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1. ACE2 mRNA expression in urinary exosome from patients of kidney disease | |||
LV Linli,CAO Yuhan,NI Haifeng,LIU Dan,MA Kunling,LIU Bicheng | |||
Clinical Medicine 13 November 2013 | |||
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Abstract:Aims: Angiotensin-converting enzyme-2 (ACE2) plays a critical role in regulation of renin-angiotensin system (RAS) and is important for the development of chronic kidney disease (CKD). ACE2 is highly expressed in tubular epithelial and glomerular cells. Urinary exosomes are microvesicles released by tubular epithelial cells and podocytes containing information of their originated cells. This study aimed to investigate expression of ACE2 mRNA in urinary exosome in different stages of CKD and to explore its correlation with renal function and histological changes. Methods: Urine samples were collected from 31 patients of chronic kidney disease(CKD) who underwent kidney biopsy and 7 controls. Urinary exosome was isolated with differential ultracentrifugation and ACE2 mRNA was detected by real-time RT-PCR on RNA isolated from urinary exosome. Results: The pellet microvesicles were positively stained with exosome marker, AQP2. The mRNA level of ACE2 showed no difference in kidney disease patients compared with controls. However, ACE2 mRNA was differently expressed in different stages of kidney disease (p=0.009 by Kruskal-Wallis test). In CKD patients with eGFR<90ml/min/1.73 m2 (n=18), ACE2 mRNA correlated with eGFR (r=-0.480, p=0.044), BUN (r=0.527, p=0.025) and score of glomerulosclerosis (r=-0.521, p=0.027). No correlation was found between ACE2 and score of tubulointerstitial fibrosis (TIF). Interestingly, in group of TIF<30%, ACE2 correlated positively with levels of glomerulosclerosis (r=0.668, p=0.007) while in group of TIF>30%, ACE2 correlated negatively with glomerulosclerosis (r=-0.663, p=0.007). In both groups, no correlation was found between eGFR and glomerulosclerosis.Conclusions: ACE2 mRNA could be readily detected in urinary exosome with different levels in different stages of CKD. It might be a novel biomarker for kidney disease reflecting the severity of glomerulosclerosis and renal function. In this paper………..(10 Points, Times New Roman) | |||
TO cite this article:LV Linli,CAO Yuhan,NI Haifeng, et al. ACE2 mRNA expression in urinary exosome from patients of kidney disease[OL].[13 November 2013] http://en.paper.edu.cn/en_releasepaper/content/4566513 |
2. Combination of antiangiogeneses effectively inhibits cancer growth and metastasis in a xenograft model | |||
HOU Li | |||
Clinical Medicine 07 April 2013 | |||
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Abstract:Angiogenesis is a cooperative process that includes endothelial growth and sprouting and interaction with the surrounding matrix. The combined use of endothelial growth inhibitor and matrix blocker expected to inhibit the angiogenesis. Antiangiogenesis is one of strategies for treating cancer. Fumagillin is an inhibitor of type 2 methionine aminopeptidase and Cyclo (Arg-Gly-Asp-D-Phe-Val), an integrin ?v?3 antagonist can block blood vessel formation. In this study, male SCID mice were injected with colon cancer cells in the subcutis and then treated with Fumagillin and Cyclo. The tumor weight, microvessel density (MVD), and number of pulmonary metastatic foci were examined. Inhibitary effect of endothelial cell growth and tube formation was examined by human umbilical endothelial cells (HUVECs), which reflect the in vivo angiogenesis. The combination of Fumagillin and Cyclo-treated mice showed the greatest effect on the tumor mass reduction, lesser number of the pulmonary metastases, and lower MVD-CD105 levels than control animals. In vitro proliferation and tube formation of HUVEC was also decreased best by the combination of both. In conclusion, The combination of Fumagillin and Cyclo at their low doses, one tenth to one hundredth was found to suppress best colorectal cancer growth and metastasis by suppressing angiogenesis. | |||
TO cite this article:HOU Li. Combination of antiangiogeneses effectively inhibits cancer growth and metastasis in a xenograft model[OL].[ 7 April 2013] http://en.paper.edu.cn/en_releasepaper/content/4535053 |
3. Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/AKT/eNOS signal transduction pathway | |||
LI Juan,QIU Fuyu,YU Lu,ZHAO Yanbo,FU Guosheng,ZHOU Binquan | |||
Clinical Medicine 15 January 2013 | |||
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Abstract:We previously showed that thymosin beta4 (Tβ4) regulates a variety of endothelial progenitor cell (EPC) functions, such as cell migration, proliferation, survival, and angiogenesis. However, the effect of Tβ4 on the senescence of circulating EPCs remains unclear. In this study, we investigated the effect of Tβ4 on EPC senescence and the signal transduction pathways involved in this process. Circulating EPCs isolated from healthy volunteers were cultured in the absence or presence of Tβ4 and various signal cascade inhibitors. Tβ4 inhibited EPC senescence in a concentration-dependent manner. Moreover, Tβ4 increased both telomerase activity and expression of telomerase reverse transcriptase (TERT) mRNA in EPCs. Tβ4 also regulated the expression of p21, p27, and cyclin D1. The effects of Tβ4 on EPC senescence were abolished by the PI3K inhibitor wortmannin and by the eNOS inhibitor L-NAME. In conclusion, the inhibitory effect on EPC senescence mediated by Tβ4 could be attributed, at least in part, to activation of the PI3K-Akt-eNOS signaling pathway. | |||
