Abstract:Fructose excess consumption induces insulin resistance-associated metabolic diseases. Hypothalamic insulin signaling plays a pivotal role in controlling whole-body insulin sensitivity and energy homeostasis. Wuling San and its modifications exerted various effects including anti-hypertension, anti-inflammation, anti-dyslipidemia and anti-insulin resistance. In this study, we investigated its regulatory effects on the hypothalamus of high fructose-fed mice. Mice were fed 30% fructose in drinking water for 12 weeks. After 6 weeks, these animals were orally treated with Wuling San (987, 1316, 1755 and 2340 mg/kg), allopurinol (5 mg/kg) and water daily for the next 6 weeks, respectively. Wuling San ameliorated fructose-induced hypertension, hyperlipidemia, and hyperinsulinemia and insulin resistance in mice. Wuling San ameliorated high fructose-caused hypothalamic inflammation in mice by suppressing the activation of hypothalamic nuclear factor κB (NF-κB) pathway and NOD-like receptor 3 (NLRP3) inflammasome. Wuling San also effectively restored high fructose-induced hypothalamic insulin signaling defect by up-regulating the phosphorylation of insulin receptor and protein kinase B. Allopurinol had similar effects. These results provide in vivo evidence that Wuling San-mediated inhibition of NF-κB pathway/NLRP3 inflammasome activation in the hypothalamus of mice may be associated with the reduction of hypothalamic inflammatory lesions, contributing to the improvement of hypothalamic insulin signaling defect in this model. Thus, Wuling San with the central activity may be a therapeutic for high fructose-induced metabolic syndrome in humans.

Alert Name:
Alerting to:
Authentication email will be sent to your email address in 24 hours
Frequency:
Email Message Format:	Plain text Graphical(HTML)

Complete the form below and we will recommend the selected titles to your friends on your behalf. * Indicates a required field.
Your name*:
Your email address*:
Recipient's name*:
Recipient's email address*:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find the page(s) useful.

Your name:
Your email address:
Recipient's name:
Recipient's email address:
(multiple recipient's names and email addresses should be separated with semicolons)
Your comments:	I thought you would find this page useful.

Disclaimer: This message was sent to your friend using the "Send it to a friend" facility on the Sciencepaper Online’ WWW site, http://www.paper.edu.cn/en. The Sciencepaper Online is not responsible for the content of this email, and anything said in this email does not necessarily reflect the Sciencepaper Online's views.


	1. Wuling San ameliorated hypothalamic inflammation to improve insulin signaling defect in fructose-fed mice
	Yang Ying,Zhang Dongmei,Jiao Ruiqing,Kong Lingdong,Li Jianmei
	Traditional Chinese Medicine and Pharmacology 25 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Fructose excess consumption induces insulin resistance-associated metabolic diseases. Hypothalamic insulin signaling plays a pivotal role in controlling whole-body insulin sensitivity and energy homeostasis. Wuling San and its modifications exerted various effects including anti-hypertension, anti-inflammation, anti-dyslipidemia and anti-insulin resistance. In this study, we investigated its regulatory effects on the hypothalamus of high fructose-fed mice. Mice were fed 30% fructose in drinking water for 12 weeks. After 6 weeks, these animals were orally treated with Wuling San (987, 1316, 1755 and 2340 mg/kg), allopurinol (5 mg/kg) and water daily for the next 6 weeks, respectively. Wuling San ameliorated fructose-induced hypertension, hyperlipidemia, and hyperinsulinemia and insulin resistance in mice. Wuling San ameliorated high fructose-caused hypothalamic inflammation in mice by suppressing the activation of hypothalamic nuclear factor κB (NF-κB) pathway and NOD-like receptor 3 (NLRP3) inflammasome. Wuling San also effectively restored high fructose-induced hypothalamic insulin signaling defect by up-regulating the phosphorylation of insulin receptor and protein kinase B. Allopurinol had similar effects. These results provide in vivo evidence that Wuling San-mediated inhibition of NF-κB pathway/NLRP3 inflammasome activation in the hypothalamus of mice may be associated with the reduction of hypothalamic inflammatory lesions, contributing to the improvement of hypothalamic insulin signaling defect in this model. Thus, Wuling San with the central activity may be a therapeutic for high fructose-induced metabolic syndrome in humans.
	TO cite this article:Yang Ying,Zhang Dongmei,Jiao Ruiqing, et al. Wuling San ameliorated hypothalamic inflammation to improve insulin signaling defect in fructose-fed mice[OL].[25 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4693227


