Abstract:Hydroxyapatite nanoparticles is a kind of extremely development prospect of gene carrier material. In order to further improve its transfection efficiency, arginine functionalized hydroxyapatite (HAP/Arg) nanoparticles was synthesized by hydrothermal synthesis. The morphology, structure, crystallite size and Zeta potential of the HAP/Arg and its complex with DNA were characterized by transmission electron microscopy (TEM), Atomic Force Microscopy (AFM) and Zeta potential analyzer. The loading and protecting properties of HAP/Arg to DNA were tested by electrophoresis experiment. The cell viability and cytotoxicity of HAP/Arg were also performed in Hela cells and HAEC cells by MTT and LDH, and its transfection efficiency was examined by the fluorescence microscope and flow cytometry. The results reveal that the HAP/Arg is short rod and nano single crystal particles, the mean diameter of the nanoparticles is 50-90 nm and its Zeta potential is about 35.8 mV at pH of 7.4. HAP/Arg-DNA complexes can be formed by electrostatic self-assembly. The formed complex is compact with size approximately 101nm and slight positive surface charge (around +9.0 mV). What's more, it can protect DNA against degradation in DNase I and have high transfection efficiency in Hela cells with no cytotoxicity. These results suggest that the HAP/Arg nanoparticles can be a promising alternative as a novel gene delivery system.

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	1. Arginine functionalized of hydroxyapatite nanoparticles and its bioactivity for Gene Delivery
	Zhao Yanzhong,Wang Guohui,Tan Juan,Zhu Shaihong,Zhou Kechao
	Basic Medicine 18 December 2014
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	Abstract:Hydroxyapatite nanoparticles is a kind of extremely development prospect of gene carrier material. In order to further improve its transfection efficiency, arginine functionalized hydroxyapatite (HAP/Arg) nanoparticles was synthesized by hydrothermal synthesis. The morphology, structure, crystallite size and Zeta potential of the HAP/Arg and its complex with DNA were characterized by transmission electron microscopy (TEM), Atomic Force Microscopy (AFM) and Zeta potential analyzer. The loading and protecting properties of HAP/Arg to DNA were tested by electrophoresis experiment. The cell viability and cytotoxicity of HAP/Arg were also performed in Hela cells and HAEC cells by MTT and LDH, and its transfection efficiency was examined by the fluorescence microscope and flow cytometry. The results reveal that the HAP/Arg is short rod and nano single crystal particles, the mean diameter of the nanoparticles is 50-90 nm and its Zeta potential is about 35.8 mV at pH of 7.4. HAP/Arg-DNA complexes can be formed by electrostatic self-assembly. The formed complex is compact with size approximately 101nm and slight positive surface charge (around +9.0 mV). What's more, it can protect DNA against degradation in DNase I and have high transfection efficiency in Hela cells with no cytotoxicity. These results suggest that the HAP/Arg nanoparticles can be a promising alternative as a novel gene delivery system.
	TO cite this article:Zhao Yanzhong,Wang Guohui,Tan Juan, et al. Arginine functionalized of hydroxyapatite nanoparticles and its bioactivity for Gene Delivery[OL].[18 December 2014] http://en.paper.edu.cn/en_releasepaper/content/4624395


	2. Local Delivery of Flavopiridol in PLGA Nanoparticles Improves the Repair of Spinal Cord Injury by Inhibiting Astrocyte Growth and Inflammatory Factor Synthesis
	REN Hao
	Basic Medicine 25 April 2014
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	Abstract:The cell cycle inhibitor flavopiridol was previously shown in animal models to improve SCI recovery. However, the systematic dosage of flavopiridol is with side effects and the mechanism is not clear. This study aims to develop a strategy for local delivery of flavopiridol and investigate its mechanisms of function as well. Poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) was used for the sustained delivery of flavopiridol. The spinal cords were right-hemisectioned and NPs were administered into the injury site. Transparent spinal cord technology was applied for the three-dimensional observation of the anterograde tracing. The results showed that flavopiridol NPs had a susteined release of up to 3 days in vitro. Flavopiridol NPs significantly decreased inflammatory factor synthesis of astrocytes, including tnf-alpha, il-1beta, and il-6, while the il-10 expression was elevated. In-vivo study demonstrated that flavopiridol NPs decreased inflammatory expression and glial scarring, and facilitated neuronal survival and neural transmitting. Cavitation volume was decreased by proximately 90%. Administration of flavopiridol NPs also improved the motor recovery of injured animals. These findings illustrated that local delivery of flavopiridol in PLGA NPs improved SCI recovery through inhibiting astrocyte growth and inflammatory factor synthesis.
	TO cite this article:REN Hao. Local Delivery of Flavopiridol in PLGA Nanoparticles Improves the Repair of Spinal Cord Injury by Inhibiting Astrocyte Growth and Inflammatory Factor Synthesis[OL].[25 April 2014] http://en.paper.edu.cn/en_releasepaper/content/4594779

