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	1. Fetal exposure to angiotensin II type 1 autoantibody induces hepatic insulin resistance in the adolescent offspring of rats
	WEI Mingming,ZHANG Suli,YANG Xiaoli,WANG Li,ZHAO Chengrui,LEI Jinghui,WANG Pengli,LIU Huirong
	Basic Medicine 09 February 2017
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	Abstract:Fetal origin of adult disease has gained lots of attention in relation to the occurrence of insulin resistance. Studys found offspring of pregnant rats tested positive for angiotensin II type 1 receptor autoantibody (AT1-AA) exhibited both liver damage and systemic insulin resistance during adulthood. But the mechanism and time-course associated with symptom remain unclear. Normal pregnant rats were administered with preeclampsia serum-derived AT1-AA in the second trimester to establish AT1-AA positive pregnant rat models. Compared to saline group, fasting serum glucose and insulin levels, insulin resistance index values were higher, and impaired glucose tolerance, abnormal insulin tolerance, islet compensatory hypertrophy were observed in adolescent and middle-aged offspring of AT1-AA group. Triglyceride and systolic blood pressure levels were elevated in adolescence. Hepatic glycogen synthetase reduced in the third trimester, adolescence and middle age. Expression of insulin receptor subunit, insulin receptor substrate 1/2, and their phosphoprotein decreased in hepatic insulin signaling pathway of adolescent and middle-aged offspring of AT1-AA group. We found the offspring of AT1-AA positive pregnant rats exist insulin resistance in adolescence. Meanwhile, hepatic insulin receptor and downstream receptor pathway disorder may be an important mechanism of insulin resistance in AT1-AA positive pregnant rat offspring.
	TO cite this article:WEI Mingming,ZHANG Suli,YANG Xiaoli, et al. Fetal exposure to angiotensin II type 1 autoantibody induces hepatic insulin resistance in the adolescent offspring of rats[OL].[ 9 February 2017] http://en.paper.edu.cn/en_releasepaper/content/4718781


	2. More mesenchymal stem cells are recovered from bone marrow aspirates by culturing bone marrow particles and mononuclear cells respectively
	Xing Wen,Liu Pengxia,Liu Meng,Yang Shaoguang,Zhao Qinjun,Li Jianping,Lu Shihong,Ren Hongying,Han Zhongchao
	Basic Medicine 04 January 2011
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	Abstract:Bone marrow (BM) is the major source of mesenchymal stem cells (MSCs). In most experiments, MSCs were classically cultured from mononuclear cells (MNCs) isolated by density gradient centrifugation method. However, several groups have demonstrated that this method was less efficient for MSCs' recovery. Here, we investigated whether BM particles were the cause and how to isolate them. A total of 20 patients were enrolled. MNCs were cultured by standard adherence and BM particles were cultivated by primary explant culture. For BM from patients 1-10, we first isolated MNCs, then filtered out BM particles. We then compared the morphology and the fibroblastic colony number between cultures of MNCs and BM particles. For BM from patients 11-20, we processed them in opposite order. We then compared the immunophenotype and function between adherent cells expanded from MNCs and BM particles. In addition, for patients 11-20，we cultured the left BM aspirates after BM particles and MNCs were isolated respectively. Adherent cells from BM particles were MSCs. After BM particles were filtered out and cultured separately, MSCs could be recovered completely from MNCs isolated by density gradient centrifugation and no MSCs were left in the residual BM aspirates. BM particles, which have been mostly discarded by the method of density gradient centrifugation, are another important source of MSCs and they can be cultivated reliably by primary explant culture. More MSCs are recovered from a single BM sample by culturing BM particles and MNCs respectively.
	TO cite this article:Xing Wen,Liu Pengxia,Liu Meng, et al. More mesenchymal stem cells are recovered from bone marrow aspirates by culturing bone marrow particles and mononuclear cells respectively[J].


	3. Dynamic urinary proteomic analysis
	Sun Wei ,Chen Yong ,Gao Youhe
	Basic Medicine 24 March 2009
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	Abstract:Human urinary proteome analysis is a convenient and efficient approach for understanding disease processes affecting the kidney and urogenital tract. Many potential biomarkers have been identified in previous differential analyses; however, dynamic variations of the urinary proteome have not been intensively studied, and it is difficult to conclude that potential biomarkers are genuinely associated with disease rather then simply being physiological proteome variations. In this paper, pooled and individual urine samples were used to analyze dynamic variations in the urinary proteome. Five types of pooled samples (first morning void, second morning void, excessive water-drinking void, random void, and 24 h void) collected in 1 day from six volunteers were used to analyze intra-day variations. Six pairs of first morning voids collected a week apart were used to study inter-day, inter-individual, and inter-gender variations. The intra-day, inter-day, inter-individual, and inter-gender variation analyses showed that many proteins were constantly present with relatively stable abundances, and some of these had earlier been reported as potential disease biomarkers. In terms of sensitivity, the main components of the five intra-day urinary proteomes were similar. The advantages and disadvantages of pooling samples are also discussed.
	TO cite this article:Sun Wei ,Chen Yong ,Gao Youhe . Dynamic urinary proteomic analysis [OL].[24 March 2009] http://en.paper.edu.cn/en_releasepaper/content/30668

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