	2. Using Pharmacokinetic Method to study the significance of Herbaceous compatible of peony liquorice decoction
	WANG Wenping,CAO Qichen,LI Xiaobin,WANG Huawei,YU Ming,SUI Xin,CAO Ying
	Traditional Chinese Medicine and Pharmacology 23 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Objective: To explore the rationality of herbaceous compatibility of peony liquorice decoction by peoniflorin and glycyrrhetinic acid's pharmacokinetic parameters in SD rats' plasma. Methods: A HPLC/MS/MS method was used to detect the concentration of peoniflorin and glycyrrhetinic acid in SD rats which were administrated with peony, liquorice or peony liquorice decoction separately. Results: Comparing with the single administration of peony or liquorice, the rats given with peony liquorice decoction the glycyrrhetinic acid in the plasma spent less time to reach the peak concentration and the Cmax also increased. What's more, the peoniflorin's peak concentration enhanced, and the relative bioavailability increased either. Conclusion: The liquorice promoted the absorption of peoniflorin in peony, enhanced its vitro concentration, thereby causing the peoniflorin's function stronger and more powerful. The peony has advance the time of glycyrrhetinic acid in liquorice and quantity which appears in intracorporal, causing its effect emerged rapidly.
	TO cite this article:WANG Wenping,CAO Qichen,LI Xiaobin, et al. Using Pharmacokinetic Method to study the significance of Herbaceous compatible of peony liquorice decoction[OL].[23 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4691054

Saved Papers

Saved Papers


	3. The antioxidant and antimicrobial activities of aucubin and aucubigenin
	LI Yang,ZHAO Jiawei,ZHANG Zhen,ZHAO Ye
	Traditional Chinese Medicine and Pharmacology 16 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:In this study, the iridoid glycoside aucubin from the seeds of Eucommia ulmoides Oliv and its aglycone aucubigenin have been determined for their in vitro antioxdant and antimicrobial activities. 2,2-diphenyl-2-picryhydrazyl (DPPH) radical, superoxide anion radical, and hydroxyl radical, were established to invested the antioxdant activities. The antimicrobial activities of aucubigenin and aucubin were also determined by the disc diffusion method. The results showed for the first time that the two compounds exhibited significant differences in antioxdant activities. While aucubin exhibited no antimicrobial activity, aucubigenin exhibited considerable antimicrobial activity against all of the tested bacteria.
	TO cite this article:LI Yang,ZHAO Jiawei,ZHANG Zhen, et al. The antioxidant and antimicrobial activities of aucubin and aucubigenin[OL].[16 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4691078


	4. A sensitive standardized method for determining antimicrobial activity of iridoid glycoside geniposide and its aglycone
	LI Yang,ZHANG Zhen,ZHAO Jiawei,ZHAO Ye
	Traditional Chinese Medicine and Pharmacology 15 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:The iridoid glycoside geniposide and its aglycone genipin have been investigated for their antimicrobial activities through micro-well dilution with automated spectrophotometric method, which was more accurate than paper disc diffusion method. The results showed geniposide exhibited no antimicrobial activity, while genipin showed remarkable antimicrobial activity against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica. Genipin was particularly more effective against Staphylococcus aureus with MIC value of 62.5 ug/mL and MBC value of 125 ug/mL.
	TO cite this article:LI Yang,ZHANG Zhen,ZHAO Jiawei, et al. A sensitive standardized method for determining antimicrobial activity of iridoid glycoside geniposide and its aglycone[OL].[15 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4690868


