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1. The Adsorption and Release of DNA by Mesoporous Silica Materials with Different Pore Diameters. | |||
SUN Yan,YAN Tingsheng,LIU Xianbin | |||
Basic Medicine 18 March 2013 | |||
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Abstract:Safe and efficient vector systems played a crucial role in the transgenic technology. Mesoporous silica (MS) materials, which can be modified with functional groups, have the potential of being used as good vectors. In this study, the pore diameters of synthetic materials were from 2.3 nm to 5.0 nm and the samples have a good dispersion. The MS meterials with different pore diameters were successfully amino-modified and studies about the adsorption and release properties of DNA were carried out. The sample N-MCM-41-14 which had the smallest pore 2.3 nm could only adsorb DNA fragment shorter than 750 bp. The sample N-SBA-15 with 5.0 nm pore could adsorb all DNA fragment shorter than 5000 bp. The pore size of MS materials had important implications on the DNA adsorption rate and the maximum adsorption capacity. However, pore size of the materials had little effect on the release rate of DNA. In sum, MS materials with different pore diameters had different adsorption properties of DNA, and could be as prefect gene delivery systems. | |||
TO cite this article:SUN Yan,YAN Tingsheng,LIU Xianbin. The Adsorption and Release of DNA by Mesoporous Silica Materials with Different Pore Diameters.[OL].[18 March 2013] http://en.paper.edu.cn/en_releasepaper/content/4530263 |
2. The Cell Uptake of Mesoporous Silica Nanoparticles Carrying DNA in Vitro | |||
SUN Yan,LIN Li,LIU Xianbin | |||
Basic Medicine 25 February 2013 | |||
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Abstract:The application of mesoporous silica nanoparticles (MSN) in gene carrying get more and more researchers' attention with its superior properties. In this paper, we researched the two kinds of cancer cell uptake of the MSNs that carried different DNA. The small molecule nucleic acid can successfully access into the interal pore of the nanoparticles with large quantitives, and the MSNs also play a good protection effect on the DNA absorbed. Interestingly, the more quantities DNA loaded the more MSNs were internalized by cancer cells. And compare with the uptake of the HeLa cells and HepG2 cells, the overall trend were similar except a little difference about the N-MSNs and the N-MSNs loaded low concentration DNA. In addition to this the cytotoxicity of the MSNs examined by MTT assay demonstrated that the MSNs possess good biocompatibility. So the MSNs can load large quantities small molecule nucleic acid and internalized by cancer cells efficiently. The findings provide a support for clinical research of gene therapy. | |||
TO cite this article:SUN Yan,LIN Li,LIU Xianbin. The Cell Uptake of Mesoporous Silica Nanoparticles Carrying DNA in Vitro[OL].[25 February 2013] http://en.paper.edu.cn/en_releasepaper/content/4523386 |
3. Transplantation of parthenogenetic embryonic stem cells ameliorates cardiac dysfunction and remodeling after myocardial infarction | |||
Liu Yi,Ye Xiaoying,Mao Lina,Cheng Zhaokang,Yao Xinpeng,Jia Xiaohua,Mao Duo,Ou Lailiang,Li Zongjin,Che Yongzhe,Liu Na,Liu Lin,Kong Deling | |||
Basic Medicine 14 December 2012 | |||
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Abstract:Aims: Parthenogenetic embryonic stem cells (pESCs) are derived from artificially activated oocytes without fertilization and therefore raise minimal ethical concerns and may serve as attractive candidates for regenerative medicine. We thought to investigate whether pESCs can repair myocardial infarction (MI). Methods and Results: Mice (n=89) survived coronary ligation randomly received undifferentiated pESCs, ESCs, or saline. Sham-operated mice received no treatment (n=21). After 7 days, pESCs transplantation promoted pro-angiogenic factors secretion and reduced infiltrated leukocytes. pESCs-treated hearts, superior to ESC group, showed prevented cardiac remodeling and enhanced angiogenesis in and 30 days post MI. Heart contractile function was notably improved by administration of pESCs by 30 days. Moreover, tissues regenerated from the engrafted pESCs in the infarcted myocardium were positive for cardiomyocyte, endothelial cell and smooth muscle cell markers. Furthermore, teratoma fomation appeared in ESCs-treated mice in high proportion (6/34), but surprisingly not found in pESCs-treated mice (0/30) by 30 days. Conclusions: Cardiac dysfunction and adverse ventricular remodeling post MI were attenuated by pESCs transplantation, which may represent an effective and relatively safer strategy for autologous cell therapy in females. | |||
