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1. Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in beer, red wine and grape juice | |||
CAO Jiliang,KONG Weijun,ZHOU Shujun,YIN Lihui,Wan Li,YANG Meihua | |||
Traditional Chinese Medicine and Pharmacology 17 January 2013 | |||
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Abstract:A simple, reliable and low-cost method based on molecularly imprinted polymer as selective sorbent of solid-phase extraction was proposed for the determination of ochratoxin A in beer, red wine and grape juice by HPLC coupled with fluorescence detection (HPLC-FLD). Samples were diluted with water and cleaned up with an AFFINIMIP? SPE OTA column. After washing and eluting, the analyte was analyzed by HPLC-FLD. Under the optimized conditions, the limit of detection (LOD) and limit of quantification (LOQ) for ochratoxin A were 0.025 ng mL?1 and 0.08 ng mL-1, respectively. The recoveries of ochratoxin A from beer, red wine and grape spiked at 0.1, 2 and 5 ng mL-1 ranged from 91.6 to 101.7%. Furthermore, after a simple regenerated procedure, the MIP-based SPE column could be reused at least fourteen times to achieve more than 80% recoveries of ochratoxin A in real samples. The developed method was applied to the detection of thirty beer, red wine and grape juice samples and just only four samples were contaminated by ochratoxin A with levels below the legal limits. | |||
TO cite this article:CAO Jiliang,KONG Weijun,ZHOU Shujun, et al. Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in beer, red wine and grape juice[J]. |
2. Anti-HIV-1 activities of extracts and phenolics from Smilax china L. | |||
WANG Weixin,QIAN Jingyi,WANG Xiaojing,JIA Aiqun | |||
Traditional Chinese Medicine and Pharmacology 09 January 2013 | |||
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Abstract:Four extracts (EtOH, CHCl3, EtOAc, and BuOH) and five phenolics (dihydrokaempferol (1), resveratrol (2), kaempferol-7-O-β-D-glucoside (3), dihydrokaempferol-3-O-α-L-rhamnoside (4), oxyresveratrol (5)) from Smilax china L. was evaluated for anti-HIV-1 activities and cytotoxicity activities in vitro. All these extracts and phenolics showed lower or no cytotoxicity at a concentration ranged from 0.8 μg/mL to 100 μg/mL, but some showed potential anti-HIV-1 activities, that is, BuOH extract and compound 2 showed higher anti-HIV-1 activities than other extracts and compounds in the tested concentrations. EtOAc extract and compound 1 and 3 showed moderate anti-HIV-1 activities at a concentration higher than 4 μg/mL. In the end, the structure-activity relationship of four extracts and five phenolics was discussed. | |||
TO cite this article:WANG Weixin,QIAN Jingyi,WANG Xiaojing, et al. Anti-HIV-1 activities of extracts and phenolics from Smilax china L.[OL].[ 9 January 2013] http://en.paper.edu.cn/en_releasepaper/content/4513713 |
3. Dual control of kidney nourishing therapy on CyclinD-CDK4/6 signal pathway of cell reproductive cycles in Lewis-bearing mice with cyclophosphamide-induced mye1osuppression. | |||
Gu Xian,Xu Zhen-ye | |||
Traditional Chinese Medicine and Pharmacology 18 December 2012 | |||
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Abstract:Background:This study investigated the dual control mechanism of kidney nourishing therapy modulating the cell cycle in Lewis-bearing mice with cyclophosphamide induced myelosuppression. Methods: Thirty Lewis-bearing mice were randomly grouped into an untreated group, control group, and treated group. Both treated and untreated groups were intraperitoneally injected with cyclophosphamide to produce a myelosuppression model. Mice in the treated group were fed with theShuanghuangShengbaigranule (40 g/day) for 6 consecutive days. Standard blood tests and the count of bone marrow nuclear cells were performed, and the cell reproductive cycles of bone marrow and tumorswere measured in these mice. In addition, the western blot approach was used to measure the upstream activating signals of CyclinD-CDK4/6 such as c-Myc and CDC25A, the upstream suppression signals such as p16INK4a and p15INK4b, and the expression of downstream activated signals such as Rb, pRB, and E2F. All of the tested results were validated by reverse transcription quantitative real-time polymerase chain reaction. Results: The results showed that the ShuanghuangShengbaigranule could elevate the count of leukocyte and bone marrow nuclear cells of Lewis-bearing mice with cyclophosphamide induced myelosuppression. It could also stimulate bone marrow cells to move from G0/G1 phases to S phase, accelerating the progress of the cell reproductive cycle and increasing the cell proliferation index. Simultaneously, the ShuanghuangShengbaigranule could also suppress cancer cells moving from G0/G1 phase to S phase, reducing the proliferation index. The tumor weight of Lewis-bearing mice in the treated group was much less than those of the control group. Expression levels of c-Myc, CDC25A, Rb, pRb, and E2F of bone marrow in ShuanghuangShengbaigranule-treated mice was higher compared to the control group, whereas they were lower in the cancer cells. Conclusion: The experimental results demonstrate that the ShuanghuangShengbaigranule has dual control on the cell reproductive cycles in cancer cells and bone marrow nuclear cells in Lewis-bearing mice. | |||