	5. Development of a LC-MS/MS Method for Determination of Triptolide in Mouse Plasma: Application to Potential Drug-drug Interaction
	ZHANG Junying,LIU Xiaoying,WU Chunyong
	Traditional Chinese Medicine and Pharmacology 12 May 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:A simple, sensitive, and accurate LC-MS/MS method for determining triptolide in mouse plasma has been developed and validated to study the drug-drug interaction with curcumin. The quantification was performed with the transitions of [M+NH4]+→[M+H]+ for triptolide. The assay for triptolide in mouse plasma was linear over the range of 0.25 to 100 ng/mL and the Lower Limit of Quantification was 0.25 ng/ml. After a single oral administration of triptolide at 0.5 mg/kg, triptolide reached the maximum exposure at 11.64 ± 4.08 min, and declined rapidly with a mean elimination half-life of 38.52 min. Co-administration of 400 mg/kg curcumin significantly increased Cmax and AUC0-∞ by 1.8-fold and 1.5-fold respectively, demonstrating that curcumin increases the bioavailability of triptolide in mouse.
	TO cite this article:ZHANG Junying,LIU Xiaoying,WU Chunyong. Development of a LC-MS/MS Method for Determination of Triptolide in Mouse Plasma: Application to Potential Drug-drug Interaction[OL].[12 May 2016] http://en.paper.edu.cn/en_releasepaper/content/4689876


	6. To Unveil the Molecular Mechanisms of Qi and Blood of Traditional Chinese Medicine through Systems Biology-Based Investigation into Si-Jun-Zi-Tang and Si-Wu-Tang formulae
	Zhang Li,Sun Jing,Kun Zhang,Wu Liping,Fan Yongsheng,Xie Zhijun
	Traditional Chinese Medicine and Pharmacology 04 April 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:[Object]To unveil potential biologic mechanisms between Qi and blood through analysis of Si-Wu-Tang (SWT) and Si-Jun-Zi-Tang (SJZT) with biomedical approaches. [Methods]Ingredients of eight herbs from SWT and SJZT were retrieved from TCMID database. The genes targeted by these ingredients were chosen according to their STITCH defined high confident (combined-score more than 0.7). We then performed enrichment analysis by Gene Ontology (GO) and DAVID. Enriched GO terms with p-value less than 0.01 were collected and interpreted the biological meanings through DAVID and KEGG. Then, a visualized network was constructed with ClueGO (a Cytoscape plug-in) to compare the difference pathways between SWT and SJZT formulae. [Results] A total of 243 genes targeted by 195 ingredients of SWT formula and 209 genes targeted by 61 ingredients of SJZT were obtained by retrieving from TCMID database. Six metabolism pathways and two environmental information processing pathways enriched by targets were correlated with two or more herbs in SWT and SJZT formula, respectively. Expect for disease associated pathways, SWT significantly influence amino acid and carbohydrate metabolism. While pathways enriched by genes targeted by ingredients of SJZT mainly belonged to organismal system function, environmental information processing, metabolism and cellular processes. The common pathways of SWT and SJZT belonged to organismal systems, metabolism, environmental information processing and cellular processes. [Conclusion]SWT with the functions of influencing amino acid and carbohydrate metabolism is significant different from SJZT with the actions of influencing neuroendocrine system by affecting serotonergic synapse to regulate thyroid hormone, ovarian steroidogenesis, prolactin and oxytocin secrction and the actions of regulating corrective hormones secretion and promoting vascular smooth muscle contraction. The common effects of SWT and SJZT are regulating the functional activities of the hypothalamic pituitary adrenal and gonadal axis by affect inhibitory synapses (GABAergic synapse) to stimulate estrogen and steroid hormones secretion and strengthening the ability of anti-infection. All the different and common pathways also reflect the characteristics of blood deficiency and Qi deficiency and indirectly reflect the molecular mechanism of blood and Qi of TCM.)
	TO cite this article:Zhang Li,Sun Jing,Kun Zhang, et al. To Unveil the Molecular Mechanisms of Qi and Blood of Traditional Chinese Medicine through Systems Biology-Based Investigation into Si-Jun-Zi-Tang and Si-Wu-Tang formulae[OL].[ 4 April 2016] http://en.paper.edu.cn/en_releasepaper/content/4682229


