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There are 20 papers published in subject: > since this site started. |
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1. Investigation on the wear behavior of human tooth dentin treated by gamma irradiation | |||
Ping Qing,Shengbin Huang,Shanshan Gao,Linmao Qian,Haiyang Yu | |||
Clinical Medicine 08 December 2015 | |||
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Abstract:This study aimed to evaluate the effect of gamma irradiation on the wear behavior of human tooth dentin in terms of alteration in crystallography and composition. Human premolars (n = 19) were collected to obtain perpendicular-sectioned dentin slides and parallel-sectioned dentin slides. Each slab was subjected to 2 Gy radiation dose to a cumulative dose of 60 Gy for 5 d a week, which lasted ix weeks. Nano-scratch tests were conducted on dentin surfaces with progressive loads from 0.1 mN to 80 mN. Scratch traces were observed using a scanning electron microscope and a surface profilometer. The effect of irradiation treatment on crystallography and chemical composition of dentin was investigated through X-ray diffraction and Fourier transform infrared spectroscopy. Changes in surface microhardness (SMH) were also evaluated. Nano-scratch results showed that the coefficient of friction of dentin increased after irradiation was administered; the depths and widths of the scratches were larger than those of dentin before irradiation was administered. Irradiation decreased the crystallinity of dentin and induced the formation of large crystals. The carbonate:mineral (C:M) ratio also increased. SMH significantly decreased after irradiation was administered. The main damage mechanisms involved delamination and crack formation in perpendicular-sectioned slides and parallel-sectioned slides of dentin after irradiation was administered. Therefore, irradiation directly affected the wear behavior of dentin on human tooth; irradiation also altered crystallography, chemical composition, and SMH of dentin. | |||
TO cite this article:Ping Qing,Shengbin Huang,Shanshan Gao, et al. Investigation on the wear behavior of human tooth dentin treated by gamma irradiation[OL].[ 8 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4670360 |
2. Erk1/2 Signaling Is Involved in the Differentiation of Periodontal Ligament Stem Cells to Schwann Cells in Dog | |||
LIAO Dapeng,LI Xiaojie,GONG Ping,DONG Yan,SUN Gang | |||
Clinical Medicine 22 July 2013 | |||
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Abstract:Objective: To explore the potential role of Erk1/2 signaling pathway in the differentiation of periodontal ligament stem cells (PDLSCs) to Schwann cells (SCs). Method: When PDLSCs were induced to differentiate to SCs, the cells were treated with U0126, an Erk1/2 pathway specific inhibitor, and its potential effect on SC differentiation was appraised using Western-blotting, immunostaining, and reverse transcriptase PCR.Results: Specifically inhibiting the Erk1/2 signaling pathway reduced the expression of SC-specific marker genes, S100, GFAP, and P75 and SC differentiation regulators, krox-20, Oct-6, and P0 in the cells undergoing induced differentiation from PDLSCs. Conclusion: The Erk1/2 signaling pathway is involved in the induced differentiation of PDLSCs to SCs. | |||
TO cite this article:LIAO Dapeng,LI Xiaojie,GONG Ping, et al. Erk1/2 Signaling Is Involved in the Differentiation of Periodontal Ligament Stem Cells to Schwann Cells in Dog[OL].[22 July 2013] http://en.paper.edu.cn/en_releasepaper/content/4552913 |
3. Biological behavior of neurally differentiated periodontal ligament stem cells on different titanium implant surfaces | |||
LI Xiaojie,LIAO Dapeng,GONG Ping,DONG Yan,SUN Gang | |||
Clinical Medicine 08 June 2013 | |||
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Abstract:Objective: We investigated the biological behavior of periodontal ligament stem cells (PDLSCs) induced to differentiate into Schwann cells (SCs) on the surfaces of titanium discs. Methods: Two types of titanium surfaces, sandblasted and acid-etched (SLA) and smooth polished, were prepared on titanium discs, and differentiated PDLSCs were seeded onto them. Cell morphology was examined by scanning electron microscopy, and cell proliferation was assessed using assays for MTT metabolism and total protein content. RT-PCR and Western blotting analyses were used to determine the gene and protein expression, respectively, of SC markers by PDLSCs. Results: Differentiated PDLSCs could adhere, proliferate, differentiate, and express SC marker genes and proteins on the prepared titanium surfaces, and the highest levels of SC marker protein expression were observed in PDLSCs on SLA titanium surfaces. Conclusions: SLA titanium surfaces show good biocompatibility for differentiated PDLSCs, which is important for the application of these cells in peri-implant nerve tissue engineering, through which they are expected to improve the osseoperception of dental implants. | |||
