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1. Meta-analysis of the significance of matrix metalloproteinases for lymph node status in surgically treated NSCLC patients | |||
FAN Jiang,WU fengying,DING Jiaan,JIANG gening | |||
Clinical Medicine 21 February 2012 | |||
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Abstract:Background Matrix metalloproteinases(MMPs) are considered to be essential for estimating the metastatic potential of tumor . Various studies examining the relationship between MMPs expression with the lymph-node involvement in Non-small cell lung cancer (NSCLC)patients have yielded inconclusive results. We try to explore whether there is an expressed value of MMPs in quantitating the risk of lymphatic metastasis of NSCLC. Patients and Methods The relation between MMPs and lymph node involvement (12 studies, n = 1875 patients )was examined. Fixed or random models are applied for estimation of the summarized odds ratio and 95% confidence intervals, including a test for homogeneity of the odds ratios(OR) of the studies. Results MMP-1, -2 was significantly associated with the presence of lymph node metastasis (MMP1: OR, 0.22; 95% CI [0.13-0.37]; P<0.00001 / MMP2: OR, 0.65; 95% CI [ 0.51-0.83]; P=0.0007). MMP-7 and -9 was not (MMP7: OR, 0.81; 95% CI [0.58-1.13]; P=0.21 / MMP9: OR, 0.92; 95% CI [ 0.48-1.78]; P=0.81). Conclusions Based on current data, expression of MMP-1 and -2 in the NSCLC patients might be associated with the involvement of lymphatic metastasis while MMP-7 and-9 might not be associated with the involvement. | |||
TO cite this article:FAN Jiang,WU fengying,DING Jiaan, et al. Meta-analysis of the significance of matrix metalloproteinases for lymph node status in surgically treated NSCLC patients[OL].[21 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4467789 |
2. Synthetic Smac Peptide Enhances Chemo-sensitivity of Bladder Cancer Cells | |||
WANG Jing,ZENG Fuqing,WANG Liang,ZHU Zhaohui,JIANG Guosong | |||
Clinical Medicine 01 February 2012 | |||
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Abstract:The effects of synthetic Smac peptide (SmacN7) on chemotherapy sensitivity of bladder cancer cells were investigated.SmacN7 penetratin peptide was synthesized and delivered into T24 cells. MTT assay was used to evaluate the viability of T24 cells induced by low-dosage of MMC; Flow cytometry was used to analyze the proportions of apoptosis; Western blot was used to detect the expression of XIAP and caspase-3; The activity of caspase-3 was measured and the effect of SmacN7 combined with MMC on T24 cell lines was also determined.The results showed that SmacN7 penetratin peptide could successfully interact with endogenous XIAP, increase the proportions of apoptosis of T24 cell lines induced by low-dosage of MMC in a dose- and time-dependent manner. An obvious down-regulation of XIAP expression and up-regulation of caspase-3 was identified by Western blot. The activity of caspase-3 in experimental group was significantly increased as compared with that in the control group; Combining treated with SmacN7 penetratin peptide,the viability of T24 cells was markedly decreased to 2.22 and 3.61 fold in 24h and 48h respectively. It was concluded that SmacN7 penetratin peptide could act as a cell-permeable IAP inhibitor, inhibit the proliferation, induce apoptosis and enhance the chemo-sensitivity of bladder cancer cells to MMC. These findings indicate that SmacN7 penetratin peptide may be a very promising strategy for bladder cancer treatment when combined with chemotherapy. | |||
TO cite this article:WANG Jing,ZENG Fuqing,WANG Liang, et al. Synthetic Smac Peptide Enhances Chemo-sensitivity of Bladder Cancer Cells[OL].[ 1 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4464743 |
3. Absorption of Subdural Hematoma in a Modified Rat Model | |||
Zhang Jianning,Wang Dong | |||
Clinical Medicine 30 March 2011 | |||
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Abstract:Studies on the pathophysiology of subdural hematoma (SDH) have primarily been focused on the acute phase, wherase the course of hematoma absorption remains poorly understood. In present study, we have modified a rat model of SDH to specifically investigate the correlation between neovascularization of hematom encapsulating membrane and cerebral tissue and hematoma absorption. The modified method significant improved the success rate from 10% to 71.4% and reduced surgical related injury. Depending on this more clinically relevant model of SDH, we demonstrated a non-linear reduction in reduction volume, with an initial slow rate in the first 6 days followed by rapid absorption. The neovascularization was characterized by the formation of arachnoid granulation-like structures at the interface between hematoma and encapsulating neomembrane. The recovery of sensorimotor deficits closely correlated with the neovascularization in the neomembranes, which dictates the rate of hematoma absorption. There was a close correlation between the increase in neovascularization and the absorption rate of SDH, suggesting that the development of neovasculature in the neomembrane plays a key role in hematoma absorption and measures to enhance the process could have therapeutic potentials. | |||