	7. Preparation, hydrolysis and bioactivity evaluation of a natural polysaccharide from sclerotium of Poria cocos
	Chen Qi,Yang Jia,Xu Wenjin
	Traditional Chinese Medicine and Pharmacology 08 March 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Pachyman isolated from a natural polysaccharide is lack of bioactivity due to its water-insolubility. In this study, in order to improve its bioactivity, pachyman was enzymatically hydrolyzed to obtain water soluble pachyman (WSP). Moreover, in order to further evaluate the relationship between the structure and bioactivity of pachyman, carboxymethylated pachyman (CMP) with different degree of substitution (DS) and water-soluble pachyman with different molecular weight were also prepared and their antitumor activities against S180 cells were further evaluated. Our results showed that both enzymatic hydrolysis and chemical modification were effective for improving the antitumor activity of pachyman. However, our data also suggested that the molecular weight and the degree of substitution of the derivatives should be taken into account when developing polysaccharide-based drugs.
	TO cite this article:Chen Qi,Yang Jia,Xu Wenjin. Preparation, hydrolysis and bioactivity evaluation of a natural polysaccharide from sclerotium of Poria cocos[OL].[ 8 March 2016] http://en.paper.edu.cn/en_releasepaper/content/4679252


	8. Effects of senkyunolide I on brain and plasma levels of four neurotransmitters in a rat model of migraine
	WANG Yihan,MA Qiang,FENG Yi,XU Desheng,WANG Yuan,LIANG Shuang
	Traditional Chinese Medicine and Pharmacology 14 January 2016
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Senkyunolide I is one of the major anti-migraine agents from the rhizome of Ligusticum chuanxiong Hort (chuanxiong, Umbelliferae family). Its effects on levels of β-endorphin (β-EP), endothelin-1 (ET-1), calcitonin gene-related peptide (CGRP) and 5-hydroxytryptamine (5-HT) in brain tissue and plasma were evaluated in the nitroglycerin-induced migraine rat model. Oral senkyunolide I returned elevated levels of plasma β-EP, ET-1 and CGRP to approximately those of the control group. The concentration of 5-HT in the plasma of the migraine group decreased after 240 min. In contrast, the 5-HT concentration in the plasma of the senkyunolide I group increased. The present study suggests that senkyunolide I can alter the level of β-EP, ET-1 and CGRP and 5-HT in plasma and brain tissue. This result may help to explain why senkyunolide I can reduce the pain threshold and suggests that it may be a key effective component of L. chuanxiong, which is commonly used to treat migraine.
	TO cite this article:WANG Yihan,MA Qiang,FENG Yi, et al. Effects of senkyunolide I on brain and plasma levels of four neurotransmitters in a rat model of migraine[OL].[14 January 2016] http://en.paper.edu.cn/en_releasepaper/content/4676282


	9. Research progress on Coryrlus heterophylla Fish．Leaf
	Jin zhexiong,Zhang yanping
	Traditional Chinese Medicine and Pharmacology 23 December 2015
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:Coryrlus heterophylla Fish．Leaf studies in the past 20 years were summarized. From CNKI database analysis, summarized 3 research hotspots: chemical composition, extraction and determination，feed development. It has the development and utilization prospects based on literature review in these 3 research hotspots.
	TO cite this article:Jin zhexiong,Zhang yanping. Research progress on Coryrlus heterophylla Fish．Leaf[OL].[23 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4673292


	10. Research on pharmacological effects of lycorine from Amaryllidaceae
	ZHANG Xiujuan,YU Miao,JIA Weiling,JI Yubin,ZOU Xiang,GAO Shiyong,XIN Guosong
	Traditional Chinese Medicine and Pharmacology 03 December 2015
	Show/Hide Abstract \| Cite this paper︱Full-text: PDF (0 B)

	Abstract:This paper described some pharmacological effects of lycorine from Amaryllidaceae plants. Lycorine contains a variety of unique biological activity and the pharmacological activity. New researches showed that they primarily have anti-tumor, anti-inflammatory, anti-bacterial and anti-viral as well as pharmacological activity on the central nervous system. In this paper, the pharmacological of lycorine could provide a reference for the further development and utilization of the plants in family Amarylidacae.
	TO cite this article:ZHANG Xiujuan,YU Miao,JIA Weiling, et al. Research on pharmacological effects of lycorine from Amaryllidaceae[OL].[ 3 December 2015] http://en.paper.edu.cn/en_releasepaper/content/4665614

	Check out RSS, or use RSS reader to subscribe this item