TO cite this article:LI Xiaojie,LIAO Dapeng,GONG Ping, et al. Biological behavior of neurally differentiated periodontal ligament stem cells on different titanium implant surfaces[OL].[ 8 June 2013] http://en.paper.edu.cn/en_releasepaper/content/4547249 |
4. Expression and localization of Nell-1 during murine molar development | |||
Wang Xiaoying | |||
Clinical Medicine 28 December 2012 | |||
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Abstract:Nel-like molecule-1 (Nell-1) is a recently discovered secreted protein that plays an important role in osteoblast differentiation, bone formation, and bone regeneration. However, its expression and distribution during tooth development are largely unknown. The aim of this study was to investigate the expression patterns of Nell-1 during murine molar development by immunohistochemistry. Nell-1 protein was expressed during molar development in embryonic and postnatal Kunming mice, but its expression levels and patterns at various developmental stages differed. At embryonic day 13.5 (E13.5) and E14.5, Nell-1 was found in both the entire enamel organ and the underlying mesenchyme. At E16.5, it was detected in the inner and outer enamel epithelia, stratum intermedium, secondary enamel knot, and dental papilla. At E18.5, Nell-1 was expressed in the differentiating ameloblasts, differentiating odontoblasts, and stratum intermedium. Positive staining was also found in the outer enamel epithelium. At postnatal day 2.5 (P2.5), P5, and P7, Nell-1 appeared in the secretory and mature ameloblasts and odontoblasts (odontoblastic bodies and processes) as well as immature enamel. Hertwig's epithelial root sheath also stained positively at P7. At P13.5, positive staining was restricted to the reduced dental epithelium and odontoblasts, whereas Nell-1 disappeared in the mature enamel. During tooth eruption, Nell-1 was observed only in the odontoblastic bodies, odontoblastic processes, and endothelial cells of blood vessels. The spatiotemporal expression patterns of Nell-1 during murine tooth development suggest that it might play an important role in ameloblast and odontoblast differentiation, secretion and mineralization of the extracellular enamel matrix, molar crown morphogenesis, as well as root formation. | |||
TO cite this article:Wang Xiaoying. Expression and localization of Nell-1 during murine molar development[J]. |
5. Icariin: Can an herbal extract enhance dental implant outcomes? | |||
Wang Xiaoying | |||
Clinical Medicine 20 December 2012 | |||
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Abstract:Introduction: The success rate of dental implant is closely related to three aspects including supporting bone before implantation, osseointegration process, and prevention of peri-implantitis after implantation. Icariin is one of the traditional Chinese herbal medicines to treat impotence, improve sexual function, and enhance immune function. Recently, there are abundant evidences showing that icariin can strengthen bones, enhance bone healing, inhibit osteopenia, and inhibit inflammation. The Hypothesis: We hypothesize that Icariin may be applied clinically to strengthen and accelerate osseointegration of dental implants, inhibit the peri-implantitis, and shorten the rehabilitation time. Evaluation of the Hypothesis: Limited success has been achieved to help implant surgery in spite of much effort. The application of icariin might be a potential method to improve the success rate of dental implant, contributing greatly to implant dentistry. | |||
TO cite this article:Wang Xiaoying. Icariin: Can an herbal extract enhance dental implant outcomes?[J]. |
6. Fibroblast activity on multilayer laminin5 beta3 DNA coating titanium surface | |||
JIANG Qiaohong,ZHAO Shifang,GONG Xue,HE Fuming,YANG Guoli | |||
Clinical Medicine 02 April 2012 | |||