TO cite this article:Gu Xian,Xu Zhen-ye. Dual control of kidney nourishing therapy on CyclinD-CDK4/6 signal pathway of cell reproductive cycles in Lewis-bearing mice with cyclophosphamide-induced mye1osuppression.[OL].[18 December 2012] http://en.paper.edu.cn/en_releasepaper/content/4505679 |
4. Simultaneous determination of seven lignans in Justicia procumbens by high performance liquid chromatography- photodiode array detection using relative response factors | |||
LUO Zuliang,KONG Weijun,QIU Feng,YANG Meihua,LI Qian,WEI Riwei,YANG Xiaoli,QIN Jieping | |||
Traditional Chinese Medicine and Pharmacology 13 September 2012 | |||
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Abstract:A simple and sensitive high performance liquid chromatography coupled with photodiode array (HPLC-PDA) method was developed for simultaneous determination of 7 lignans in Justicia procumbens using relative response factors. The chromatographic separation was performed on a Shiseido Capcell Pak C18 column (250 × 4.6 mm id, 5 μm), a gradient elution of acetonitrile/water and a photodiode array detector. The column temperature was maintained at 35 C and the detection wavelength was set at 256 nm. Chinensinaphthol methyl ether was selected as the reference compound for calculating the relative response factors of the lignans. It has shown that the relative response factors (RRFs) for lignans are quite similar at 256 nm of detection under different analytical conditions (different columns and different HPLC instruments). Using RRFs, no lignan is needed as a reference standard, making the method ideal for rapid, routine analysis, especially for those laboratories where lignans standards are not readily available. An economic and practicable HPLC method using RRFs was established for the determination of seven lignans in J. procumbens. This method not only can determine multiple indexes in Chinese materia medica (CMM) simultaneously, but also resolve the problem of lacking of chemical standards. It will be a good quality evaluation method and pattern for CMM. | |||
TO cite this article:LUO Zuliang,KONG Weijun,QIU Feng, et al. Simultaneous determination of seven lignans in Justicia procumbens by high performance liquid chromatography- photodiode array detection using relative response factors[J]. |
5. Antitumor activity and toxicity relationship of annonaceous acetogenins in mice | |||
CHEN Yong,CHEN Jianwei,WANG Yu,XU Shasha,WANG Siliang,LI Xiang | |||
Traditional Chinese Medicine and Pharmacology 21 May 2012 | |||
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Abstract:Annonaceous acetogenins (ACGs) are one of the most interesting classes of natural products appearing in the past two decades. Here, we studied the antitumor activity and toxicity relationship of ACGs covering the three major structural classes of mono-tetrahydrofuran (THF), adjacent bis-THF and nonadjacent bis-THF compounds including annosquamin B (1), bullatacin (2) and Annosquatin B (3) in vivo. A single intraperitoneal (i.p.) injections of 100 μg/kg of 1-3 did not cause side effects in normal mice. Five doses of 25 and 50 μg/kg of 2 to H22 hepatoma cells xenografts bearing mice resulted in a above 61% reduction in tumor growth with no pathological changes, but hematologic parameters increased significantly in normal mice. Ten doses of 25, 50 and 100 μg/kg of 1 and 3 to the H22 hepatoma cells transplantation tumor model mice resulted in a maximum 53.7% and 58.7% reduction in tumor growth, respectively, and did not cause severe side effects in normal mice. This study provided the evidence that adjacent bis-THF ACGs showed higher antitumor activity, and also exhibited higher toxicity than mono-THF and nonadjacent bis-THF ACGs in vivo. | |||
TO cite this article:CHEN Yong,CHEN Jianwei,WANG Yu, et al. Antitumor activity and toxicity relationship of annonaceous acetogenins in mice[OL].[21 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4479256 |
6. Six cytoxic annonaceous acetogenins from Annona squamosa seeds | |||
CHEN Yong,CHEN Jianwei,WANG Yu,XU Shasha,LI Xiang | |||
