/en_releasepaper/content/5764 |
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