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| There are 22 papers published in subject: > since this site started. | |||
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| 1. The clinical value of basal reproductive hormones and inhibin B in AID | |||
| Huang Donghui,Hu Lian,Su Ping,Liao Aihua | |||
| Clinical Medicine 28 February 2013 | |||
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| Abstract:IObjective The present study aims to explore the clinical significance of basal reproductive hormones and basal inhibin B in predicting the outcome of AID. Materials and methods A retrospective analysis was performed in 1772 patients undergoing AID, which is being conducted at a department of assisted reproduction from 2009 to 2011. The relationships between basal reproductive hormones (FSH, lutropin, estradiol, progesterone, prolactin and testost) or basal inhibin B and pregnancy rate in AID were assessed. Results: 971 women have access to clinical pregnancy (54.80%) in 1772 women undergoing AID treatment. Found from the analysis of basal reproductive hormones and basal inhibin B shows: ①Basal FSH average in the pregnancy group ( 7.02 ±1.93 mIU / ml) were significantly lower that that in the non-pregnant group (7.27 ± 2.82 mIU/ml); the women with basal FSH ≥ 15 mIU/ml have lower clinical pregnancy rate than those women with basal FSH<15 mIU/ml (P <0.01). ②Basal inhibin B in the pregnancy group (90.61 ± 73.37 ng/ml) were significantly higher than that in the non-pregnant group (79.38 ±49.93 ng / ml); when the basal INHB≥ 25 ng/ml, the clinical pregnancy rate was significantly higher than that with basal INHB ＜25 ng/ml (P <0.05).③To combine the clinical value of ovary reserve with age, FSH* age or inhibin B/ age was used to assess the pregnancy potential. When the level FSH* age climbed above 300, the pregnancy rate decreased significantly; especially when the level of FSH* age reached above 500, the pregnancy rate (14.9%) dropped remarkably. ④With the ratio of inhibin B/age rise, the pregnancy rate was increasing. When the ratio went up to 10, the pregnancy rate was 70.59%. When the ratio inhibin B/age went down to 1, the pregnancy rate(29.3%) decreased significantly. ⑤The basal lutropin, estradiol, progesterone, prolactin and testost have no difference between the pregnancy group and non-pregnant group. Conclusion The present study concludes that basal FSH and basal inhibin B have a close correction with clinical pregnancy rate of AID, which indicate they are biochemical markers for the prediction of outcome of AID. We believe that combine the egg quantity and quanlity (such as FSH*age and inhibin B/age) provides a better tool with which to counsel subfertile couples. | |||
| TO cite this article:Huang Donghui,Hu Lian,Su Ping, et al. The clinical value of basal reproductive hormones and inhibin B in AID[OL].[28 February 2013] http://en.paper.edu.cn/en_releasepaper/content/4522676 | |||
| 2. The study of significance of endometrial carcinoma related genes and molecular phenotype | |||
| CHEN Yonghua,YAO Yuanyang,ZHANG Lili,LI Xiaoping,WANG Yue,ZHAO Lijun,WANG Jianliu,WANG Gongwei,SHEN Danhua,WEI Lihui,ZHAO Jianqing | |||
Clinical Medicine 30 January 2013
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| Abstract:In this paper, the main objective was to refine more precisely the gene expression patterns used to distinguish serous from endometrioid endometrial carcinoma and to identify the molecular phenotype of different clinicopathological subtypes of endometrial carcinoma by cDNA microarray technology. Methods: A low-density custom microarray containing 492 genes relevant to the endometrial carcinoma were used to analysis the gene expression profiles of 32 endometrioid and 5 serous endometrial cancer tissue samples. Clinicopathological characteristics among the clusters were analysised. The expression of 5 differentially expressed genes: NDC80, BUB1, FUT8, ANXA4 and BBC3 in endometrioid and serous adenocarcinoma samples was further evaluated by quantitative real-time PCR and immunohistochemistry. Results: Unsupervised cluster analysis revealed that the 5 serous adenocarcinomas clustered together. These were separated from the endometrioid adenocarcinomas which were further sorted into 3 additional clusters. A comparative analysis indicated that there was a significant difference in FIGO stage with no significant difference in depth of myometrial invasion among the 4 clusters. The FIGO ternary grading system could not distinctly separate the 3 clusters of endometrioid adenocarcinomas, but a binary grading system was able to do so. The directions of gene and protein expression change of five differentially expressed genes estimated by real-time PCR and immunohistochemistry are consistent with those estimated from microarray. Conclusions: Different clinicopathological subtypes of endometrial carcinoma exhibit distinct gene expression profiles, and a multi-protein immunohistochemistry expression pattern is constructed by selecting some candidate genes based on gene expression profiles, which may be feasible for identifying molecular phenotype in endometrial carcinoma. | |||
