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	1. Antiplatelet Profiles of Six Water Extracts from Medicinal Herbs for Activating Blood and Resolving Stasis
	TAO Li,ZHANG Lei,RUAN Junshan,YAN Linggeng,LU Yin
	Traditional Chinese Medicine and Pharmacology 05 December 2013
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	Abstract:Traditional Chinese medicine (TCM) for activating blood and resolving stasis (Huoxuehuayu) has been widely applied in treatment of various platelet-related diseases. The present study was to explore the antiplatelet activity of the water extracts from six kinds of Huoxuehuayu herbs commonly used in clinical context, which fall into three categories, namely Hexue, Huoxue, and Poxue. We also investigated the differences in the antiplatelet activity of the water extracts from three categories. To this end, human platelet aggregation assay induced by ADP and thrombin was used to evaluate the antiplatelet activity, and flow cytometry assay for detecting the effects on the expression of CD41 and CD62P, two activated platelet surface markers. Results showed that all the water extracts (1 mg/ml) could significantly suppress human platelet aggregation in vitro (P<0.01), but extracts from three categories exhibited different inhibitory effects on expression of CD41 and CD62P respectively, suggesting the different properties for Huoxuehuayu. We concluded that combined use of Huoxuehuayu herbs may have potent therapeutic effects against platelet-related diseases in clinical contexts.
	TO cite this article:TAO Li,ZHANG Lei,RUAN Junshan, et al. Antiplatelet Profiles of Six Water Extracts from Medicinal Herbs for Activating Blood and Resolving Stasis[OL].[ 5 December 2013] http://en.paper.edu.cn/en_releasepaper/content/4573339


	2. Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in beer, red wine and grape juice
	CAO Jiliang,KONG Weijun,ZHOU Shujun,YIN Lihui,Wan Li,YANG Meihua
	Traditional Chinese Medicine and Pharmacology 17 January 2013
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	Abstract:A simple, reliable and low-cost method based on molecularly imprinted polymer as selective sorbent of solid-phase extraction was proposed for the determination of ochratoxin A in beer, red wine and grape juice by HPLC coupled with fluorescence detection (HPLC-FLD). Samples were diluted with water and cleaned up with an AFFINIMIP? SPE OTA column. After washing and eluting, the analyte was analyzed by HPLC-FLD. Under the optimized conditions, the limit of detection (LOD) and limit of quantification (LOQ) for ochratoxin A were 0.025 ng mL?1 and 0.08 ng mL-1, respectively. The recoveries of ochratoxin A from beer, red wine and grape spiked at 0.1, 2 and 5 ng mL-1 ranged from 91.6 to 101.7%. Furthermore, after a simple regenerated procedure, the MIP-based SPE column could be reused at least fourteen times to achieve more than 80% recoveries of ochratoxin A in real samples. The developed method was applied to the detection of thirty beer, red wine and grape juice samples and just only four samples were contaminated by ochratoxin A with levels below the legal limits.
	TO cite this article:CAO Jiliang,KONG Weijun,ZHOU Shujun, et al. Molecularly imprinted polymer-based solid phase clean-up for analysis of ochratoxin A in beer, red wine and grape juice[J].


	3. Six cytoxic annonaceous acetogenins from Annona squamosa seeds
	CHEN Yong,CHEN Jianwei,WANG Yu,XU Shasha,LI Xiang
	Traditional Chinese Medicine and Pharmacology 16 May 2012
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	Abstract:Custard apple (Annona squamosa L.) is an edible tropical fruit, and its seeds had been used in south China as folk medicine to treat "malignant sore" (cancer) and other usage as insecticide. Phytochemical investigation of the ethanol fraction of custard apple seeds led to the isolation of six new annonaceous acetogenins, annosquacins A-D (1-4), annosquatin A (5) and annosquatin B (6). Their structures were elucidated by spectroscopic analysis. Compounds 1-4 are adjacent bistetrahydrofuran annonaceous acetogenins. Compounds 5 and 6 are non-adjacent bistetrahydrofuran annonaceous acetogenins and the first examples in which the tetrahydrofuran ring system is located between C-9 and C-20. The absolute configurations of 1-6 were defined by the application of the Mosher method. Compounds 1-6 exhibited potent cytotoxic activity in vitro against five human tumor cell lines. 5 and 6 showed a high selectivity toward the MCF-7 and A-549 cell line respectively.
	TO cite this article:CHEN Yong,CHEN Jianwei,WANG Yu, et al. Six cytoxic annonaceous acetogenins from Annona squamosa seeds[OL].[16 May 2012] http://en.paper.edu.cn/en_releasepaper/content/4478772


	4. Preparation and Characterization of Resveratrol Molecularly Imprinted Polymeric Microspheres
	Hong Meihua,Chen Yuantao
	Traditional Chinese Medicine and Pharmacology 25 June 2010
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	Abstract:The molecularly imprinted polymeric microspheres (MIPMs) have been successfully prepared by a single-step swelling and polymerization in polar solvent using the polystyrene particles as seeds, and resveratrol as template molecule. The resveratrol-MIPMs were characterized with scanning electronic microscope. The synthetic condition and the adsorption properties were also studied. It was found that the microspheres with concave-dipression structure had large surface area and lots of binding sites, effectively raising the combination rate and imprinted capacity.
	TO cite this article:Hong Meihua,Chen Yuantao. Preparation and Characterization of Resveratrol Molecularly Imprinted Polymeric Microspheres[OL].[25 June 2010] http://en.paper.edu.cn/en_releasepaper/content/4377209

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