Abstract:The aim of the present study was to evaluate the diuretic effect on the urinary electrolyte concentration(Na+, K+, Cl-) and regulation of the relative mRNA expression of aquaporin-1(AQP1), aquaporin-2(AQP2), aquaporin-3(AQP3) and vasopressin V2 receptor (V2R)post oral administration of sclerotia of P.umbellata aqueous extract in normal rats. Aqueous extract of sclerotia of Polyporus umbellatus (50mg/kg, 250mg/kg, 500mg/kg) or the reference drug, furosemide (10mg/kg) were administrated orally to male SD rats and their urine output was quantified and collected 24h and 8days after the treatment. The kidney medulla AQP1, AQP2, AQP3 and V2R mRNA relative expressions were measured with RT-PCR. After single dose of the exact of sclerotia of P.umbellata, urine output was found to be significantly increased, which began at 4h, and at 24h after the treatment, the sclerotia of Polyporus umbellatus extract and furosemide treatment produced the similar total volume of urine excreted. The extract increase urinary levels of Na+, K+, Cl-, to about the same extent, while furosemide increased urinary levels of Na+ and Cl-. After the 8-day doses, all two substances induced significant diuresis, natriuresis and chloriuresis. These two substances don’t regulate the AQP1 and AQP3 mRNA level in normal rat kidney medulla. The AQP2 mRNA level of sclerotia of P.umbellata extract was down-regulated significantly, the V2R mRNA level of sclerotia of P.umbellata extract 50mg/kg dose group and 250mg/kg dose group were down-regulated significantly too. Interestingly, the low-dose group had higher effect on regulation of AQP2 and V2R mRNA level. Aqueous extract of sclerotia of Polyporus umbellatus has conspicuous diuretic effect confirming its ethnopharmacological use. From the pattern of excretion of water, sodium, potassium, cholorine, AQP2 and V2R mRNA level, it may be logically concluded that it has effect from down-regulating AQP2, and down-regulate AQP2 by down-regulating V2R.

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	1. Diuretic activity and kidney medulla AQP1, AQP2, AQP3, V2R expression of the aqueous extract of sclerotia of Polyporus umbellatus FRIES in normal rats
	Guowei Zhang,Xing Zeng,Han Ling,Wei Jian＇an,Hang Haiding
	Traditional Chinese Medicine and Pharmacology 17 November 2009
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	Abstract:The aim of the present study was to evaluate the diuretic effect on the urinary electrolyte concentration(Na+, K+, Cl-) and regulation of the relative mRNA expression of aquaporin-1(AQP1), aquaporin-2(AQP2), aquaporin-3(AQP3) and vasopressin V2 receptor (V2R)post oral administration of sclerotia of P.umbellata aqueous extract in normal rats. Aqueous extract of sclerotia of Polyporus umbellatus (50mg/kg, 250mg/kg, 500mg/kg) or the reference drug, furosemide (10mg/kg) were administrated orally to male SD rats and their urine output was quantified and collected 24h and 8days after the treatment. The kidney medulla AQP1, AQP2, AQP3 and V2R mRNA relative expressions were measured with RT-PCR. After single dose of the exact of sclerotia of P.umbellata, urine output was found to be significantly increased, which began at 4h, and at 24h after the treatment, the sclerotia of Polyporus umbellatus extract and furosemide treatment produced the similar total volume of urine excreted. The extract increase urinary levels of Na+, K+, Cl-, to about the same extent, while furosemide increased urinary levels of Na+ and Cl-. After the 8-day doses, all two substances induced significant diuresis, natriuresis and chloriuresis. These two substances don’t regulate the AQP1 and AQP3 mRNA level in normal rat kidney medulla. The AQP2 mRNA level of sclerotia of P.umbellata extract was down-regulated significantly, the V2R mRNA level of sclerotia of P.umbellata extract 50mg/kg dose group and 250mg/kg dose group were down-regulated significantly too. Interestingly, the low-dose group had higher effect on regulation of AQP2 and V2R mRNA level. Aqueous extract of sclerotia of Polyporus umbellatus has conspicuous diuretic effect confirming its ethnopharmacological use. From the pattern of excretion of water, sodium, potassium, cholorine, AQP2 and V2R mRNA level, it may be logically concluded that it has effect from down-regulating AQP2, and down-regulate AQP2 by down-regulating V2R.
	TO cite this article:Guowei Zhang,Xing Zeng,Han Ling, et al. Diuretic activity and kidney medulla AQP1, AQP2, AQP3, V2R expression of the aqueous extract of sclerotia of Polyporus umbellatus FRIES in normal rats[J].J Ethnopharmacol ，2010年，128卷，2期，433 ~ 437