| TO cite this article:CHEN Yonghua,YAO Yuanyang,ZHANG Lili, et al. The study of significance of endometrial carcinoma related genes and molecular phenotype[J]. | |||
| 3. Molecular classification of human endometrial cancer based on gene expression profiles from specialized microarrays | |||
| YAO Yuanyang,CHEN Yonghua,WANG Yue,LI Xiaoping,WANG Jianliu,SHEN Danhua,WEI Lihui | |||
| Clinical Medicine 15 January 2013
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| Abstract:Objective: To investigate whether the molecular classification of endometrial cancer based on gene expression profiles can predict the biological behavior of the tumors and inform prognosis. Methods: An array containing 492 genes was used to generate gene expression profiles from 35 tumor samples. A hierarchical cluster algorithm was used to compare gene expression patterns among the tumor samples. Results: A cluster analysis revealed 3 distinct tumor clusters. A comparative analysis of tumor type, grade, FIGO stage, and depth of myometrial invasion revealed significant differences in grade and stage among the clusters, which appear to group tumors with specific clinical behaviors. Moreover, the cluster analysis initially revealed 2 clusters of differentially expressed genes. One contained 38 genes that were upregulated in most samples of the cluster representing the most advanced disease, and the other contained 27 genes that were upregulated in most samples of the cluster representing the least advanced disease. Conclusion: Molecular classification of endometrial cancer based on gene expression profiles obtained by designing specialized microarrays indicated a marked correspondence with the histologic features and clinical behavior of endometrial cancer tumors. | |||
| TO cite this article:YAO Yuanyang,CHEN Yonghua,WANG Yue, et al. Molecular classification of human endometrial cancer based on gene expression profiles from specialized microarrays[J]. | |||
| 4. Overexpression and Immunosuppressive Functions of Transforming Growth Factor β1, Vascular Endothelial Growth Factor, and Interleukin-10 in Epithelial Ovarian Cancer | |||
| Liu Chanzhen,Zhang Li,Zhang Hong,Chang Xiaohong,Cui Heng | |||
| Clinical Medicine 24 February 2012 | |||
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| Abstract:Objectives: Transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF), and interleukin-10 (IL-10) may be critical cytokines in the microenvironment of a tumor, playing roles in immune suppression. This study was conducted to elucidate the roles and immunosuppressive functions of these cytokines in epithelial ovarian cancer (EOC). Methods: The expression levels of TGF-β1, VEGF, and IL-10 in malignant tissue were evaluated by immunohistochemistry and compared with corresponding borderline, benign, and tumor-free tissue. Moreover, relationships among the levels of these cytokines and correlations between expression and the prognosis of epithelial ovarian cancer were analyzed by Pearson rank correlations and multi-factor logistic regression. The roles of TGF-β1, VEGF, and IL-10 in the immunosuppressive microenvironment of ovarian cancer were studied through dendritic cell (DC) maturation and CD4+CD25+FoxP3+ Treg generation in vitro experiments. Results: TGF-β1, VEGF, and IL-10 were expressed in 100%, 74.69%, and 54.96%, respectively, of EOC patients. TGF-β1 was an independent prognostic factor for epithelial ovarian cancer. IL-10 was significantly co-expressed with VEGF. In vitro, VEGF and TGF-1strongly interfered with DC maturation and consequently lead to immature DCs, which secreted high levels of IL-10 that accumulated around the tumor site. TGF-β1 and IL-10 induced Treg generation without antigen presentation in DCs. Conclusions: This study confirmed TGF-β1, VEGF, and IL-10 play important roles in EOC and lead to frequent immune evasion events. | |||
| TO cite this article:Liu Chanzhen,Zhang Li,Zhang Hong, et al. Overexpression and Immunosuppressive Functions of Transforming Growth Factor β1, Vascular Endothelial Growth Factor, and Interleukin-10 in Epithelial Ovarian Cancer[OL].[24 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4465648 | |||
| 5. Anti-idiotype (Id) antibody 6B11 amends the immunosuppressive state of the ovarian cancer microenvironment in vitro | |||
| Liu Chanzhen,Chang Xiaohong,CuiHeng,Zhang Hong | |||