TO cite this article:Zhang Jianning,Wang Dong. Absorption of Subdural Hematoma in a Modified Rat Model[OL].[30 March 2011] http://en.paper.edu.cn/en_releasepaper/content/4419224 |
4. Recipient bone marrow-derived cells contribute to neointimal formation after aortic transplantation in rat | |||
Song Zifang,Zheng Qichang,Liu Shanglong,Hu Shaobo,Li Jun,Xiong Jun,Shang Dan,Zhang Yong,Hu Qinggang | |||
Clinical Medicine 23 February 2011 | |||
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Abstract:Objective: To examine the expression of Sry gene in neointimal smooth muscle cells, detect its origin in rat aortic allograft following transplantation. Methods:Sex-mismatched bone marrow transplantation was performed from male Wistar rats to female Wistar rats, and the chimeric rat were prepared. Four weeks later, the rat aortic transplantation model was constituted by means of micro-surgery. The recipients were divided into four groups: male-male aortic isograft group, male-male aortic allograft group, female-chimera aortic allograft group and female-female aortic allograft group. Eight weeks after transplantation, aortic grafts were removed and processed. Histopathological examination and immunohistochemical staining was carried out in aortic sections. α-SMA-positive neointimal cells were harvested from cryostat sections of aortic allograft by microdissection method, and the Sry gene was amplified from the cell DNA by PCR. Results:In all aortic allografts, but not aortic isografts, α-SMA-positive smooth muscle cells were proliferated and accumulated excessively, which resulted in significant neointimal formation and vascular lumen narrowing in aortic grafts. Neointima quantitative assay revealed that the neointimal area and neointimal area/medial area ratio of grafted aorta were significantly higher in all of aortic allograft groups than that of the aortic isograft group (P<0.01), and there was no significant difference between aortic allograft groups (P>0.05). PCR amplification assay indicated that the expression of Sry gene was positive in neointimal smooth muscle cells of aortic allografts in female-chimera and male-male aortic allograft groups respectively, but not in the female-female aortic allograft group. Conclusions:As a source of neointimal smooth muscle cells, recipient bone marrow derived-cells participate in the pathological neointimal hyperplasia and allograft arteriosclerosis in rat. Recipient bone marrow-derived cells may be interesting therapeutic targets for chronic allograft vasculopathy. | |||
TO cite this article:Song Zifang,Zheng Qichang,Liu Shanglong, et al. Recipient bone marrow-derived cells contribute to neointimal formation after aortic transplantation in rat[OL].[23 February 2011] http://en.paper.edu.cn/en_releasepaper/content/4411867 |
5. Development and differentiation of neural stem cells co-cultured with epileptic neurons in vitro in rats | |||
Liu Li,Lin Zhiguo | |||
Clinical Medicine 21 January 2011 | |||
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Abstract:OBJECTIVE: To model the microenvironment in vivo, and to co-culture the NSCs with normal hippocampal neuron and "epileptic neuron" for the observation of NSCs development. DESIGN: Repeated measurement and observation. METHODS: Rat hippocampal neurons were isolated and cultured, magnesium-free media treatment was applied to establish the model of "epileptic neuron". NSCs were cultured according to regular methods. After labeled by green fluorescence protein, NSCs were co-cultured with normal hippocampal neuron or "epileptic neuron" for 14 days, respectively. Patch clamp was used to record the electrophysiology of NSCs in co-culture; immunocytochemistry was used to demonstrate the expression of synaptophysin antibody of NSCs; patch clamp was also used to record the postsynaptic potential of the neurons differentiated from NSCs in magnesium-free medium. RESULTS: After NSCs was co-cultured with normal hippocampal neuron, 14 beats/5 minutes excitatory postsynaptic potential was recorded in 60% NSCs (6/10) by patch clamp; After co-culture with "epileptic neuron", 12 beats/5 minutes excitatory postsynaptic potential of NSCs was recorded. Immunocytochemistry revealed that 80% NSCs (12/15) was observed to express the synaptophysin in co-culture with normal neuron or "epileptic neuron". In magnesium-free medium, 14 beats/5 minutes excitatory postsynaptic potential with a duration of about 10 seconds was found in 60% neurons differentiated from NSCs (9/15), and no epileptic discharge was recorded. CONCLUSION: Rat hippocampal NSCs can form functional synapse in the co-culture with "epileptic neuron" in vitro. The possibility that NSCs develop into "epileptic neuron" is minimal. | |||