	2. Explore the new idea for evaluation on the efficacy of Ethnomedicine
	Zhao Qipeng,Wang Zhang,Wang Shu,Zhang Yi
	Traditional Chinese Medicine and Pharmacology 22 June 2009
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	Abstract:Take Tibetan, Mongolian, Dai, Miao medicine for example in this paper,discuss its research Status and deficiency of evaluation on efficacy currently.Put forward the new idea reflecting the systematic evaluation on the effectiveness of ethnomedicine under the guidance of the overall concept of the Ethnomedicine theory. That is based on the system design ideas， such as systems biology and systems chemical biology, take use of modern analytical techniques (such as metabolomics) and other techniques to revealing the relationship between chemical composition, biological effects, metabolic markers. It is an important idea for the modern ethnomedicine research and development of new drugs from ethnomedicine.
	TO cite this article:Zhao Qipeng,Wang Zhang,Wang Shu, et al. Explore the new idea for evaluation on the efficacy of Ethnomedicine [OL].[22 June 2009] http://en.paper.edu.cn/en_releasepaper/content/33295

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	3. Neuroprotective Effects of Regulating the Blood Ocean in Brain Method and BMSCs Transplantation in Ischemia-reperfusion Rats
	Guo Jianwen,Li Jun-ya,Huang Yan
	Traditional Chinese Medicine and Pharmacology 12 March 2009
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	Abstract:Objective To observe the effect of the Chinese medicine naomaiyihao capsule which can regulate the blood ocean in brain and Bone Marrow Mesenchyrmal Stem Cells(BMSCs) transplantation in the treatment of cerebral ischemia adult rats moda1.Methods：Preparation of rat brain ischemia reperfusion injury were randomly divided into sham group, model group, BMSCs transplantation group, naomaiyihao group and combined treatment group to observe the rats symptoms of neurological damage and brain cells change shape Results neurological damage and brain tissue were significantly alleviated in combined treatment group than those in other groups. Conclusion naomaiyihao could ease the neurological injury of Cerebral ischemia-reperfusion injury rats through the promotion of BMSCs to the differentiation of neurons, played a role in nerve protection.
	TO cite this article:Guo Jianwen,Li Jun-ya,Huang Yan. Neuroprotective Effects of Regulating the Blood Ocean in Brain Method and BMSCs Transplantation in Ischemia-reperfusion Rats[OL].[12 March 2009] http://en.paper.edu.cn/en_releasepaper/content/30179


	4. Investigation of the Lower Resistance Meridian V. Speculation on the Pathophysiological Functions of Acupuncture Meridians
	Weisheng Yang
	Traditional Chinese Medicine and Pharmacology 18 November 2008
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	Abstract:It was pointed out in the preceding two papers of the present series that the meridians are only zones in the loose connective tissue but containing richer interstitial fluid, and thus are lower-resistance passages for diffusion of meridian-signal carriers or mediators. Moreover, a hypothesis, which incorporates the wide variety of functions of the loose connective tissue, the circulatory system, and the nervous system into the meridian function, has been proposed and in the hypothesis the mast cell plays some key roles. In the present paper, considering also the latest knowledge on cell migration along with some existing experimental results, it is further pointed out that meridians ought to be lower-resistance passages for chemotactic migration of cells and mast cells can indeed migrate longitudinally along meridians. Finally, the present paper points out that if we add the last two points to the hypothesis and keep in mind that mast cells have been known very recently to be versatile regulators of inflammation, tissue remodeling, host defense and homeostasis, the rich pathophysiological functions of the meridian pointed out by the Chinese medicine can be understood quite naturally.
	TO cite this article:Weisheng Yang. Investigation of the Lower Resistance Meridian V. Speculation on the Pathophysiological Functions of Acupuncture Meridians[OL].[18 November 2008] http://en.paper.edu.cn/en_releasepaper/content/25792


	5. Research advance of safflower on chemical composition
	Li Junfu,Yin Rongli,Wei Juan,Li Xiaoyi,Luo Yan
	Traditional Chinese Medicine and Pharmacology 22 September 2008
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	Abstract:Safflower,as a plant of Carthamus L.in compositae,is one of blood-activating and stasis-eliminating Chinese medicines,Safflower Yellow is mainly its active component. Modern studies shows that Safflower has significant effects on Cardiocerebral Vascular Diseases This text overviews the reseach advance of safflower from chemical composition, pharmacologic action,pharmacokineticcs and toxicology respectively.
	TO cite this article:Li Junfu,Yin Rongli,Wei Juan, et al. Research advance of safflower on chemical composition[OL].[22 September 2008] http://en.paper.edu.cn/en_releasepaper/content/24204