TO cite this article:Liu Yi,Ye Xiaoying,Mao Lina, et al. Transplantation of parthenogenetic embryonic stem cells ameliorates cardiac dysfunction and remodeling after myocardial infarction[OL].[14 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4504322 |
4. Introducing human Kringle 1-5 gene into MSC blocks angiogenesis | |||
LIU Haixia,CHU Yinzhu,LOU Ge | |||
Basic Medicine 19 November 2012 | |||
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Abstract:In this paper, Mesenchymal stem cell (MSC), as a cell tool, can be utilized to deliver anti-cancer molecules locally. However, MSC release some pro-angiogenic factors and may promote stem cell-mediated tumor angiogenesis. Here, we efficiently engineered human placenta MSC (HPMSC) to deliver kringle 1-5 protein (K1-5), an angiogenesis inhibitior, and conditioned medium from the expressed K1-5 protein of HPMSCS (K1-5-CM) could reduce VEGF-induced proliferation and tube formation of human umbilical veins endothelial cell (HUVEC). Moreover, Administration of K1-5-CM blocked STAT3 activation of HUVEC with VEGF stimulation and introducing K1-5 gene into HPMSC inhibited STAT3 activation and VEGF release induced by TNF-a. Take together, these data demonstrate that MSC, as cell tool of gene therapy ,engineered to express anti-angiogenesis gene not only block angiogenesis of endothelial cell in vitro but also reverse the role of MSC mediated angiogenesis and it may be a safe therapeutic option by suppressing angiogenesis in tumor. | |||
TO cite this article:LIU Haixia,CHU Yinzhu,LOU Ge. Introducing human Kringle 1-5 gene into MSC blocks angiogenesis[OL].[19 November 2012] http://en.paper.edu.cn/en_releasepaper/content/4494254 |
5. Tumor targeting polymeric micelles loaded with ultrasmall superparamagnetic iron oxide | |||
Xu Qilan,Ruan renxu,Hong Guobin | |||
Basic Medicine 03 September 2012 | |||
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Abstract:Targeted delivery of contrast agents is a highly desirable strategy for enhancing diagnostic efficiency and reducing side effects and toxicity. Water-soluble and tumor-targeting superparamagnetic iron oxide nanoparticles (SPIONs) were synthesized by loading hydrophobic SPIONs into micelles assembled from an amphiphilic block copolymer poly(ethylene glycol)-poly(ε-caprolactone) (PEG-PCL) bearing folate in the distal ends of PEG chains. Compared to the water-soluble SPIONs obtained by small molecular surfactant coating, ultrasmall SPIONs encapsulation with PEG-PCL micelles (PEG-PCL-SPIONs) simultaneously increases transversal (r2) and decreases longitudinal (r1) magnetic resonance (MR) relaxivities of water proton in micelle solution, leading to a notably high r2/r1 ratio up to 78, which makes the PEG-PCL-SPIONs a highly sensitive MRI T2 contrast agent. The mean size of folate attached SPION micelles (Fa-PEG-PCL-SPIONs) is 44 ? 3nm on average, ideal for in vivo MRI applications in which long circulation is greatly determined by small particle size and is highly desirable. Prussian blue staining of Bel 7402 cells over-expressing folate receptors, after incubation with micelle-containing medium, demonstrated that folate functionalization of the magnetic particles significantly enhanced their cell uptake. The potential of Fa-PEG-PCL-SPIONs as a potent MRI probe for in vivo tumor detection was assessed. At 3h after intravenous injection of the Fa-PEG-PCL-SPIONs solution into mice bearing subcutaneous xenografts of human Bel 7402 hepatoma , a 41.2% signal intensity decrease was detected in the T2-weighted MR images of the tumor, indicating the efficient accumulation of Fa-PEG-PCL-SPIONs in the tumor tissue. | |||
TO cite this article:Xu Qilan,Ruan renxu,Hong Guobin. Tumor targeting polymeric micelles loaded with ultrasmall superparamagnetic iron oxide[OL].[ 3 September 2012] http://en.paper.edu.cn/en_releasepaper/content/4488376 |
6. Effects of Polyethylenimines on the Morphology and Structure of Human Red Blood Cells | |||
CAI Shushan,GUO Rui,LIU Zonghua,ZHU Yi,CHEN Lili,ZHANG Yuanming | |||
Basic Medicine 15 February 2012 | |||