TO cite this article:Liu Li,Lin Zhiguo. Development and differentiation of neural stem cells co-cultured with epileptic neurons in vitro in rats[OL].[21 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4408244 |
6. Estrogens promotes invasion of prostate cancer cells in a paracrine manner through upregulation of matrix metalloproteinase 2 in prostatic stromal cells | |||
Yu Lin ,Wang Chunyu ,Shi Jiandang ,Miao Lin ,Du Xiaoling | |||
Clinical Medicine 07 January 2011 | |||
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Abstract:Accumulating evidence suggests an enhancing effect of estrogens on prostate cancer progression. Matrix metalloproteinase 2 (MMP2), which plays an important role in prostate cancer invasion, is mainly expressed in prostatic stromal cells. Here we show that estrogen estradiol (E2) treatment upregulates MMP2 production in prostatic stromal cells, which promotes prostate cancer cell invasion in a paracrine manner. Conditioned medium (CM) was collected from estradiol (E2)-treated prostatic stromal cell line WPMY-1. The CM of E2-treated WPMY-1promoted invasion of prostate cancer (PCa) cells, as measured by Matrigel transwell assays. Treatment with E2 and PPT, an estrogen receptor-alpha (ERα) specific agonist, significantly upregulated MMP2 expression in WPMY-1 cells at both mRNA and protein levels. The CM treated with an anti-MMP2 antibody lost the stimulatory effect on invasion of PCa cells. The invasion of PCa cells were stimulated by elevated MMP2 expression induced by E2 in a paracrine manner. Our data show that estrogen estradiol induces MMP2 expression in WPMY-1 and PrSC cells. The effect of estrogen estradiol on invasion of prostate cancer cells is mediated by upregulation of MMP2 in a paracrine mechanism. | |||
TO cite this article:Yu Lin ,Wang Chunyu ,Shi Jiandang , et al. Estrogens promotes invasion of prostate cancer cells in a paracrine manner through upregulation of matrix metalloproteinase 2 in prostatic stromal cells[OL].[ 7 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4404837 |
7. In vitro pancreas duodenal homeobox-1 enhances the differentiation of pancreatic ductal epithelial cells into insulin-producing cells | |||
Liu Tao,Wang Chun-you,Yu Feng,Gou Shan-miao,Wu He-shui,Xiong Jiong-xin,Zhou Feng | |||
Clinical Medicine 08 April 2009 | |||
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Abstract:AIM: To observe whether PDX-1 enhances the differentiation of pancreatic ductal epithelial cells into insulin-producing cells in vitro. METHODS: Rat ductal epithelial cells were transfected with recombination plasmids XlHbox8VP16. After transfection, the insulin content of secretion was detected by RIA. The insulin-producing cells were detected by Dithizon-staining. RESULTS: The production and the insulin secretion of insulin-producing cells differentiated from transfected pancreatic ductal epithelial cells were higher than the untransfected in vitro with significant difference (P<0.05). CONCLUSION: PDX-1 can promote rat pancreatic ductal epithelial cells to differentiate into insulin-producing cells obviously in vitro. | |||
TO cite this article:Liu Tao,Wang Chun-you,Yu Feng, et al. In vitro pancreas duodenal homeobox-1 enhances the differentiation of pancreatic ductal epithelial cells into insulin-producing cells[OL].[ 8 April 2009] http://en.paper.edu.cn/en_releasepaper/content/31194 |
8. PDX-1 expression and significance in pancreatic cancer | |||
Liu Tao,Gou Shan-miao,Wang Chun-you,Wu He-shui,Xiong Jiong-xin,Zhou Feng | |||
Clinical Medicine 07 April 2009 | |||
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Abstract:AIM: To study the correlations of PDX-1 with pancreatic cancer characteristics. METHODS: RT-PCR and WB were used to detect PDX-1 mRNA expression in pancreatic cancer tissue and normal pancreatic tissue. Immunohistochemistry was also used to detect PCNA. RESULTS: Immunohistochemistry, WB and RT-PCR showed that 41.1% of pancreatic cancers were positive for PDX-1 expression. Lymph node metastasis (P < 0.01), TNM grading (P < 0.05), pathological grading (P < 0.05) and tumor cell proliferation (P < 0.01) were significantly correlated with PDX-1 expression levels. CONCLUSION: PDX-1-positive cells may be tumor stem cells and PDX-1 may act as alternate surface marker of pancreatic cancer stem cells. | |||
