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There are 22 papers published in subject: > since this site started. |
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1. Cloning and Transcriptional Activity of a Novel Ovarian- Specific Promoter from Rat Retrovirus-like Elements | |||
Chunhua Tu,Yaqin Mo,Weipeng Liu,Lili Huang,Dongzi Yang | |||
Clinical Medicine 09 January 2009 | |||
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Abstract:Tissue-specific promoters were genomic DNA sequences which controlled gene expression in a tissue-dependent manner and/or according to the developmental stage of the organism. The genes driven by these types of promoters would only be expressed in tissues where the gene products were desired, leaving the rest of tissues unmodified. Several promoters have been reported that could specifically control gene expression in an ovarian manner. However, most of them had shortage in research and clinical application. Recently, a family of ovarian-specific transcription units (OSTUs), which was specifically expressed in rat ovary, was identified with a probe generated from the long terminal repeat (LTR) of Moloney murine leukemia virus (MoMLV). In this study, we extracted a retrovirus-like sequence using RT-PCR according to the nucleotide sequences of these transcripts and proved it to be a novel ovarian-specific promoter by SYBR green real-time RT-PCR and transfecting reconstructed vectors, in which enhanced green fluorescence protein gene was under the control of this specific promoter, into ovarian and non-ovarian cell lines. | |||
TO cite this article:Chunhua Tu,Yaqin Mo,Weipeng Liu, et al. Cloning and Transcriptional Activity of a Novel Ovarian- Specific Promoter from Rat Retrovirus-like Elements [OL].[ 9 January 2009] http://en.paper.edu.cn/en_releasepaper/content/27568 |
2. Estradiol profiles during the luteal phase in patients undergoing IVF-ET cycles | |||
Zhongying Huang,Shangwei Li,Qianhong Ma,Lei Li,Song Jing | |||
Clinical Medicine 06 August 2007 | |||
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Abstract:To characterize the E2 profiles after oocyte pick-up (OPU) and assess the predictive value of serum E2 in early diagnosis of pregnancy following IVF-ET, in this study sixty-two patients (75 cycles involved) undergoing IVF-ET cycles whose infertility was attributed to tuble factors or male factors were enrolled. Serum E2 was detected on alternate day after OPU with micro-particle enzyme immunoassay. It was found that the level of serum E2 declined progressively after OPU in both pregnant and non-pregnant cycles and showed no significant difference on day 2, 4, 6, 8 days after OPU. In pregnant cycles following IVF-ET, serum E2 reached nadir on day 10 and then increased gradually. The differences of serum E2 level in pregnant and non-pregnant cycles were detectable from day 10 after OPU (816.4±537.6.vs 189.5±69.3 pg/ml) (P<0.05). In non-pregnant cycles, day 10 E2 in was significantly lower than that of day 8(189.5±69.3 vs 989.2±581.5 pg/ml) (P<0.05). We concluded that assessing E2 concentration on day 8 and 10 consecutively after OPU may have predictive value in diagnosing early pregnancy. When prominent drop appears, it’s very likely to see implantation failure. | |||
TO cite this article:Zhongying Huang,Shangwei Li,Qianhong Ma, et al. Estradiol profiles during the luteal phase in patients undergoing IVF-ET cycles[OL].[ 6 August 2007] http://en.paper.edu.cn/en_releasepaper/content/14397 |
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