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Abstract:Of all synthetic polycations, polyethylenimine (PEI) is one of the most effective gene carriers in vitro and vivo because of its unique combination of high charge density and enhanced "proton spong effect" in endolysosome. When PEI is used as gene carriers in vivo, it gets access to systemic circulation sooner or later. In this perspective, it is important to study their effects on blood. The aim of this work was to report the effects of different molecular weight (MW) of PEIs on human red blood cells (RBCs), one of the blood components. Specifically, RBC hemolysis induced by PEIs was assessed, and the RBC morphology was analyzed by optical microscopy (OM), scanning electron microscopy (SEM), atomic force microscopy (AFM) and transmission electron microscopy (TEM). No obvious hemolysis was observed with PEI-0.6 and PEI-1.8 at concentrations <10 mg/mL and PEI-10 at concentrations < 0.01 mg/mL. Morphological evaluation by OM and SEM showed that, high concentrations and high MW of PEI interact with RBCs, leading to RBCs adhesion, aggregation, or both. The surface roughness of the RBCs increased along with increasing PEI-10 concentration, as observed by AFM. The internal structure of the RBCs aggregation analyzed by TEM showed that the cells aggregated and had a capacity to deform. In conclusion, PEIs caused RBC membrane disruption, morphology change, and hemolysis in a MW- and concentration-dependent manner. | |||
TO cite this article:CAI Shushan,GUO Rui,LIU Zonghua, et al. Effects of Polyethylenimines on the Morphology and Structure of Human Red Blood Cells[OL].[15 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4466685 |
7. Periodontal healing by periodontal ligament cell sheets in a teeth replantation model | |||
Zhou Yefang | |||
Basic Medicine 15 February 2012 | |||
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Abstract:Objective: Successful transplantation of avulsed teeth is to restore the attachment and regenerate the periodontal support. Different strategies have been applied in treatment from modification of teeth storage, antibiotic usage to peridontium tissue replacement. We developed a novel periodontal ligament cell-sheet delivery system to apply on delayed replanted teeth in promoting periodontal healing in a canine model. Design: Autologous periodontal ligament (PDL) fibroblasts were isolated from extracted premolars of beagle dog. The cell-sheets were fabricated using normal culture dish after stimulation of extracellular matrix formation. Teeth were surgically extracted and attached soft tissues were removed. After root canal treatment, the root of teeth were wrapped by the PDL cell-sheets and replanted back to prior socket accordingly while teeth without cell sheets as a control. Eight weeks after surgery, the animals were sacrificed and decalcified specimens were prepared. Regeneration of periodontal tissue was evaluated through histology assay. Results: Multi-layered PDL cell-sheet could be attached on tooth root and most cells on sheet-tooth constructs were viable before replantation. Minimum clinical signs of inflammation were observed in experiment. PDL cell-sheets group show significant higher occurrence of favorable healing (88.4%) than control group with low healing (5.3%). Periodontal ligament and cememtum tissue regeneration was observed in the experimental group, and the regenerated tissues showed high collagen type III, type I and fibronectin expression. Conclusion: The periodontal ligament cell-sheets fabricated through normal cell culture dish has a potential for regeneration of periodontal ligament and may become a novel therapy for avulsed teeth replantation. | |||
TO cite this article:Zhou Yefang. Periodontal healing by periodontal ligament cell sheets in a teeth replantation model[OL].[15 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4466549 |
8. Graphics processing unit cluster accelerated Monte Carlo simulation of photon transport in multi-layered tissues | |||
Jiang Chao ,He Heng ,Li Pengcheng ,Luo Qingming | |||
Basic Medicine 21 February 2011 | |||
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Abstract:In this paper, we present a GPU cluster based Monte Carlo simulation of photon transport in multi-layered tissues. The cluster is composed of multiple computing nodes in a local area network (LAN) and each node is a personal computer equipped with one or several GPU for parallel computing. In this study, the MPI (Message Passing Interface), the OpenMP (Open Multi-Processing) and the CUDA (Compute Unified Device Architecture) technologies are employed to develop the program. It is demonstrated that this designing runs roughly N-1 times faster than that using single GPU when the GPUs within the cluster are of the same type, where N is the total number of the GPUs within the cluster. | |||