TO cite this article:Liu Tao,Gou Shan-miao,Wang Chun-you, et al. PDX-1 expression and significance in pancreatic cancer[OL].[ 7 April 2009] http://en.paper.edu.cn/en_releasepaper/content/31146 |
9. Treatment of HBV and HCV seropositive patients after renal transplantation | |||
liuhua,Xue Wujun,Tian Puxun,Ding Xiaoming,Feng Xinshun | |||
Clinical Medicine 10 February 2009 | |||
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Abstract:Backgroud/Purpose. There are higher incidence of hepatitis virus infection in renal transplant patients. In present study, we evaluated the risk and investigate the effect of integrative treatment on renal transplant patients with hepatitis B surface antigen (HBsAg) and/or seropositivity for anti-hepatitis C virus (HCV) antibody. Methods. A total of 79 HBsAg-positive and/or anti-HCV-positive patients underwent transplantation (78 kidney, one kidney and liver) between 2000 and 2007. After renal transplantation, patients with liver dysfunction received integrative therapy, including adjustment of dosage and/or type of immunosuppressants, and hepatic protection depending on the extent of the liver dysfunction. Hepatitis B virus (HBV)-DNA-positive patients were treated with lamivudine to inhibit viral replication. Results. On review of the total programme, 40 patients (50.63%) experienced liver dysfunction in the first 2 months post-operation, and five (6.33%) of these patients were HBV-DNA or HCV-RNA positive. The liver dysfunction rate was 26.09% in patients treated with tacrolimus/ mycophenolate mofetil (MMF)/prednisone (Pred), and was 60.71% in those treated with cyclosporine/MMF/Pred (P<0.05). After integrative therapy, the liver function of 36 (90%) patients was restored to normal. At the last follow-up, the survival rate of the patients and grafts was 98.48/98.48%, 92.31/92.31% and 89.26/85.71% at 1, 3 and 5 years, respective1y. Conclusion. These data suggest that timely hepatic protection and anti-viral treatment and appropriate adjustment of immunosuppressant dosage and/or type may improve the survival rate of renal transplant patients who are positive for hepatitis virus. | |||
TO cite this article:liuhua,Xue Wujun,Tian Puxun, et al. Treatment of HBV and HCV seropositive patients after renal transplantation[OL].[10 February 2009] http://en.paper.edu.cn/en_releasepaper/content/28744 |
10. Different expression of cytokine in spleen tissue and macrophage in cirrhotic patients with hypersplenism | |||
Li Zongfang,Li Aimin,Ma Shangyu,Su Qinghua,Zhang Shu,Liu Xiaogong | |||
Clinical Medicine 31 March 2008 | |||
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Abstract:Purpose: Hepatitis B virus re-infection is a difficult problem to manage after liver transplantation (LT) in patients with cirrhosis. Whether performing a splenectomy at the time of LT would be beneficial or not remains controversial. This study was designed to investigate the functional changes of splenic macrophages in cirrhotic patients with hypersplenism in order to provide additional evidences by which to assess the value of splenectomy. Methods: Fourteen cirrhotic patients with hypersplenism and six controls were enrolled in the study. Serum lipopolysaccharide (LPS) was detected with a limulus assay. The differential expression of cytokines by splenic tissue and splenic macrophages between the cirrhosis and control groups was compared with cytokine arrays. Furthermore, splenic macrophages were cultured and stimulated with LPS, after which tumor necrosis factor (TNF)-α and interleukin (IL)-12 levels in the supernatant were determined. Results: In cirrhotic patients, serum LPS levels increased significantly. Interferon (IFN)-γ, TNF-β, and transforming growth factor (TGF)-β upregulated, whereas IL-4 and IL-5 levels didn’t change in splenic tissue. TNF-α upregulated significantly, while IL-4 and IL-5 levels had no significant changes in splenic macrophages. The IL-12 levels in culture media of splenic macrophages from cirrhotic patients were significantly lower than in controls after LPS stimulation. Conclusion: Endotoxemia and predominant Th1 inflammation in splenic tissue caused splenic macrophage M1 activation in cirrhotic patients with hypersplenism, but the immune functions of splenic macrophages were impaired. | |||
TO cite this article:Li Zongfang,Li Aimin,Ma Shangyu, et al. Different expression of cytokine in spleen tissue and macrophage in cirrhotic patients with hypersplenism[OL].[31 March 2008] http://en.paper.edu.cn/en_releasepaper/content/19890 |
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