TO cite this article:LI Juan,QIU Fuyu,YU Lu, et al. Thymosin β4 reduces senescence of endothelial progenitor cells via the PI3K/AKT/eNOS signal transduction pathway[J]. |
4. How does emotional context modulate response inhibition in alexithymia:electrophysiological evidence from an ERP study | |||
ZHANG Lei,YE Rong,YU Fengqiong,CAO Zhaolun,ZHU Chunyan,CAI Zhu,HU Panpan,PU Hui,WANG Kai | |||
Clinical Medicine 18 October 2012 | |||
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Abstract:Background Alexithymia, characterized by difficulties in identifying and describing feelings, is highly indicative of a broad range of psychiatric disorders. Several studies have also discovered the response inhibition ability impairment in alexithymia. However, few studies on alexithymic individuals have specifically examined how emotional context modulates response inhibition procedure. In order to investigate emotion cognition interaction in alexithymia, we analyzed the spatiao-temporal features of such emotional response inhibition by the approaches of event-related potentials and neural source-localization. Method The study participants included 15 subjects with high alexithymia scores on the 20-item Toronto Alexithymia Scale (alexithymic group) and 15 matched subjects with low alexithymia scores (control group). Subjects were instructed to perform a modified emotional Go/Nogo task while their continuous electroencephalography activities were synchronously recorded. The task includes 3 categories of emotional contexts (positive, negative and neutral) and 2 letters ("M" and "W") centered in the screen. Participants were told to complete go and nogo actions based on the letters. We tested the influence of alexithymia in this emotional Go/Nogo task both in behavioral level and related neural activities of N2 and P3 ERP components. Results We found that negatively valenced context elicited larger central P3 amplitudes of the Nogo-Go difference wave in the alexithymic group than in the control group. Furthermore, source-localization analyses implicated the anterior cingulate cortex (ACC) as the neural generator of the Nogo-P3. Conclusion These findings suggest that difficulties in identifying feelings, particularly in negative emotions, is a major feature of alexithymia, and the ACC plays a critical role in emotion-modulated response inhibition related to alexithymia. | |||
TO cite this article:ZHANG Lei,YE Rong,YU Fengqiong, et al. How does emotional context modulate response inhibition in alexithymia:electrophysiological evidence from an ERP study[OL].[18 October 2012] http://en.paper.edu.cn/en_releasepaper/content/4491798 |
5. Discovery and identification of serum biomarkers of wilms tumor using proteomics technology | |||
JIA Zhankui,WANG Jiaxiang,YANG Jinjian,XUE Rui,ZHANG Da,WANG Guannan,MA Shengli,DUAN Zhenfeng | |||
Clinical Medicine 13 March 2012 | |||
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Abstract:Background: Wilms tumor (WT, nephroblastoma) is a cancer of the kidneys that typically occurs in children, rarely in adults. Early diagnosis of the disease is very important for its treatment and prognosis, and the aim of our study is to discover and identify potential noninvasive and convenient biomarkers for the diagnosis of this tumor. Methods: Nude mice were used to construct WT model by injecting nephroblastoma cells in their bilateral abdomen. We collected ninety four(94) serum samples from the mice for our study, including 45 WT and 49 controls. Serum proteomic profiles were analyzed using SELDI-TOF-MS. Candidate biomarkers were purified by HPLC, identified by LC-MS/MS and validated using ProteinChip immunoassays. Results: We finally retrieved two differential proteins (m/z 4509.2, 6207.9), which were identified as apolipoprotein A-II and polyubiquitin respectively. Expression of apolipoprotein A-II was higher in the WT group compared with the control group (P<0.01). In contrast, the expression of polyubiquitin was lower in the WT than in the control group. Conclusion: Apolipoprotein A-II and polyubiquitin may be used as potential biomarkers for nephroblastoma in children, and analysis of them may help us in diagnosing and treating this tumor. | |||
TO cite this article:JIA Zhankui,WANG Jiaxiang,YANG Jinjian, et al. Discovery and identification of serum biomarkers of wilms tumor using proteomics technology[OL].[13 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4470377 |
6. Current perspective on respiratory syncytial virus caused bronchiolitis | |||
Chang-Chong Li | |||
Clinical Medicine 13 March 2008 | |||
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Abstract:Bronchiolitis caused by respiratory syncytial virus (RSV) is a common lower respiratory tract infectious disease of young children. In recent years, studies on the link with bronchiolitis and asthma are payed more attention increasingly, especially on those with atopy.This review focus on the epidemiology, immunopathogenesis and development of asthma after bronchiolitis, treatmant and prevention of bronchiolitis may be involved. | |||
TO cite this article:Chang-Chong Li. Current perspective on respiratory syncytial virus caused bronchiolitis[OL].[13 March 2008] http://en.paper.edu.cn/en_releasepaper/content/19262 |
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