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Abstract:This work was to investigate the fabrication of multilayer gene-functionalized films composed of cationic liposome (Lip) and pEGFP-C1-LAMB3 (pGL) DNA on titanium surfaces and the effect of the gene coatings on NIH3T3 cells. The multilayer gene coatings were fabricated on titanium by layer-by-layer (LbL) assembly technique. Surfaces analysis was carried out by contact angle measurements and X-ray photoelectron spectroscopy (XPS). Formation of LBL films was confirmed by fluorescence microscope. A biological study with NIH3T3 was followed over a 7-day culture period at days 0, 3 and 7 to observe the viability. This study showed polyelectrolyte multilayer films could be successfully deposited. EGFP expression increased with adding layer numbers. NIH3T3 adhered on multilayered DNA coatings displayed significantly higher (P<0.05) cell viability compared to control group both for 3 and 7 days culture. The results indicated the HA/pGL coatings improve the cytocompatibility of native titanium surface and presents a promising platform for delivery of DNA to NIH3T3 cells. | |||
TO cite this article:JIANG Qiaohong,ZHAO Shifang,GONG Xue, et al. Fibroblast activity on multilayer laminin5 beta3 DNA coating titanium surface[OL].[ 2 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4473769 |
7. Human Dedifferentiated Fat Cells Exhibit Perivascular Phenotype and Behaviors with Tissue-Specific Differentiation Ability | |||
Shen Jiefei,Sugawara Atsunori,Yamshita Joe,Sato Soh | |||
Clinical Medicine 03 March 2012 | |||
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Abstract:Objectives: Dedifferentiated fat (DFAT) cells derived from mature adipocytes have been considered to be a homogeneous group of multipotent cells, which might be an alternative source of adult stem cells for regenerative medicine. However, many aspects of the cellular nature about DFAT cells remained unclarified. Based on recent understandings on the connection between the tissue-specific progenitor and perivascular cells, this study aimed to elucidate the basic characteristics of DFAT cells underlying their perivascular functions and differentiation potentials. Methods and Results: By modified ceiling culture technique, DFAT cells were converted from human adipocytes in the buccal fat pads. Flow cytometry analysis revealed that those derived cells were a homogeneous population of CD13+ CD29+ CD105+ CD44+ CD140b+ CD31- CD34- CD309- CD106- CD146- α-SMA- cells. Compared with previous reports, DFAT cells in this study demonstrated tissue-specific differentiation properties with strong adipogenic but much weaker osteogenic capacity. Neither did they exhibit endothelial phenotype under angiogenic induction. For the first time, we found human DFAT cells could undertake characteristics of perivascular lineage, including expression of pericyte markers, Dil-ac-LDL uptake ability, vascular network formation ability on Matgrigel. Furthermore, DFAT cells could largely promote and stabilize the vessels structures formed by human umbilical vascular endothelial cells (HUVECs) in vitro. Conclusions: This study provided novel evidence on the pericyte nature of human DFAT cells, which also supported the recent understanding about the perivascular origin of adult stem cells that tissue-specific progenitor cells in mesenchymal tissues would associate with the blood vessels, exhibiting perivascular characteristics and functions. | |||
TO cite this article:Shen Jiefei,Sugawara Atsunori,Yamshita Joe, et al. Human Dedifferentiated Fat Cells Exhibit Perivascular Phenotype and Behaviors with Tissue-Specific Differentiation Ability[OL].[ 3 March 2012] http://en.paper.edu.cn/en_releasepaper/content/4464147 |
8. Pre-existing root cementum may promote cementum-like matrix formation in vivo by human periodontal ligament cells | |||
Song Aimei,Cai Jun,Pan Keqing,Yang Pishan | |||
Clinical Medicine 13 October 2011 | |||