| Clinical Medicine 24 February 2012 | |||
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| Abstract:Objectives: The goal of this investigation was to study the immune regulation and potential therapeutic value of the ovarian cancer anti-idiotype (Id) antibody 6B11.Methods: Supernatants from cultured ovarian carcinoma cell were concentrated and normalized with selected cytokines. The normalized supernatants served as an in vitro experimental model of ovarian cancer immunosuppressive microenvironments. Results:After 6B11 or its anti-anti-Id antibody Ab3 was added, the numbers of CD4+CD25+ regulatory T cells, dendritic cells, and CD8+ T cells in the model were counted by flow cytometry. The results showed that normalized supernatants of SKOV3.IP cells (TGF-beta1 > 300 pg/ml; VEGF > 6 ng/ml) markedly increased the percentage of CD4+CD25+FOXP3+ regulatory T cells and CD8+ T lymphocytes that underwent apoptosis, while the differentiation and maturation of dendritic cells was inhibited. Treatment with 20 μg/ml of 6B11 or Ab3 decreased the number of CD4+CD25+ regulatory T cells. Moreover, the number of CD83+ mature dendritic cells increased in the presence of 6B11.Conclusions: This in vitro model may serve as an optional tool for studies of ovarian cancer immunosuppressive microenvironments. treatment with 6B11 in vitro amends the immunosuppressive state of the ovarian cancer microenvironment by impacting the percentage of CD4+CD25+ regulatory T cells and the differentiation of dendritic cells. | |||
| TO cite this article:Liu Chanzhen,Chang Xiaohong,CuiHeng, et al. Anti-idiotype (Id) antibody 6B11 amends the immunosuppressive state of the ovarian cancer microenvironment in vitro[OL].[24 February 2012] http://en.paper.edu.cn/en_releasepaper/content/4465651 | |||
| 6. Variations of Human Papillomavirus Type 16 in Cervical and Skin Cancers in Sichuan, China | |||
| Xi Mingrong,Zhang Jian | |||
| Clinical Medicine 26 January 2011 | |||
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| Abstract:Human papillomavirus (HPV) is closely related with many kinds of cancers such as cervical, lung, and skin cancers, etc. HPV 16 showed intratypic variations that were known to differ in oncogenic potential and geographic distribution. This study was designed to analyze sequence variants in E6, E7 and L1 genes of HPV 16 in cervical and skin cancers in Sichuan, China. RNA or DNA extracted from 138 non-malignant controls, 122 cervical cancer patients, and 104 skin cancer patients were analyzed by PCR and direct sequencing. The infection rates of HPV 16 in cervical and skin cancers were respectively 68.9%, 5.8%, and the Asian prototype was the most prevalent variant in cervical cancer in Sichuan, China (40%). Compared with the variants detected in cervical cancer, skin cancers showed more monotonous variations, but several mutations on L1 genes existed only in skin cancers | |||
| TO cite this article:Xi Mingrong,Zhang Jian. Variations of Human Papillomavirus Type 16 in Cervical and Skin Cancers in Sichuan, China[OL].[26 January 2011] http://en.paper.edu.cn/en_releasepaper/content/4409308 | |||
| 7. CD28 IVS3 +17T>C polymorphism associated with cervical cancer risk | |||
| Chen Xiabin,Li Hua,Qiao Yan,Yu Dianke,Guo hongyan,Tan Wen,Lin Dongxin | |||
| Clinical Medicine 22 December 2010 | |||
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| Abstract:HPV plays a causal role in cervical carcinogenesis. However, only a small portion of infected individuals develop cervical cancer. Host genetic factors may confer susceptibility to this disease. CD28 participate in the maintenance of immune homeostasis as an important positive co-stimulatory molecule. Allelic variation of immuno-modulatory genes may be associated with alteration in immune function. This study is to investigate the associations between CD28 IVS3 +17T>C polymorphisms and risk of cervical cancer in Chinese population. Genotypes of CD28 polymorphisms were detected in 619 cases with primary cervical cancer and 985 normal controls. The odds ratios (ORs) and 95% confidence intervals (CIs) were calculated by unconditional logistic regression. A higher frequency of CD28 +17TC heterozygote was determined in patients in comparison with controls (19.4% versus 12.5%). Subjects carrying one CD28 IVS3 +17 C allele ( TC or CC genotype) had increased risk of cervical cancer (OR = 1.66, 95% CI, 1.27 to 2.17). This study suggests that CD28 IVS3 +17T>C polymorphism might be genetic susceptibility factor for cervical cancer in a Chinese population. | |||
| TO cite this article:Chen Xiabin,Li Hua,Qiao Yan, et al. CD28 IVS3 +17T>C polymorphism associated with cervical cancer risk[OL].[22 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4400181 | |||