Traditional Chinese Medicine and Pharmacology 16 May 2012 | |||
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Abstract:Custard apple (Annona squamosa L.) is an edible tropical fruit, and its seeds had been used in south China as folk medicine to treat "malignant sore" (cancer) and other usage as insecticide. Phytochemical investigation of the ethanol fraction of custard apple seeds led to the isolation of six new annonaceous acetogenins, annosquacins A-D (1-4), annosquatin A (5) and annosquatin B (6). Their structures were elucidated by spectroscopic analysis. Compounds 1-4 are adjacent bistetrahydrofuran annonaceous acetogenins. Compounds 5 and 6 are non-adjacent bistetrahydrofuran annonaceous acetogenins and the first examples in which the tetrahydrofuran ring system is located between C-9 and C-20. The absolute configurations of 1-6 were defined by the application of the Mosher method. Compounds 1-6 exhibited potent cytotoxic activity in vitro against five human tumor cell lines. 5 and 6 showed a high selectivity toward the MCF-7 and A-549 cell line respectively. | |||
TO cite this article:CHEN Yong,CHEN Jianwei,WANG Yu, et al. Six cytoxic annonaceous acetogenins from Annona squamosa seeds[OL].[16 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4478772 |
7. squamosa seeds extract containing annonaceous acetogenin compounds | |||
CHEN Yong,XU Shasha,CHEN Jianwei,WANG Yu,XU Huiqing,FAN Naibing,LI Xiang | |||
Traditional Chinese Medicine and Pharmacology 15 May 2012 | |||
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Abstract:Seeds of Annona squamosa L. have been used in the south of China as a folk remedy to treat "malignant sores" (cancer). To investigate the chemical constituents and the anti-tumor activity of the standardized A. squamosa seeds extract in vitro and in vivo. Annonaceous acetogenin profiles of the standardized extract were determined by using Fourier transform infrared (FT-IR) and high performance liquid chromatography (HPLC) techniques. The anti-tumor activity of the extract was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cytotoxicity in vitro and H22 hepatoma cells transplantation tumor model in vivo. The FT-IR spectroscopy showed the presence of annonaceous acetogenin compounds in the extract. Two major annonaceous acetogenins: 12, 15-cis-squamostatin-A and bullatacin were identified and quantified by HPLC. The seed extract showed significant anti-tumor activity against four human tumor cell lines, especially for MCF-7 (IC50. 0.25 μg/ml) and Hep G2 (IC50. 0.36 μg/ml) cells in vitro. The extract inhibited the growth of H22 tumor cells in mice with a maximum inhibitory rate of 69.55% by oral administration. A. squamosa seed extract showed significant anti-tumor activities against human hepatoma cells in vitro and in vivo, indicating a potential for developing the extract as a novel anti-liver cancer drug. | |||
TO cite this article:CHEN Yong,XU Shasha,CHEN Jianwei, et al. squamosa seeds extract containing annonaceous acetogenin compounds[OL].[15 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4478623 |
8. Novel materials which possess the ability to target liver cells | |||
Chen Zhipeng,Xiao Lu,Cai Baochang | |||
Traditional Chinese Medicine and Pharmacology 26 April 2012 | |||
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Abstract:Introduction: Hepatic-targeted drug delivery systems are designed to treat diseases of the liver. However, since there are several different types of liver diseases that are caused by different cells, it is important to select the proper materials to target these different cells.Areas covered: This review addresses novel materials that possess the ability to target liver cells via receptor--ligand processes and offers an insight into the aspects of formulation design. It also discusses several approaches used to enhance the targeting efficiency of drug delivery systems to receptors in the liver cells. In addition, the delivery efficiency and therapeutic efficacy of these materials in the treatment of acute or chronic liver diseases is highlighted. Expert opinion: Further research into the use of clinical materials and the design ofsmartmaterials formulti-drug delivery todifferent organelles is important for future studies on these new materials. It is hoped that these targeted therapeutics will benefit patients with liver disorders in the near future. | |||
TO cite this article:Chen Zhipeng,Xiao Lu,Cai Baochang. Novel materials which possess the ability to target liver cells[OL].[26 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4476385 |
9. Monodisperse core-shell microstructured molecular imprinting silica for highly selective enrichment of isoflavonoid glycosides from Radix Puerariae | |||