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Abstract:Objective: To observe if the healthy in situ root cementum can contribute to the new cementum formation by human periodontal ligament cells (PDLCs). Methods:Root slices were made from healthy premolars.The cementum was preserved in situ in test group; while in control group , the root cementum was scaled completely. Human PDLCs were inoculated on root slices. After 7-day co-culture, 20 root slices of each group were wrapped with polytetrafluoroethylene membrane and transplanted subcutaneously into nude mice. All mice were sacrificed after eight weeks and transplanted specimens were performed for histological observation and immunohistochemistrical stain for osteopontin(OPN) and bone sialoprotein(BSP).Results: 14 of 20 specimens in the test group revealed cementum-like tissue formation on top of the old cementum and no splits were observed between them. In control group, 17 specimens showed fibrous tissue formation along the root surface and different width of splits can be seen between the new fibrous tissue and the dentin surface. Only 3 specimens demonstrated the presence of newly formed thin cementum-like tissue.The newly formed cementum-like tissue were stained positively for both OPN and BSP. Conclusions:The results showed the healthy root cementum can provide stability for the newly formed tissue and enhance the cementum-like tissue formation on top of the old cementum . It suggests that the healthy root cementum may promote the human PDLCs to differentiate towards cementoblasts and healthy root cementum should better be preserved during the periodontal regeneration procedure. | |||
TO cite this article:Song Aimei,Cai Jun,Pan Keqing, et al. Pre-existing root cementum may promote cementum-like matrix formation in vivo by human periodontal ligament cells[OL].[13 October 2011] http://en.paper.edu.cn/en_releasepaper/content/4445717 |
9. Transglutaminase 2: A Link between Periodontal Disease and Diabetes | |||
Cui Cui,Mari T. Kaartinen,Liya Chen | |||
Clinical Medicine 08 April 2010 | |||
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Abstract:Diabetes is a prevalent metabolic disease with serious complications. Studies have proclaimed a bidirectional relationship between diabetes and periodontitis, the latter resulting from chronic inflammation of tooth-supporting structures with links to cardiovascular disease and premature births. The enzyme transglutaminase 2 (TG2) has biologic functions in insulin secretion and response, and it has recently been demonstrated to play a role in the pathogenesis of diabetes. TG2 activity influences inflammatory reactions and also wound healing, collagen deposition and bone formation. We hypothesize that changes in TG2 activity may be linked to the progression of both diseases, and thus manipulation of this activity could provide a new therapeutic route for treating periodontal disease in diabetic patients. | |||
TO cite this article:Cui Cui,Mari T. Kaartinen,Liya Chen. Transglutaminase 2: A Link between Periodontal Disease and Diabetes[OL].[ 8 April 2010] http://en.paper.edu.cn/en_releasepaper/content/41689 |
10. Synchronization of vascularization and neurotization may refine tissue-engineered bone | |||
Yan xiangzhen,Yang pishan | |||
Clinical Medicine 03 December 2008 | |||
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Abstract:Although the field of bone tissue engineering has progressed significantly in recent years, the development of structurally ordered tissues has not been accomplished. The major drawbacks of current tissue-engineered bones are disorganized matrix, defective mineralization, low clinical union rate as well as bone healing rate, weak mechanical stability and a reduced osteoblast population and activity. Available studies suggest that angiogenesis is closely associated with bone resorption and bone formation. Moreover, patients with neurological disorders exhibit localized osteopenia, bone fragility and increased fracture rate. It is obvious that the phenomena present in patients with neurological disorders almost coincide with those of tissue-engineered bone. And ongoing research in angiogenesis and neurogenesis has revealed many commonalities between these processes. Therefore, we hypothesize that there is a mutual promotion process between neurogenesis and angiogenesis, and the synchronization of vascularization and neurotization will provide a necessary solution to critical problems in bone tissue engineering. From a clinical standpoint, this hypothesis may throw new light in the treatment of patients with large segmental bone defects, numerous angiogenesis-dependent diseases as well as neurodegenerative diseases. | |||
TO cite this article:Yan xiangzhen,Yang pishan. Synchronization of vascularization and neurotization may refine tissue-engineered bone[OL].[ 3 December 2008] http://en.paper.edu.cn/en_releasepaper/content/26289 |
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