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	3. Investigation of Adhesion and Proliferation Behavior of Myoblast and Bacteria on Micro-pattern Chip
	Bao Chongyun,Xiao Yu
	Basic Medicine 06 March 2014
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	Abstract:Surface microstructure of implant materials is an essential factor to determine the adhesion and proliferation behaviors of cells and bacteria. However, it is still unclear which kind of morphological characteristics plays dominant roles. For this purpose, hydroxyapatite (HA) and polydimethylsiloxane (PDMS) chips were prepared firstly with different types of microstructures and the C2C12 cells were seeded on these chips to investigate which microstructures could affect the behavior of cells. Next, Staphylococcus aureus(S.a) and Porphyromonas gingivalis(P.g) were cultured on the PDMS matrix, respectively, to analyze the performance of bacteria on varied surface microstructures. The results illustrated much more C2C12 cells seeded on the HA chips than the PDMS replicas, indicating HA are more suitable for the cell's culture. Moreover, further observation and comparison represented both HA and PDMS samples illustrated a similar trend that the cells mainly adhered and proliferated on the submicron structure region rather than microstructure region. By contrast, the bacteria were significantly preferable to the microstructure regions. Overall, on account of different performance of cells and bacteria, we drew the conclusion that the surface roughness (RA) of implant materials should be manufactured in submicron-scale rather than micro-scale for improving the biocompatibility of biomaterials.
	TO cite this article:Bao Chongyun,Xiao Yu. Investigation of Adhesion and Proliferation Behavior of Myoblast and Bacteria on Micro-pattern Chip[OL].[ 6 March 2014] http://en.paper.edu.cn/en_releasepaper/content/4589112


	4. Effect of micro-structure on the bio-adhesion of oral bacteria
	Bao Chongyun,Xiao Yu
	Basic Medicine 06 March 2014
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	Abstract:Ihe micro-morphology of local micro-environment affects the adhesion, proliferation, differentiation and other acts of cells and microorganisms. To compare the adsorptive behavior of graphite powder and adhesive behavior of microbe on micro-structures, we prepared micro-structures on hydroxyapatite (HA) with different sizes and shapes. The results showed that Candida albicans was mostly prone to adhere onto 5 μm micro-structures, while 10-20 μm graphite powder was mostly prone onto adsorb to 10 μm and 20 μm micro-structures. The adhesion of Streptococcus mutans which grew in a chain was almost not affected by the size or shape of micro-structures, however, its proliferation was obstructed by micro-structures.
	TO cite this article:Bao Chongyun,Xiao Yu. Effect of micro-structure on the bio-adhesion of oral bacteria[OL].[ 6 March 2014] http://en.paper.edu.cn/en_releasepaper/content/4589077


	5. A Bayesian SPECT Image Reconstruction Algorithm for a High Resolution Brain Imaging System with Combined Parallel hole and Pinhole Collimation
	Huang Qiu
	Basic Medicine 27 February 2014
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	Abstract:A brain single photon emission computed tomography (SPECT) imager is simulated for obtaining high resolution brain scans for various imaging diagnostic applications such as in diagnosing brain ischemia. In this simulation the camera consists of one large field of view detector with parallel hole collimation and five smaller field of view high resolution detectors with pinhole collimation. The parallel hole collimation detector images the whole brain and acquires data without truncation. It localizes areas of particular diagnostic interest, and also provides support information for the reconstruction of data acquired by the pinhole collimation detectors. The five pinhole collimation detectors image small regions of the brain. They provide high resolution truncated projections, from which high resolution region of interest (ROI) is obtained. The reconstruction is performed using a Bayesian estimate with total variation regularization within the ROI and the low resolution image from the parallel hole collimation detector as the prior information. The camera improves the quantitation for the interior problem. The combination of large field of view parallel hole collimation detector and smaller field of view high resolution pinhole detectors improves the quantitation in simulated brain imaging. It makes use of the high sensitivity of the pinhole collimator while compensates for the degradation in the reconstructed image due to interior problem caused by the small field of view of the pinhole collimator. This work is expected to be verified through phantom data or clinical data acquired in the study of brain ischemia, where the quantitation of cerebral blood flow (CBF) and cerebral vascular reactivity (CVR) are valuable in diagnosis, and the quantitation of benzodiazepine receptor density is important in evaluating neuronal damage due to ischemic effects.
	TO cite this article:Huang Qiu. A Bayesian SPECT Image Reconstruction Algorithm for a High Resolution Brain Imaging System with Combined Parallel hole and Pinhole Collimation[OL].[27 February 2014] http://en.paper.edu.cn/en_releasepaper/content/4587320


	6. Interior SPECT Reconstruction Problem - A Prelimilary Study for High Resolution Pinhole Brain Imaging
	Huang Qiu
	Basic Medicine 27 February 2014
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	Abstract:Kudo et al. presented a unique and stable solution to the interior problem in computed tomography (CT) given tiny a proiri knowledge of the object. In this work we advance their result to the interior reconstruction problem in SPECT where a uniform attenuation map is assumed in brain imaging. In the theory, differentiation followed by backprojection (DBP) of truncated SPECT data is shown to obtain the truncated weighted Hilbert transform. Then with a proiri information on a small part of the region-of-interest (ROI), the other part of the ROI is shown to be available using the projection onto convex sets (PCOS) method.
	TO cite this article:Huang Qiu. Interior SPECT Reconstruction Problem - A Prelimilary Study for High Resolution Pinhole Brain Imaging[OL].[27 February 2014] http://en.paper.edu.cn/en_releasepaper/content/4587296