TO cite this article:Jiang Chao ,He Heng ,Li Pengcheng , et al. Graphics processing unit cluster accelerated Monte Carlo simulation of photon transport in multi-layered tissues[OL].[21 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4410954 |
9. The Hierarchical Structure Depended Osteoinduction/Osteogenesis of The Hydroxyapatite Ceramics | |||
ZHI Wei,HUANG Peng,PENG Qian,ZHANG Cong,LUO Huitao,ZHANG Jingwei,HUANG Jing,WANG Hao,YU Haiyang,QU Shuxin,ZHU Zhuoli,DUAN Ke,WENG Jie,WANG Jianxin,XIA Tian,WU Dongbo | |||
Basic Medicine 14 January 2011 | |||
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Abstract:In this paper, the previously developed porous bioceramics scaffolds by accumulating hydroxyapatite (HA) spherulites which has a hierarchical structure with large dimension of 2.5~3cm in length and 1.5cm in diameter were implanted into dorsal muscle and abdominal cavity of dogs, which were deliberately created spaces within the body used as "autologous bioreactor" to grow engineered bone grafts based on the natural physiological environment. After 6 months, the dogs were sacrificed and histological analysis of explants was performed on thin paraffin sections. Lively osteogenesis was observed in pores between spherulites and in the ceramic cylinder foam, even in the micropores of HA spherulites. Bone formation by means of creeping substitution was evidently observed, in which the woven bone matrix was surrounded by osteoblasts and subsequently matured into mineralized bone while the HA bioceramics were resorbed by osteoclasts on the resorption line. Bone marrow recanalization inside the explants was obviously found. It is confirmed that an engineered bone graft similar to natural bone can been reconstructed through the novel porous bioceramics scaffold by accumulating HA spherulites in which the osteogenesis occurred in autologous bioreactors. Furthermore, the results also suggest the hierarchical structure depended osteoinduction/osteogenesis of the accumulating scaffolds. | |||
TO cite this article:ZHI Wei,HUANG Peng,PENG Qian, et al. The Hierarchical Structure Depended Osteoinduction/Osteogenesis of The Hydroxyapatite Ceramics[OL].[14 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4406625 |
10. Achieving the seeding of osteoblasts with a uniform distribution throughout the porous scaffold by accumulating hydroxyapatite spheres | |||
GUO Laiyang,ZHI Wei,ZHU Zhuoli,LUO Huitao,PENG Qian,DUAN Ke,WENG Jie,YU Haiyang,ZHANG Cong | |||
Basic Medicine 12 January 2011 | |||
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Abstract:Hydroxyapatite(HA) spheres with different diameter ranges were prepared and accumulated in a HA porous tube with dimension of 1.5 cm in length and 1.2 cm in diameter as a porous scaffold for bone tissue engineering. The porosity, macro and micro porous structures of the scaffold were characterized. Three cell seeding methods were designed to distribute osteoblasts(OBs) from Sprague-Dawley (SD) rat into HA porous scaffolds: (A) direct load, (B) admixture load, and (C) transferring load in vitro. In this study, we compared the attachment, proliferation, differentiation and homogeneity of osteoblasts seeded in the porous scaffold accumulated with HA spheres in a three-dimensional (3D) culture by the designed three cell seeding methods. The OBs/Scaffold constructs were cultured for 7 days, and then the adhesion and homogeneity of distribution, proliferation, and differentiation of osteoblasts into the osteoblastic phenotypes were determined using scanning electronic microscopy (SEM), Alamar Blue assay, and alkaline phosphatase (ALP) activity. No visible negtive effects on cell morphology, proliferation or differentiation of the porous scaffolds were observed. Among the three seeding approaches, the operation of transferring achieves the best uniform distribution and higher cell seeding efficiency of osteoblasts seeded in HA porous scaffolds. After a 7-day culture of OBs/Scaffolds, there were no considerable differences of cells proliferation or differentiation seeded by the three different methods designed. | |||
TO cite this article:GUO Laiyang,ZHI Wei,ZHU Zhuoli, et al. Achieving the seeding of osteoblasts with a uniform distribution throughout the porous scaffold by accumulating hydroxyapatite spheres[OL].[12 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4406211 |
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