| 8. Inhibitory affect of epigallocatechin gallate on ovarian cancer proliferation associated with aquaporin 5 expression | |||
| Yan Chunxiao,Yang Jianhua,Chen Xuejun | |||
Clinical Medicine 10 December 2010
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| Abstract:Objective. To investigate the effect of Epigallocatechin gallate (EGCG) on ovarian cancer cell line SKOV3 growth and aquaporin 5 (AQP5) expression as well as its possible mechanisms. Method. SKOV3 cells were treated with different concentrations of EGCG and the NF-κB-specific inhibitor pyrrolidine dithiocarbamate (PDTC) for different times. Cell proliferation was determined using the MTT assay, cell apoptosis was evaluated using the DNA ladder assay, the expression of AQP5, NF-κB p65 and IκBα was detected by immunohistochemistry, western blot analysis and RT-PCR, and the correlation of these protein expression was analyzed. Result. With increasing concentrations of EGCG and prolonged treatment times, the growth inhibition rate of SKOV3 cells gradually increased in a dose- and time-dependent manner. The expression of AQP5 and nuclear p65 and IκBα was significantly decreased (P<0.01). The cytoplasmic expression of IκBα gradually increased (P<0.05), and the apoptosis of SKOV3 cells was induced as evidenced by typical fragmentation pattern in a DNA ladder assay. With increasing concentrations of PDTC and prolonged treatment times, the protein and mRNA levels of AQP5 in SKOV3 cells decreased (P<0.01). In addition, the growth inhibition rate of SKOV3 cells significantly increased in a dose- and time-dependent manner. Conclusion. these data show that EGCG inhibited the proliferation and induced the apoptosis of ovarian cancer SKOV3 cells. EGCG also down-regulated expression of AQP5, which may inhibit tumor growth and be associated with nuclear transcription factor NF-κB. | |||
| TO cite this article:Yan Chunxiao,Yang Jianhua,Chen Xuejun. Inhibitory affect of epigallocatechin gallate on ovarian cancer proliferation associated with aquaporin 5 expression[OL].[10 December 2010] http://en.paper.edu.cn/en_releasepaper/content/4395830 | |||
| 9. Malignant ascites activates NF-κB and promotes proliferation of human ovarian carcinoma (SKOV3) cells | |||
| Yang Jianhua,Chen Xuejun,Yan Chunxiao,Shi Yifu | |||
| Clinical Medicine 28 July 2010
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| Abstract:Objective. To investigate SKOV3 cells growth induced by malignant ascites whether to involve in nuclear factor kappaB (NF-κB) signal transduction pathway. Methods. Expression of NF-κBp65 and IκBα protein of SKOV3 cells stimulated by malignant ascites, benign and borderline cystic fluid are measured by immunocytochemistry and western blotting analysis. Results. SKOV3 growth rate in the malignant ascites group (217.24±12.64) is significantly higher than that of the borderline fluid group (147.67±10.43, P=0.000), the borderline fluid group higher than the benign fluid group (57.47±2.30, P=0.000). Immunohistochemical and western blotting analyzing study show that NF-κBp65 and IκBα protein are localized in cytoplasm and karyon. Nuclear-cytosol NF-κBp65 and cytosol NF-κBp65/IκBα protein ratio in the malignant group (12.71±1.70, 3.50±0.74, respectively) is higher than that of the borderline group (2.89±0.84, P=0.000; 1.88±0.44，P=0.015, respectively), as well as the benign group (1.64±0.61, P=0.000; 1.04±0.31，P=0.001, respectively) and the control group (1.23±0.45, P=0.000; 0.69±0.09, P=0.001, respectively). There is a positively correlated relationship between expression of NF-κBp65 and IκBα (correlated coefficient 0.839, P=0.001), between SKOV3 growth rate and nuclear-cytosol NF-κBp65 protein ratio(correlated coefficient 0.878，P=0.000) as well as cytosol NF-κB/IκBα protein ratio (correlated coefficient 0.839，P=0.001). Conclusion. Malignant ascites may activate NF-κB and promote cells proliferation of human ovarian carcinoma SKOV-3 cells. | |||
| TO cite this article:Yang Jianhua,Chen Xuejun,Yan Chunxiao, et al. Malignant ascites activates NF-κB and promotes proliferation of human ovarian carcinoma (SKOV3) cells[OL].[28 July 2010] http://en.paper.edu.cn/en_releasepaper/content/4380049 | |||
| 10. Regulation of Gonadotropin-Releasing Hormone Receptor-I (GnRHR-I) Expression in Pituitary and Ovary by GnRH agonist (GnRH-a) and GnRH antagonist (GnRH-ant) | |||
| Ping Peng,Yaqin Mo,Ran Zhou,Dongzi Yang | |||
| Clinical Medicine 09 January 2009 | |||
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| Abstract: | |||
| TO cite this article:Ping Peng,Yaqin Mo,Ran Zhou, et al. Regulation of Gonadotropin-Releasing Hormone Receptor-I (GnRHR-I) Expression in Pituitary and Ovary by GnRH agonist (GnRH-a) and GnRH antagonist (GnRH-ant) [J]. | |||
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