	7. Noise reduction in ventricle strain estimation based on simulated and real heart Ultrasound images
	ZHANG Yaonan,LI Sai,LI Xian,YANG Jun
	Basic Medicine 20 February 2014
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	Abstract:Cardiac resynchronization therapy (CRT) is a revolutionary treatment for heart failure diseases. Ultrasound echocardiography remains a valuable imaging technique for CRT patient selection, but it is still not accepted by the clinical society because of its inconsistent measurement results. A computational simulation could be a viable approach for validation. This paper reports our initial results of Ultrasound imaging simulation on heart. Based the simulated data and real Ultrasound images, we investigated the methods to improve the speckle tracking and strain estimation in Ultrasound echocardiography. The results turned out that a proper noise filtering and background removal could significantly improve the outputs of speckle tracking and strain estimation
	TO cite this article:ZHANG Yaonan,LI Sai,LI Xian, et al. Noise reduction in ventricle strain estimation based on simulated and real heart Ultrasound images[OL].[20 February 2014] http://en.paper.edu.cn/en_releasepaper/content/4586212


	8. Preparation of Disk-like Natural Hydroxyapatite Samples and Cell Culture on Their Surfaces
	WANG Jiandan,ZHANG Zhiwei,Lü Xiaoying
	Basic Medicine 15 January 2014
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	Abstract:The purpose of this study is to prepare natural hydroxyapatite (NHA) for biocompatibility study. The NHA was prepared from pig bones by means of a calcining procedure, and then were made into disk-like samples. Subsequently mouse mesenchymal stem cells (MSCs) were cultured in vitro and the cell viability metabolism standard curve was obtained. Finally the mouse MSCs were also tried to seed on the surface of disk-like NHA samples. The result showed NHA could be derived from pig bones by a 650 C calcining procedure. MSCs viability metabolism standard curve was obtained and proper cell density of 6000 cells per wells was selected. The cells grew well on the surface of disk-like NHA samples. These results demonstrated that NHA have good biocompatibility.
	TO cite this article:WANG Jiandan,ZHANG Zhiwei,Lü Xiaoying. Preparation of Disk-like Natural Hydroxyapatite Samples and Cell Culture on Their Surfaces[OL].[15 January 2014] http://en.paper.edu.cn/en_releasepaper/content/4582445


	9. Granger Causality relationships between SNMF components of LFPs during SD rats working memory task
	Liu Xu,Bai Wenwen,Liu Tiaotiao,Yi Hu,Tian Xin
	Basic Medicine 09 May 2013
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	Abstract:Recent studies have applied Granger causality to multivariate population recordings such as local field potentials (LFPs) or electroencephalography (EEG), which were usually observed signals. In this study, we used Granger causality to analyze relationships between source components of LFPs recorded from microelectrode arrays targeting the rats' prefrontal cortex when rats were at rest (rest group) and during Y-maze working memory task (working memory group). LFPs signals are assumed to be linear mixtures of source components. Furthermore, evidence indicates sparse coding of neural systems. Therefore, we used sparse non-negative matrix factorization (SNMF) to separate blind source components for LFPs. Then we used Granger causality to determine the strength and direction of relationships between source components.Our results showed that causal connectivity indices 'causal density' of the new networks consisting of source components strengthened than LFPs networks. By studying the new networks of rest group and working memory group, we found that the causal connectivity of working memory group was more enhanced than rest group.This paper presents a thought of applying Granger causality to source components, which may be especially useful to reveal essential causal interactions of neural networks.
	TO cite this article:Liu Xu,Bai Wenwen,Liu Tiaotiao, et al. Granger Causality relationships between SNMF components of LFPs during SD rats working memory task[OL].[ 9 May 2013] http://en.paper.edu.cn/en_releasepaper/content/4539143


	10. Triclosan-loaded PLGA microspheres-porous titanium composite coating
	DUAN Ke,XIAO Dongqin,WENG Jie
	Basic Medicine 17 April 2013
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	Abstract:Peri-implant infection remains a challenge facing joint arthroplasty, and local delivery of antibiotics is a potential solution to this problem. Here, we demonstrate a new drug-eluting coating design for antibiotic release by combining antibiotics and porous titanium (Ti). Triclosan was incorporated into poly (lactide-co-glycolide) (PLGA) microspheres, which were subsequently loaded on porous Ti coatings. Scanning electron microscopy revealed that triclosan-laden PLGA microspheres were successfully loaded onto the porous Ti and had a strong bonding with this porous coating. In vitro release study found that triclosan was released from the composite coating for 7 d. Bacterial culture indicated that the composite coating effectively killed E. coli (a model bacterial pathogen). These results may provide general coating design for implant-based release of drugs.
	TO cite this article:DUAN Ke,XIAO Dongqin,WENG Jie. Triclosan-loaded PLGA microspheres-porous titanium composite coating[OL].[17 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4536563

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