Jing Luo,Qing Zhang,Liying Zhang,Jiawei Zhao,Shuhu Du | |||
Traditional Chinese Medicine and Pharmacology 20 April 2012 | |||
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Abstract:This paper reports the preparation of puerarin (PR) imprinted layer-coated silica microparticles toward selective recognition of PR and fast affinity-enrichment of the main isoflavonoid glycosides from the crude extract of Radix puerariae. Before the preparation, quantum mechanics (QM) method was applied to identify three kinds of common functional monomers capable of interaction with PR and then predict optimal functional monomer (acrylamide, AA) and the relative molar ratio of template to functional monomer (PR/AA, 1 : 4). The obtained PR-imprinted silica microparticles were evaluated by transmission electron microscope (TEM) and rebinding experiments, exhibiting good morphology and high binding affinity to PR. Meanwhile, the rebinding amount of the imprinted microparticles to PR was nearly 2.1-folds that of non-imprinted microparticles. When the PR-imprinted microspheres were used as packing materials for solid-phase extraction, the recovery yields of PR, daidzin (DD) and genistin (GS) were simultaneously up to 90% by one-step extraction from the crude extract of Radix puerariae. Additionally, the PR-imprinted microparticles could be re-used for at least 5 times without losing any extraction efficiency. These results indicate that the PR-imprinted microparticles have highly selective adsorption capabilities to PR, DD and GS from the crude extract of Radix puerariae. The method of molecularly imprinted polymers (MIPs) coupled with solid-phase extraction (SPE) provides a good solution of the enrichment and separation of active extracts from complicated traditional Chinese medicine (TCM) with certain structures. | |||
TO cite this article:Jing Luo,Qing Zhang,Liying Zhang, et al. Monodisperse core-shell microstructured molecular imprinting silica for highly selective enrichment of isoflavonoid glycosides from Radix Puerariae[OL].[20 April 2012] http://en.paper.edu.cn/en_releasepaper/content/4476028 |
10. Effect of solanine on cell apoptosis by regulating Bcl-2/Bax ratio in HepG2 cell | |||
JI Yubin,GAO Shi-yong | |||
Traditional Chinese Medicine and Pharmacology 10 January 2012 | |||
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Abstract:Objective To explore the anti-tumor effect of solanine and the mechanisms involved. Methods The in vivo anti-tumor effect of solanine is observed using models developed through in vivo transplantation of tumor cells; in vitro lines of sensitive anti-tumor cells are selected from the digestive system using MTT assay; the effect of solanine on cell morphology is observed using transmission electronic microscopy; the morphology of apoptotic cells is observed using Annexin V/PI double staining and laser confocal scanning microscopy; the rate of cell apoptosis is measured using Annexin V/PI double staining and flow cytometry; the concentration of intracellular Ca2+ is determined using Fluo-3/AM staining and laser confocal scanning microscopy; the membrane potential of cellular mitochondria is determined using TMRE staining and laser confocal scanning microscopy; the protein expression of Bcl-2 and Bax is measured using immunological marking and laser confocal scanning microscopy; and the activity of caspase-3 is measured using the colorimetric method. Results Solanine can inhibit the growth of tumor weight in S180 tumor-bearing mice and prolong the survival time of H22 tumor-bearing mice. Selection by MTT assay reveals that HepG2 cells are quite sensitive to solanine, for solanine can induce morphological changes characteristic of apoptotic cells in HepG2 cells, with the rate of early apoptosis being 4%, 8.5%, and 20.1%, for HepG2 cells treated for 24 h with solanine of concentrations of 0.4, 2, and 10μg, respectively. Solanine can raise the concentration of intracellular Ca2+ and lower the membrane potential. It can reduce the protein expression of Bcl-2 while increasing that of Bax, thus increasing the activity of caspase-3. Conclusion Solanine demonstrates clear anti-tumor activity, inhibiting human hepatocarcinoma. This inhibitory effect is achieved through solanine’s decreasing the Bcl-2/Bax ratio, thus increasing the concentration of intracellular Ca2+, which turns on the enzymatic activity of the caspase family, thus inducing the apoptosis of HepG2 cells. | |||
TO cite this article:JI Yubin,GAO Shi-yong. Effect of solanine on cell apoptosis by regulating Bcl-2/Bax ratio in HepG2 cell[OL].[10 January 2012] http://en.paper.edu.cn/en_releasepaper/content/4458765 |
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