	6. Fingerprint analysis of different Panax herbal species by HPLC-UV method
	Feng Zeng,Wang Xiaoming,Yang Min,Lu Zhiqiang,Guo Dean
	Traditional Chinese Medicine and Pharmacology 15 December 2007
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	Abstract:To establish a method for distinguishing different commercial samples of Panax species including notoginseng, cultivated ginseng (Chinese ginseng and Korean ginseng), wild ginseng, red ginseng and three types of American ginsengs and one American ginseng preparation with their HPLC fingerprints, so as to assure the quality of different commercial samples of Panax species, HPLC-UV method was used to establish their fingerprints; Zorbax Extend C18 (250mm×4.6 mm , 5μm) was used as analytical column, and acetonitrile/ KH2PO4 aqueous solution was used as mobile phase with gradient elution. The resultes showed that the fingerprints of different commercial samples of Panax species were variant in their holistic chromatograms and some specific constituents. The method is reliable, reproducible and simple, and can be used as an analytical method for the routine material discrimination of different commercial samples of Panax species.
	TO cite this article:Feng Zeng,Wang Xiaoming,Yang Min, et al. Fingerprint analysis of different Panax herbal species by HPLC-UV method[OL].[15 December 2007] http://en.paper.edu.cn/en_releasepaper/content/16989


	7. Induction of Apoptosis and G2/M Cell Cycle Arrest by Oridonin in Human Gastric Cancer BGC-823 Cells
	Jian Han,Ye Min,Qiao Xue,Wu Wanying,Qu Guiqin,Guo Dean
	Traditional Chinese Medicine and Pharmacology 14 December 2007
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	Abstract:The in vitro apoptosis-induction effects of oridonin on gastric tumor cells BGC-823 and its effects on cell cycle, mitochondrial membrane potential and intracellular Ca2+ were investigated to shed light on the mode of its anticancer action. The results showed that oridonin inhibited BGC-823 cells growth with IC50 of 22.21 μM. It induced apoptosis in a dose-dependent manner. In addition, it could decrease mitochondria membrane potential, increase intracellular Ca2+, and activate pro-caspase 3. BGC-823 cells were arrested in G2/M cell cycle phase with lower expression of cyclin A protein. The up-regulation of p53 was observed before apoptosis occurred and cell cycle arrest. It suggested that oridonin inhibits the proliferation of BGC-823 cells through G2/M cell cycle arrest and apoptosis induction, which is mediated by influx of Ca2+, up-regulation of p53, activation of caspase-3, and down-regulation of cyclin A.
	TO cite this article:Jian Han,Ye Min,Qiao Xue, et al. Induction of Apoptosis and G2/M Cell Cycle Arrest by Oridonin in Human Gastric Cancer BGC-823 Cells[OL].[14 December 2007] http://en.paper.edu.cn/en_releasepaper/content/16926


	8. Carbon nanotube-nujol paste electrode for the determination of quercetin
	He Jianbo,Xu Hongmei,Zhu Yanwu
	Traditional Chinese Medicine and Pharmacology 06 October 2004
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	Abstract:Adsorptive-stripping chronopotentiometry and voltammetry were used to study the adsorption and oxidation of quercetin at both nujol-graphite paste electrode (GPE) and nujol-carbon nanotubes paste electrode (CNTPE) for the possible application of quercetin determination. As compared with GPE, CNTPE showed very great power to adsorb quercetin and resulted in a considerable signals enhancement. The adsorption isotherm of quercetin on CNTPE was of Langmuir type, and the stripping of quercetin adsorbed on CNTs showed a quasi-reversible oxidation reaction. The high adsorbtive activity of CNTPE was contributed to the high specific surface area and the special surface characteristics of carbon nanotubes. The differential pulse voltammetry showed that peak current response depended linearly on quercetin concentration. A linear equation Ip/mA = 0.987c/mmoldm–3 + 0.023 with a correlation coefficient of 0.994 was obtained over the concentration range 0.1–1.0mmoldm–3.
	TO cite this article:He Jianbo,Xu Hongmei,Zhu Yanwu. Carbon nanotube-nujol paste electrode for the determination of quercetin[OL].[ 6 October 2004] http://en.paper.edu.cn/en_releasepaper/content/1125

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